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Protein Page:
MIB1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
MIB1 E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF- induced apoptosis. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation. Widely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined. Note: This description may include information from UniProtKB.
Protein type: EC 6.3.2.19; Ligase; EC 6.3.2.-; Ubiquitin ligase; Ubiquitin conjugating system
Cellular Component: centrosome; postsynaptic density; plasma membrane; cytoplasmic vesicle; cytosol
Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity
Biological Process: blood vessel development; negative regulation of neuron differentiation; Notch signaling pathway; somitogenesis; in utero embryonic development; protein ubiquitination; positive regulation of endocytosis; heart looping; neural tube formation
Reference #:  Q86YT6 (UniProtKB)
Alt. Names/Synonyms: DAPK-interacting protein 1; DIP-1; DIP1; DKFZp686I0769; DKFZp761M1710; E3 ubiquitin-protein ligase MIB1; FLJ90676; KIAA1323; MGC129659; MGC129660; MIB; MIB1; Mind bomb homolog 1; mindbomb homolog 1 (Drosophila); ubiquitin ligase mind bomb; ubiquitin ligase protein MIB1; Zinc finger ZZ type with ankyrin repeat domain protein 2; ZZANK2; ZZZ6
Gene Symbols: MIB1
Molecular weight: 110,136 Da
Basal Isoelectric point: 6.47  Predict pI for various phosphorylation states
CST Pathways:  Notch Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

MIB1

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 80 K28-ub GPDWKWGkQDGGEGH
0 2 K79-ub DSAPTGIkHDGTMCD
0 1 Y106-p KCAECTNyDLCTVCY
0 3 Y194-p SSPHSAAyVLWDNGA
0 1 K202-ac VLWDNGAkNLYRVGF
0 11 K202-ub VLWDNGAkNLYRVGF
0 1 K216-ac FEGMSDLkCVQDAkG
0 14 K222-ub LkCVQDAkGGSFYRD
0 1 K313-ub FNPAVLTkANIVRSG
0 4 K368-ub AMLPTLGkVGRVQQI
0 1 K399-ub YNPAAVSkVAsAGsA
0 1 S402-p AAVSkVAsAGsAIsN
0 1 S405-p SkVAsAGsAIsNAsG
0 3 S408-p AsAGsAIsNAsGERL
0 1 S411-p GsAIsNAsGERLsQL
0 16 S416-p NAsGERLsQLLKkLF
0 41 K421-ub RLsQLLKkLFETQES
0 12 K437-ub DLNEELVkAAANGDV
0 1 K481-ub NGHVDILkLLLkQNV
0 50 K485-ub DILkLLLkQNVDVEA
0 5 K495-ub VDVEAEDkDGDRAVH
0 2 K618-ub AMRVLLSkLPRPWIV
0 2 K691-ub LLVRAGAkLDIQDKD
0 16 K726-ub QDMQDVGkVDAAWEP
0 4 K735-ub DAAWEPSkNTLIMGL
0 6 K749-ub LGTQGAEkkSAASIA
0 1 K750-ub GTQGAEkkSAASIAC
0 1 S767-p AANGADLsIRNKkGQ
0 25 K772-ub DLsIRNKkGQSPLDL
0 28 K787-ub CPDPNLCkALAkCHK
0 4 K791-ub NLCkALAkCHKEkVS
0 4 K796-ub LAkCHKEkVSGQVGS
0 1 S805 SGQVGSRSPSMISND
0 1 K826-ub CMVCSDMkRDTLFGP
0 10 K854-ub VKKCLICkEQVQSRT
0 8 K872-ub ECVVCSDkkAAVLFQ
0 14 K873-ub CVVCSDkkAAVLFQP
0 1 K895-ub ENCANLMkkCVQCRA
0 2 K896-ub NCANLMkkCVQCRAV
0 1 K917-ub FIMCCGGkSSEDATD
0 29 K936-ub GNIPVLQkDkDNTNV
0 46 K938-ub IPVLQkDkDNTNVNA
0 22 K949-ub NVNADVQkLQQQLQD
0 1 K958-ub QQQLQDIkEQTMCPV
  mouse

 
K28-ub GPDWKWGkQDGGEGH
K79 DSAPTGIKHDGTMCD
Y106 KCAECTNYDLCTVCY
Y194 SSPHSAAYVLWDNGA
K202 VLWDNGAKNLYRVGF
K202 VLWDNGAKNLYRVGF
K216 FEGMSDLKCVQDAkG
K222-ub LKCVQDAkGGSFYRD
K313 FNPAVLTKANIVRSG
K368 AMLPTLGKVGRVQQI
K399 YNPAAVSKVAPAGSA
P402 AAVSKVAPAGSAIsN
S405 SKVAPAGSAIsNASG
S408-p APAGSAIsNASGERL
S411 GSAIsNASGERLsQL
S416-p NASGERLsQLLKkLF
K421-ub RLsQLLKkLFETQES
K437 DLNEELVKAAANGDV
K481 NGHVDILKLLLkQNV
K485-ub DILKLLLkQNVDVEA
K495 VDVEAEDKDGDRAVH
K618 AMRVLLSKLPRPWIV
K691 LLVRAGAKLDIQDKD
K726-ub QDMQDVGkVDAAWEP
K735 DAAWEPSKNTLIMGL
K749 LGTQGAEKKSAASIA
K750 GTQGAEKKSAASIAC
S767 AANGADLSIRNKKGQ
K772 DLSIRNKKGQSPLDL
K787 CPDPSLCKALAKCHK
K791 SLCKALAKCHKEKVS
K796 LAKCHKEKVSGQVGS
S805-p SGQVGSRsPSMISND
K826 CMVCSDMKRDTLFGP
K854 VKKCLICKEQVQSRT
K872 ECVVCSDKKAAVLFQ
K873 CVVCSDKKAAVLFQP
K895 ENCASLMKKCVQCRA
K896 NCASLMKKCVQCRAV
K917 FITCCGGKSSEDPSD
K936-ub GNIPVLQkDkDNTNV
K938-ub IPVLQkDkDNTNVNA
K949-ub NVNADVQkLQQQLQD
K958 QQQLQDIKEQTMCPV
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