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Protein Page:
GRP78 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
g O-GlcNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
GRP78 a member of the HSP family of molecular chaperones required for endoplasmic reticulum integrity and stress-induced autophagy. Plays a central role in regulating the unfolded protein response (UPR), and is an obligatory component of autophagy in mammalian cells.. May play an important role in cellular adaptation and oncogenic survival. One of the client proteins of GRP78 is protein double-stranded RNA-activated protein-like endoplasmic reticulum kinase (PERK). Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Note: This description may include information from UniProtKB.
Protein type: Chaperone
Cellular Component: signalosome; endoplasmic reticulum membrane; cell surface; endoplasmic reticulum lumen; endoplasmic reticulum; perinuclear region of cytoplasm; melanosome; ER-Golgi intermediate compartment; integral to endoplasmic reticulum membrane; midbody; nucleus
Molecular Function: protein binding, bridging; protein domain specific binding; protein binding; ubiquitin protein ligase binding; chaperone binding; ATPase activity; caspase inhibitor activity; unfolded protein binding; ribosome binding; misfolded protein binding; calcium ion binding; ATP binding
Biological Process: ATP catabolic process; platelet activation; ER-associated protein catabolic process; cerebellum structural organization; unfolded protein response; cerebellar Purkinje cell layer development; negative regulation of caspase activity; cellular response to glucose starvation; platelet degranulation; unfolded protein response, activation of signaling protein activity; positive regulation of protein ubiquitination; blood coagulation; negative regulation of transforming growth factor beta receptor signaling pathway; ER overload response; negative regulation of apoptosis
Reference #:  P11021 (UniProtKB)
Alt. Names/Synonyms: 78 kDa glucose-regulated protein; BiP; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; FLJ26106; GRP-78; GRP78; Heat shock 70 kDa protein 5; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); Heat-shock 70kD protein-5 (glucose-regulated protein, 78kD); HSPA5; Immunoglobulin heavy chain-binding protein; MIF2
Gene Symbols: HSPA5
Molecular weight: 72,333 Da
Basal Isoelectric point: 5.07  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

GRP78
Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


  MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


        human

 
0 1 S4-p ____MKLsLVAAMLL
0 1 S14-p AAMLLLLsAARAEEE
1 0 T37-p VVGIDLGtTySCVGV
0 1 Y39-p GIDLGtTySCVGVFK
0 1 K81 RLIGDAAKNQLTSNP
0 8 K81-u RLIGDAAkNQLTSNP
0 123 K96-a ENTVFDAkRLIGRTW
0 13 K96-u ENTVFDAkRLIGRTW
0 2 K113-a PSVQQDIkFLPFkVV
0 5 K113-u PSVQQDIkFLPFkVV
1 0 K118-a DIkFLPFkVVEkkTk
0 5 K118-u DIkFLPFkVVEkkTk
1 2 K122-a LPFkVVEkkTkPyIQ
1 0 K123-a PFkVVEkkTkPyIQV
0 1 K123-u PFkVVEkkTkPyIQV
0 1 T124 FkVVEkkTkPyIQVD
1 0 K125-a kVVEkkTkPyIQVDI
0 3 K125-u kVVEkkTkPyIQVDI
0 2 Y127-p VEkkTkPyIQVDIGG
1 0 K138-a DIGGGQTkTFAPEEI
0 1 K138-u DIGGGQTkTFAPEEI
0 1 S146-p TFAPEEIsAMVLTkM
1 0 K152-a IsAMVLTkMkETAEA
0 1 K152-u IsAMVLTkMkETAEA
1 0 K154-a AMVLTkMkETAEAyL
0 1 T156 VLTkMkETAEAyLGk
0 58 Y160-p MkETAEAyLGkKVTH
0 9 K163-a TAEAyLGkKVTHAVV
0 2 K163-u TAEAyLGkKVTHAVV
0 1 K164 AEAyLGkKVTHAVVT
0 3 K185-u DAQRQATkDAGtIAG
0 1 T189-p QATkDAGtIAGLNVM
0 1 T203-p MRIINEPtAAAIAYG
0 7 K213-u AIAYGLDkREGEKNI
1 0 T229-p VFDLGGGtFDVSLLT
0 4 K268-a RVMEHFIkLYKKKTG
0 1 K268-u RVMEHFIkLYKKKTG
0 1 K326 SETLTRAKFEELNMD
0 12 K326-u SETLTRAkFEELNMD
0 1 K340-a DLFRSTMkPVQKVLE
0 2 K340-u DLFRSTMkPVQKVLE
1 2 K352-a VLEDSDLkksDIDEI
0 10 K352-u VLEDSDLkksDIDEI
1 0 K353-a LEDSDLkksDIDEIV
0 1 K353-u LEDSDLkksDIDEIV
0 6 S354-p EDSDLkksDIDEIVL
0 16 K370-u GGSTRIPkIQQLVkE
1 5 K376-a PkIQQLVkEFFNGKE
0 2 K376-u PkIQQLVkEFFNGKE
0 1 T441-p TKLIPRNtVVPTKkS
0 4 K447-u NtVVPTKkSQIFSTA
0 11 K464-u NQPTVTIkVyEGERP
0 1 Y466-p PTVTIkVyEGERPLT
0 2 K474-u EGERPLTkDNHLLGT
0 1 T518-p VTAEDKGtGNKNkIT
0 20 K523-u KGtGNKNkITITNDQ
0 1 K547-a RMVNDAEkFAEEDKK
0 2 K547-u RMVNDAEkFAEEDKK
0 1 Y570-p NELESYAySLkNQIG
0 5 K573-u ESYAySLkNQIGDkE
0 1 K579-a LkNQIGDkEkLGGkL
0 1 K581-m3 NQIGDkEkLGGkLss
2 5 K585-a DkEkLGGkLssEDkE
0 21 K585-m1 DkEkLGGkLssEDkE
0 8 K585-m2 DkEkLGGkLssEDkE
1 5 K585-m3 DkEkLGGkLssEDkE
0 1 S587-p EkLGGkLssEDkETM
0 1 S588-p kLGGkLssEDkETME
0 1 K591-m2 GkLssEDkETMEKAV
0 4 K601-a MEKAVEEkIEWLESH
0 8 K601-u MEKAVEEkIEWLESH
0 6 K617-a DADIEDFkAkKKELE
0 2 K619-a DIEDFkAkKKELEEI
1 0 K633-a IVQPIISkLYGSAGP
0 1 T643 GSAGPPPTGEEDTAE
0 3 T648 PPTGEEDTAEkDEL_
0 2 A649 PTGEEDTAEkDEL__
0 1 K651-a GEEDTAEkDEL____
  mouse

 
T5 ___MMKFTVVAAALL
G15 AAALLLLGAVRAEEE
T38 VVGIDLGTTYSCVGV
Y40 GIDLGTTYSCVGVFK
K82-a RLIGDAAkNQLTSNP
K82-u RLIGDAAkNQLTSNP
K97-a ENTVFDAkRLIGRTW
K97-u ENTVFDAkRLIGRTW
K114 PSVQQDIKFLPFkVV
K114-u PSVQQDIkFLPFkVV
K119 DIkFLPFKVVEkKtK
K119-u DIkFLPFkVVEkKtK
K123-a LPFkVVEkKtKPyIQ
K124 PFkVVEkKtKPyIQV
K124 PFkVVEkKtKPyIQV
T125-p FkVVEkKtKPyIQVD
K126 kVVEkKtKPyIQVDI
K126 kVVEkKtKPyIQVDI
Y128-p VEkKtKPyIQVDIGG
K139 DIGGGQTKTFAPEEI
K139 DIGGGQTKTFAPEEI
S147 TFAPEEISAMVLTKM
K153 ISAMVLTKMKEtAEA
K153 ISAMVLTKMKEtAEA
K155 AMVLTKMKEtAEAyL
T157-p VLTKMKEtAEAyLGk
Y161-p MKEtAEAyLGkkVTH
K164-a tAEAyLGkkVTHAVV
K164-u tAEAyLGkkVTHAVV
K165-u AEAyLGkkVTHAVVT
K186-u DAQRQATkDAGTIAG
T190 QATkDAGTIAGLNVM
T204 MRIINEPTAAAIAYG
K214-u AIAYGLDkREGEKNI
T230 VFDLGGGTFDVSLLT
K269-a RVMEHFIkLYKKKTG
K269 RVMEHFIKLYKKKTG
K327-a SETLTRAkFEELNMD
K327-u SETLTRAkFEELNMD
K341 DLFRSTMKPVQKVLE
K341 DLFRSTMKPVQKVLE
K353-a VLEDSDLkKSDIDEI
K353 VLEDSDLKKSDIDEI
K354 LEDSDLkKSDIDEIV
K354 LEDSDLkKSDIDEIV
S355 EDSDLkKSDIDEIVL
K371-u GGSTRIPkIQQLVKE
K377 PkIQQLVKEFFNGKE
K377 PkIQQLVKEFFNGKE
T442 TKLIPRNTVVPTKkS
K448-u NTVVPTKkSQIFSTA
K465 NQPTVTIKVYEGERP
Y467 PTVTIKVYEGERPLT
K475 EGERPLTKDNHLLGT
T519 VTAEDKGTGNKNkIT
K524-u KGTGNKNkITITNDQ
K548 RMVNDAEKFAEEDKK
K548-u RMVNDAEkFAEEDKK
Y571 NELESYAYSLkNQIG
K574-u ESYAYSLkNQIGDKE
K580 LkNQIGDKEKLGGkL
K582 NQIGDKEKLGGkLSS
K586-a DKEKLGGkLSSEDKE
K586-m1 DKEKLGGkLSSEDKE
K586 DKEKLGGKLSSEDKE
K586 DKEKLGGKLSSEDKE
S588 EKLGGkLSSEDKETM
S589 KLGGkLSSEDKETME
K592 GkLSSEDKETMEKAV
K602 MEKAVEEKIEWLESH
K602 MEKAVEEKIEWLESH
K618-a DADIEDFkAkKKELE
K620-a DIEDFkAkKKELEEI
K634 IVQPIISKLYGSGGP
T644-p GSGGPPPtGEEDtsE
T649-p PPtGEEDtsEKDEL_
S650-p PtGEEDtsEKDEL__
K652 GEEDtsEKDEL____
  rat

 
T4 ____MKFTVVAAALL
C14 AAALLLLCAVRAEEE
T37 VVGIDLGTTYSCVGV
Y39 GIDLGTTYSCVGVFK
K81 RLIGDAAKNQLTSNP
K81 RLIGDAAKNQLTSNP
K96 ENTVFDAKRLIGRTW
K96 ENTVFDAKRLIGRTW
K113 PSVQQDIKFLPFKVV
K113 PSVQQDIKFLPFKVV
K118 DIKFLPFKVVEKKTK
K118 DIKFLPFKVVEKKTK
K122 LPFKVVEKKTKPYIQ
K123 PFKVVEKKTKPYIQV
K123 PFKVVEKKTKPYIQV
T124 FKVVEKKTKPYIQVD
K125 KVVEKKTKPYIQVDI
K125 KVVEKKTKPYIQVDI
Y127 VEKKTKPYIQVDIGG
K138 DIGGGQTKTFAPEEI
K138 DIGGGQTKTFAPEEI
S146 TFAPEEISAMVLTKM
K152 ISAMVLTKMKETAEA
K152 ISAMVLTKMKETAEA
K154 AMVLTKMKETAEAyL
T156 VLTKMKETAEAyLGK
Y160-p MKETAEAyLGKKVTH
K163 TAEAyLGKKVTHAVV
K163 TAEAyLGKKVTHAVV
K164 AEAyLGKKVTHAVVT
K185 DAQRQATKDAGTIAG
T189 QATKDAGTIAGLNVM
T203 MRIINEPTAAAIAYG
K213 AIAYGLDKREGEKNI
T229 VFDLGGGTFDVSLLT
K268 RVMEHFIKLYKKKTG
K268 RVMEHFIKLYKKKTG
K326 SETLTRAKFEELNMD
K326 SETLTRAKFEELNMD
K340 DLFRSTMKPVQKVLE
K340 DLFRSTMKPVQKVLE
K352 VLEDSDLKKSDIDEI
K352 VLEDSDLKKSDIDEI
K353 LEDSDLKKSDIDEIV
K353 LEDSDLKKSDIDEIV
S354 EDSDLKKSDIDEIVL
K370 GGSTRIPKIQQLVKE
K376 PKIQQLVKEFFNGKE
K376 PKIQQLVKEFFNGKE
T441 TKLIPRNTVVPTKKS
K447 NTVVPTKKSQIFSTA
K464 NQPTVTIKVYEGERP
Y466 PTVTIKVYEGERPLT
K474 EGERPLTKDNHLLGT
T518 VTAEDKGTGNKNKIT
K523 KGTGNKNKITITNDQ
K547 RMVNDAEKFAEEDKK
K547 RMVNDAEKFAEEDKK
Y570 NELESYAYSLKNQIG
K573 ESYAYSLKNQIGDKE
K579 LKNQIGDKEKLGGKL
K581 NQIGDKEKLGGKLSP
K585 DKEKLGGKLSPEDKE
K585 DKEKLGGKLSPEDKE
K585 DKEKLGGKLSPEDKE
K585 DKEKLGGKLSPEDKE
S587 EKLGGKLSPEDKETM
P588 KLGGKLSPEDKETME
K591 GKLSPEDKETMEKAV
K601 MEKAVEEKIEWLESH
K601 MEKAVEEKIEWLESH
K617 DADIEDFKAKKKELE
K619 DIEDFKAKKKELEEI
K633 IVQPIISKLYGSGGP
T643 GSGGPPPTGEEDtSE
T648-p PPTGEEDtSEKDEL_
S649 PTGEEDtSEKDEL__
K651 GEEDtSEKDEL____
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