Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Protein Page:
PARP1 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
PARP1 Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP- ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423. Interacts (when poly-ADP- ribosylated) with CHD1L. Interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression. Interacts with EEF1A1, RNF4 and TXK. Note: This description may include information from UniProtKB.
Protein type: Nuclear receptor co-regulator; EC 2.4.2.30; Nuclear envelope; DNA repair, damage; Transferase
Cellular Component: nucleoplasm; transcription factor complex; nucleolus; nuclear envelope; nucleus
Molecular Function: identical protein binding; protein binding; enzyme binding; DNA binding; zinc ion binding; protein N-terminus binding; transcription factor binding; NAD binding; NAD+ ADP-ribosyltransferase activity
Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; macrophage differentiation; transcription, DNA-dependent; DNA damage response, detection of DNA damage; negative regulation of transcription from RNA polymerase II promoter; DNA repair; protein autoprocessing; protein amino acid ADP-ribosylation; cellular response to insulin stimulus; base-excision repair; double-strand break repair; transforming growth factor beta receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter; regulation of growth rate; telomere maintenance
Reference #:  P09874 (UniProtKB)
Alt. Names/Synonyms: ADP-ribosyltransferase NAD(+); ADPRT; ADPRT 1; ADPRT1; NAD(+) ADP-ribosyltransferase 1; pADPRT-1; PARP; PARP-1; PARP1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly (ADP-ribose) polymerase); Poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; PPOL
Gene Symbols: PARP1
Molecular weight: 113,084 Da
Basal Isoelectric point: 8.99  Predict pI for various phosphorylation states
CST Pathways:  Death Receptor Signaling  |  NF-kB Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PARP1

Protein Structure Not Found.


STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho3D  |  Source  |  NURSA  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene


Sites Implicated In
enzymatic activity, induced: S372‑p, T373‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S5-p ___MAESsDKLYRVE
0 3 S41-p RMAIMVQsPMFDGKV
0 3 K97-a EAGGVTGkGQDGIGS
0 3 K105-a GQDGIGSkAEkTLGD
0 1 K108-u GIGSkAEkTLGDFAA
0 8 K119-u DFAAEYAkSNRSTCK
0 1 K131-a TCKGCMEkIEKGQVR
0 2 K165-u YHPGCFVkNREELGF
1 2 S177-p LGFRPEYsAsQLKGF
0 4 S179-p FRPEYsAsQLKGFsL
0 1 S185-p AsQLKGFsLLATEDK
1 1 K203-s KKQLPGVkSEGKRkG
0 1 K209-u VkSEGKRkGDEVDGV
0 1 K221-u DGVDEVAkKKSKKEK
0 1 K239-u SKLEKALkAQNDLIW
0 3 K249-a NDLIWNIkDELkKVC
0 7 K253-a WNIkDELkKVCSTND
0 3 K262-u VCSTNDLkELLIFNk
0 36 K269-u kELLIFNkQQVPsGE
0 2 S274-p FNkQQVPsGEsAILD
0 1 S277-p QQVPsGEsAILDRVA
0 1 K320-u GDVTAWTkCMVKTQT
0 1 K337-u RKEWVTPkEFREISY
0 1 S364-p FPPETSAsVAAtPPP
0 2 T368-p TSAsVAAtPPPstAS
2 2 S372-p VAAtPPPstASAPAA
2 1 T373-p AAtPPPstASAPAAV
0 1 K394-u DKPLSNMkILTLGkL
0 2 K400-a MkILTLGkLSRNKDE
0 6 K400-u MkILTLGkLSRNKDE
0 2 K414-u EVKAMIEkLGGKLTG
0 2 K425-u KLTGTANkASLCIST
0 3 K433-a ASLCISTkKEVEKMN
0 7 K447-u NKKMEEVkEANIRVV
0 3 K467 QDVSASTKSLQELFL
3 1 K486-s SPWGAEVkAEPVEVV
1 0 K498-a EVVAPRGkSGAALSk
1 0 K505-a kSGAALSkKSkGQVK
1 0 K508-a AALSkKSkGQVKEEG
1 6 K508-m1 AALSkKSkGQVKEEG
0 3 K518-a VKEEGINkSEkRMkL
1 26 K521-a EGINkSEkRMkLTLk
1 0 K524-a NkSEkRMkLTLkGGA
0 2 K528-u kRMkLTLkGGAAVDP
0 13 K548-u HSAHVLEkGGkVFSA
0 1 K551-u HVLEkGGkVFSATLG
0 5 K564-u LGLVDIVkGTNSYYK
0 2 K579-u LQLLEDDkENRYWIF
0 1 K600-a GTVIGSNkLEQMPsK
0 1 K600-u GTVIGSNkLEQMPsK
0 1 S606-p NkLEQMPsKEDAIEH
0 2 K621-a FMKLYEEkTGNAWHS
0 1 K637-u NFTKYPKkFYPLEID
0 1 K667-u GTKSKLPkPVQDLIK
0 5 K748-u PHDFGMKkPPLLNNA
0 1 Y775-p LLDIEVAySLLRGGs
1 14 S782-p ySLLRGGsDDsskDP
1 0 S785-p LRGGsDDsskDPIDV
1 1 S786-p RGGsDDsskDPIDVN
0 3 K787-u GGsDDsskDPIDVNY
0 5 K796-u PIDVNYEkLKTDIKV
0 3 K852-u CQRYKPFkQLHNRRL
0 6 K940-u KHASHISkLPKGKHS
0 7 K949-u PKGKHSVkGLGKTTP
0 2 K1010-u LKLKFNFkTSLW___
  mouse

► Hide Isoforms
 
S5 ___MAEASERLYRVQ
S41 RMAIMVQSPMFDGKV
K97 EAGGVAGKGQDGSGG
K105-a GQDGSGGkAEKTLGD
K108 GSGGkAEKTLGDFAA
K119 DFAAEYAKSNRSMCK
K131 MCKGCLEKIEKGQMR
K165 YHPTCFVKKRDELGF
S177 LGFRPEYSASQLKGF
S179 FRPEYSASQLKGFSL
S185 ASQLKGFSLLSAEDK
K203 KKQLPAIKNEGKRKG
K209 IKNEGKRKGDEVDGT
K221 DGTDEVAKKKSRKET
K239 SKLEKALKAQNELIW
K249 NELIWNIKDELKKAC
K253 WNIKDELKKACSTND
K262 ACSTNDLKELLIFNQ
Q269 KELLIFNQQQVPSGE
S274 FNQQQVPSGESAILD
S277 QQVPSGESAILDRVA
K320 GDVTAWTKCMVKTQN
K337 RKEWVTPKEFREISY
- gap
H368 SSAPITVHWPLSVTS
S372 ITVHWPLSVTSAPTA
- gap
K394 DKPLSNMKILTLGKL
K400 MKILTLGKLSQNKDE
K400 MKILTLGKLSQNKDE
K414 EAKAVIEKLGGKLTG
K425 KLTGSANKASLCISI
K433 ASLCISIKKEVEKMN
K447 NKKMEEVKEANIRVV
K467 QDVSASTKSLQDLLS
K486 SPWGAEVKAEPGEVV
K498 EVVAPRGKSAAPSKK
K504 GKSAAPSKKSKGCFK
K507 AAPSKKSKGCFKEEG
K507 AAPSKKSKGCFKEEG
K517 FKEEGVNKSEKRMKL
K520 EGVNKSEKRMKLTLK
K523 NKSEKRMKLTLKGGA
K527 KRMKLTLKGGAAVDP
K547-u HSAHVLEkGGKVFSA
K550 HVLEkGGKVFSATLG
K563-u LGLVDIVkGTNSYYK
K578-u LQLLEDDkESRYWIF
K599 GTVIGSNKLEQMPSK
K599 GTVIGSNKLEQMPSK
S605 NKLEQMPSKEEAVEQ
K620 FMKLYEEKTGNAWHS
K636 NFTKYPKKFYPLEID
K666 GTKSKLPKPVQELVG
K747 PHDFGMKKPPLLNNA
Y774 LLDIEVAYSLLRGGs
S781-p YSLLRGGsDDSSKDP
S784 LRGGsDDSSKDPIDV
S785 RGGsDDSSKDPIDVN
K786 GGsDDSSKDPIDVNY
K795 PIDVNYEKLKTDIKV
R851 SQRYKPFRQLHNRRL
K939 KHASHISKLPKGKHS
K948 PKGKHSVKGLGKTTP
K1009 LKLKFNFKTSLW___
  PARP1 iso3  
S5 ___MAEASERLYRVE
S41 RMTIMVQSPMFDGKV
K97 EAGGVAGKGQDGSGG
K105 GQDGSGGKAEKTLGD
K108 GSGGKAEKTLGDFLA
K119-u DFLAEYAkSNRSMCK
K131 MCKGCLEKIEKGQMR
K165 YHPTCFVKKRDELGF
S177 LGFRPEYSASQLKGF
S179 FRPEYSASQLKGFSL
S185 ASQLKGFSLLSAEDK
K203 KKQLPAIKNEGKRKG
K209 IKNEGKRKGDEVDGT
K221 DGTDEVAKKKSKKGK
K240 SKLEKALKAQNELIW
K250 NELIWNIKDELKKAC
K254 WNIKDELKKACSTND
K263 ACSTNDLKELLIFNQ
Q270 KELLIFNQQQVPSGE
S275 FNQQQVPSGESAILD
S278 QQVPSGESAILDRVA
K321 GDVTAWTKCMVKTQN
K338 RKEWVTPKGFREISY
- gap
- gap
S373 APLALPLSVTSAPTA
- gap
K395 DKPLSNMKILTLGKL
K401 MKILTLGKLSQNKDE
K401 MKILTLGKLSQNKDE
K415 EAKAVIEKLGGKLTG
K426 KLTGSANKASLCIST
K434 ASLCISTKKEVEKMS
K448 SKKMEEVKAANVRVV
K468-u QDVSASTkSLQELLS
K487 SSWGAEVKAEPGEVV
K499 EVVAPKGKSAAPSKK
K505 GKSAAPSKKSKGAVK
K508 AAPSKKSKGAVKEEG
K508 AAPSKKSKGAVKEEG
K518 VKEEGVNKSEkRMKL
K521-a EGVNKSEkRMKLTLK
K524 NKSEkRMKLTLKGGA
K528 kRMKLTLKGGAAVDP
K548 HSAHVLEKGGKVFSA
K551 HVLEKGGKVFSATLG
R564 LGLVDIVRGTNSYYK
K579 LQLLEDDKESRYWIF
K600 GTVIGSNKLEQMPSK
K600 GTVIGSNKLEQMPSK
S606 NKLEQMPSKEDAVEH
K621 FMKLYEEKTGNAWHS
K637 NFTKYPKKFYPLEID
K667 GTKSKLPKPVQELVG
K748 PHDFGMKKPPLLNNA
Y775 LLDIEVAYSLLRGGS
S782 YSLLRGGSDDSSKDP
S785 LRGGSDDSSKDPIDV
S786 RGGSDDSSKDPIDVN
K787 GGSDDSSKDPIDVNY
K796 PIDVNYEKLKTDIKV
R852 SQRYKPFRQLHNRRL
K940 KHASHISKLPKGKHS
K949 PKGKHSVKGLGKTTP
K1010 LKLKFNFKTSLW___
  rat

 
T5 ___MAEATERLYRVE
S41 RMAIMVQSPMFDGKV
K97 EAGGVAGKGQHGGGG
K105 GQHGGGGKAEKTLGD
K108 GGGGKAEKTLGDFAA
K119 DFAAEYAKSNRSTCK
K131 TCKGCMEKIEKGQMR
K165 YHPTCFVKNRDELGF
S177 LGFRPEYSASQLKGF
S179 FRPEYSASQLKGFSL
S185 ASQLKGFSLLSAEDK
K203 KKQLPAVKSEGKRKC
K209 VKSEGKRKCDEVDGI
K221 DGIDEVAKKKSKKGK
K240 SKLEKALKAQNELVW
K250 NELVWNIKDELKKAC
K254 WNIKDELKKACSTND
K263 ACSTNDLKELLIFNQ
Q270 KELLIFNQQQVPSGE
S275 FNQQQVPSGESAILD
S278 QQVPSGESAILDRVA
K321 GDVTAWTKCMVKTQN
K338 RKEWVTPKEFREISY
- gap
- gap
S373 APPAPPVSITSAPTA
- gap
K395 DKPLSNMKILTLGKL
K401 MKILTLGKLSQNKDE
K401 MKILTLGKLSQNKDE
K415 EAKAMIEKLGGKLTG
K426 KLTGSANKASLCIST
K434 ASLCISTKKEVEKMS
K448 SKKMEEVKAANVRVV
K468 QDVSASAKSLQELLS
K487 SSWGAEVKVEPGEVV
K499 EVVVPKGKSAAPSKK
K505 GKSAAPSKKSKGAVK
K508 AAPSKKSKGAVKEEG
K508 AAPSKKSKGAVKEEG
K518 VKEEGVNKSEKRMKL
K521 EGVNKSEKRMKLTLK
K524 NKSEKRMKLTLKGGA
K528 KRMKLTLKGGAAVDP
K548 HSAHVLEKGGKVFSA
K551 HVLEKGGKVFSATLG
K564 LGLVDIVKGTNSYYK
K579 LQLLESDKESRYWIF
K600 GTVIGSNKLEQMPSK
K600 GTVIGSNKLEQMPSK
S606 NKLEQMPSKEDAVEH
K621 FMKLYEEKTGNAWHS
K637 NFTKYPKKFYPLEID
K667 GTKSKLPKPVQELVG
K748 PHDFGMKKPPLLNNT
Y775 LLDIEVAYSLLRGGS
S782 YSLLRGGSDDSSKDP
S785 LRGGSDDSSKDPIDV
S786 RGGSDDSSKDPIDVN
K787 GGSDDSSKDPIDVNY
K796 PIDVNYEKLKTDIKV
R852 SQRYKPFRQLHNRRL
K940 KHASHISKLPKGKHS
K949 PKGKHSVKGLGKTAP
K1010 LKLKFNFKTSLW___
Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.