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Protein Page:
APRIN (rat)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
APRIN a widely expressed nuclear protein. Is lost in many cancers and may function as a tumor suppressor. Correlates with differentiation in embryonal carcinoma stem cells; its knockdown disrupted Nanog, Oct4, and SOX2 regulation. Plays a role in androgen-induced proliferative arrest in prostate cells. Is upregulated in androgen-sensitive LNCaP prostate cancer cells undergoing androgen-induced proliferative block. Required for maintenance of sister chromatid cohesion during mitosis. May couple sister chromatid cohesion during mitosis to DNA replication. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Belongs to the PDS5 family. Induced by the synthetic androgen R1881 in prostate carcinoma cells undergoing proliferative arrest. Phosphorylated upon DNA damage. Maximum levels occur 18-20 hours after androgen exposure. Interacts with the cohesin complex. Phosphorylated upon DNA damage, probably by ATM or ATR. Five isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Cell development/differentiation; DNA binding protein
Cellular Component: nucleolus; chromatin; nucleus
Molecular Function: DNA binding
Biological Process: negative regulation of cell proliferation; mitotic sister chromatid cohesion; regulation of cell proliferation
Reference #:  Q6TRW4 (UniProtKB)
Alt. Names/Synonyms: Androgen-induced proliferation inhibitor; androgen-induced prostate proliferative shutoff associated protein; Androgen-induced prostate proliferative shutoff-associated protein AS3; androgen-induced shutoff 3; Aprin; As3; CG008; FLJ23236; KIAA0979; PDS5, regulator of cohesion maintenance, homolog B (S. cerevisiae); Pds5b; Sister chromatid cohesion protein PDS5 homolog B
Gene Symbols: Pds5b
Molecular weight: 164,459 Da
Basal Isoelectric point: 8.73  Predict pI for various phosphorylation states
Select Structure to View Below

APRIN

Protein Structure Not Found.


STRING  |  Reactome  |  BioGPS  |  Scansite  |  Pfam  |  Phospho.ELM  |  UniProtKB  |  Entrez-Gene  |  GenPept


Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       rat

 
0 1 K12 KTRTNDGKITYPPGV
0 1 K20 ITYPPGVKEISDKIS
0 1 T41 RLKMVVKTFMDMDQD
0 1 K53 DQDSEEEKELYLNLA
0 1 T98 YAPEAPYTSPDKLKD
0 1 T111 KDIFMFITRQLKGLE
0 1 K212 PAHKNLNKQAYDLAK
0 3 Y215 KNLNKQAYDLAKALL
0 1 K219 KQAYDLAKALLKRTA
0 1 S252 KTSISDLSEHVFDLI
0 1 K349 MNHPDLAKDLTEYLK
0 2 Y419 LAQIYKKYALQSAAG
0 1 K507 NLLRHQVKDLLDLIK
0 1 T518 DLIKQPKTDASVKAI
0 2 T533 FSKVMVITRNLPDPG
0 1 Y728 GPPRQAKYAIHCIHA
0 1 S787 QFAAPLKSLVATFIV
0 1 S844 LGMKNNHSKSGTSTL
0 1 S985 AVSEKLLSLLPEYVV
0 1 Y990 LLSLLPEYVVPYTIH
0 1 Y1005 LLAHDPDYVKVQDIE
0 1 - under review  
0 1 S1087 STTYSLESPKDPVLP
0 1 K1089 TYSLESPKDPVLPAR
0 10 K1103 RFFTQPDKNFSNTKN
0 1 S1118 YLPPEMKSFFTPGKP
0 27 T1121 PEMKSFFTPGKPKTA
0 2 K1136-ac NVLGAVNkPLSSAGK
0 3 S1139 GAVNkPLSSAGKQSQ
0 5 S1140 AVNkPLSSAGKQSQT
0 4 S1145 LSSAGKQSQTKSSRM
0 2 T1147 SAGKQSQTKSSRMET
0 2 T1154 TKSSRMETVSNASSS
0 1 S1156 SSRMETVSNASSSsN
0 1 S1159 METVSNASSSsNPss
0 3 S1160 ETVSNASSSsNPssP
0 1 S1161 TVSNASSSsNPssPG
0 17 S1162-p VSNASSSsNPssPGR
0 24 S1165-p ASSSsNPssPGRIKG
0 127 S1166-p SSSsNPssPGRIKGR
0 498 S1176 RIKGRLDStEMDHsE
0 338 T1177-p IKGRLDStEMDHsEN
0 586 S1182-p DStEMDHsENEDYTM
0 138 Y1187 DHsENEDYTMSSPLP
0 33 T1188 HsENEDYTMSSPLPG
0 37 S1190 ENEDYTMSSPLPGKK
0 67 S1191 NEDYTMSSPLPGKKS
0 2 S1204 KSDKREDSDLVRSEL
0 3 S1209 EDSDLVRSELEKPRS
0 4 A1220 KPRSRKKASVTDPEE
0 1 K1228 SVTDPEEKLGMDDLS
0 2 S1235 KLGMDDLSKLVQEQK
0 12 S1246 QEQKPKGSQRGRKRG
0 14 A1255 RGRKRGHAAsESEEQ
0 42 S1257-p RKRGHAAsESEEQQW
0 33 S1259 RGHAAsESEEQQWPE
0 37 S1283-p GNEDEQNsPPKKGKR
0 5 T1300 PPKPLGGTSKEEPVV
0 6 P1318 KKGNKKKPAPPVVDE
0 3 M1333 DEEEERQMGNTEQKS
0 5 S1348 KSKQQRTSKRAQQRA
0 118 S1357-p RAQQRAEsPETSAVE
0 10 T1360 QRAEsPETSAVESTQ
0 11 S1361 RAEsPETSAVESTQS
0 7 S1365 PETSAVESTQStPQK
0 5 T1366 ETSAVESTQStPQKG
1 17 S1368 SAVESTQStPQKGRG
2 81 T1369-p AVESTQStPQKGRGR
0 3 S1378 QKGRGRPSKtPsPSQ
0 7 T1380-p GRGRPSKtPsPSQPK
0 17 S1382-p GRPSKtPsPSQPKKN
0 4 S1384 PSKtPsPSQPKKNIR
0 4 S1406-p ATKENDSsEEMDVLQ
0 2 S1415-p EMDVLQAssPVsDDT
0 5 S1416-p MDVLQAssPVsDDTT
0 2 S1419-p LQAssPVsDDTTQEG
0 22 S1441-p AGNVRRRsSKRERR_
  human

 
K12-ac KTRTNDGkITYPPGV
K20-ac ITYPPGVkEISDKIS
T41-p RLKMVVKtFMDMDQD
K53-ac DQDSEEEkELYLNLA
T98-p YAPEAPYtSPDKLKD
T111-p KDIFMFItRQLKGLE
K212-ac PAHKNLNkQAyDLAk
Y215-p KNLNkQAyDLAkALL
K219-ac kQAyDLAkALLKRTA
S252 KTSISDLSEHVFDLI
K349-ub MNHPDLAkDLTEYLK
Y419-p LAQIYKKyALQSAAG
K507-ub NLLRHQVkDLLDLIK
T518-p DLIKQPKtDASVKAI
T533-p FSKVMVItRNLPDPG
Y728-p GPPRQAKyAIHCIHA
S787-p QFAAPLKsLVATFIV
S844-p LGMKNNHsKSGTSTL
S985-p AVSEKLLsLLPEyVV
Y990-p LLsLLPEyVVPYTIH
Y1005-p LLAHDPDyVKVQDIE
T1050-p MVENIKQtKDAQGPD
S1087-p STTYSLEsPKDPVLP
K1089 TYSLEsPKDPVLPAR
K1103-ac RFFTQPDkNFSNTKN
S1118-p YLPPEMKsFFtPGKP
T1121-p PEMKsFFtPGKPKTT
K1136-ac NVLGAVNkPLssAGK
S1139-p GAVNkPLssAGKQsQ
S1140-p AVNkPLssAGKQsQt
S1145-p LssAGKQsQtKSSRM
T1147-p sAGKQsQtKSSRMEt
T1154-p tKSSRMEtVsNAssS
S1156-p SSRMEtVsNAssSsN
S1159-p MEtVsNAssSsNPss
S1160-p EtVsNAssSsNPssP
S1161 tVsNAssSsNPssPG
S1162-p VsNAssSsNPssPGR
S1165-p AssSsNPssPGRIKG
S1166-p ssSsNPssPGRIKGR
S1176-p RIKGRLDssEMDHsE
S1177-p IKGRLDssEMDHsEN
S1182-p DssEMDHsENEDytM
Y1187-p DHsENEDytMssPLP
T1188-p HsENEDytMssPLPG
S1190-p ENEDytMssPLPGKK
S1191-p NEDytMssPLPGKKS
S1204-p KSDKRDDsDLVRsEL
S1209-p DDsDLVRsELEKPRG
T1220-p KPRGRKKtPVTEQEE
K1228-ac PVTEQEEkLGMDDLt
T1235-p kLGMDDLtKLVQEQK
S1246-p QEQKPKGsQRSRKRG
T1255-p RSRKRGHtAsEsDEQ
S1257-p RKRGHtAsEsDEQQW
S1259-p RGHtAsEsDEQQWPE
S1283-p ENEDEQNsPPKKGKR
T1301-p PKPLGGGtPKEEPTM
S1319-p KKGSKKKsGPPAPEE
S1334-p EEEEERQsGNTEQKS
S1349-p KSKQHRVsRRAQQRA
S1358-p RAQQRAEsPEssAIE
S1361-p QRAEsPEssAIEstQ
S1362-p RAEsPEssAIEstQs
S1366-p PEssAIEstQstPQK
T1367-p EssAIEstQstPQKG
S1369-p sAIEstQstPQKGRG
T1370-p AIEstQstPQKGRGR
S1379-p QKGRGRPsKtPsPsQ
T1381-p GRGRPsKtPsPsQPK
S1383-p GRPsKtPsPsQPKKN
S1385-p PsKtPsPsQPKKNVR
S1407-p ATKENDSsEEVDVFQ
S1416-p EVDVFQGssPVDDIP
S1417-p VDVFQGssPVDDIPQ
- gap
S1441-p TVNVRRRsAKRERR_
  mouse

 
K12 KTRTNDGKITYPPGV
K20 ITYPPGVKEISDKIS
T41 RLKMVVKTFMDMDQD
K53 DQDSEEEKELYLNLA
T98 YAPEAPYTSPDKLKD
T111 KDIFMFITRQLKGLE
K212 PAHKNLNKQAyDLAK
Y215-p KNLNKQAyDLAKALL
K219 KQAyDLAKALLKRTA
S252-p KTSISDLsEHVFDLI
K349 MNHPDLAKDLTEYLK
Y419 LAQIYKKYSLQSAAG
K507 NLLRHQVKDLLDLIK
T518 DLIKQPKTDASVKAI
T533-p FSKVMVItRNLPDPG
Y728 GPPRQAKYAIHCIHA
S787 QFAAPLKSLVATFIV
S844 LGMKNNHSKSGTSTL
S985 AVSEKLLSLLPEYVV
Y990 LLSLLPEYVVPYTIH
Y1005 LLAHDPDYVKVQDIE
T1050 MVENIKQTKDAQGPD
S1087 STTYSLESPkDPVLP
K1089-ub TYSLESPkDPVLPAR
K1103 RFFTQPDKNFSNTKN
S1118 YLPPEMKSFFtPGKP
T1121-p PEMKSFFtPGKPKTA
K1136-ac NVLGAVNkPLSSAGK
S1139 GAVNkPLSSAGKQSQ
S1140 AVNkPLSSAGKQSQT
S1145 LSSAGKQSQTKSSRM
T1147 SAGKQSQTKSSRMEt
T1154-p TKSSRMEtVSNASss
S1156 SSRMEtVSNASsssN
S1159 MEtVSNASsssNPss
S1160-p EtVSNASsssNPssP
S1161-p tVSNASsssNPssPG
S1162-p VSNASsssNPssPGR
S1165-p ASsssNPssPGRIKG
S1166-p SsssNPssPGRIKGR
S1176-p RIKGRLDssEMDHsE
S1177-p IKGRLDssEMDHsEN
S1182-p DssEMDHsENEDytM
Y1187-p DHsENEDytMssPLP
T1188-p HsENEDytMssPLPG
S1190-p ENEDytMssPLPGKK
S1191-p NEDytMssPLPGKKS
P1204 KSDKREDPDLsELEK
S1207-p KREDPDLsELEKPRS
A1218 KPRSRKKAPVTDPEE
K1226 PVTDPEEKLGMDDLT
T1233 KLGMDDLTKLVQEQK
S1244-p QEQKPKGsQRGRKRG
T1253-p RGRKRGRtAsDsDEQ
S1255-p RKRGRtAsDsDEQQW
S1257-p RGRtAsDsDEQQWPE
S1281-p ENEDEQNsPPKKGKR
T1299 PKPLGGGTSKEEPTM
L1317 KKGNKKKLVPPVVDD
I1332 DEEEERQIGNTEHKS
S1347 KSKQHRTSKRAQQRA
S1356-p RAQQRAEsPEtsAVE
T1359-p QRAEsPEtsAVEstQ
S1360-p RAEsPEtsAVEstQs
S1364-p PEtsAVEstQstPQK
T1365-p EtsAVEstQstPQKG
S1367-p sAVEstQstPQKGRG
T1368-p AVEstQstPQKGRGR
S1377 QKGRGRPSKAPsPsQ
A1379 GRGRPSKAPsPsQPP
S1381-p GRPSKAPsPsQPPKK
S1383-p PSKAPsPsQPPKKIR
S1405-p ATKENDSsEEMDVLQ
S1414 EMDVLQASsPVsDDT
S1415-p MDVLQASsPVsDDTT
S1418-p LQASsPVsDDTTQEG
S1440-p VGNVRRRsSKRERR_
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