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Protein Page:
PARP1 (mouse)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PARP1 Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP- ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423. Interacts (when poly-ADP- ribosylated) with CHD1L. Interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression. Interacts with EEF1A1, RNF4 and TXK. Note: This description may include information from UniProtKB.
Protein type: Nucleolus; EC 2.4.2.30; Transferase; Nuclear envelope; Nuclear receptor co-regulator; DNA repair, damage
Cellular Component: nucleoplasm; transcription factor complex; protein complex; membrane; cytoplasm; nucleolus; nuclear envelope; nucleus
Molecular Function: transferase activity; identical protein binding; protein binding; transferase activity, transferring glycosyl groups; enzyme binding; DNA binding; zinc ion binding; metal ion binding; protein N-terminus binding; NAD binding; transcription factor binding; NAD+ ADP-ribosyltransferase activity
Biological Process: transcription, DNA-dependent; DNA damage response, detection of DNA damage; DNA repair; protein autoprocessing; protein amino acid ADP-ribosylation; regulation of transcription, DNA-dependent; cellular response to insulin stimulus; base-excision repair; double-strand break repair; transforming growth factor beta receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; regulation of growth rate; telomere maintenance; response to DNA damage stimulus; DNA metabolic process
Reference #:  P11103 (UniProtKB)
Alt. Names/Synonyms: 5830444G22Rik; ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase) 1; Adprp; Adprt; ADPRT 1; Adprt1; AI893648; C80510; msPARP; NAD(+) ADP-ribosyltransferase 1; PARP; PARP-1; Parp1; poly (ADP-ribose) polymerase family, member 1; poly (ADP-ribose) polymerase) 1; Poly [ADP-ribose] polymerase 1; Poly[ADP-ribose] synthase 1; poly[ADP-ribose] synthetase 1; PPOL; sPARP-1
Gene Symbols: Parp1
Molecular weight: 113,100 Da
Basal Isoelectric point: 9.05  Predict pI for various phosphorylation states
CST Pathways:  Death Receptor Signaling  |  NF-kB Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PARP1

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       mouse

► Hide Isoforms
 
0 1 S5 ___MAEASERLYRVQ
0 1 S25 RASCKKCSESIPKDS
0 1 S27 SCKKCSESIPKDSLR
0 1 S32 SESIPKDSLRMAIMV
0 4 S41 RMAIMVQSPMFDGKV
0 1 K87 DDQQKVKKtAEAGGV
0 2 T88-p DQQKVKKtAEAGGVA
0 3 K97 EAGGVAGKGQDGSGG
0 1 K97 EAGGVAGKGQDGSGG
0 3 K105-ac GQDGSGGkAEKTLGD
0 1 K108 GSGGkAEKTLGDFAA
0 1 K108 GSGGkAEKTLGDFAA
0 8 K119 DFAAEYAKSNRSMCK
0 1 K131 MCKGCLEKIEKGQMR
0 1 K148 KKMVDPEKPQLGMID
0 2 K165 YHPTCFVKKRDELGF
1 2 S177 LGFRPEYSASQLKGF
0 4 S179 FRPEYSASQLKGFSL
0 2 S185 ASQLKGFSLLSAEDK
0 1 A189 KGFSLLSAEDKEALK
1 1 K203 KKQLPAIKNEGKRKG
0 1 K209 IKNEGKRKGDEVDGT
0 1 K221 DGTDEVAKKKSRKET
0 1 S224 DEVAKKKSRKETDKY
0 1 K239 SKLEKALKAQNELIW
0 3 K249 NELIWNIKDELKKAC
0 1 K249 NELIWNIKDELKKAC
0 7 K253 WNIKDELKKACSTND
0 1 K254 NIKDELKKACSTNDL
0 1 K254 NIKDELKKACSTNDL
0 1 S257 DELKKACSTNDLKEL
0 3 K262 ACSTNDLKELLIFNQ
0 36 Q269 KELLIFNQQQVPSGE
0 3 S274 FNQQQVPSGESAILD
0 1 S277 QQVPSGESAILDRVA
0 1 K296-ub FGALLPCkECSGQLV
0 1 K320 GDVTAWTKCMVKTQN
0 1 K331 KTQNPSRKEWVTPKE
0 1 K337 RKEWVTPKEFREISY
0 1 S362 RIFPPESSAPITVHW
0 2 - gap
0 4 H368 SSAPITVHWPLSVTS
2 2 S372 ITVHWPLSVTSAPTA
2 1 - gap
0 1 S375 HWPLSVTSAPTAVNS
0 1 S391 APADKPLSNMKILTL
0 1 K394 DKPLSNMKILTLGKL
0 2 K400 MKILTLGKLSQNKDE
0 6 K400 MKILTLGKLSQNKDE
0 2 K414 EAKAVIEKLGGKLTG
0 2 K425 KLTGSANKASLCISI
0 3 K433 ASLCISIKKEVEKMN
0 1 K434 SLCISIKKEVEKMNK
0 1 K441 KEVEKMNKKMEEVKE
0 1 K442 EVEKMNKKMEEVKEA
0 7 K447 NKKMEEVKEANIRVV
1 0 S465 FLQDVSASTKSLQDL
0 3 K467 QDVSASTKSLQDLLS
0 1 K486 SPWGAEVKAEPGEVV
3 1 K486 SPWGAEVKAEPGEVV
1 0 K498 EVVAPRGKSAAPSKK
0 1 S499 VVAPRGKSAAPSKKS
1 0 K504 GKSAAPSKKSKGCFK
1 0 K507 AAPSKKSKGCFKEEG
1 6 K507 AAPSKKSKGCFKEEG
0 3 K517 FKEEGVNKSEKRMKL
1 26 K520 EGVNKSEKRMKLTLK
1 0 K523 NKSEKRMKLTLKGGA
0 2 K527 KRMKLTLKGGAAVDP
0 1 K547-ac HSAHVLEkGGKVFSA
0 13 K547-ub HSAHVLEkGGKVFSA
0 1 K550 HVLEkGGKVFSATLG
0 5 K563-ub LGLVDIVkGTNSYYK
0 2 K578-ub LQLLEDDkESRYWIF
0 1 K599 GTVIGSNKLEQMPSK
0 1 K599 GTVIGSNKLEQMPSK
0 1 S605 NKLEQMPSKEEAVEQ
0 1 Y617-p VEQFMKLyEEKTGNA
0 3 K620 FMKLyEEKTGNAWHS
0 1 K636 NFTKYPKKFYPLEID
0 1 T660 KLTVKPGTKSKLPKP
0 1 K661 LTVKPGTKSKLPKPV
0 1 K663 VKPGTKSKLPKPVQE
0 1 K666 GTKSKLPKPVQELVG
0 1 - gap
0 1 S680 GMIFDVDSMKKALVE
0 1 K694 EYEIDLQKMPLGKLS
0 1 S732 ESQILDLSNRFYTLI
0 5 K747 PHDFGMKKPPLLNNA
0 1 Y774 LLDIEVAYSLLRGGs
2 17 S781-p YSLLRGGsDDSSKDP
2 0 S784 LRGGsDDSSKDPIDV
1 1 S785 RGGsDDSSKDPIDVN
0 3 K786 GGsDDSSKDPIDVNY
0 5 K795 PIDVNYEKLKTDIKV
0 1 K797 DVNYEKLKTDIKVVD
0 1 S807 IKVVDRDSEEAEVIR
0 1 T823 YVKNTHATTHNAYDL
0 1 K837 LEVIDIFKIEREGES
0 3 R851 SQRYKPFRQLHNRRL
0 1 S873 TNFAGILSQGLRIAP
0 6 K939 KHASHISKLPKGKHS
0 7 K948 PKGKHSVKGLGKTTP
0 2 K1009 LKLKFNFKTSLW___
  PARP1 iso3  
S5 ___MAEASERLYRVE
S25 RASCKKCSESIPKDS
S27 SCKKCSESIPKDSLR
S32 SESIPKDSLRMTIMV
S41 RMTIMVQSPMFDGKV
K87 DDQQKVKKTAEAGGV
T88 DQQKVKKTAEAGGVA
K97 EAGGVAGKGQDGSGG
K97 EAGGVAGKGQDGSGG
K105 GQDGSGGKAEKTLGD
K108 GSGGKAEKTLGDFLA
K108 GSGGKAEKTLGDFLA
K119-ub DFLAEYAkSNRSMCK
K131 MCKGCLEKIEKGQMR
K148 KKMVDPEKPQLGMID
K165 YHPTCFVKKRDELGF
S177 LGFRPEYSASQLKGF
S179 FRPEYSASQLKGFSL
S185 ASQLKGFSLLSAEDK
A189 KGFSLLSAEDKEALK
K203 KKQLPAIKNEGKRKG
K209 IKNEGKRKGDEVDGT
K221 DGTDEVAKKKSKKGK
S224 DEVAKKKSKKGKDKD
K240 SKLEKALKAQNELIW
K250 NELIWNIKDELKKAC
K250 NELIWNIKDELKKAC
K254 WNIKDELKKACSTND
K255 NIKDELKKACSTNDL
K255 NIKDELKKACSTNDL
S258 DELKKACSTNDLKEL
K263 ACSTNDLKELLIFNQ
Q270 KELLIFNQQQVPSGE
S275 FNQQQVPSGESAILD
S278 QQVPSGESAILDRVA
K297 FGALLPCKECSGQLV
K321 GDVTAWTKCMVKTQN
K332 KTQNPSRKEWVTPKG
K338 RKEWVTPKGFREISY
S363 RIFPPESSAPAPLAL
- gap
- gap
S373 APLALPLSVTSAPTA
- gap
S376 ALPLSVTSAPTAVNS
S392 APADKPLSNMKILTL
K395 DKPLSNMKILTLGKL
K401 MKILTLGKLSQNKDE
K401 MKILTLGKLSQNKDE
K415 EAKAVIEKLGGKLTG
K426 KLTGSANKASLCIST
K434 ASLCISTKKEVEKMS
K435 SLCISTKKEVEKMSk
K442-sc KEVEKMSkkMEEVKA
K443-sc EVEKMSkkMEEVKAA
K448 SkkMEEVKAANVRVV
S466 FLQDVSASTkSLQEL
K468-ub QDVSASTkSLQELLS
K487 SSWGAEVKAEPGEVV
K487 SSWGAEVKAEPGEVV
K499 EVVAPKGKSAAPSKK
S500 VVAPKGKSAAPSKKS
K505 GKSAAPSKKSKGAVK
K508 AAPSKKSKGAVKEEG
K508 AAPSKKSKGAVKEEG
K518 VKEEGVNKSEkRMKL
K521-ac EGVNKSEkRMKLTLK
K524 NKSEkRMKLTLKGGA
K528 kRMKLTLKGGAAVDP
K548 HSAHVLEKGGKVFSA
K548 HSAHVLEKGGKVFSA
K551 HVLEKGGKVFSATLG
R564 LGLVDIVRGTNSYYK
K579 LQLLEDDKESRYWIF
K600 GTVIGSNKLEQMPSK
K600 GTVIGSNKLEQMPSK
S606 NKLEQMPSKEDAVEH
Y618 VEHFMKLYEEKTGNA
K621 FMKLYEEKTGNAWHS
K637 NFTKYPKKFYPLEID
T661 KLTVKPGTKSKLPKP
K662 LTVKPGTKSKLPKPV
K664 VKPGTKSKLPKPVQE
K667 GTKSKLPKPVQELVG
- gap
S681 GMIFDVESMKKALVE
K695 EYEIDLQKMPLGKLS
S733 ESQILDLSNRFYTLI
K748 PHDFGMKKPPLLNNA
Y775 LLDIEVAYSLLRGGS
S782 YSLLRGGSDDSSKDP
S785 LRGGSDDSSKDPIDV
S786 RGGSDDSSKDPIDVN
K787 GGSDDSSKDPIDVNY
K796 PIDVNYEKLKTDIKV
K798 DVNYEKLKTDIKVVD
S808 IKVVDRDSEEAEVIR
T824 YVKNTHATTHNAYDL
K838 LEVIDIFKIEREGES
R852 SQRYKPFRQLHNRRL
S874 TNFAGILSQGLRIAP
K940 KHASHISKLPKGKHS
K949 PKGKHSVKGLGKTTP
K1010 LKLKFNFKTSLW___
  human

 
S5-p ___MAESsDKLYRVE
S25-p RASCKKCsEsIPKDs
S27-p SCKKCsEsIPKDsLR
S32-p sEsIPKDsLRMAIMV
S41-p RMAIMVQsPMFDGKV
K87-ub DDQQKVKktAEAGGV
T88-p DQQKVKktAEAGGVT
K97-ac EAGGVTGkGQDGIGS
K97-ub EAGGVTGkGQDGIGS
K105-ac GQDGIGSkAEkTLGD
K108-ac GIGSkAEkTLGDFAA
K108-ub GIGSkAEkTLGDFAA
K119-ub DFAAEYAkSNRSTCK
K131-ac TCKGCMEkIEKGQVR
K148-ac KKMVDPEkPQLGMID
K165-ub YHPGCFVkNREELGF
S177-p LGFRPEYsAsQLKGF
S179-p FRPEYsAsQLKGFsL
S185-p AsQLKGFsLLAtEDK
T189-p KGFsLLAtEDKEALK
K203-sm KKQLPGVkSEGKRkG
K209-ub VkSEGKRkGDEVDGV
K221-ub DGVDEVAkKKsKKEK
S224-p DEVAkKKsKKEKDKD
K239-ub SKLEKALkAQNDLIW
K249-ac NDLIWNIkDELkkVC
K249-ub NDLIWNIkDELkkVC
K253-ac WNIkDELkkVCsTND
K254-ac NIkDELkkVCsTNDL
K254-ub NIkDELkkVCsTNDL
S257-p DELkkVCsTNDLkEL
K262-ub VCsTNDLkELLIFNk
K269-ub kELLIFNkQQVPsGE
S274-p FNkQQVPsGEsAILD
S277-p QQVPsGEsAILDRVA
E296 FGALLPCEECSGQLV
K320-ub GDVTAWTkCMVKTQT
K331-ub KTQTPNRkEWVTPkE
K337-ub RkEWVTPkEFREISY
S362-p RIFPPETsAsVAAtP
S364-p FPPETsAsVAAtPPP
T368-p TsAsVAAtPPPstAs
S372-p VAAtPPPstAsAPAA
T373-p AAtPPPstAsAPAAV
S375-p tPPPstAsAPAAVNS
S391-p ASADKPLsNMkILTL
K394-ub DKPLsNMkILTLGkL
K400-ac MkILTLGkLSRNKDE
K400-ub MkILTLGkLSRNKDE
K414-ub EVKAMIEkLGGKLTG
K425-ub KLTGTANkASLCIST
K433-ac ASLCISTkkEVEKMN
K434-ub SLCISTkkEVEKMNK
K441 kEVEKMNKKMEEVkE
K442 EVEKMNKKMEEVkEA
K447-ub NKKMEEVkEANIRVV
S465-p FLQDVSAsTKSLQEL
K467 QDVSAsTKSLQELFL
K486-ub SPWGAEVkAEPVEVV
K486-sm SPWGAEVkAEPVEVV
K498-ac EVVAPRGksGAALSk
S499-p VVAPRGksGAALSkK
K505-ac ksGAALSkKSkGQVK
K508-ac AALSkKSkGQVKEEG
K508-m1 AALSkKSkGQVKEEG
K518-ac VKEEGINkSEkRMkL
K521-ac EGINkSEkRMkLTLk
K524-ac NkSEkRMkLTLkGGA
K528-ub kRMkLTLkGGAAVDP
K548 HSAHVLEKGGkVFSA
K548-ub HSAHVLEkGGkVFSA
K551-ub HVLEkGGkVFSATLG
K564-ub LGLVDIVkGTNSYYK
K579-ub LQLLEDDkENRYWIF
K600-ac GTVIGSNkLEQMPsK
K600-ub GTVIGSNkLEQMPsK
S606-p NkLEQMPsKEDAIEH
Y618 IEHFMKLYEEkTGNA
K621-ac FMKLYEEkTGNAWHS
K637-ub NFTKYPKkFYPLEID
T661-p KLTVNPGtkSkLPkP
K662-ub LTVNPGtkSkLPkPV
K664-ub VNPGtkSkLPkPVQD
K667-ub GtkSkLPkPVQDLIk
K674-m2 kPVQDLIkMIFDVEs
S681-p kMIFDVEsMKKAMVE
K695-ub EYEIDLQkMPLGKLS
S733-p DSQILDLsNRFYTLI
K748-ub PHDFGMKkPPLLNNA
Y775-p LLDIEVAySLLRGGs
S782-p ySLLRGGsDDsskDP
S785-p LRGGsDDsskDPIDV
S786-p RGGsDDsskDPIDVN
K787-ub GGsDDsskDPIDVNY
K796-ub PIDVNYEkLkTDIKV
K798-ub DVNYEkLkTDIKVVD
S808-p IKVVDRDsEEAEIIR
T824-p YVKNTHAtTHNAYDL
K838-ub LEVIDIFkIEREGEC
K852-ub CQRYKPFkQLHNRRL
S874-p TNFAGILsQGLRIAP
K940-ub KHASHISkLPKGKHS
K949-ub PKGKHSVkGLGKTTP
K1010-ub LKLKFNFkTSLW___
  rat

 
T5 ___MAEATERLYRVE
S25 RASCKKCSESIPKDS
S27 SCKKCSESIPKDSLR
S32 SESIPKDSLRMAIMV
S41 RMAIMVQSPMFDGKV
K87 DDQQKVKKTAEAGGV
T88 DQQKVKKTAEAGGVA
K97 EAGGVAGKGQHGGGG
K97 EAGGVAGKGQHGGGG
K105 GQHGGGGKAEKTLGD
K108 GGGGKAEKTLGDFAA
K108 GGGGKAEKTLGDFAA
K119 DFAAEYAKSNRSTCK
K131 TCKGCMEKIEKGQMR
K148 KKMLDPEKPQLGMID
K165 YHPTCFVKNRDELGF
S177 LGFRPEYSASQLKGF
S179 FRPEYSASQLKGFSL
S185 ASQLKGFSLLSAEDK
A189 KGFSLLSAEDKEALK
K203 KKQLPAVKSEGKRKC
K209 VKSEGKRKCDEVDGI
K221 DGIDEVAKKKSKKGK
S224 DEVAKKKSKKGKDKE
K240 SKLEKALKAQNELVW
K250 NELVWNIKDELKKAC
K250 NELVWNIKDELKKAC
K254 WNIKDELKKACSTND
K255 NIKDELKKACSTNDL
K255 NIKDELKKACSTNDL
S258 DELKKACSTNDLKEL
K263 ACSTNDLKELLIFNQ
Q270 KELLIFNQQQVPSGE
S275 FNQQQVPSGESAILD
S278 QQVPSGESAILDRVA
K297 FGALLPCKECSGQLV
K321 GDVTAWTKCMVKTQN
K332 KTQNPSRKEWVTPKE
K338 RKEWVTPKEFREISY
S363 RLFPPESSAPAPPAP
- gap
- gap
S373 APPAPPVSITSAPTA
- gap
S376 APPVSITSAPTAVNS
S392 APADKPLSNMKILTL
K395 DKPLSNMKILTLGKL
K401 MKILTLGKLSQNKDE
K401 MKILTLGKLSQNKDE
K415 EAKAMIEKLGGKLTG
K426 KLTGSANKASLCIST
K434 ASLCISTKKEVEKMS
K435 SLCISTKKEVEKMSK
K442 KEVEKMSKKMEEVKA
K443 EVEKMSKKMEEVKAA
K448 SKKMEEVKAANVRVV
S466 FLQDVSASAKSLQEL
K468 QDVSASAKSLQELLS
K487 SSWGAEVKVEPGEVV
K487 SSWGAEVKVEPGEVV
K499 EVVVPKGKSAAPSKK
S500 VVVPKGKSAAPSKKS
K505 GKSAAPSKKSKGAVK
K508 AAPSKKSKGAVKEEG
K508 AAPSKKSKGAVKEEG
K518 VKEEGVNKSEKRMKL
K521 EGVNKSEKRMKLTLK
K524 NKSEKRMKLTLKGGA
K528 KRMKLTLKGGAAVDP
K548 HSAHVLEKGGKVFSA
K548 HSAHVLEKGGKVFSA
K551 HVLEKGGKVFSATLG
K564 LGLVDIVKGTNSYYK
K579 LQLLESDKESRYWIF
K600 GTVIGSNKLEQMPSK
K600 GTVIGSNKLEQMPSK
S606 NKLEQMPSKEDAVEH
Y618 VEHFMKLYEEKTGNA
K621 FMKLYEEKTGNAWHS
K637 NFTKYPKKFYPLEID
T661 KLAVKPGTKSKLPKP
K662 LAVKPGTKSKLPKPV
K664 VKPGTKSKLPKPVQE
K667 GTKSKLPKPVQELVG
- gap
S681 GMIFDVESMKKALVE
K695 EYEIDLQKMPLGKLS
S733 ESQILDLSNRFYTLI
K748 PHDFGMKKPPLLNNT
Y775 LLDIEVAYSLLRGGS
S782 YSLLRGGSDDSSKDP
S785 LRGGSDDSSKDPIDV
S786 RGGSDDSSKDPIDVN
K787 GGSDDSSKDPIDVNY
K796 PIDVNYEKLKTDIKV
K798 DVNYEKLKTDIKVVD
S808 IKVVDRDSEEAEVIR
T824 YVKNTHATTHNAYDL
K838 LEVIDIFKIEREGES
R852 SQRYKPFRQLHNRRL
S874 TNFAGILSQGLRIAP
K940 KHASHISKLPKGKHS
K949 PKGKHSVKGLGKTAP
K1010 LKLKFNFKTSLW___
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