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Protein Page:
APRIN (rat)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
g O-GlcNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
APRIN a widely expressed nuclear protein. Is lost in many cancers and may function as a tumor suppressor. Correlates with differentiation in embryonal carcinoma stem cells; its knockdown disrupted Nanog, Oct4, and SOX2 regulation. Plays a role in androgen-induced proliferative arrest in prostate cells. Is upregulated in androgen-sensitive LNCaP prostate cancer cells undergoing androgen-induced proliferative block. Required for maintenance of sister chromatid cohesion during mitosis. May couple sister chromatid cohesion during mitosis to DNA replication. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Belongs to the PDS5 family. Induced by the synthetic androgen R1881 in prostate carcinoma cells undergoing proliferative arrest. Phosphorylated upon DNA damage. Maximum levels occur 18-20 hours after androgen exposure. Interacts with the cohesin complex. Phosphorylated upon DNA damage, probably by ATM or ATR. Five isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Cell development/differentiation; DNA binding protein
Cellular Component: nucleolus; chromatin; nucleus
Molecular Function: DNA binding
Biological Process: negative regulation of cell proliferation; cell division; mitotic sister chromatid cohesion; regulation of cell proliferation
Reference #:  Q6TRW4 (UniProtKB)
Alt. Names/Synonyms: Androgen-induced proliferation inhibitor; androgen-induced prostate proliferative shutoff associated protein; Androgen-induced prostate proliferative shutoff-associated protein AS3; androgen-induced shutoff 3; Aprin; As3; CG008; FLJ23236; KIAA0979; PDS5, regulator of cohesion maintenance, homolog B (S. cerevisiae); Pds5b; Sister chromatid cohesion protein PDS5 homolog B
Gene Symbols: Pds5b
Molecular weight: 164,459 Da
Basal Isoelectric point: 8.73  Predict pI for various phosphorylation states
Select Structure to View Below

APRIN
Protein Structure Not Found.


STRING  |  Scansite  |  Phospho.ELM  |  Pfam  |  UniProtKB  |  Entrez-Gene  |  GenPept


Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


  MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


        rat

 
0 1 K12 KTRTNDGKITYPPGV
0 1 K20 ITYPPGVKEISDKIS
0 1 T41 RLKMVVKTFMDMDQD
0 1 K53 DQDSEEEKELYLNLA
0 1 T98 YAPEAPYTSPDKLKD
0 1 K212 PAHKNLNKQAYDLAK
0 2 Y215 KNLNKQAYDLAKALL
0 1 K219 KQAYDLAKALLKRTA
0 1 S252 KTSISDLSEHVFDLI
0 1 K349 MNHPDLAKDLTEYLK
0 2 Y419 LAQIYKKYALQSAAG
0 1 K507 NLLRHQVKDLLDLIK
0 1 T533 FSKVMVITRNLPDPG
0 1 Y728 GPPRQAKYAIHCIHA
0 1 S787 QFAAPLKSLVATFIV
0 1 S844 LGMKNNHSKSGTSTL
0 1 K1089 TYSLESPKDPVLPAR
0 10 K1103 RFFTQPDKNFSNTKN
0 22 T1121 PEMKSFFTPGKPKTA
0 1 K1136 NVLGAVNKPLSSAGK
0 1 S1139 GAVNKPLSSAGKQSQ
0 2 S1140 AVNKPLSSAGKQSQT
0 3 S1145 LSSAGKQSQTKSSRM
0 1 T1147 SAGKQSQTKSSRMET
0 1 S1160 ETVSNASSSSNPssP
0 1 S1161 TVSNASSSSNPssPG
0 10 S1162 VSNASSSSNPssPGR
0 18 S1165-p ASSSSNPssPGRIKG
0 115 S1166-p SSSSNPssPGRIKGR
0 478 S1176 RIKGRLDSTEMDHSE
0 331 T1177 IKGRLDSTEMDHSEN
0 563 S1182 DSTEMDHSENEDYTM
0 136 Y1187 DHSENEDYTMSSPLP
0 33 T1188 HSENEDYTMSSPLPG
0 37 S1190 ENEDYTMSSPLPGKK
0 64 S1191 NEDYTMSSPLPGKKS
0 1 S1209 EDSDLVRSELEKPRS
0 2 A1220 KPRSRKKASVTDPEE
0 1 K1228 SVTDPEEKLGMDDLS
0 2 S1235 KLGMDDLSKLVQEQK
0 12 S1246 QEQKPKGSQRGRKRG
0 11 A1255 RGRKRGHAAsESEEQ
0 31 S1257-p RKRGHAAsESEEQQW
0 25 S1259 RGHAAsESEEQQWPE
0 26 S1283 GNEDEQNSPPKKGKR
0 3 T1300 PPKPLGGTSKEEPVV
0 2 P1318 KKGNKKKPAPPVVDE
0 1 M1333 DEEEERQMGNTEQKS
0 5 S1348 KSKQQRTSKRAQQRA
0 106 S1357-p RAQQRAEsPETSAVE
0 7 T1360 QRAEsPETSAVESTQ
0 9 S1361 RAEsPETSAVESTQS
0 7 S1365 PETSAVESTQSTPQK
0 5 T1366 ETSAVESTQSTPQKG
1 17 S1368 SAVESTQSTPQKGRG
2 72 T1369 AVESTQSTPQKGRGR
0 1 S1378 QKGRGRPSKtPsPSQ
0 4 T1380-p GRGRPSKtPsPSQPK
0 11 S1382-p GRPSKtPsPSQPKKN
0 3 S1384 PSKtPsPSQPKKNIR
0 2 S1406 ATKENDSSEEMDVLQ
0 1 S1415 EMDVLQASSPVSDDT
0 3 S1416 MDVLQASSPVSDDTT
0 1 S1419 LQASSPVSDDTTQEG
0 22 S1441-p AGNVRRRsSKRERR_
  human

 
K48-a KTRTNDGkITYPPGV
K56-a ITYPPGVkEISDKIS
T77-p RLKMVVKtFMDMDQD
K89-a DQDSEEEkELYLNLA
T134-p YAPEAPYtSPDKLKD
K248-a PAHKNLNkQAyDLAk
Y251-p KNLNkQAyDLAkALL
K255-a kQAyDLAkALLKRTA
S288 KTSISDLSEHVFDLI
K385-u MNHPDLAkDLTEYLK
Y455-p LAQIYKKyALQSAAG
K543-u NLLRHQVkDLLDLIK
T569 FSKVMVITRNLPDPG
Y764-p GPPRQAKyAIHCIHA
S823-p QFAAPLKsLVATFIV
S880-p LGMKNNHsKSGTSTL
K1125 TYSLESPKDPVLPAR
K1139-a RFFTQPDkNFSNTKN
T1157-p PEMKSFFtPGKPKTT
K1172-a NVLGAVNkPLssAGK
S1175-p GAVNkPLssAGKQsQ
S1176-p AVNkPLssAGKQsQt
S1181-p LssAGKQsQtKSSRM
T1183-p sAGKQsQtKSSRMET
S1196-p ETVSNASsSsNPssP
S1197 TVSNASsSsNPssPG
S1198-p VSNASsSsNPssPGR
S1201-p ASsSsNPssPGRIKG
S1202-p SsSsNPssPGRIKGR
S1212-p RIKGRLDssEMDHsE
S1213-p IKGRLDssEMDHsEN
S1218-p DssEMDHsENEDytM
Y1223-p DHsENEDytMssPLP
T1224-p HsENEDytMssPLPG
S1226-p ENEDytMssPLPGKK
S1227-p NEDytMssPLPGKKS
S1245 DDSDLVRSELEKPRG
T1256-p KPRGRKKtPVTEQEE
K1264-a PVTEQEEkLGMDDLt
T1271-p kLGMDDLtKLVQEQK
S1282-p QEQKPKGsQRSRKRG
T1291-p RSRKRGHtAsEsDEQ
S1293-p RKRGHtAsEsDEQQW
S1295-p RGHtAsEsDEQQWPE
S1319-p ENEDEQNsPPKKGKR
T1337-p PKPLGGGtPKEEPTM
S1355-p KKGSKKKsGPPAPEE
S1370-p EEEEERQsGNTEQKS
S1385-p KSKQHRVsRRAQQRA
S1394-p RAQQRAEsPEssAIE
S1397-p QRAEsPEssAIEstQ
S1398-p RAEsPEssAIEstQs
S1402-p PEssAIEstQstPQK
T1403-p EssAIEstQstPQKG
S1405-p sAIEstQstPQKGRG
T1406-p AIEstQstPQKGRGR
S1415-p QKGRGRPsKtPsPsQ
T1417-p GRGRPsKtPsPsQPK
S1419-p GRPsKtPsPsQPKKN
S1421-p PsKtPsPsQPKKNVR
S1443-p ATKENDSsEEVDVFQ
S1452-p EVDVFQGssPVDDIP
S1453-p VDVFQGssPVDDIPQ
- gap
S1477-p TVNVRRRsAKRERR_
  mouse

 
K12 KTRTNDGKITYPPGV
K20 ITYPPGVKEISDKIS
T41 RLKMVVKTFMDMDQD
K53 DQDSEEEKELYLNLA
T98 YAPEAPYTSPDKLKD
K212 PAHKNLNKQAYDLAK
Y215 KNLNKQAYDLAKALL
K219 KQAYDLAKALLKRTA
S252-p KTSISDLsEHVFDLI
K349 MNHPDLAKDLTEYLK
Y419 LAQIYKKYSLQSAAG
K507 NLLRHQVKDLLDLIK
T533-p FSKVMVItRNLPDPG
Y728 GPPRQAKYAIHCIHA
S787 QFAAPLKSLVATFIV
S844 LGMKNNHSKSGTSTL
K1089-u TYSLESPkDPVLPAR
K1103 RFFTQPDKNFSNTKN
T1121-p PEMKSFFtPGKPKTA
K1136 NVLGAVNKPLSSAGK
S1139 GAVNKPLSSAGKQSQ
S1140 AVNKPLSSAGKQSQT
S1145 LSSAGKQSQTKSSRM
T1147 SAGKQSQTKSSRMET
S1160-p ETVSNASsssNPssP
S1161-p TVSNASsssNPssPG
S1162-p VSNASsssNPssPGR
S1165-p ASsssNPssPGRIKG
S1166-p SsssNPssPGRIKGR
S1176-p RIKGRLDssEMDHsE
S1177-p IKGRLDssEMDHsEN
S1182-p DssEMDHsENEDytM
Y1187-p DHsENEDytMssPLP
T1188-p HsENEDytMssPLPG
S1190-p ENEDytMssPLPGKK
S1191-p NEDytMssPLPGKKS
S1207-p KREDPDLsELEKPRS
A1218 KPRSRKKAPVTDPEE
K1226 PVTDPEEKLGMDDLT
T1233 KLGMDDLTKLVQEQK
S1244-p QEQKPKGsQRGRKRG
T1253-p RGRKRGRtAsDsDEQ
S1255-p RKRGRtAsDsDEQQW
S1257-p RGRtAsDsDEQQWPE
S1281-p ENEDEQNsPPKKGKR
T1299 PKPLGGGTSKEEPTM
L1317 KKGNKKKLVPPVVDD
I1332 DEEEERQIGNTEHKS
S1347 KSKQHRTSKRAQQRA
S1356-p RAQQRAEsPEtsAVE
T1359-p QRAEsPEtsAVEstQ
S1360-p RAEsPEtsAVEstQs
S1364-p PEtsAVEstQstPQK
T1365-p EtsAVEstQstPQKG
S1367-p sAVEstQstPQKGRG
T1368-p AVEstQstPQKGRGR
S1377 QKGRGRPSKAPsPsQ
A1379 GRGRPSKAPsPsQPP
S1381-p GRPSKAPsPsQPPKK
S1383-p PSKAPsPsQPPKKIR
S1405-p ATKENDSsEEMDVLQ
S1414 EMDVLQASsPVsDDT
S1415-p MDVLQASsPVsDDTT
S1418-p LQASsPVsDDTTQEG
S1440-p VGNVRRRsSKRERR_
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