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Protein Page:
AP-2 alpha (human)

Overview
AP-2 alpha Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region. Binds DNA as a dimer. Can form homodimers or heterodimers with other AP-2 family members. Interacts with WWOX. Interacts with CITED4. Interacts with UBE2I. Interacts with RALBP1 in a complex also containing EPN1 and NUMB during interphase and mitosis. Interacts with KCTD1; this interaction represses transcription activation. Interacts (via C-terminus) with CITED2 (via C-terminus); the interaction stimulates TFAP2A- transcriptional activation. Interacts (via N-terminus) with EP300 (via N-terminus); the interaction requires CITED2. Belongs to the AP-2 family. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Transcription factor; DNA binding protein
Cellular Component: Golgi apparatus; centrosome; intracellular membrane-bound organelle; cytoplasm; nucleolus; nucleus
Molecular Function: protein dimerization activity; protein binding; protein homodimerization activity; sequence-specific DNA binding; transcription coactivator activity; chromatin binding
Biological Process: transcription from RNA polymerase II promoter; embryonic forelimb morphogenesis; negative regulation of epidermal growth factor receptor signaling pathway; neural crest cell development; regulation of neuron differentiation; positive regulation of transcription, DNA-dependent; palate development; negative regulation of transcription from RNA polymerase II promoter; embryonic pattern specification; negative regulation of cell proliferation; sympathetic nervous system development; sensory perception of sound; oculomotor nerve formation; regulation of cell differentiation; positive regulation of neuron apoptosis; epidermis morphogenesis; negative regulation of neuron apoptosis; kidney development; inner ear morphogenesis; trigeminal nerve development; anterior neuropore closure; embryonic cranial skeleton morphogenesis; positive regulation of bone mineralization; embryonic body morphogenesis; forebrain neuron development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of cell migration; negative regulation of apoptosis
Reference #:  P05549 (UniProtKB)
Alt. Names/Synonyms: Activating enhancer-binding protein 2-alpha; Activator protein 2; AP-2; AP-2 transcription factor; AP-2alpha; AP2-alpha; AP2A; AP2TF; BOFS; FLJ51761; TFAP2; TFAP2A; transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha); Transcription factor AP-2-alpha
Gene Symbols: TFAP2A
Molecular weight: 48,062 Da
Basal Isoelectric point: 8.1  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

AP-2 alpha

Protein Structure Not Found.


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Sites Implicated In
transcription, altered: S258‑p
transcription, induced: S429‑p
molecular association, regulation: S258‑p
protein stabilization: S429‑p

Modification Sites and Domains  

Modification Sites in Parent Protein, Orthologs, and Isoforms  
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 S185-p GPVSLSKsNsNAVSA
0 2 S187-p VSLSKsNsNAVSAIP
1 0 S219-p CSVPGRLsLLSSTSK
1 2 S239-p AEVQRRLsPPECLNA
2 0 S258-p GVLRRAKsKNGGRSL
1 0 S326-p EFLNRQHsDPNEQVT
1 0 S429-p TDNNAKSsDKEEKHR
  AP-2 alpha iso2  
S179 GPVSLSKSNSNAVSA
S181 VSLSKSNSNAVSAIP
S213 CSVPGRLSLLSSTSK
S233 AEVQRRLSPPECLNA
S252 GVLRRAKSKNGGRSL
S320 EFLNRQHSDPNEQVT
S423 TDNNAKSSDKEEKHR
  mouse

 
S185 GPVSLSKSNSNAVSA
S187 VSLSKSNSNAVSAIP
S219 CSVPGRLSLLSSTSK
S239-p AEVQRRLsPPECLNA
S258-p GVLRRAKsKNGGRSL
S326 EFLNRQHSDPNEQVA
S429 TDNSAKSSDKEEKHR
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