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Protein Page:
H2AFY (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
H2AFY Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post- translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP- ribose, and may be involved in ADP-ribose-mediated chromatin modulation. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. Interacts with SPOP. Part of a complex consisting of H2AFY, CUL3 and SPOP. Interacts with HDAC1 and HDAC2. Ubiquitous. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: DNA binding protein
Cellular Component: sex chromatin; ESC/E(Z) complex; nuclear chromatin; nucleolus; condensed chromosome; Barr body; nucleosome; nucleus
Molecular Function: rDNA binding; protein serine/threonine kinase inhibitor activity; protein binding; DNA binding; double-stranded methylated DNA binding; chromatin DNA binding; protein heterodimerization activity; protein kinase binding
Biological Process: nucleosome assembly; negative regulation of gene expression, epigenetic; dosage compensation; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; regulation of cell growth; negative regulation of histone phosphorylation
Reference #:  O75367 (UniProtKB)
Alt. Names/Synonyms: Core histone macro-H2A.1; H2A histone family, member Y; H2A.y; H2A/y; H2AF12M; H2AFJ; H2AFY; H2AY; Histone H2A.y; Histone macroH2A1; histone macroH2A1.1; histone macroH2A1.2; MACROH2A1; MACROH2A1.1; macroH2A1.2; Medulloblastoma antigen MU-MB-50.205; mH2A1
Gene Symbols: H2AFY
Molecular weight: 39,617 Da
Basal Isoelectric point: 9.8  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

H2AFY

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 K7-ac _MSSRGGkKKSTkTS
0 1 K12-ac GGkKKSTkTSRSAkA
0 1 K18-ac TkTSRSAkAGVIFPV
0 1 K18-m1 TkTSRSAkAGVIFPV
0 1 K18-ub TkTSRSAkAGVIFPV
0 4 K96-ub EELNQLLkGVTIAsG
0 1 S102-p LkGVTIAsGGVLPNI
1 137 K116-ub IHPELLAkkRGskGk
1 87 K117-ub HPELLAkkRGskGkL
0 1 S120-p LLAkkRGskGkLEAI
0 29 K121-ub LAkkRGskGkLEAII
0 1 K123-m2 kkRGskGkLEAIItP
0 109 K123-ub kkRGskGkLEAIItP
0 31 T129-p GkLEAIItPPPAKKA
1 4 S138-p PPAKKAKsPsQKKPV
0 2 S140-p AKKAKsPsQKKPVsK
0 1 S146-p PsQKKPVsKKAGGKk
0 1 K153-ac sKKAGGKkGARKskK
0 1 S158-p GKkGARKskKKQGEV
0 1 K159-ac KkGARKskKKQGEVs
0 1 S166-p kKKQGEVskAAsAds
0 30 K167-ub KKQGEVskAAsAdst
0 22 S170-p GEVskAAsAdsttEG
0 1 D172-ca VskAAsAdsttEGtP
0 14 S173-p skAAsAdsttEGtPA
0 6 T174-p kAAsAdsttEGtPAD
0 8 T175-p AAsAdsttEGtPADG
0 9 T178-p AdsttEGtPADGFTV
0 3 A180 sttEGtPADGFTVLS
0 5 K189-ub GFTVLSTkSLFLGQk
0 1 K196-ub kSLFLGQkLNLIHSE
0 1 S202 QkLNLIHSEISNLAG
0 1 S205 NLIHSEISNLAGFEV
0 1 K235 DLGNTLEKKGGkEFV
0 1 K235-ub DLGNTLEkKGGkEFV
0 1 K239-m1 TLEkKGGkEFVEAVL
0 1 K239-m2 TLEkKGGkEFVEAVL
0 2 K251-ub AVLELRKkNGPLEVA
0 4 K285-ub SPVWGADkCEELLEk
0 47 K292-ub kCEELLEkTVkNCLA
0 8 K295-ub ELLEkTVkNCLALAD
0 7 K304-ac CLALADDkKLksIAF
0 1 K304-ub CLALADDkKLksIAF
0 2 K307-ub LADDkKLksIAFPsI
0 1 S308-p ADDkKLksIAFPsIG
0 1 S313-p LksIAFPsIGsGRNG
0 2 S316-p IAFPsIGsGRNGFPk
0 1 K323-ub sGRNGFPkQtAAQLI
0 1 T325-p RNGFPkQtAAQLILK
0 1 Y362-p DSESIGIyVQEMAKL
  mouse

 
K7 _MSSRGGKKKSTKTS
K12 GGKKKSTKTSRSAKA
K18 TKTSRSAKAGVIFPV
K18 TKTSRSAKAGVIFPV
K18 TKTSRSAKAGVIFPV
K96-ub EELNQLLkGVTIASG
S102 LkGVTIASGGVLPNI
K116-ub IHPELLAkkRGSkGk
K117-ub HPELLAkkRGSkGkL
S120 LLAkkRGSkGkLEAI
K121-ub LAkkRGSkGkLEAII
K123 kkRGSkGKLEAIItP
K123-ub kkRGSkGkLEAIItP
T129-p GkLEAIItPPPAKKA
S138-p PPAKKAKsPSQKKPV
S140 AKKAKsPSQKKPVAK
A146 PSQKKPVAKKTGGKK
K153 AKKTGGKKGARKSKK
S158 GKKGARKSKKKQGEV
K159 KKGARKSKKKQGEVS
S166 KKKQGEVSkAAsADs
K167-ub KKQGEVSkAAsADst
S170-p GEVSkAAsADsttEG
D172 VSkAAsADsttEGtP
S173-p SkAAsADsttEGtPt
T174-p kAAsADsttEGtPtD
T175-p AAsADsttEGtPtDG
T178-p ADsttEGtPtDGFTV
T180-p sttEGtPtDGFTVLS
K189-ub GFTVLSTkSLFLGQK
K196 kSLFLGQKLNLIHsE
S202-p QKLNLIHsEIsNLAG
S205-p NLIHsEIsNLAGFEV
K235-ac DLGNTLEkKGGKEFV
K235 DLGNTLEKKGGKEFV
K239 TLEkKGGKEFVEAVL
K239 TLEkKGGKEFVEAVL
K251 AVLELRKKNGPLEVA
K285-ub SPVWGADkCEELLEk
K292-ub kCEELLEkTVkNCLA
K295-ub ELLEkTVkNCLALAD
R304 CLALADDRKLkSIAF
R304 CLALADDRKLkSIAF
K307-ub LADDRKLkSIAFPSI
S308 ADDRKLkSIAFPSIG
S313 LkSIAFPSIGSGRNG
S316 IAFPSIGSGRNGFPK
K323 SGRNGFPKQTAAQLI
T325 RNGFPKQTAAQLILK
Y362 DSESIGIYVQEMAKL
  rat

 
K7 _MSSRGGKKKSTKTS
K12 GGKKKSTKTSRSAKA
K18 TKTSRSAKAGVIFPV
K18 TKTSRSAKAGVIFPV
K18 TKTSRSAKAGVIFPV
K96 EELNQLLKGVTIASG
S102 LKGVTIASGGVLPNI
K116-ub IHPELLAkkRGSKGK
K117-ub HPELLAkkRGSKGKL
S120 LLAkkRGSKGKLEAI
K121 LAkkRGSKGKLEAII
K123 kkRGSKGKLEAIITP
K123 kkRGSKGKLEAIITP
T129 GKLEAIITPPPAKKA
S138 PPAKKAKSPSQKKPV
S140 AKKAKSPSQKKPVAK
A146 PSQKKPVAKKTGGKK
K153 AKKTGGKKGARKSKK
S158 GKKGARKSKKQGEVS
K159 KKGARKSKKQGEVSK
S165 SKKQGEVSKAAsADs
K166 KKQGEVSKAAsADsT
S169-p GEVSKAAsADsTTEG
D171 VSKAAsADsTTEGAP
S172-p SKAAsADsTTEGAPT
T173 KAAsADsTTEGAPTD
T174 AAsADsTTEGAPTDG
A177 ADsTTEGAPTDGFTV
T179 sTTEGAPTDGFTVLS
K188 GFTVLSTKSLFLGQK
K195 KSLFLGQKLNLIHSE
S201 QKLNLIHSEISNLAG
S204 NLIHSEISNLAGFEV
K234 DLGSTLEKKGGKEFV
K234 DLGSTLEKKGGKEFV
K238 TLEKKGGKEFVEAVL
K238 TLEKKGGKEFVEAVL
K250 AVLELRKKNGPLEVA
K284 SPVWGADKCEELLEK
K291 KCEELLEKTVKNCLA
K294 ELLEKTVKNCLALAD
R303 CLALADDRKLKSIAF
R303 CLALADDRKLKSIAF
K306 LADDRKLKSIAFPSI
S307 ADDRKLKSIAFPSIG
S312 LKSIAFPSIGSGRNG
S315 IAFPSIGSGRNGFPK
K322 SGRNGFPKQTAAQLI
T324 RNGFPKQTAAQLILK
Y361 DSESIGIYVQEMAKL
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