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Protein Page:
BAD (mouse)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
BAD a proapoptotic member of the Bcl-2 family. Displaces Bax from binding to Bcl-2 and Bcl-xL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad inducing the phosphorylation of Bad by Akt and p90RSK. 14-3-3 proteins bind phosphorylated Bad, inhibiting its binding to Bcl-2 and Bcl-xL. Phosphorylation by mitochondria-anchored PKA in the BH3 domain can block the dimerization of Bad and Bcl-xL. Note: This description may include information from UniProtKB.
Protein type: Apoptosis
Cellular Component: mitochondrial outer membrane; mitochondrion; membrane; cytoplasm; intracellular; cytosol
Molecular Function: protein kinase B binding; protein binding; protein heterodimerization activity; phospholipid binding; caspase activator activity; protein phosphatase binding; protein kinase binding; lipid binding; protein phosphatase 2B binding
Biological Process: positive regulation of apoptosis; apoptosis; positive regulation of proteolysis; positive regulation of caspase activity; regulation of caspase activity; cellular process regulating host cell cycle in response to virus; glucose homeostasis; positive regulation of apoptosis by virus; regulation of apoptosis; pore complex biogenesis; positive regulation of glucokinase activity; caspase activation; release of cytochrome c from mitochondria; cytokine and chemokine mediated signaling pathway; suppression by virus of host apoptosis; ADP metabolic process; positive regulation of insulin secretion; ATP metabolic process; cell proliferation; glucose catabolic process; DNA damage response, signal transduction resulting in induction of apoptosis; induction of apoptosis via death domain receptors; positive regulation of T cell differentiation; positive regulation of B cell differentiation; regulation of mitochondrial membrane permeability; positive regulation of epithelial cell proliferation
Reference #:  Q61337 (UniProtKB)
Alt. Names/Synonyms: AI325008; BAD; Bbc2; Bbc6; Bcl-2-binding component 6; Bcl-associated death promoter; Bcl-xL/Bcl-2-associated death promoter; Bcl2 antagonist of cell death; BCL2-associated agonist of cell death; OTTMUSP00000017561
Gene Symbols: Bad
Molecular weight: 22,080 Da
Basal Isoelectric point: 9.15  Predict pI for various phosphorylation states
CST Pathways:  Apoptosis Regulation  |  ErbB/HER Signaling  |  Growth And Differentiation Control by MAPKs  |  Inhibition of Apoptosis  |  Insulin Receptor Signaling  |  Mitochondrial Control of Apoptosis  |  PI3K/Akt Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

BAD

Protein Structure Not Found.


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Sites Implicated In
apoptosis, altered: S112‑p, S136‑p, S155‑p
apoptosis, induced: S128‑p
apoptosis, inhibited: S112‑p, S136‑p, S155‑p, S170‑p, T201‑p
cell cycle regulation: S170‑p
cell growth, altered: S136‑p
cell growth, inhibited: S170‑p
activity, induced: S112‑p, S136‑p, T201‑p
activity, inhibited: S136‑p
intracellular localization: S112‑p, S136‑p
molecular association, regulation: S112‑p, S128‑p, S136‑p, S155‑p, T201‑p
phosphorylation: S136‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       mouse

 
1 0 S26-p KSDPGIRsLGSDAGG
0 7 S67 TDRGLGPSLTEDQPG
0 2 G74 SLTEDQPGPyLAPGL
0 5 Y76-p TEDQPGPyLAPGLLG
0 1 - gap
1 0 T94-p HQQGRAAtNsHHGGA
1 0 S96-p QGRAAtNsHHGGAGA
0 29 S108-p AGAMETRsRHssyPA
1 76 S111-p METRsRHssyPAGtE
113 179 S112-p ETRsRHssyPAGtEE
0 49 Y113-p TRsRHssyPAGtEED
1 28 T117-p HssyPAGtEEDEGME
7 44 S128-p EGMEEELsPFRGRsR
1 20 S134-p LsPFRGRsRsAPPNL
111 240 S136-p PFRGRsRsAPPNLWA
0 1 Y147 NLWAAQRYGRELRRM
43 195 S155-p GRELRRMsDEFEGSF
1 1 S161 MsDEFEGSFKGLPRP
6 13 S170-p KGLPRPKsAGtATQM
2 0 T173-p PRPKsAGtATQMRQS
0 1 T175 PKsAGtATQMRQSAG
0 1 G182 TQMRQSAGWTRIIQS
0 5 K197 WWDRNLGKGGStPSQ
3 0 T201-p NLGKGGStPSQ____
5193 : Phospho-Bad (Ser112) (7E11) Mouse mAb (Biotinylated)
5284 : Phospho-Bad (Ser112) (40A9) Rabbit mAb
9291 : Phospho-Bad (Ser112) Antibody
9296 : Phospho-Bad (Ser112) (7E11) Mouse mAb
11865 : Phospho-Bad (Ser112) (40A9) Rabbit mAb (PE Conjugate)
4366 : Phospho-Bad (Ser136) (D25H8) Rabbit mAb
5286 : Phospho-Bad (Ser136) (185D10) Rabbit mAb
9295 : Phospho-Bad (Ser136) Antibody
9297 : Phospho-Bad (Ser155) Antibody
  human

 
- gap
S25-p AERGLGPsPAGDGPs
S32-p sPAGDGPsGsGkHHR
S34-p AGDGPsGsGkHHRQA
K36-ac DGPsGsGkHHRQAPG
S57 HQQEQPTSSSHHGGA
S59 QEQPTSSSHHGGAGA
S71-p AGAVEIRsRHssyPA
S74-p VEIRsRHssyPAGtE
S75-p EIRsRHssyPAGtED
Y76-p IRsRHssyPAGtEDD
T80-p HssyPAGtEDDEGMG
S91-p EGMGEEPsPFRGRsR
S97-p PsPFRGRsRsAPPNL
S99-p PFRGRsRsAPPNLWA
Y110-p NLWAAQRyGRELRRM
S118-p GRELRRMsDEFVDsF
S124-p MsDEFVDsFKKGLPR
S134-p KGLPRPKsAGtAtQM
T137-p PRPKsAGtAtQMRQS
T139-p PKsAGtAtQMRQSSs
S146-p tQMRQSSsWTRVFQS
R161-m1 WWDRNLGrGSSAPSQ
A165 NLGrGSSAPSQ____
5193 : Phospho-Bad (Ser112) (7E11) Mouse mAb (Biotinylated)
5284 : Phospho-Bad (Ser112) (40A9) Rabbit mAb
9291 : Phospho-Bad (Ser112) Antibody
9296 : Phospho-Bad (Ser112) (7E11) Mouse mAb
11865 : Phospho-Bad (Ser112) (40A9) Rabbit mAb (PE Conjugate)
4366 : Phospho-Bad (Ser136) (D25H8) Rabbit mAb
5286 : Phospho-Bad (Ser136) (185D10) Rabbit mAb
9295 : Phospho-Bad (Ser136) Antibody
9297 : Phospho-Bad (Ser155) Antibody
  rat

 
S26 KSDPGIRSLGSDAGG
S67 TDRGLGPSLTEDQPG
G74 SLTEDQPGPYLAPGL
Y76 TEDQPGPYLAPGLLG
- gap
N95 QQPGQAANNSHHGGA
S97 PGQAANNSHHGGAGT
S109 AGTMETRSRHSsYPA
S112 METRSRHSsYPAGtE
S113-p ETRSRHSsYPAGtEE
Y114 TRSRHSsYPAGtEED
T118-p HSsYPAGtEEDEGME
S129-p EGMEEELsPFRGRsR
S135-p LsPFRGRsRsAPPNL
S137-p PFRGRsRsAPPNLWA
Y148 NLWAAQRYGRELRRM
S156-p GRELRRMsDEFEGSF
S162 MsDEFEGSFKGLPRP
S171 KGLPRPKSAGTATQM
T174 PRPKSAGTATQMRQS
T176 PKSAGTATQMRQSAs
S183-p TQMRQSAsWTRIIQS
K198 WWDRNLGKGGSTPSQ
T202 NLGKGGSTPSQ____
5193 : Phospho-Bad (Ser112) (7E11) Mouse mAb (Biotinylated)
5284 : Phospho-Bad (Ser112) (40A9) Rabbit mAb
9291 : Phospho-Bad (Ser112) Antibody
9296 : Phospho-Bad (Ser112) (7E11) Mouse mAb
11865 : Phospho-Bad (Ser112) (40A9) Rabbit mAb (PE Conjugate)
4366 : Phospho-Bad (Ser136) (D25H8) Rabbit mAb
5286 : Phospho-Bad (Ser136) (185D10) Rabbit mAb
9295 : Phospho-Bad (Ser136) Antibody
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