Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Protein Page:
AP-2 alpha (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
AP-2 alpha Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region. Binds DNA as a dimer. Can form homodimers or heterodimers with other AP-2 family members. Interacts with WWOX. Interacts with CITED4. Interacts with UBE2I. Interacts with RALBP1 in a complex also containing EPN1 and NUMB during interphase and mitosis. Interacts with KCTD1; this interaction represses transcription activation. Interacts (via C-terminus) with CITED2 (via C-terminus); the interaction stimulates TFAP2A- transcriptional activation. Interacts (via N-terminus) with EP300 (via N-terminus); the interaction requires CITED2. Belongs to the AP-2 family. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: DNA binding protein; Transcription factor
Cellular Component: Golgi apparatus; centrosome; intracellular membrane-bound organelle; cytoplasm; nucleolus; nucleus
Molecular Function: protein dimerization activity; protein binding; protein homodimerization activity; sequence-specific DNA binding; transcription coactivator activity; chromatin binding
Biological Process: embryonic forelimb morphogenesis; transcription from RNA polymerase II promoter; negative regulation of epidermal growth factor receptor signaling pathway; neural crest cell development; regulation of neuron differentiation; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; palate development; embryonic pattern specification; negative regulation of cell proliferation; sympathetic nervous system development; sensory perception of sound; oculomotor nerve formation; epidermis morphogenesis; regulation of cell differentiation; positive regulation of neuron apoptosis; negative regulation of neuron apoptosis; kidney development; inner ear morphogenesis; trigeminal nerve development; anterior neuropore closure; embryonic cranial skeleton morphogenesis; positive regulation of bone mineralization; embryonic body morphogenesis; forebrain neuron development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; positive regulation of cell migration
Reference #:  P05549 (UniProtKB)
Alt. Names/Synonyms: Activating enhancer-binding protein 2-alpha; Activator protein 2; AP-2; AP-2 transcription factor; AP-2alpha; AP2-alpha; AP2A; AP2TF; BOFS; FLJ51761; TFAP2; TFAP2A; transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha); Transcription factor AP-2-alpha
Gene Symbols: TFAP2A
Molecular weight: 48,062 Da
Basal Isoelectric point: 8.1  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

AP-2 alpha

Protein Structure Not Found.


STRING  |  neXtProt  |  Protein Atlas  |  BioGPS  |  DISEASE  |  Scansite  |  Pfam  |  Phospho.ELM  |  NetworKIN  |  Source  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene


Sites Implicated In
transcription, altered: S258‑p
transcription, induced: S429‑p
molecular association, regulation: S258‑p
protein stabilization: S429‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 Y73-p YPQSQDPySHVNDPY
0 1 S185-p GPVSLSKsNsNAVSA
0 2 S187-p VSLSKsNsNAVSAIP
1 0 S219-p CSVPGRLsLLSSTSK
1 2 S239-p AEVQRRLsPPECLNA
2 0 S258-p GVLRRAKsKNGGRSL
0 1 R263 AKsKNGGRSLREKLD
1 0 S326-p EFLNRQHsDPNEQVT
0 1 S428-p HTDNNAKssDKEEKH
1 0 S429-p TDNNAKssDKEEKHR
  AP-2 alpha iso2  
Y67 YPQSQDPYSHVNDPY
S179 GPVSLSKSNSNAVSA
S181 VSLSKSNSNAVSAIP
S213 CSVPGRLSLLSSTSK
S233 AEVQRRLSPPECLNA
S252 GVLRRAKSKNGGRSL
R257 AKSKNGGRSLREKLD
S320 EFLNRQHSDPNEQVT
S422 HTDNNAKSSDKEEKH
S423 TDNNAKSSDKEEKHR
  mouse

 
Y73 YPQSQDPYSHVNDPY
S185 GPVSLSKSNSNAVSA
S187 VSLSKSNSNAVSAIP
S219 CSVPGRLSLLSSTSK
S239-p AEVQRRLsPPECLNA
S258-p GVLRRAKsKNGGrSL
R263-m1 AKsKNGGrSLREKLD
S326 EFLNRQHSDPNEQVA
S428 HTDNSAKSSDKEEKH
S429 TDNSAKSSDKEEKHR
Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.