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Protein Page:
PIAS4 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PIAS4 Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Promotes PARK7 sumoylation. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Interacts with AR, AXIN1, GATA2, LEF1, TP53 and STAT1 (IFNG-induced). Binds to AT-rich DNA sequences, known as matrix or scaffold attachment regions (MARs/SARs). Interacts with TICAM1. Interacts with KLF8; the interaction results in SUMO ligation and repression of KLF8 transcriptional activity and of its cell cycle progression into G(1) phase. Highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes. Belongs to the PIAS family. Note: This description may include information from UniProtKB.
Protein type: Transcription, coactivator/corepressor; EC 6.3.2.-; Nuclear receptor co-regulator; SUMO conjugating system
Cellular Component: PML body; nuclear matrix; cytoplasm; nucleolus; nucleus
Molecular Function: protein binding; DNA binding; zinc ion binding; ubiquitin protein ligase binding; SUMO ligase activity
Biological Process: positive regulation of protein sumoylation; Wnt receptor signaling pathway; protein sumoylation; transcription, DNA-dependent; inhibition of NF-kappaB transcription factor; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent
Reference #:  Q8N2W9 (UniProtKB)
Alt. Names/Synonyms: E3 SUMO-protein ligase PIAS4; FLJ12419; MGC35296; PIAS-gamma; PIAS4; PIASG; PIASy; Protein inhibitor of activated STAT protein 4; Protein inhibitor of activated STAT protein gamma; protein inhibitor of activated STAT protein PIASy; protein inhibitor of activated STAT, 4; zinc finger, MIZ-type containing 6; ZMIZ6
Gene Symbols: PIAS4
Molecular weight: 56,504 Da
Basal Isoelectric point: 5.84  Predict pI for various phosphorylation states
CST Pathways:  IL6 Signaling  |  NF-kB Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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PIAS4

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
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Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

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 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 K31 LGFVGRSKSGLkHEL
1 0 K35-sm GRSKSGLkHELVTRA
0 3 T99-p RAGAVPRtPLAGPNI
0 3 Y108-p LAGPNIDyPVLYGkY
0 1 K114-ac DyPVLYGkYLNGLGR
0 1 K114-ub DyPVLYGkYLNGLGR
0 1 K125-ac GLGRLPAkTLKPEVR
  mouse

 
K31-ub LGFVGRSkSGLKHEL
K35 GRSkSGLKHELVTRA
T92 ALHSMPRTPLSGPTV
Y101 LSGPTVDYPVLYGKY
K107 DYPVLYGKYLNGLGR
K107 DYPVLYGKYLNGLGR
K118 GLGRLPTKTLKPEVR
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