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Protein Page:
PCAF (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PCAF Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Interacts with SIRT1. Interacts (unsumoylated form) with NR2C1; the interaction promotes transactivation activity. Interacts with EP300, CREBBP and DDX17. Interacts with NCOA1 and NCOA3. Component of a large chromatin remodeling complex, at least composed of MYSM1, KAT2B/PCAF, RBM10 and KIF11/TRIP5. Interacts with NR2C2 (hypophosphorylated and unsumoylated form); the interaction promotes the transactivation activity of NR2C2. Binds to HTLV-1 Tax. Interacts with and acetylates HIV-1 Tat. Interacts with KLF1; the interaction does not acetylate KLF1 and there is no enhancement of its transactivational activity. Interacts with NFE4. Interacts with MECOM. Interacts with E2F1; the interaction acetylates E2F1 augmenting its DNA-binding and transcriptional activity. Ubiquitously expressed but most abundant in heart and skeletal muscle. Belongs to the GCN5 family. Note: This description may include information from UniProtKB.
Protein type: Nuclear receptor co-regulator; Acetyltransferase; EC 2.3.1.48
Cellular Component: nucleoplasm; kinetochore; I band; PCAF complex; actomyosin; nucleus; A band
Molecular Function: cyclin-dependent protein kinase inhibitor activity; lysine N-acetyltransferase activity; protein binding; histone acetyltransferase activity; histone deacetylase binding; transcription coactivator activity; acetyltransferase activity; protein complex binding; transcription cofactor activity; transcription factor binding; protein kinase binding
Biological Process: transcription initiation from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; Notch signaling pathway; internal peptidyl-lysine acetylation; viral reproduction; rhythmic process; negative regulation of cyclin-dependent protein kinase activity; transcription from RNA polymerase I promoter; chromatin remodeling; negative regulation of cell proliferation; protein amino acid acetylation; cellular response to insulin stimulus; gene expression; positive regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase I promoter; cell cycle arrest; peptidyl-lysine acetylation; N-terminal peptidyl-lysine acetylation
Reference #:  Q92831 (UniProtKB)
Alt. Names/Synonyms: CAF; CREBBP-associated factor; Histone acetylase PCAF; Histone acetyltransferase KAT2B; Histone acetyltransferase PCAF; K(lysine) acetyltransferase 2B; KAT2B; Lysine acetyltransferase 2B; P; P/CAF; P300/CBP-associated factor; PCAF
Gene Symbols: KAT2B
Molecular weight: 93,013 Da
Basal Isoelectric point: 9.16  Predict pI for various phosphorylation states
CST Pathways:  Crosstalk between PTMs  |  G2/M DNA Damage Checkpoint  |  NF-kB Signaling  |  Protein Acetylation
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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PCAF

Protein Structure Not Found.


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Sites Implicated In
enzymatic activity, induced: K416‑ac, K428‑ac, K430‑ac, K441‑ac, K442‑ac

Modification Sites and Domains Show Modification Legend
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Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S40 PPAPPQGSPCAAAAG
0 1 S49-p CAAAAGGsGACGPAT
0 1 S72-p EGPGGGGsARIAVkk
0 1 K78-ac GsARIAVkkAQLRSA
1 0 K78-me GsARIAVkkAQLRSA
1 0 K78-m1 GsARIAVkkAQLRSA
0 1 K79-ac sARIAVkkAQLRSAP
1 0 K89-me LRSAPRAkKLEKLGV
1 0 K89-m1 LRSAPRAkKLEKLGV
0 1 T119-p KNPNPSPtPPRADLQ
0 1 S270-p PSQRRLRsPNDDIsG
0 1 S276-p RsPNDDIsGYKENYT
0 1 Y301-p FCDSLPRyETTQVFG
0 1 T310-p TTQVFGRtLLRsVFt
0 1 S314-p FGRtLLRsVFtVMRR
0 1 T317-p tLLRsVFtVMRRQLL
1 0 K416-ac GSSSPACkASSGLEA
1 0 K428-ac LEANPGEkRkMTDSH
1 0 K430-ac ANPGEkRkMTDSHVL
1 0 K441-ac SHVLEEAkkPRVMGD
1 0 K442-ac HVLEEAkkPRVMGDI
0 1 Y533-p LPRMPKEyITRLVFD
1 0 K638-me TKYVGYIkDYEGATL
1 0 K638-m1 TKYVGYIkDYEGATL
0 1 K638-ub TKYVGYIkDYEGATL
1 0 K671-me KKQKEIIkkLIERKQ
1 0 K672-me KQKEIIkkLIERKQA
1 0 K692-me YPGLSCFkDGVRQIP
0 40 Y729-p PRDPDQLyStLkSIL
0 2 T731-p DPDQLyStLkSILQQ
0 1 K733-ac DQLyStLkSILQQVK
0 1 Y802-p VFTNCKEyNPPEsEy
0 1 S807-p KEyNPPEsEyyKCAN
0 1 Y809-p yNPPEsEyyKCANIL
0 1 Y810-p NPPEsEyyKCANILE
  mouse

 
S20-p PPAPPHGsPRTLATA
S31 LATAAGSSASCGPAT
S54 EGPGGGGSARIAVKK
K60 GSARIAVKKAQLRSA
K60 GSARIAVKKAQLRSA
K60 GSARIAVKKAQLRSA
K61 SARIAVKKAQLRSAP
K71 LRSAPRAKKLEKLGV
K71 LRSAPRAKKLEKLGV
T101-p KNPNPSPtPPRGDLQ
S252 PSQRRLRSPNDDISG
S258 RSPNDDISGYKENYT
Y283 FCDSLPRYETTKVFG
T292 TTKVFGRTLLRSVFT
S296 FGRTLLRSVFTIMRR
T299 TLLRSVFTIMRRQLL
R397 GRTSPGCRGSSGLEA
K409 LEANPGEKRKMNNSH
K411 ANPGEKRKMNNSHAP
K422 SHAPEEAKRSRVMGD
R423 HAPEEAKRSRVMGDI
Y514 LPRMPKEYITRLVFD
K619 TKYVGYIKDYEGATL
K619 TKYVGYIKDYEGATL
K619 TKYVGYIKDYEGATL
K652 KKQKEIIKKLIERKQ
K653 KQKEIIKKLIERKQA
K673 YPGLSCFKDGVRQIP
Y710-p PKDPEQLyStLKNIL
T712-p DPEQLyStLKNILQQ
K714 EQLyStLKNILQQVK
Y783 VFTNCKEYNPPESEY
S788 KEYNPPESEYYKCAS
Y790 YNPPESEYYKCASIL
Y791 NPPESEYYKCASILE
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