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PARP1 (human)

Overview PARP1 Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP- ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423. Interacts (when poly-ADP- ribosylated) with CHD1L. Interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression. Interacts with EEF1A1, RNF4 and TXK. Note: This description may include information from UniProtKB. Protein type: EC 2.4.2.30; Transferase; DNA repair; Nuclear receptor co-regulator; Nuclear envelope Cellular Component: nucleoplasm; transcription factor complex; nucleolus; nuclear envelope; nucleus Molecular Function: identical protein binding; protein binding; DNA binding; zinc ion binding; protein N-terminus binding; transcription factor binding; NAD binding; NAD+ ADP-ribosyltransferase activity Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; DNA damage response, detection of DNA damage; negative regulation of transcription from RNA polymerase II promoter; DNA repair; protein autoprocessing; protein amino acid ADP-ribosylation; cellular response to insulin stimulus; base-excision repair; transforming growth factor beta receptor signaling pathway; gene expression; regulation of growth rate; telomere maintenance Reference #:  P09874 (UniProtKB) Alt. Names/Synonyms: ADP-ribosyltransferase (NAD+; ADP-ribosyltransferase NAD(+); ADPRT; ADPRT 1; ADPRT1; NAD(+) ADP-ribosyltransferase 1; pADPRT-1; PARP; PARP-1; PARP1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly (ADP-ribose) polymerase); Poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; PPOL Gene Symbols: PARP1 Molecular weight: 113,084 Da Basal Isoelectric point: 8.99  Predict pI for various phosphorylation states CST Pathways:  Death Receptor Signaling  |  NF-kB Signaling Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below PARP1 1UK0 - A/B=662-1011 (human) 1UK1 - A/B=662-1011 (human) 1WOK - A/B/C/D=662-1011 (human) 2COK - A=385-486 (human) 2CR9 - A=518-643 (human) 2CS2 - A=103-224 (human) 2DMJ - A=1-93 (human) 2JVN - A=233-358 (human) 2L30 - A=1-108 (human) 2L31 - A=103-214 (human) 2RCW - A=662-1011 (human) 2RD6 - A=662-1011 (human) 2RIQ - A=216-366 (human) 3GJW - A=662-1011 (human) 3GN7 - A=662-1011 (human) 3L3L - A=662-1011 (human) 3L3M - A=662-1011 (human) 3OD8 - F/A/E/B/H/C/D/G=2-96 (human) 3ODA - F/A/E/B/H/C/D/G=2-96 (human) 3ODC - A/B=105-206 (human) 3ODE - A/B=105-206 (human) 4AV1 - A/D/C/B=5-202 (human) 4DQY - A/D=1-96 E/B=216-366 F/C=518-1014 (human) 4HHY - A/D/C/B=660-1011 (human) 4HHZ - A/D/C/B=660-1011 (human) 2LE0 - A=389-487 (rat)

STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB 1UK0 1UK1 1WOK 2COK 2CR9 2CS2 2DMJ 2JVN 2L30 2L31 2RCW 2RD6 2RIQ 3GJW 3GN7 3L3L 3L3M 3OD8 3ODA 3ODC 3ODE 4AV1 4DQY 4HHY 4HHZ  |  ENZYME  |  Phospho3D  |  DISEASE  |  Source  |  NURSA  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene

	Sites Implicated In
enzymatic activity, induced: S372‑p, T373‑p

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
0	1		S5-p	___MAESsDKLYRVE
0	1		S25-p	RASCKKCsEsIPKDs
0	1		S27-p	SCKKCsEsIPKDsLR
0	1		S32-p	sEsIPKDsLRMAIMV
0	2		S41-p	RMAIMVQsPMFDGKV
0	1		K87-u	DDQQKVKkTAEAGGV
0	1		T88	DQQKVKkTAEAGGVT
0	3		K97-a	EAGGVTGkGQDGIGS
0	1		K97-u	EAGGVTGkGQDGIGS
0	3		K105-a	GQDGIGSkAEkTLGD
0	1		K108-u	GIGSkAEkTLGDFAA
0	8		K119-u	DFAAEYAkSNRSTCK
0	1		K131-a	TCKGCMEkIEKGQVR
0	2		K165-u	YHPGCFVkNREELGF
1	1		S177-p	LGFRPEYsAsQLKGF
0	3		S179-p	FRPEYsAsQLKGFSL
1	0		K203-s	KKQLPGVkSEGKRkG
0	1		K209-u	VkSEGKRkGDEVDGV
0	1		K221-u	DGVDEVAkKKsKKEK
0	1		S224-p	DEVAkKKsKKEKDKD
0	1		K239-u	SKLEKALkAQNDLIW
0	3		K249-a	NDLIWNIkDELkkVC
0	1		K249-u	NDLIWNIkDELkkVC
0	7		K253-a	WNIkDELkkVCSTND
0	1		K254-a	NIkDELkkVCSTNDL
0	1		K254-u	NIkDELkkVCSTNDL
0	3		K262-u	VCSTNDLkELLIFNk
0	36		K269-u	kELLIFNkQQVPsGE
0	1		S274-p	FNkQQVPsGESAILD
0	1		E296	FGALLPCEECSGQLV
0	1		K320-u	GDVTAWTkCMVKTQT
0	1		K331-u	KTQTPNRkEWVTPkE
0	1		K337-u	RkEWVTPkEFREISY
0	2		T368-p	TSASVAAtPPPstAs
1	2		S372-p	VAAtPPPstAsAPAA
1	1		T373-p	AAtPPPstAsAPAAV
0	1		S375-p	tPPPstAsAPAAVNS
0	1		K394-u	DKPLSNMkILTLGkL
0	2		K400-a	MkILTLGkLSRNKDE
0	6		K400-u	MkILTLGkLSRNKDE
0	2		K414-u	EVKAMIEkLGGKLTG
0	2		K425-u	KLTGTANkASLCIST
0	3		K433-a	ASLCISTkkEVEKMN
0	1		K434-u	SLCISTkkEVEKMNK
0	7		K447-u	NKKMEEVkEANIRVV
0	3		K467	QDVSASTKSLQELFL
0	1		K486-u	SPWGAEVkAEPVEVV
3	1		K486-s	SPWGAEVkAEPVEVV
1	0		K498-a	EVVAPRGksGAALSk
0	1		S499-p	VVAPRGksGAALSkK
1	0		K505-a	ksGAALSkKSkGQVK
1	0		K508-a	AALSkKSkGQVKEEG
0	6		K508-m1	AALSkKSkGQVKEEG
0	3		K518-a	VKEEGINkSEkRMkL
1	26		K521-a	EGINkSEkRMkLTLk
1	0		K524-a	NkSEkRMkLTLkGGA
0	2		K528-u	kRMkLTLkGGAAVDP
0	1		K548	HSAHVLEKGGkVFSA
0	13		K548-u	HSAHVLEkGGkVFSA
0	1		K551-u	HVLEkGGkVFSATLG
0	5		K564-u	LGLVDIVkGTNSYYK
0	2		K579-u	LQLLEDDkENRYWIF
0	1		K600-a	GTVIGSNkLEQMPsK
0	1		K600-u	GTVIGSNkLEQMPsK
0	1		S606-p	NkLEQMPsKEDAIEH
0	1		Y618	IEHFMKLYEEkTGNA
0	2		K621-a	FMKLYEEkTGNAWHS
0	1		K637-u	NFTKYPKkFYPLEID
0	1		T661-p	KLTVNPGtkSkLPkP
0	1		K662-u	LTVNPGtkSkLPkPV
0	1		K664-u	VNPGtkSkLPkPVQD
0	1		K667-u	GtkSkLPkPVQDLIk
0	1		K674-m2	kPVQDLIkMIFDVEs
0	1		S681-p	kMIFDVEsMKKAMVE
0	1		K695-u	EYEIDLQkMPLGKLS
0	5		K748-u	PHDFGMKkPPLLNNA
0	1		Y775-p	LLDIEVAySLLRGGs
1	12		S782-p	ySLLRGGsDDsskDP
1	0		S785-p	LRGGsDDsskDPIDV
1	0		S786-p	RGGsDDsskDPIDVN
0	3		K787-u	GGsDDsskDPIDVNY
0	5		K796-u	PIDVNYEkLkTDIKV
0	1		K798-u	DVNYEkLkTDIKVVD
0	1		S808-p	IKVVDRDsEEAEIIR
0	1		T824-p	YVKNTHAtTHNAYDL
0	1		K838-u	LEVIDIFkIEREGEC
0	3		K852-u	CQRYKPFkQLHNRRL
0	6		K940-u	KHASHISkLPKGKHS
0	7		K949-u	PKGKHSVkGLGKTTP
0	2		K1010-u	LKLKFNFkTSLW___

	mouse ► Hide Isoforms
S5	___MAEASERLYRVQ
S25	RASCKKCSESIPKDS
S27	SCKKCSESIPKDSLR
S32	SESIPKDSLRMAIMV
S41	RMAIMVQSPMFDGKV
K87	DDQQKVKKtAEAGGV
T88-p	DQQKVKKtAEAGGVA
K97	EAGGVAGKGQDGSGG
K97	EAGGVAGKGQDGSGG
K105-a	GQDGSGGkAEKTLGD
K108	GSGGkAEKTLGDFAA
K119	DFAAEYAKSNRSMCK
K131	MCKGCLEKIEKGQMR
K165	YHPTCFVKKRDELGF
S177	LGFRPEYSASQLKGF
S179	FRPEYSASQLKGFSL
K203	KKQLPAIKNEGKRKG
K209	IKNEGKRKGDEVDGT
K221	DGTDEVAKKKSRKET
S224	DEVAKKKSRKETDKY
K239	SKLEKALKAQNELIW
K249	NELIWNIKDELKKAC
K249	NELIWNIKDELKKAC
K253	WNIKDELKKACSTND
K254	NIKDELKKACSTNDL
K254	NIKDELKKACSTNDL
K262	ACSTNDLKELLIFNQ
Q269	KELLIFNQQQVPSGE
S274	FNQQQVPSGESAILD
K296-u	FGALLPCkECSGQLV
K320	GDVTAWTKCMVKTQN
K331	KTQNPSRKEWVTPKE
K337	RKEWVTPKEFREISY
H368	SSAPITVHWPLSVTS
S372	ITVHWPLSVTSAPTA
V373	TVHWPLSVTSAPTAV
S375	HWPLSVTSAPTAVNS
K394	DKPLSNMKILTLGKL
K400	MKILTLGKLSQNKDE
K400	MKILTLGKLSQNKDE
K414	EAKAVIEKLGGKLTG
K425	KLTGSANKASLCISI
K433	ASLCISIKKEVEKMN
K434	SLCISIKKEVEKMNK
K447	NKKMEEVKEANIRVV
K467	QDVSASTKSLQDLLS
K486	SPWGAEVKAEPGEVV
K486	SPWGAEVKAEPGEVV
K498	EVVAPRGKSAAPSKK
S499	VVAPRGKSAAPSKKS
K504	GKSAAPSKKSKGCFK
K507	AAPSKKSKGCFKEEG
K507	AAPSKKSKGCFKEEG
K517	FKEEGVNKSEKRMKL
K520	EGVNKSEKRMKLTLK
K523	NKSEKRMKLTLKGGA
K527	KRMKLTLKGGAAVDP
K547-a	HSAHVLEkGGKVFSA
K547-u	HSAHVLEkGGKVFSA
K550	HVLEkGGKVFSATLG
K563-u	LGLVDIVkGTNSYYK
K578-u	LQLLEDDkESRYWIF
K599	GTVIGSNKLEQMPSK
K599	GTVIGSNKLEQMPSK
S605	NKLEQMPSKEEAVEQ
Y617-p	VEQFMKLyEEKTGNA
K620	FMKLyEEKTGNAWHS
K636	NFTKYPKKFYPLEID
T660	KLTVKPGTKSKLPKP
K661	LTVKPGTKSKLPKPV
K663	VKPGTKSKLPKPVQE
K666	GTKSKLPKPVQELVG
G673	KPVQELVGMIFDVDS
S680	GMIFDVDSMKKALVE
K694	EYEIDLQKMPLGKLS
K747	PHDFGMKKPPLLNNA
Y774	LLDIEVAYSLLRGGs
S781-p	YSLLRGGsDDSSKDP
S784	LRGGsDDSSKDPIDV
S785	RGGsDDSSKDPIDVN
K786	GGsDDSSKDPIDVNY
K795	PIDVNYEKLKTDIKV
K797	DVNYEKLKTDIKVVD
S807	IKVVDRDSEEAEVIR
T823	YVKNTHATTHNAYDL
K837	LEVIDIFKIEREGES
R851	SQRYKPFRQLHNRRL
K939	KHASHISKLPKGKHS
K948	PKGKHSVKGLGKTTP
K1009	LKLKFNFKTSLW___

	PARP1 iso3
S5	___MAEASERLYRVE
S25	RASCKKCSESIPKDS
S27	SCKKCSESIPKDSLR
S32	SESIPKDSLRMTIMV
S41	RMTIMVQSPMFDGKV
K87	DDQQKVKKTAEAGGV
T88	DQQKVKKTAEAGGVA
K97	EAGGVAGKGQDGSGG
K97	EAGGVAGKGQDGSGG
K105	GQDGSGGKAEKTLGD
K108	GSGGKAEKTLGDFLA
K119-u	DFLAEYAkSNRSMCK
K131	MCKGCLEKIEKGQMR
K165	YHPTCFVKKRDELGF
S177	LGFRPEYSASQLKGF
S179	FRPEYSASQLKGFSL
K203	KKQLPAIKNEGKRKG
K209	IKNEGKRKGDEVDGT
K221	DGTDEVAKKKSKKGK
S224	DEVAKKKSKKGKDKD
K240	SKLEKALKAQNELIW
K250	NELIWNIKDELKKAC
K250	NELIWNIKDELKKAC
K254	WNIKDELKKACSTND
K255	NIKDELKKACSTNDL
K255	NIKDELKKACSTNDL
K263	ACSTNDLKELLIFNQ
Q270	KELLIFNQQQVPSGE
S275	FNQQQVPSGESAILD
K297	FGALLPCKECSGQLV
K321	GDVTAWTKCMVKTQN
K332	KTQNPSRKEWVTPKG
K338	RKEWVTPKGFREISY
A369	SSAPAPLALPLSVTS
S373	APLALPLSVTSAPTA
V374	PLALPLSVTSAPTAV
S376	ALPLSVTSAPTAVNS
K395	DKPLSNMKILTLGKL
K401	MKILTLGKLSQNKDE
K401	MKILTLGKLSQNKDE
K415	EAKAVIEKLGGKLTG
K426	KLTGSANKASLCIST
K434	ASLCISTKKEVEKMS
K435	SLCISTKKEVEKMSK
K448	SKKMEEVKAANVRVV
K468-u	QDVSASTkSLQELLS
K487	SSWGAEVKAEPGEVV
K487	SSWGAEVKAEPGEVV
K499	EVVAPKGKSAAPSKK
S500	VVAPKGKSAAPSKKS
K505	GKSAAPSKKSKGAVK
K508	AAPSKKSKGAVKEEG
K508	AAPSKKSKGAVKEEG
K518	VKEEGVNKSEkRMKL
K521-a	EGVNKSEkRMKLTLK
K524	NKSEkRMKLTLKGGA
K528	kRMKLTLKGGAAVDP
K548	HSAHVLEKGGKVFSA
K548	HSAHVLEKGGKVFSA
K551	HVLEKGGKVFSATLG
R564	LGLVDIVRGTNSYYK
K579	LQLLEDDKESRYWIF
K600	GTVIGSNKLEQMPSK
K600	GTVIGSNKLEQMPSK
S606	NKLEQMPSKEDAVEH
Y618	VEHFMKLYEEKTGNA
K621	FMKLYEEKTGNAWHS
K637	NFTKYPKKFYPLEID
T661	KLTVKPGTKSKLPKP
K662	LTVKPGTKSKLPKPV
K664	VKPGTKSKLPKPVQE
K667	GTKSKLPKPVQELVG
G674	KPVQELVGMIFDVES
S681	GMIFDVESMKKALVE
K695	EYEIDLQKMPLGKLS
K748	PHDFGMKKPPLLNNA
Y775	LLDIEVAYSLLRGGS
S782	YSLLRGGSDDSSKDP
S785	LRGGSDDSSKDPIDV
S786	RGGSDDSSKDPIDVN
K787	GGSDDSSKDPIDVNY
K796	PIDVNYEKLKTDIKV
K798	DVNYEKLKTDIKVVD
S808	IKVVDRDSEEAEVIR
T824	YVKNTHATTHNAYDL
K838	LEVIDIFKIEREGES
R852	SQRYKPFRQLHNRRL
K940	KHASHISKLPKGKHS
K949	PKGKHSVKGLGKTTP
K1010	LKLKFNFKTSLW___

	rat
T5	___MAEATERLYRVE
S25	RASCKKCSESIPKDS
S27	SCKKCSESIPKDSLR
S32	SESIPKDSLRMAIMV
S41	RMAIMVQSPMFDGKV
K87	DDQQKVKKTAEAGGV
T88	DQQKVKKTAEAGGVA
K97	EAGGVAGKGQHGGGG
K97	EAGGVAGKGQHGGGG
K105	GQHGGGGKAEKTLGD
K108	GGGGKAEKTLGDFAA
K119	DFAAEYAKSNRSTCK
K131	TCKGCMEKIEKGQMR
K165	YHPTCFVKNRDELGF
S177	LGFRPEYSASQLKGF
S179	FRPEYSASQLKGFSL
K203	KKQLPAVKSEGKRKC
K209	VKSEGKRKCDEVDGI
K221	DGIDEVAKKKSKKGK
S224	DEVAKKKSKKGKDKE
K240	SKLEKALKAQNELVW
K250	NELVWNIKDELKKAC
K250	NELVWNIKDELKKAC
K254	WNIKDELKKACSTND
K255	NIKDELKKACSTNDL
K255	NIKDELKKACSTNDL
K263	ACSTNDLKELLIFNQ
Q270	KELLIFNQQQVPSGE
S275	FNQQQVPSGESAILD
K297	FGALLPCKECSGQLV
K321	GDVTAWTKCMVKTQN
K332	KTQNPSRKEWVTPKE
K338	RKEWVTPKEFREISY
A369	SSAPAPPAPPVSITS
S373	APPAPPVSITSAPTA
I374	PPAPPVSITSAPTAV
S376	APPVSITSAPTAVNS
K395	DKPLSNMKILTLGKL
K401	MKILTLGKLSQNKDE
K401	MKILTLGKLSQNKDE
K415	EAKAMIEKLGGKLTG
K426	KLTGSANKASLCIST
K434	ASLCISTKKEVEKMS
K435	SLCISTKKEVEKMSK
K448	SKKMEEVKAANVRVV
K468	QDVSASAKSLQELLS
K487	SSWGAEVKVEPGEVV
K487	SSWGAEVKVEPGEVV
K499	EVVVPKGKSAAPSKK
S500	VVVPKGKSAAPSKKS
K505	GKSAAPSKKSKGAVK
K508	AAPSKKSKGAVKEEG
K508	AAPSKKSKGAVKEEG
K518	VKEEGVNKSEKRMKL
K521	EGVNKSEKRMKLTLK
K524	NKSEKRMKLTLKGGA
K528	KRMKLTLKGGAAVDP
K548	HSAHVLEKGGKVFSA
K548	HSAHVLEKGGKVFSA
K551	HVLEKGGKVFSATLG
K564	LGLVDIVKGTNSYYK
K579	LQLLESDKESRYWIF
K600	GTVIGSNKLEQMPSK
K600	GTVIGSNKLEQMPSK
S606	NKLEQMPSKEDAVEH
Y618	VEHFMKLYEEKTGNA
K621	FMKLYEEKTGNAWHS
K637	NFTKYPKKFYPLEID
T661	KLAVKPGTKSKLPKP
K662	LAVKPGTKSKLPKPV
K664	VKPGTKSKLPKPVQE
K667	GTKSKLPKPVQELVG
G674	KPVQELVGMIFDVES
S681	GMIFDVESMKKALVE
K695	EYEIDLQKMPLGKLS
K748	PHDFGMKKPPLLNNT
Y775	LLDIEVAYSLLRGGS
S782	YSLLRGGSDDSSKDP
S785	LRGGSDDSSKDPIDV
S786	RGGSDDSSKDPIDVN
K787	GGSDDSSKDPIDVNY
K796	PIDVNYEKLKTDIKV
K798	DVNYEKLKTDIKVVD
S808	IKVVDRDSEEAEVIR
T824	YVKNTHATTHNAYDL
K838	LEVIDIFKIEREGES
R852	SQRYKPFRQLHNRRL
K940	KHASHISKLPKGKHS
K949	PKGKHSVKGLGKTAP
K1010	LKLKFNFKTSLW___

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