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Protein Page:
UBE2N (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
UBE2N The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Heterodimer with UBE2V2. Interacts (UBE2V2-UBE2N heterodimer) with the E3 ligase STUB1 (via the U-box domain); the complex has a specific 'Lys-63'-linked polyubiquitination activity. Interacts with RNF8 and RNF168. Interacts with RNF11. Interacts with the E3 ligases, HLTF and SHPRH; the interactions promote the 'Lys-63'-linked polyubiquitination of PCNA upon genotoxic stress and lead to DNA repair. Interacts with ARIH2 (via RING-type 2). Belongs to the ubiquitin-conjugating enzyme family. Note: This description may include information from UniProtKB.
Protein type: Ligase; Ubiquitin conjugating system; EC 6.3.2.19; Ubiquitin ligase; DNA repair, damage
Chromosomal Location of Human Ortholog: 12q22
Cellular Component: protein complex; cytoplasm; nucleus; cytosol; ubiquitin ligase complex; UBC13-MMS2 complex
Molecular Function: protein binding; ubiquitin binding; acid-amino acid ligase activity; ubiquitin-protein ligase activity; ATP binding
Biological Process: ubiquitin-dependent protein catabolic process; regulation of histone ubiquitination; positive regulation of DNA repair; positive regulation of I-kappaB kinase/NF-kappaB cascade; postreplication repair; positive regulation of histone modification; cytokine and chemokine mediated signaling pathway; DNA double-strand break processing; protein ubiquitination; protein modification process; proteolysis; T cell receptor signaling pathway; double-strand break repair via homologous recombination; activation of NF-kappaB transcription factor; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; regulation of DNA repair; positive regulation of ubiquitin-protein ligase activity; histone ubiquitination; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway
Reference #:  P61088 (UniProtKB)
Alt. Names/Synonyms: bendless-like ubiquitin conjugating enzyme; Bendless-like ubiquitin-conjugating enzyme; BLU; MGC131857; MGC8489; Ubc13; UbcH-ben; UBE2N; Ubiquitin carrier protein N; Ubiquitin-conjugating enzyme E2 N; ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13); ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast); Ubiquitin-protein ligase N; yeast UBC13 homolog
Gene Symbols: UBE2N
Molecular weight: 17,138 Da
Basal Isoelectric point: 6.13  Predict pI for various phosphorylation states
CST Pathways:  NF-kB Signaling  |  Toll-Like Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

UBE2N

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 K10-ac GLPRRIIkETQRLLA
0 15 K10-ub GLPRRIIkETQRLLA
0 20 K24-ub AEPVPGIkAEPDESN
0 1 Y34-p PDESNARyFHVVIAG
0 1 K53-ac PFEGGTFkLELFLPE
0 8 K68-ub EYPMAAPkVRFMTkI
0 40 K74-ub PkVRFMTkIYHPNVD
0 4 K82-ac IYHPNVDkLGRICLD
0 142 K82-ub IYHPNVDkLGRICLD
0 9 K92-ac RICLDILkDkWSPAL
0 161 K92-ub RICLDILkDkWSPAL
0 1 K92-sc RICLDILkDkWSPAL
0 3 K94-ac CLDILkDkWSPALQI
0 131 K94-ub CLDILkDkWSPALQI
0 1 T139-p NEAQAIEtARAWtRL
0 1 T144-p IEtARAWtRLyAMNN
0 1 Y147-p ARAWtRLyAMNNI__
  mouse

 
K10 GLPRRIIKETQRLLA
K10-ub GLPRRIIkETQRLLA
K24-ub AEPVPGIkAEPDESN
Y34 PDESNARYFHVVIAG
K53 PFEGGTFKLELFLPE
K68-ub EYPMAAPkVRFMTkI
K74-ub PkVRFMTkIYHPNVD
K82 IYHPNVDKLGRICLD
K82-ub IYHPNVDkLGRICLD
K92 RICLDILKDkWSPAL
K92-ub RICLDILkDkWSPAL
K92 RICLDILKDkWSPAL
K94 CLDILkDKWSPALQI
K94-ub CLDILkDkWSPALQI
T139 NEAQAIETARAWTRL
T144 IETARAWTRLYAMNN
Y147 ARAWTRLYAMNNI__
  rat

 
K10-ac GLPRRIIkETQRLLA
K10 GLPRRIIKETQRLLA
K24-ub AEPVPGIkAEPDESN
Y34 PDESNARYFHVVIAG
K53 PFEGGTFKLELFLPE
K68 EYPMAAPKVRFMTkI
K74-ub PKVRFMTkIYHPNVD
K82-ac IYHPNVDkLGRICLD
K82-ub IYHPNVDkLGRICLD
K92 RICLDILKDkWSPAL
K92-ub RICLDILkDkWSPAL
K92 RICLDILKDkWSPAL
K94-ac CLDILkDkWSPALQI
K94-ub CLDILkDkWSPALQI
T139 NEAQAIETARAWTRL
T144 IETARAWTRLYAMNN
Y147 ARAWTRLYAMNNI__
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