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Protein Page:
Fli1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
Fli1 Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'. Defects in FLI1 are a cause of Ewing sarcoma (ES). A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1. Belongs to the ETS family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Transcription factor; DNA binding protein
Chromosomal Location of Human Ortholog: 11q24.1-q24.3
Cellular Component: nucleus
Molecular Function: DNA binding; sequence-specific DNA binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; organ morphogenesis; hemostasis; cell differentiation
Reference #:  Q01543 (UniProtKB)
Alt. Names/Synonyms: EWSR2; FLI1; Friend leukemia integration 1 transcription factor; Friend leukemia virus integration 1; Proto-oncogene Fli-1; SIC-1; Transcription factor ERGB
Gene Symbols: FLI1
Molecular weight: 50,982 Da
Basal Isoelectric point: 6.57  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Fli1

Protein Structure Not Found.


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Sites Implicated In
transcription, altered: T312‑p
transcription, induced: T312‑p
acetylation: T312‑p
molecular association, regulation: T312‑p
protein degradation: T312‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
1 1 S10 GTIKEALSVVSDDQS
1 1 S13 KEALSVVSDDQSLFd
0 1 D20-ca SDDQSLFdSAYGAAA
1 2 T35-p HLPKADMtAsGsPDy
1 2 S37-p PKADMtAsGsPDyGQ
1 13 S39-p ADMtAsGsPDyGQPH
1 25 Y42-p tAsGsPDyGQPHKIN
1 0 K67-sm QPVRVNVkREyDHMN
0 1 Y70-p RVNVkREyDHMNGsR
0 1 S76-p EyDHMNGsRESPVDC
1 1 S79 HMNGsRESPVDCSVs
0 1 S86-p SPVDCSVsKCsKLVG
0 1 S89-p DCSVsKCsKLVGGGE
0 2 K172-ub KELCKMNkEDFLRAT
0 7 S221-p LSVKEDPsyDsVRRG
0 103 Y222-p SVKEDPsyDsVRRGA
0 10 S224-p KEDPsyDsVRRGAWG
0 3 S236-p AWGNNMNsGLNksPP
1 0 K240-ac NMNsGLNksPPLGGA
1 26 S241-p MNsGLNksPPLGGAQ
1 0 K252-ac GGAQTISkNTEQRPQ
0 6 Y263-p QRPQPDPyQILGPTS
5 0 T312-p TNGEFKMtDPDEVAR
0 1 Y332-p KSKPNMNyDKLSRAL
3 0 K380-ac PTESSMYkYPSDISY
1 0 K397-ac SYHAHQQkVNFVPPH
0 6 S450-p HVPSHLGsyy_____
0 47 Y451-p VPSHLGsyy______
0 6 Y452-p PSHLGsyy_______
  mouse

 
S10-p GTIKEALsVVsDDQS
S13-p KEALsVVsDDQSLFD
D20 sDDQSLFDSAYGAAA
T35-p HLPKADMtAsGsPDy
S37-p PKADMtAsGsPDyGQ
S39-p ADMtAsGsPDyGQPH
Y42-p tAsGsPDyGQPHKIN
K67 QPVRVNVKREYDHMN
Y70 RVNVKREYDHMNGSR
S76 EYDHMNGSREsPVDC
S79-p HMNGSREsPVDCSVS
S86 sPVDCSVSKCNKLVG
N89 DCSVSKCNKLVGGGE
K172 KELCKMNKEDFLRAT
S221 LNVKEDPSYDSVRRG
Y222 NVKEDPSYDSVRRGA
S224 KEDPSYDSVRRGAWN
S236-p AWNNNMNsGLNKsPL
K240 NMNsGLNKsPLLGGS
S241-p MNsGLNKsPLLGGSQ
K252 GGSQTMGKNTEQRPQ
Y263 QRPQPDPYQILGPTS
T312 TNGEFKMTDPDEVAR
Y332 KSKPNMNYDKLSRAL
K380 PTETSMYKYPSDISY
K397 SYHAHQQKVNFVPSH
S450 HVPSHLGSYY_____
Y451 VPSHLGSYY______
Y452 PSHLGSYY_______
  rat

 
S10 GTIKEALSVVSDDQS
S13 KEALSVVSDDQSLFD
D20 SDDQSLFDSAYGAAA
T35 HLPKADMTASGsPDY
S37 PKADMTASGsPDYGQ
S39-p ADMTASGsPDYGQPH
Y42 TASGsPDYGQPHKIN
K67 QPVRVNVKREYDHMN
Y70 RVNVKREYDHMNGSR
S76 EYDHMNGSRESPVDC
S79 HMNGSRESPVDCSVS
S86 SPVDCSVSKCNKLVG
N89 DCSVSKCNKLVGGGE
K172 KELCKMSKEDFLRAT
S221 LNVKEDPSYDSVRRG
Y222 NVKEDPSYDSVRRGG
S224 KEDPSYDSVRRGGWS
S236 GWSNNMNSGINKsPP
K240 NMNSGINKsPPLGGA
S241-p MNSGINKsPPLGGAQ
K252 GGAQSMGKNTEQRPQ
Y263 QRPQPDPYQILGPTS
T312 TNGEFKMTDPDEVAR
Y332 KSKPNMNYDKLSRAL
K380 PTETSMYKYPSDISY
K397 SYHAHQQKVNFVQPH
S450 HVPSHLGSYY_____
Y451 VPSHLGSYY______
Y452 PSHLGSYY_______
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