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Protein Page:
PARP1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PARP1 Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP- ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423. Interacts (when poly-ADP- ribosylated) with CHD1L. Interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression. Interacts with EEF1A1, RNF4 and TXK. Note: This description may include information from UniProtKB.
Protein type: Nucleolus; Transferase; DNA repair, damage; EC 2.4.2.30; Nuclear envelope; Nuclear receptor co-regulator
Cellular Component: nucleoplasm; transcription factor complex; membrane; nucleolus; nuclear envelope; nucleus
Molecular Function: identical protein binding; protein binding; enzyme binding; DNA binding; zinc ion binding; protein N-terminus binding; NAD binding; transcription factor binding; NAD+ ADP-ribosyltransferase activity
Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; macrophage differentiation; transcription, DNA-dependent; DNA damage response, detection of DNA damage; negative regulation of transcription from RNA polymerase II promoter; DNA repair; protein autoprocessing; protein amino acid ADP-ribosylation; cellular response to insulin stimulus; base-excision repair; double-strand break repair; transforming growth factor beta receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter; regulation of growth rate; telomere maintenance
Reference #:  P09874 (UniProtKB)
Alt. Names/Synonyms: ADP-ribosyltransferase NAD(+); ADPRT; ADPRT 1; ADPRT1; NAD(+) ADP-ribosyltransferase 1; pADPRT-1; PARP; PARP-1; PARP1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly (ADP-ribose) polymerase); Poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; PPOL
Gene Symbols: PARP1
Molecular weight: 113,084 Da
Basal Isoelectric point: 8.99  Predict pI for various phosphorylation states
CST Pathways:  Death Receptor Signaling  |  NF-kB Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PARP1

Protein Structure Not Found.


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Sites Implicated In
enzymatic activity, induced: S372‑p, T373‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S5-p ___MAESsDKLYRVE
0 1 S25-p RASCKKCsEsIPKDs
0 1 S27-p SCKKCsEsIPKDsLR
0 1 S32-p sEsIPKDsLRMAIMV
0 4 S41-p RMAIMVQsPMFDGKV
0 1 K87-ub DDQQKVKktAEAGGV
0 2 T88-p DQQKVKktAEAGGVT
0 3 K97-ac EAGGVTGkGQDGIGS
0 1 K97-ub EAGGVTGkGQDGIGS
0 3 K105-ac GQDGIGSkAEkTLGD
0 1 K108-ac GIGSkAEkTLGDFAA
0 1 K108-ub GIGSkAEkTLGDFAA
0 8 K119-ub DFAAEYAkSNRSTCK
0 1 K131-ac TCKGCMEkIEKGQVR
0 1 K148-ac KKMVDPEkPQLGMID
0 2 K165-ub YHPGCFVkNREELGF
1 2 S177-p LGFRPEYsAsQLKGF
0 4 S179-p FRPEYsAsQLKGFsL
0 2 S185-p AsQLKGFsLLAtEDK
0 1 T189-p KGFsLLAtEDKEALK
1 1 K203-sm KKQLPGVkSEGKRkG
0 1 K209-ub VkSEGKRkGDEVDGV
0 1 K221-ub DGVDEVAkKKsKKEK
0 1 S224-p DEVAkKKsKKEKDKD
0 1 K239-ub SKLEKALkAQNDLIW
0 3 K249-ac NDLIWNIkDELkkVC
0 1 K249-ub NDLIWNIkDELkkVC
0 7 K253-ac WNIkDELkkVCsTND
0 1 K254-ac NIkDELkkVCsTNDL
0 1 K254-ub NIkDELkkVCsTNDL
0 1 S257-p DELkkVCsTNDLkEL
0 3 K262-ub VCsTNDLkELLIFNk
0 36 K269-ub kELLIFNkQQVPsGE
0 3 S274-p FNkQQVPsGEsAILD
0 1 S277-p QQVPsGEsAILDRVA
0 1 E296 FGALLPCEECSGQLV
0 1 K320-ub GDVTAWTkCMVKTQT
0 1 K331-ub KTQTPNRkEWVTPkE
0 1 K337-ub RkEWVTPkEFREISY
0 1 S362-p RIFPPETsAsVAAtP
0 2 S364-p FPPETsAsVAAtPPP
0 4 T368-p TsAsVAAtPPPstAs
2 2 S372-p VAAtPPPstAsAPAA
2 1 T373-p AAtPPPstAsAPAAV
0 1 S375-p tPPPstAsAPAAVNS
0 1 S391-p ASADKPLsNMkILTL
0 1 K394-ub DKPLsNMkILTLGkL
0 2 K400-ac MkILTLGkLSRNKDE
0 6 K400-ub MkILTLGkLSRNKDE
0 2 K414-ub EVKAMIEkLGGKLTG
0 2 K425-ub KLTGTANkASLCIST
0 3 K433-ac ASLCISTkkEVEKMN
0 1 K434-ub SLCISTkkEVEKMNK
0 1 K441 kEVEKMNKKMEEVkE
0 1 K442 EVEKMNKKMEEVkEA
0 7 K447-ub NKKMEEVkEANIRVV
1 0 S465-p FLQDVSAsTKSLQEL
0 3 K467 QDVSAsTKSLQELFL
0 1 K486-ub SPWGAEVkAEPVEVV
3 1 K486-sm SPWGAEVkAEPVEVV
1 0 K498-ac EVVAPRGksGAALSk
0 1 S499-p VVAPRGksGAALSkK
1 0 K505-ac ksGAALSkKSkGQVK
1 0 K508-ac AALSkKSkGQVKEEG
1 6 K508-m1 AALSkKSkGQVKEEG
0 3 K518-ac VKEEGINkSEkRMkL
1 26 K521-ac EGINkSEkRMkLTLk
1 0 K524-ac NkSEkRMkLTLkGGA
0 2 K528-ub kRMkLTLkGGAAVDP
0 1 K548 HSAHVLEKGGkVFSA
0 13 K548-ub HSAHVLEkGGkVFSA
0 1 K551-ub HVLEkGGkVFSATLG
0 5 K564-ub LGLVDIVkGTNSYYK
0 2 K579-ub LQLLEDDkENRYWIF
0 1 K600-ac GTVIGSNkLEQMPsK
0 1 K600-ub GTVIGSNkLEQMPsK
0 1 S606-p NkLEQMPsKEDAIEH
0 1 Y618 IEHFMKLYEEkTGNA
0 3 K621-ac FMKLYEEkTGNAWHS
0 1 K637-ub NFTKYPKkFYPLEID
0 1 T661-p KLTVNPGtkSkLPkP
0 1 K662-ub LTVNPGtkSkLPkPV
0 1 K664-ub VNPGtkSkLPkPVQD
0 1 K667-ub GtkSkLPkPVQDLIk
0 1 K674-m2 kPVQDLIkMIFDVEs
0 1 S681-p kMIFDVEsMKKAMVE
0 1 K695-ub EYEIDLQkMPLGKLS
0 1 S733-p DSQILDLsNRFYTLI
0 5 K748-ub PHDFGMKkPPLLNNA
0 1 Y775-p LLDIEVAySLLRGGs
2 17 S782-p ySLLRGGsDDsskDP
2 0 S785-p LRGGsDDsskDPIDV
1 1 S786-p RGGsDDsskDPIDVN
0 3 K787-ub GGsDDsskDPIDVNY
0 5 K796-ub PIDVNYEkLkTDIKV
0 1 K798-ub DVNYEkLkTDIKVVD
0 1 S808-p IKVVDRDsEEAEIIR
0 1 T824-p YVKNTHAtTHNAYDL
0 1 K838-ub LEVIDIFkIEREGEC
0 3 K852-ub CQRYKPFkQLHNRRL
0 1 S874-p TNFAGILsQGLRIAP
0 6 K940-ub KHASHISkLPKGKHS
0 7 K949-ub PKGKHSVkGLGKTTP
0 2 K1010-ub LKLKFNFkTSLW___
  mouse

► Hide Isoforms
 
S5 ___MAEASERLYRVQ
S25 RASCKKCSESIPKDS
S27 SCKKCSESIPKDSLR
S32 SESIPKDSLRMAIMV
S41 RMAIMVQSPMFDGKV
K87 DDQQKVKKtAEAGGV
T88-p DQQKVKKtAEAGGVA
K97 EAGGVAGKGQDGSGG
K97 EAGGVAGKGQDGSGG
K105-ac GQDGSGGkAEKTLGD
K108 GSGGkAEKTLGDFAA
K108 GSGGkAEKTLGDFAA
K119 DFAAEYAKSNRSMCK
K131 MCKGCLEKIEKGQMR
K148 KKMVDPEKPQLGMID
K165 YHPTCFVKKRDELGF
S177 LGFRPEYSASQLKGF
S179 FRPEYSASQLKGFSL
S185 ASQLKGFSLLSAEDK
A189 KGFSLLSAEDKEALK
K203 KKQLPAIKNEGKRKG
K209 IKNEGKRKGDEVDGT
K221 DGTDEVAKKKSRKET
S224 DEVAKKKSRKETDKY
K239 SKLEKALKAQNELIW
K249 NELIWNIKDELKKAC
K249 NELIWNIKDELKKAC
K253 WNIKDELKKACSTND
K254 NIKDELKKACSTNDL
K254 NIKDELKKACSTNDL
S257 DELKKACSTNDLKEL
K262 ACSTNDLKELLIFNQ
Q269 KELLIFNQQQVPSGE
S274 FNQQQVPSGESAILD
S277 QQVPSGESAILDRVA
K296-ub FGALLPCkECSGQLV
K320 GDVTAWTKCMVKTQN
K331 KTQNPSRKEWVTPKE
K337 RKEWVTPKEFREISY
S362 RIFPPESSAPITVHW
- gap
H368 SSAPITVHWPLSVTS
S372 ITVHWPLSVTSAPTA
- gap
S375 HWPLSVTSAPTAVNS
S391 APADKPLSNMKILTL
K394 DKPLSNMKILTLGKL
K400 MKILTLGKLSQNKDE
K400 MKILTLGKLSQNKDE
K414 EAKAVIEKLGGKLTG
K425 KLTGSANKASLCISI
K433 ASLCISIKKEVEKMN
K434 SLCISIKKEVEKMNK
K441 KEVEKMNKKMEEVKE
K442 EVEKMNKKMEEVKEA
K447 NKKMEEVKEANIRVV
S465 FLQDVSASTKSLQDL
K467 QDVSASTKSLQDLLS
K486 SPWGAEVKAEPGEVV
K486 SPWGAEVKAEPGEVV
K498 EVVAPRGKSAAPSKK
S499 VVAPRGKSAAPSKKS
K504 GKSAAPSKKSKGCFK
K507 AAPSKKSKGCFKEEG
K507 AAPSKKSKGCFKEEG
K517 FKEEGVNKSEKRMKL
K520 EGVNKSEKRMKLTLK
K523 NKSEKRMKLTLKGGA
K527 KRMKLTLKGGAAVDP
K547-ac HSAHVLEkGGKVFSA
K547-ub HSAHVLEkGGKVFSA
K550 HVLEkGGKVFSATLG
K563-ub LGLVDIVkGTNSYYK
K578-ub LQLLEDDkESRYWIF
K599 GTVIGSNKLEQMPSK
K599 GTVIGSNKLEQMPSK
S605 NKLEQMPSKEEAVEQ
Y617-p VEQFMKLyEEKTGNA
K620 FMKLyEEKTGNAWHS
K636 NFTKYPKKFYPLEID
T660 KLTVKPGTKSKLPKP
K661 LTVKPGTKSKLPKPV
K663 VKPGTKSKLPKPVQE
K666 GTKSKLPKPVQELVG
- gap
S680 GMIFDVDSMKKALVE
K694 EYEIDLQKMPLGKLS
S732 ESQILDLSNRFYTLI
K747 PHDFGMKKPPLLNNA
Y774 LLDIEVAYSLLRGGs
S781-p YSLLRGGsDDSSKDP
S784 LRGGsDDSSKDPIDV
S785 RGGsDDSSKDPIDVN
K786 GGsDDSSKDPIDVNY
K795 PIDVNYEKLKTDIKV
K797 DVNYEKLKTDIKVVD
S807 IKVVDRDSEEAEVIR
T823 YVKNTHATTHNAYDL
K837 LEVIDIFKIEREGES
R851 SQRYKPFRQLHNRRL
S873 TNFAGILSQGLRIAP
K939 KHASHISKLPKGKHS
K948 PKGKHSVKGLGKTTP
K1009 LKLKFNFKTSLW___
  PARP1 iso3  
S5 ___MAEASERLYRVE
S25 RASCKKCSESIPKDS
S27 SCKKCSESIPKDSLR
S32 SESIPKDSLRMTIMV
S41 RMTIMVQSPMFDGKV
K87 DDQQKVKKTAEAGGV
T88 DQQKVKKTAEAGGVA
K97 EAGGVAGKGQDGSGG
K97 EAGGVAGKGQDGSGG
K105 GQDGSGGKAEKTLGD
K108 GSGGKAEKTLGDFLA
K108 GSGGKAEKTLGDFLA
K119-ub DFLAEYAkSNRSMCK
K131 MCKGCLEKIEKGQMR
K148 KKMVDPEKPQLGMID
K165 YHPTCFVKKRDELGF
S177 LGFRPEYSASQLKGF
S179 FRPEYSASQLKGFSL
S185 ASQLKGFSLLSAEDK
A189 KGFSLLSAEDKEALK
K203 KKQLPAIKNEGKRKG
K209 IKNEGKRKGDEVDGT
K221 DGTDEVAKKKSKKGK
S224 DEVAKKKSKKGKDKD
K240 SKLEKALKAQNELIW
K250 NELIWNIKDELKKAC
K250 NELIWNIKDELKKAC
K254 WNIKDELKKACSTND
K255 NIKDELKKACSTNDL
K255 NIKDELKKACSTNDL
S258 DELKKACSTNDLKEL
K263 ACSTNDLKELLIFNQ
Q270 KELLIFNQQQVPSGE
S275 FNQQQVPSGESAILD
S278 QQVPSGESAILDRVA
K297 FGALLPCKECSGQLV
K321 GDVTAWTKCMVKTQN
K332 KTQNPSRKEWVTPKG
K338 RKEWVTPKGFREISY
S363 RIFPPESSAPAPLAL
- gap
- gap
S373 APLALPLSVTSAPTA
- gap
S376 ALPLSVTSAPTAVNS
S392 APADKPLSNMKILTL
K395 DKPLSNMKILTLGKL
K401 MKILTLGKLSQNKDE
K401 MKILTLGKLSQNKDE
K415 EAKAVIEKLGGKLTG
K426 KLTGSANKASLCIST
K434 ASLCISTKKEVEKMS
K435 SLCISTKKEVEKMSk
K442-sc KEVEKMSkkMEEVKA
K443-sc EVEKMSkkMEEVKAA
K448 SkkMEEVKAANVRVV
S466 FLQDVSASTkSLQEL
K468-ub QDVSASTkSLQELLS
K487 SSWGAEVKAEPGEVV
K487 SSWGAEVKAEPGEVV
K499 EVVAPKGKSAAPSKK
S500 VVAPKGKSAAPSKKS
K505 GKSAAPSKKSKGAVK
K508 AAPSKKSKGAVKEEG
K508 AAPSKKSKGAVKEEG
K518 VKEEGVNKSEkRMKL
K521-ac EGVNKSEkRMKLTLK
K524 NKSEkRMKLTLKGGA
K528 kRMKLTLKGGAAVDP
K548 HSAHVLEKGGKVFSA
K548 HSAHVLEKGGKVFSA
K551 HVLEKGGKVFSATLG
R564 LGLVDIVRGTNSYYK
K579 LQLLEDDKESRYWIF
K600 GTVIGSNKLEQMPSK
K600 GTVIGSNKLEQMPSK
S606 NKLEQMPSKEDAVEH
Y618 VEHFMKLYEEKTGNA
K621 FMKLYEEKTGNAWHS
K637 NFTKYPKKFYPLEID
T661 KLTVKPGTKSKLPKP
K662 LTVKPGTKSKLPKPV
K664 VKPGTKSKLPKPVQE
K667 GTKSKLPKPVQELVG
- gap
S681 GMIFDVESMKKALVE
K695 EYEIDLQKMPLGKLS
S733 ESQILDLSNRFYTLI
K748 PHDFGMKKPPLLNNA
Y775 LLDIEVAYSLLRGGS
S782 YSLLRGGSDDSSKDP
S785 LRGGSDDSSKDPIDV
S786 RGGSDDSSKDPIDVN
K787 GGSDDSSKDPIDVNY
K796 PIDVNYEKLKTDIKV
K798 DVNYEKLKTDIKVVD
S808 IKVVDRDSEEAEVIR
T824 YVKNTHATTHNAYDL
K838 LEVIDIFKIEREGES
R852 SQRYKPFRQLHNRRL
S874 TNFAGILSQGLRIAP
K940 KHASHISKLPKGKHS
K949 PKGKHSVKGLGKTTP
K1010 LKLKFNFKTSLW___
  rat

 
T5 ___MAEATERLYRVE
S25 RASCKKCSESIPKDS
S27 SCKKCSESIPKDSLR
S32 SESIPKDSLRMAIMV
S41 RMAIMVQSPMFDGKV
K87 DDQQKVKKTAEAGGV
T88 DQQKVKKTAEAGGVA
K97 EAGGVAGKGQHGGGG
K97 EAGGVAGKGQHGGGG
K105 GQHGGGGKAEKTLGD
K108 GGGGKAEKTLGDFAA
K108 GGGGKAEKTLGDFAA
K119 DFAAEYAKSNRSTCK
K131 TCKGCMEKIEKGQMR
K148 KKMLDPEKPQLGMID
K165 YHPTCFVKNRDELGF
S177 LGFRPEYSASQLKGF
S179 FRPEYSASQLKGFSL
S185 ASQLKGFSLLSAEDK
A189 KGFSLLSAEDKEALK
K203 KKQLPAVKSEGKRKC
K209 VKSEGKRKCDEVDGI
K221 DGIDEVAKKKSKKGK
S224 DEVAKKKSKKGKDKE
K240 SKLEKALKAQNELVW
K250 NELVWNIKDELKKAC
K250 NELVWNIKDELKKAC
K254 WNIKDELKKACSTND
K255 NIKDELKKACSTNDL
K255 NIKDELKKACSTNDL
S258 DELKKACSTNDLKEL
K263 ACSTNDLKELLIFNQ
Q270 KELLIFNQQQVPSGE
S275 FNQQQVPSGESAILD
S278 QQVPSGESAILDRVA
K297 FGALLPCKECSGQLV
K321 GDVTAWTKCMVKTQN
K332 KTQNPSRKEWVTPKE
K338 RKEWVTPKEFREISY
S363 RLFPPESSAPAPPAP
- gap
- gap
S373 APPAPPVSITSAPTA
- gap
S376 APPVSITSAPTAVNS
S392 APADKPLSNMKILTL
K395 DKPLSNMKILTLGKL
K401 MKILTLGKLSQNKDE
K401 MKILTLGKLSQNKDE
K415 EAKAMIEKLGGKLTG
K426 KLTGSANKASLCIST
K434 ASLCISTKKEVEKMS
K435 SLCISTKKEVEKMSK
K442 KEVEKMSKKMEEVKA
K443 EVEKMSKKMEEVKAA
K448 SKKMEEVKAANVRVV
S466 FLQDVSASAKSLQEL
K468 QDVSASAKSLQELLS
K487 SSWGAEVKVEPGEVV
K487 SSWGAEVKVEPGEVV
K499 EVVVPKGKSAAPSKK
S500 VVVPKGKSAAPSKKS
K505 GKSAAPSKKSKGAVK
K508 AAPSKKSKGAVKEEG
K508 AAPSKKSKGAVKEEG
K518 VKEEGVNKSEKRMKL
K521 EGVNKSEKRMKLTLK
K524 NKSEKRMKLTLKGGA
K528 KRMKLTLKGGAAVDP
K548 HSAHVLEKGGKVFSA
K548 HSAHVLEKGGKVFSA
K551 HVLEKGGKVFSATLG
K564 LGLVDIVKGTNSYYK
K579 LQLLESDKESRYWIF
K600 GTVIGSNKLEQMPSK
K600 GTVIGSNKLEQMPSK
S606 NKLEQMPSKEDAVEH
Y618 VEHFMKLYEEKTGNA
K621 FMKLYEEKTGNAWHS
K637 NFTKYPKKFYPLEID
T661 KLAVKPGTKSKLPKP
K662 LAVKPGTKSKLPKPV
K664 VKPGTKSKLPKPVQE
K667 GTKSKLPKPVQELVG
- gap
S681 GMIFDVESMKKALVE
K695 EYEIDLQKMPLGKLS
S733 ESQILDLSNRFYTLI
K748 PHDFGMKKPPLLNNT
Y775 LLDIEVAYSLLRGGS
S782 YSLLRGGSDDSSKDP
S785 LRGGSDDSSKDPIDV
S786 RGGSDDSSKDPIDVN
K787 GGSDDSSKDPIDVNY
K796 PIDVNYEKLKTDIKV
K798 DVNYEKLKTDIKVVD
S808 IKVVDRDSEEAEVIR
T824 YVKNTHATTHNAYDL
K838 LEVIDIFKIEREGES
R852 SQRYKPFRQLHNRRL
S874 TNFAGILSQGLRIAP
K940 KHASHISKLPKGKHS
K949 PKGKHSVKGLGKTAP
K1010 LKLKFNFKTSLW___
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