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Protein Page:
BRCA1 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
BRCA1 the BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACC1 and preventing its dephosphorylation. Defects in BRCA1 are a cause of genetic susceptibility to breast cancer. Mutations in BRCA1 are thought to be responsible for more than 80% of inherited breast-ovarian cancer. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBN protein complex. This association may be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts (via BRCT domains) with CTIP. Associates with RNA polymerase II holoenzyme. Interacts with SMC1 and COBRA1. Interacts (via BRCT domains) with BRIP1. Interacts with FANCD2 (ubiquitinated). Interacts with BAP1. Interacts with Artemis and claspin. Interacts with H2AFX (phosphorylated on S140). Interacts with CHK1. Interacts with BRCC3. Five human isoforms are produced by alternative splicing and alternative initiation. Isoform 1 and isoform 3 are widely expressed. Isoform 1 is largely nuclear, while isoforms 3 and 5 are cytoplasmic. Note: This description may include information from UniProtKB.
Protein type: Ubiquitin ligase; Transcription, coactivator/corepressor; Tumor suppressor; EC 6.3.2.-; Nuclear receptor co-regulator; Ubiquitin conjugating system
Cellular Component: nucleoplasm; filamentous actin; ruffle; gamma-tubulin ring complex; protein complex; focal adhesion; BRCA1-BARD1 complex; plasma membrane; chromosome; ribonucleoprotein complex; nucleus; ubiquitin ligase complex
Molecular Function: tubulin binding; protein binding; enzyme binding; DNA binding; androgen receptor binding; zinc ion binding; RNA binding; ubiquitin protein ligase binding; transcription coactivator activity; ubiquitin-protein ligase activity
Biological Process: apoptosis; positive regulation of transcription, DNA-dependent; protein ubiquitination; positive regulation of histone H3-K4 methylation; chromosome segregation; regulation of apoptosis; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; double-strand break repair; negative regulation of fatty acid biosynthetic process; fatty acid biosynthetic process; negative regulation of histone H3-K9 methylation; protein autoubiquitination; positive regulation of DNA repair; postreplication repair; DNA repair; negative regulation of centriole replication; double-strand break repair via homologous recombination; regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; DNA damage response, signal transduction resulting in induction of apoptosis; response to estrogen stimulus; regulation of transcription from RNA polymerase III promoter; positive regulation of protein ubiquitination; androgen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of histone acetylation; response to ionizing radiation; negative regulation of transcription, DNA-dependent; G2/M transition DNA damage checkpoint; response to DNA damage stimulus
Reference #:  P38398 (UniProtKB)
Alt. Names/Synonyms: BRCA1; BRCA1/BRCA2-containing complex, subunit 1; BRCAI; BRCC1; breast and ovarian cancer susceptibility protein 1; breast and ovarian cancer sususceptibility protein; breast cancer 1, early onset; Breast cancer type 1 susceptibility protein; BROVCA1; IRIS; PSCP; RING finger protein 53; RNF53
Gene Symbols: BRCA1
Molecular weight: 207,721 Da
Basal Isoelectric point: 5.29  Predict pI for various phosphorylation states
CST Pathways:  G2/M DNA Damage Checkpoint  |  Protein Acetylation
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

BRCA1

Protein Structure Not Found.


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Sites Implicated In
cell cycle regulation: S1387‑p, S1423‑p, S1524‑p
transcription, altered: S632‑p
transcription, induced: T509‑p
activity, induced: S988‑p
intracellular localization: T509‑p, S988‑p, S1143‑p, S1189‑p, S1191‑p, S1280‑p, S1497‑p
molecular association, regulation: S1189‑p, S1423‑p, S1457‑p, S1497‑p, S1524‑p, S1542‑p
phosphorylation: S1423‑p, S1524‑p
protein stabilization: S694‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 5 S114-p FAKKENNsPEHLkDE
1 0 K119-s NNsPEHLkDEVSIIQ
0 1 S184 SVYIELGSDSSEDTV
0 1 S186 YIELGSDSSEDTVNK
0 1 S187 IELGSDSSEDTVNKA
0 6 T208-p DQELLQItPQGTRDE
1 0 S308-p KAEFCNKsKQPGLAR
0 5 S316 KQPGLARSQHNRWAG
0 5 S395-p RSDELLGsDDsHDGE
0 4 S398-p ELLGsDDsHDGESES
0 1 S423-p LNEVDEYsGSSEKID
0 2 S434-p EKIDLLAsDPHEALI
0 1 S451-p SERVHSKsVEsNIED
0 1 S454-p VHSKsVEsNIEDKIF
3 75 T509-p LKRKRRPtsGLHPED
0 7 S510-p KRKRRPtsGLHPEDF
0 1 G511 RKRRPtsGLHPEDFI
0 1 S551-p QVMNITNsGHENKTK
0 36 S615-p KNRLRRKsstRHIHA
0 36 S616-p NRLRRKsstRHIHAL
0 5 T617-p RLRRKsstRHIHALE
1 1 S632-p LVVSRNLsPPNCTEL
0 1 S643 CTELQIDSCSSSEEI
0 1 - gap
1 9 S694-p QTSKRHDsDTFPELK
0 1 S708-p KLTNAPGsFTKCSNT
0 1 S713 PGsFTKCSNTSELKE
0 4 N714 GsFTKCSNTSELKEF
0 2 L718 KCSNTSELKEFVNPS
0 3 S725 LKEFVNPSLPREEKE
0 5 S753-p DPKDLMLsGERVLQT
0 6 S803-p TEPNKCVsQCAAFEN
0 2 S840 LGHEVNHSRETSIEM
0 1 S868 FKVSKRQSFAPFSNP
0 1 T967-p GNETGLItPNKHGLL
5 0 S988-p PPLFPIKsFVKTKCK
0 1 S1009-p NFEEHSMsPEREMGN
1 0 S1143-p PMGSSHAsQVCSETP
0 6 S1174-p ENDIKESsAVFSKSV
0 5 S1180 SsAVFSKSVQKGELs
0 7 S1187-p SVQKGELsRsPsPFT
4 11 S1189-p QKGELsRsPsPFTHT
2 7 S1191-p GELsRsPsPFTHTHL
0 4 S1211-p RGAKKLEssEENLss
0 3 S1212-p GAKKLEssEENLssE
0 7 S1217-p EssEENLssEDEELP
0 7 S1218-p ssEENLssEDEELPC
0 1 - gap
0 2 - gap
0 10 S1239-p GKVNNIPsQSTRHsT
0 2 S1245-p PsQSTRHsTVATECL
1 1 S1280-p QVILAKAsQEHHLsE
0 1 S1286-p AsQEHHLsEETKCSA
0 3 S1328-p SKQMRHQsEsQGVGL
0 3 S1330-p QMRHQsEsQGVGLsD
0 10 S1336-p EsQGVGLsDKELVsD
0 5 S1342-p LsDKELVsDDEERGT
6 0 S1387-p EDCSGLSsQSDILTT
11 2 S1423-p AVLEQHGsQPSNSYP
4 3 S1457-p SEKAVLTsQKsSEYP
0 3 S1460-p AVLTsQKsSEYPIsQ
0 4 S1466-p KsSEYPIsQNPEGLS
0 1 S1483-p KFEVSADsSTSKNKE
5 5 S1497-p EPGVERSsPSKCPSL
0 2 - gap
8 8 S1524-p LQNRNYPsQEELIKV
1 3 S1542-p EEQQLEEsGPHDLTE
0 1 T1550-p GPHDLTEtSYLPRQD
1 0 S1572-p ESGISLFsDDPEsDP
0 1 S1577-p LFsDDPEsDPSEDRA
0 1 - gap
0 1 - gap
0 1 S1598 GNIPSSTSALKVPQL
0 2 S1613-p KVAESAQsPAAAHTT
0 1 K1667-u EEFMLVYkFARKHHI
0 1 T1700-p AEFVCERtLKYFLGI
0 1 T1720-p VVSYFWVtQSIKERK
0 1 S1722 SYFWVtQSIKERKML
9009 : Phospho-BRCA1 (Ser1524) Antibody
  BRCA1 iso6  
S114 FAKKENNSPEHLKDE
K119 NNSPEHLKDEVSIIQ
S184 SVYIELGSDSSEDTV
S186 YIELGSDSSEDTVNK
S187 IELGSDSSEDTVNKA
T208 DQELLQITPQGTRDE
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S284 EDCSGLSSQSDILTT
S320 AVLEQHGSQPSNSYP
S353 TSEKVLTSQKsSEYP
S356-p KVLTSQKsSEYPIsQ
S362-p KsSEYPIsQNPEGLS
S379 KFEVSADSSTSKNKE
S393 EPGVERSSPSKCPSL
- gap
S420 LQNRNYPSQEELIKV
S438 EEQQLEESGPHDLTE
T446 GPHDLTETSYLPRQD
S468 ESGISLFSDDPESDP
S473 LFSDDPESDPSEDRA
- gap
- gap
S494 GNIPSSTSALKVPQL
S509 KVAESAQSPAAAHTT
K563 EEFMLVYKFARKHHI
T596 AEFVCERTLKYFLGI
T616 VVSYFWVTQSIKERK
S618 SYFWVTQSIKERKML
  mouse

 
S114-p FSKKRNNsCERLNEE
- gap
S183-p SVYIELDsDssEETV
S185-p YIELDsDssEETVTK
S186-p IELDsDssEETVTKP
- gap
S305 KAEFCNKSKQPGIAV
S313-p KQPGIAVsQQSRWAA
S392 RTGEMLTSDSASARR
- gap
- gap
- gap
- gap
- gap
S502-p NKLKRKRstsLQPED
T503-p KLKRKRstsLQPEDF
S504-p LKRKRstsLQPEDFI
- gap
S609 KNRLRRKSSIRCALP
S610 NRLRRKSSIRCALPL
- gap
S625-p EPISRNPsPPTCAEL
S636-p CAELQIDsCGSSEEt
T643-p sCGSSEEtKKNHSNQ
S686-p HIRKRRAsDAFPEEK
- gap
S705-p AGLLTSCssPRKsQG
S706-p GLLTSCssPRKsQGP
S710-p SCssPRKsQGPVNPs
S717-p sQGPVNPsPQRTGTE
- gap
T795 TGSAQCMTQFVASEN
S831-p LRHALNLsQEKVEME
S858-p FQVSKRQsFALFSKP
- gap
S971-p QSVSPIRsSIKTDNR
S992 RFERHTSSTEMAVGN
- gap
S1137-p EGDIMERsAVFNGsI
S1143-p RsAVFNGsILRRESs
S1150-p sILRRESsRsPsPVT
S1152-p LRRESsRsPsPVTHA
S1154-p RESsRsPsPVTHASK
S1174 RASRKLESSEESDST
S1175 ASRKLESSEESDSTE
S1180 ESSEESDSTEDEDLP
T1181 SSEESDSTEDEDLPC
S1197-p QHLLSRIsNtPELTR
T1199-p LLSRIsNtPELTRCS
- gap
S1206 tPELTRCSSAVTQRM
S1241 EVIMIEASQEHQFSE
S1247 ASQEHQFSEDPRCSG
- gap
- gap
S1297-p GNEEAFLsDKELISD
S1303 LsDKELISDNEEMAT
S1343 TNLSEDCSQSDILTT
- gap
S1413 SGAAILTSKNINENP
- gap
S1422-p NINENPVsQNLKSAC
- gap
S1454-p ESGMGRPsPFKsPLA
S1458-p GRPsPFKsPLAGSRG
S1481 LQKRNSPSQEELLQP
S1495 PAGSEASSEPHNSTG
- gap
S1525 GSGISLFSSRDPESE
R1527 GISLFSSRDPESESP
T1542-p KEPAHIGttPASTsA
T1543-p EPAHIGttPASTsAL
S1548-p GttPASTsALKIPQG
S1561 QGQVAFRSAAAAGAD
K1610 KEVMTVQKFAEKYRL
T1643 AEFVCERTLKYFLGI
- gap
S1665-p SYSWVVRsIQERRLL
  rat

 
S114 FSKKKNSSSELLNED
- gap
S183 SVYIALESDSSEERV
S185 YIALESDSSEERVNA
S186 IALESDSSEERVNAP
- gap
S306 KAEFCDRSKQSGAAV
S314 KQSGAAVSQQSRWAD
S393 RTGEMLTSDNASDRR
- gap
- gap
- gap
- gap
- gap
S503 NKLKRKRSTCLHPED
T504 KLKRKRSTCLHPEDF
- gap
- gap
S610 KNRLRRKSTRCVLPL
T611 NRLRRKSTRCVLPLE
- gap
S625 EPISRNPSPPTCAEL
S636 CAELQIESCGSSEET
T643 SCGSSEETKKNNSNQ
S686 HIRKRSASDAFPEEK
- gap
S705 AGLLTSCSSPRKPQG
S706 GLLTSCSSPRKPQGP
- gap
S717 PQGPVNPSPERKGIE
- gap
T795 TGSVQCMTQFVASEN
N831 LRHELNHNQETIEME
S858 FQVSKRQSFALFSKL
T954 SCENGLNTTDKSGIS
S975 QSVSPLRSSIKTDNR
- gap
- gap
S1138 EGGITEKSAIFNGSV
S1144 KSAIFNGSVLRRESS
S1151 SVLRRESSRSPSPVT
S1153 LRRESSRSPSPVTHA
S1155 RESSRSPSPVTHASK
- gap
S1176 GSRKLEFSEESDSTE
S1181 EFSEESDSTEDEDLP
T1182 FSEESDSTEDEDLPC
S1198 QHLLSRVSSTPELTR
T1200 LLSRVSSTPELTRCS
- gap
S1207 TPELTRCSSVVTQRV
S1242 EVILGEASQEYQFSE
S1248 ASQEYQFSEDAKCSG
- gap
- gap
S1298 ENEEAFLSDKELISD
S1304 LSDKELISDHEDMAA
S1344 ANLSEDCSQSDILTT
S1380 AVLEQHGSQPSGHPP
S1414 SGTAILTSKNINENP
- gap
S1423 NINENPVSQNPKRAC
- gap
S1455 ESGMRRPSPFKSPLT
S1459 RRPSPFKSPLTSSRC
S1482 LQNRNSTSQEELLQP
- gap
- gap
S1526 ESGIRLVSSRDPDSE
R1528 GIRLVSSRDPDSESP
T1543 KVSALVCTAPASTSA
A1544 VSALVCTAPASTSAL
S1549 CTAPASTSALKISQG
S1564 QVAGSCRSPAAGGAD
K1613 KEVMIVQKFAEKYRL
T1646 AEFVCERTLKYFLGI
- gap
S1668 SYSWVIKSIQERKLL
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