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Protein Page:
UBE2N (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
UBE2N The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Heterodimer with UBE2V2. Interacts (UBE2V2-UBE2N heterodimer) with the E3 ligase STUB1 (via the U-box domain); the complex has a specific 'Lys-63'-linked polyubiquitination activity. Interacts with RNF8 and RNF168. Interacts with RNF11. Interacts with the E3 ligases, HLTF and SHPRH; the interactions promote the 'Lys-63'-linked polyubiquitination of PCNA upon genotoxic stress and lead to DNA repair. Interacts with ARIH2 (via RING-type 2). Belongs to the ubiquitin-conjugating enzyme family. Note: This description may include information from UniProtKB.
Protein type: EC 6.3.2.19; Ubiquitin conjugating system; DNA repair, damage; Ubiquitin ligase; Ligase
Cellular Component: cytoplasm; nucleus; cytosol; ubiquitin ligase complex; UBC13-MMS2 complex
Molecular Function: protein binding; ubiquitin binding; ubiquitin-protein ligase activity; ATP binding
Biological Process: ubiquitin-dependent protein catabolic process; regulation of histone ubiquitination; positive regulation of DNA repair; positive regulation of I-kappaB kinase/NF-kappaB cascade; postreplication repair; positive regulation of histone modification; cytokine and chemokine mediated signaling pathway; DNA double-strand break processing; protein modification process; protein ubiquitination; proteolysis; T cell receptor signaling pathway; double-strand break repair via homologous recombination; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 2 signaling pathway; toll-like receptor 5 signaling pathway; regulation of DNA repair; MyD88-dependent toll-like receptor signaling pathway; positive regulation of ubiquitin-protein ligase activity; histone ubiquitination; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway
Reference #:  P61088 (UniProtKB)
Alt. Names/Synonyms: bendless-like ubiquitin conjugating enzyme; Bendless-like ubiquitin-conjugating enzyme; BLU; MGC131857; MGC8489; Ubc13; UbcH-ben; UBE2N; Ubiquitin carrier protein N; Ubiquitin-conjugating enzyme E2 N; ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13); ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast); Ubiquitin-protein ligase N; yeast UBC13 homolog
Gene Symbols: UBE2N
Molecular weight: 17,138 Da
Basal Isoelectric point: 6.13  Predict pI for various phosphorylation states
CST Pathways:  NF-kB Signaling  |  Toll-Like Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

UBE2N

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
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Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

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 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 15 K10-u GLPRRIIkETQRLLA
0 20 K24-u AEPVPGIkAEPDESN
0 8 K68-u EYPMAAPkVRFMTkI
0 40 K74-u PkVRFMTkIYHPNVD
0 3 K82-a IYHPNVDkLGRICLD
0 142 K82-u IYHPNVDkLGRICLD
0 8 K92-a RICLDILkDkWSPAL
0 161 K92-u RICLDILkDkWSPAL
0 3 K94-a CLDILkDkWSPALQI
0 131 K94-u CLDILkDkWSPALQI
  mouse

 
K10-u GLPRRIIkETQRLLA
K24-u AEPVPGIkAEPDESN
K68-u EYPMAAPkVRFMTkI
K74-u PkVRFMTkIYHPNVD
K82 IYHPNVDKLGRICLD
K82-u IYHPNVDkLGRICLD
K92 RICLDILKDkWSPAL
K92-u RICLDILkDkWSPAL
K94 CLDILkDKWSPALQI
K94-u CLDILkDkWSPALQI
  rat

 
K10 GLPRRIIKETQRLLA
K24-u AEPVPGIkAEPDESN
K68 EYPMAAPKVRFMTkI
K74-u PKVRFMTkIYHPNVD
K82 IYHPNVDKLGRICLD
K82-u IYHPNVDkLGRICLD
K92 RICLDILKDkWSPAL
K92-u RICLDILkDkWSPAL
K94 CLDILkDKWSPALQI
K94-u CLDILkDkWSPALQI
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