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Protein Page:
RIP3 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
g O-GlcNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
RIP3 Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F- actin-binding region leads to disassembly of stress fibers in neuronal cells. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: GTPase activating protein, Rac/Rho
Cellular Component: cytoskeleton; cytoplasm
Molecular Function: phospholipid binding; actin binding
Reference #:  Q6WCQ1 (UniProtKB)
Alt. Names/Synonyms: KIAA0864; M-RIP; MPRIP; MRIP; myosin phosphatase Rho interacting protein; Myosin phosphatase Rho-interacting protein; myosin phosphatase-Rho interacting protein; p116Rip; Rho interacting protein 3; Rho-interacting protein 3; RHOIP3; RIP3
Gene Symbols: MPRIP
Molecular weight: 116,533 Da
Basal Isoelectric point: 5.89  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

RIP3
Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


  MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


        human

► Hide Isoforms 		 
0 2 Y148 WLEMLMVYPRTNKQN
0 1 S180 AKVAVTSSSSSSSSS
0 1 S187 SSSSSSSSSSIPsAE
0 1 S189 SSSSSSSSIPsAEKV
0 1 - gap
0 1 - gap
0 4 S192-p SSSSSIPsAEKVPTT
0 1 S201 EKVPTTKSTLWQEEM
0 1 T202 KVPTTKSTLWQEEMR
0 2 S217 TKDQPDGSsLsPAQs
0 3 S218-p KDQPDGSsLsPAQsP
0 8 S220-p QPDGSsLsPAQsPsQ
0 8 S224-p SsLsPAQsPsQSQPP
0 5 S226-p LsPAQsPsQSQPPAA
0 1 S228 PAQsPsQSQPPAASS
0 1 S251 KEEESAMSSDRMDCG
0 13 S265-p GRKVRVEsGyFsLEK
0 88 Y267-p KVRVEsGyFsLEKTK
0 15 S269-p RVEsGyFsLEKTKQD
0 31 S289-p QQLPPPLsPPsPstP
0 32 S292-p PPPLsPPsPstPNHR
0 10 S294-p PLsPPsPstPNHRRs
0 22 T295-p LsPPsPstPNHRRsQ
0 2 H298 PsPstPNHRRsQVIE
0 2 S301-p stPNHRRsQVIEKFE
0 1 T320 EKAEHMETNAVGPsP
0 2 S326-p ETNAVGPsPSSDTRQ
0 1 K344 EKRAFPRKRDFtNEA
0 1 T348-p FPRKRDFtNEAPPAP
0 1 P353 DFtNEAPPAPLPDAs
0 1 S360-p PAPLPDAsAsPLsPH
0 3 S362-p PLPDAsAsPLsPHRR
0 11 S365-p DAsAsPLsPHRRAKs
0 3 S372-p sPHRRAKsLDRRstE
0 6 S377-p AKsLDRRstEPSVTP
0 8 T378-p KsLDRRstEPSVTPD
0 1 P380 LDRRstEPSVTPDLL
0 1 S381 DRRstEPSVTPDLLN
0 1 K390 TPDLLNFKKGWLTkQ
0 1 K391 PDLLNFKKGWLTkQY
0 11 K396-u FKKGWLTkQYEDGQW
0 5 Y450-p EYPVQRNyGFQIHTK
0 3 T486 MKHVHPTTAPDVTSS
0 1 S504 EKNKSSCSFETCPRP
0 1 K527-u GEPDPEQkRSRARER
0 7 S540-p ERRREGRsKtFDWAE
0 176 T542-p RREGRsKtFDWAEFR
0 1 A601 KRFGMLDAtDGPGTE
0 1 T602-p RFGMLDAtDGPGTED
0 10 S619-p LRMEVDRsPGLPMsD
0 2 M624 DRsPGLPMsDLKTHN
0 1 S625-p RsPGLPMsDLKTHNV
0 2 S662 PIAPVHLSSEDGGDR
0 2 S671 EDGGDRLSTHELTSL
0 1 T672 DGGDRLSTHELTSLL
0 1 S691-p EQSQKEAsDLLEQNR
0 3 S713-p VALGREQsAREGyVL
0 1 Y718-p EQsAREGyVLQATCE
0 2 - gap
0 3 - gap
0 1 - gap
0 1 - gap
0 1 - gap
0 1 - gap
0 1 - gap
0 1 S815-p QYLEELQsVQRELEV
0 5 S891-p GGGEATGsPLAQGKD
0 1 Y915-p VKESEIQyLKQEIsS
0 1 S921-p QyLKQEIsSLKDELQ
0 42 Y945-p SDKYKDIytELSIAK
0 1 T946-p DKYKDIytELSIAKA
0 1 T970-p KEQLKAAtEALGEks
0 1 K976-u AtEALGEksPDSATV
0 9 S977-p tEALGEksPDSATVs
0 1 S984-p sPDSATVsGyDIMKs
0 15 Y986-p DSATVsGyDIMKsKs
0 4 S991-p sGyDIMKsKsNPDFL
0 15 S993-p yDIMKsKsNPDFLKK
0 11 S1014-p RQLRNIRsKsVIEQV
0 29 S1016-p LRNIRsKsVIEQVSW
0 1 S1022 KsVIEQVSWDT____
  RIP3 iso2  
Y148 WLEMLMVYPRTNKQN
S180 AKVAVTSSSSSSSSS
S187 SSSSSSSSSSIPsAE
S189 SSSSSSSSIPsAEKV
- gap
- gap
S192-p SSSSSIPsAEKVPTT
S201 EKVPTTKSTLWQEEM
T202 KVPTTKSTLWQEEMR
S217 TKDQPDGSSLSPAQS
S218 KDQPDGSSLSPAQSP
S220 QPDGSSLSPAQSPSQ
S224 SSLSPAQSPSQSQPP
S226 LSPAQSPSQSQPPAA
S228 PAQSPSQSQPPAASS
S251 KEEESAMSSDRMDCG
S265 GRKVRVESGYFSLEK
Y267 KVRVESGYFSLEKTK
S269 RVESGYFSLEKTKQD
S289 QQLPPPLSPPSPSTP
S292 PPPLSPPSPSTPNHR
S294 PLSPPSPSTPNHRRS
T295 LSPPSPSTPNHRRSQ
H298 PSPSTPNHRRSQVIE
S301 STPNHRRSQVIEKFE
T320 EKAEHMETNAVGPSP
S326 ETNAVGPSPSSDTRQ
K344 EKRAFPRKRDFTNEA
T348 FPRKRDFTNEAPPAP
P353 DFTNEAPPAPLPDAS
S360 PAPLPDASASPLSPH
S362 PLPDASASPLSPHRR
S365 DASASPLSPHRRAKS
S372 SPHRRAKSLDRRSTE
S377 AKSLDRRSTEPSVTP
T378 KSLDRRSTEPSVTPD
P380 LDRRSTEPSVTPDLL
S381 DRRSTEPSVTPDLLN
K390 TPDLLNFKKGWLTKQ
K391 PDLLNFKKGWLTKQY
K396 FKKGWLTKQYEDGQW
Y450 EYPVQRNYGFQIHTK
T486 MKHVHPTTAPDVTSS
S504 EKNKSSCSFETCPRP
K527 GEPDPEQKRSRARER
S540 ERRREGRSKTFDWAE
T542 RREGRSKTFDWAEFR
A601 KRFGMLDATDGPGTE
T602 RFGMLDATDGPGTED
S619 LRMEVDRSPGLPMSD
M624 DRSPGLPMSDLKTHN
S625 RSPGLPMSDLKTHNV
S662 PIAPVHLSSEDGGDR
S671 EDGGDRLSTHELTSL
T672 DGGDRLSTHELTSLL
S691 EQSQKEASDLLEQNR
S713 VALGREQSAREGYVL
Y718 EQSAREGYVLQATCE
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S815 QYLEELQSVQRELEV
S891 GGGEATGSPLAQGKD
Y915 VKESEIQYLKQEISS
S921 QYLKQEISSLKDELQ
Y945 SDKYKDIYTELSIAK
T946 DKYKDIYTELSIAKA
T970 KEQLKAATEALGEKS
K976 ATEALGEKSPDSATV
S977 TEALGEKSPDSATVS
S984 SPDSATVSGYDIMKS
Y986 DSATVSGYDIMKSKS
S991 SGYDIMKSKSNPDFL
S993 YDIMKSKSNPDFLKK
S1014-p RQLRNIRsKsLKEGL
S1016-p LRNIRsKsLKEGLTV
- gap
  RIP3 iso3  
Y148 WLEMLMVYPRTNKQN
S180 AKVAVTSSSSSSSSS
S187 SSSSSSSSSSIPSAE
S189 SSSSSSSSIPSAEKV
- gap
- gap
S192 SSSSSIPSAEKVPTT
S201 EKVPTTKSTLWQEEM
T202 KVPTTKSTLWQEEMR
S217 TKDQPDGSSLSPAQS
S218 KDQPDGSSLSPAQSP
S220 QPDGSSLSPAQSPSQ
S224 SSLSPAQSPSQSQPP
S226 LSPAQSPSQSQPPAA
S228 PAQSPSQSQPPAASS
S251 KEEESAMSSDRMDCG
S265 GRKVRVESGYFSLEK
Y267 KVRVESGYFSLEKTK
S269 RVESGYFSLEKTKQD
S289 QQLPPPLSPPSPSTP
S292 PPPLSPPSPSTPNHR
S294 PLSPPSPSTPNHRRS
T295 LSPPSPSTPNHRRSQ
H298 PSPSTPNHRRSQVIE
S301 STPNHRRSQVIEKFE
T320 EKAEHMETNAVGPSP
S326 ETNAVGPSPSSDTRQ
K344-u EKRAFPRkRPDLLNF
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
K352-u RPDLLNFkkGWLTKQ
K353-u PDLLNFkkGWLTKQY
K358 FkkGWLTKQYEDGQW
Y412 EYPVQRNYGFQIHTK
T448 MKHVHPTTAPDVTSS
S466 EKNKSSCSFETCPRP
K489 GEPDPEQKRSRARER
S502 ERRREGRSKTFDWAE
T504 RREGRSKTFDWAEFR
A563 KRFGMLDATDGPGTE
T564 RFGMLDATDGPGTED
S581 LRMEVDRSPGLPMSD
M586 DRSPGLPMSDLKTHN
S587 RSPGLPMSDLKTHNV
S624 PIAPVHLSSEDGGDR
S633 EDGGDRLSTHELTSL
T634 DGGDRLSTHELTSLL
S653 EQSQKEASDLLEQNR
S675 VALGREQSAREGYVL
Y680 EQSAREGYVLQATCE
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S777 QYLEELQSVQRELEV
S853 GGGEATGSPLAQGKD
Y877 VKESEIQYLKQEISS
S883 QYLKQEISSLKDELQ
Y907 SDKYKDIYTELSIAK
T908 DKYKDIYTELSIAKA
T932 KEQLKAATEALGEKS
K938 ATEALGEKSPDSATV
S939 TEALGEKSPDSATVS
S946 SPDSATVSGYDIMKS
Y948 DSATVSGYDIMKSKS
S953 SGYDIMKSKSNPDFL
S955 YDIMKSKSNPDFLKK
S976-p RQLRNIRsKsLKEGL
S978-p LRNIRsKsLKEGLTV
- gap
  mouse

► Hide Isoforms 		 
Y148 WLEMLMVYPRTNKQN
S180 AKMAVTSSSGGSSGS
S188 SGGSSGSSSSIPSAE
S190 GSSGSSSSIPSAEKV
- gap
- gap
S193 GSSSSIPSAEKVPTT
S202-p EKVPTTKstLWQEEM
T203-p KVPTTKstLWQEEMR
T218-p AKDQPDGtsLsPAQs
S219-p KDQPDGtsLsPAQsP
S221-p QPDGtsLsPAQsPsQ
S225-p tsLsPAQsPsQsQPP
S227-p LsPAQsPsQsQPPAA
S229-p PAQsPsQsQPPAACT
S252-p KEDESTIsGDRVDGG
S266-p GRKVRVEsGyFsLEK
Y268-p KVRVEsGyFsLEKAK
S270-p RVEsGyFsLEKAKQD
S289-p EQLPPLLsPPsPstP
S292-p PPLLsPPsPstPHsR
S294-p LLsPPsPstPHsRRs
T295-p LsPPsPstPHsRRsQ
S298-p PsPstPHsRRsQVIE
S301-p stPHsRRsQVIEKFE
T320-p EKAEHMEtNMLILTT
T327 tNMLILTTPSSDTRQ
K345 ERRAIPRKRDFASEA
A349 IPRKRDFASEAPtAP
T354-p DFASEAPtAPLSDAC
C361 tAPLSDACPLsPHRR
- gap
S364-p LSDACPLsPHRRAKS
S371 sPHRRAKSLDRRsTE
S376-p AKSLDRRsTEssMTP
T377 KSLDRRsTEssMTPD
S379-p LDRRsTEssMTPDLL
S380-p DRRsTEssMTPDLLN
K389 TPDLLNFKKGWLTkQ
K390 PDLLNFKKGWLTkQY
K395-u FKKGWLTkQYEDGQW
Y449 EYPVQRNYGFQIHTK
S485-p MKHVLPAsAPDVTSS
S502-p EGKNKSTsFETCSRS
K525 GEPDPEQKKSRARER
S538-p ERRREGRsKtFDWAE
T540-p RREGRsKtFDWAEFR
T599-p KRFGMLDtIDGPGME
I600 RFGMLDtIDGPGMED
S617-p LRMDIDRsPGLLGtP
T623-p RsPGLLGtPDLKTQN
P624 sPGLLGtPDLKTQNV
S661-p PIAPLHLsLEDRSER
S670-p EDRSERLstHELTSL
T671-p DRSERLstHELTSLL
S690 EQSQKEASDLLEQNR
S712 VALGREQSAREGYVL
Y717 EQSAREGYVLQATCE
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S814 QYLEELQSVQRELEV
L890 GGGESTGLPLTQGKD
Y914 VKESEIQYLKQEISS
S920 QYLKQEISSLKDELQ
Y944-p SDKYKDIyTELSIAK
T945 DKYKDIyTELSIAKA
T969-p KEQLKAAtEALGEKs
K975 AtEALGEKsPEGTTV
S976-p tEALGEKsPEGTTVS
S983 sPEGTTVSGYDIMKs
Y985 EGTTVSGYDIMKsKs
S990-p SGYDIMKsKsNPDFL
S992-p YDIMKsKsNPDFLKK
S1013-p RQLRNIRsKsVIEQV
S1015-p LRNIRsKsVIEQVsW
S1021-p KsVIEQVsWDN____
  RIP3 iso6  
Y148 WLEMLMVYPRTNKQN
S180 AKMAVTSSSGGSSGS
S188 SGGSSGSSSSIPSAE
S190 GSSGSSSSIPSAEKV
- gap
- gap
S193 GSSSSIPSAEKVPTT
S202 EKVPTTKSTLWQEEM
T203 KVPTTKSTLWQEEMR
T218 AKDQPDGTsLsPAQs
S219-p KDQPDGTsLsPAQsP
S221-p QPDGTsLsPAQsPsQ
S225-p TsLsPAQsPsQsQPP
S227-p LsPAQsPsQsQPPAA
S229-p PAQsPsQsQPPAACT
S252 KEDESTISGDRVDGG
S266 GRKVRVESGYFSLEK
Y268 KVRVESGYFSLEKAK
S270 RVESGYFSLEKAKQD
S289-p EQLPPLLsPPsPstP
S292-p PPLLsPPsPstPHsR
S294-p LLsPPsPstPHsRRS
T295-p LsPPsPstPHsRRSQ
S298-p PsPstPHsRRSQVIE
S301 stPHsRRSQVIEKFE
T320 EKAEHMETNMLILTT
T327 TNMLILTTPSSDTRQ
K345 ERRAIPRKRPDLLNF
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
K353 RPDLLNFKKGWLTKQ
K354 PDLLNFKKGWLTKQY
K359 FKKGWLTKQYEDGQW
Y413 EYPVQRNYGFQIHTK
S449 MKHVLPASAPDVTSS
S466 EGKNKSTSFETCSRS
K489 GEPDPEQKKSRARER
S502-p ERRREGRsKtFDWAE
T504-p RREGRsKtFDWAEFR
T563 KRFGMLDTIDGPGME
I564 RFGMLDTIDGPGMED
S581-p LRMDIDRsPGLLGtP
T587-p RsPGLLGtPDLKTQN
P588 sPGLLGtPDLKTQNV
S625 PIAPLHLSLEDRSER
S634 EDRSERLSTHELTSL
T635 DRSERLSTHELTSLL
S654 EQSQKEASDLLEQNR
S676 VALGREQSAREGYVL
Y681 EQSAREGYVLQTEVA
S998-p EHSHHRVsVQLQSVR
S1046-p KMIALGSsLEETEIK
S1068-p LRRFVSDsPKDAKEP
S1364-p KALQPWAsPAESRTQ
Y1676-p CLEGLGQyssLLVQD
S1677-p LEGLGQyssLLVQDA
S1678-p EGLGQyssLLVQDAI
S2046 QYLEELQSVQRELEV
L2122 GGGESTGLPLTQGKD
Y2146 VKESEIQYLKQEISS
S2152 QYLKQEISSLKDELQ
Y2176 SDKYKDIYTELSIAK
T2177 DKYKDIYTELSIAKA
T2201 KEQLKAATEALGEKs
K2207 ATEALGEKsPEGTTV
S2208-p TEALGEKsPEGTTVS
S2215 sPEGTTVSGYDIMKS
Y2217 EGTTVSGYDIMKSKs
S2222 SGYDIMKSKsNPDFL
S2224-p YDIMKSKsNPDFLKK
S2245 RQLRNIRSKsLKEGL
S2247-p LRNIRSKsLKEGLTV
- gap
  rat

 
Y148-p WLEMLMVyPRTNKQN
S180-p AKMAVTSsSGGSSGS
S188-p SGGSSGSsSsssSSS
S190-p GSSGSsSsssSSSIP
S191-p SSGSsSsssSSSIPS
S192-p SGSsSsssSSSIPSA
S198 ssSSSIPSAEKVPTT
S207 EKVPTTKSTLWQEEM
T208 KVPTTKSTLWQEEMR
T223-p AKDQPDGtSLsPVQS
S224 KDQPDGtSLsPVQSP
S226-p QPDGtSLsPVQSPsQ
S230 tSLsPVQSPsQSQPP
S232-p LsPVQSPsQSQPPAA
S234 PVQSPsQSQPPAACT
S257 KEDENILSGDRVDGG
S271 GRKVRVESGYFSLEK
Y273 KVRVESGYFSLEKAK
S275 RVESGYFSLEKAKQD
S294 EQLPPLLSPPSPSTP
S297 PPLLSPPSPSTPHSR
S299 LLSPPSPSTPHSRRS
T300 LSPPSPSTPHSRRSQ
S303 PSPSTPHSRRSQVIE
S306 STPHSRRSQVIEKFE
T325 EKAEHMETNMLILTT
T332 TNMLILTTPSSDTRQ
K350 ERRAIPRKRDFASET
A354 IPRKRDFASETPTAP
T359 DFASETPTAPLSDAC
C366 TAPLSDACPLSPHRR
- gap
S369 LSDACPLSPHRRAKS
S376 SPHRRAKSLDRRSTE
S381 AKSLDRRSTESSMTP
T382 KSLDRRSTESSMTPD
S384 LDRRSTESSMTPDLL
S385 DRRSTESSMTPDLLN
K394 TPDLLNFKKGWLTKQ
K395 PDLLNFKKGWLTKQY
K400 FKKGWLTKQYEDGQW
Y454 EYPVQRNYGFQIHTK
S490 MKHVLPTSAPDVTSS
S507 EGKNKSTSFDTCLRP
K530 GEPDPEQKKSRARER
S543 ERRREGRSKTFDWAE
T545 RREGRSKTFDWAEFR
T604 KRFGMLDTNDGPGME
N605 RFGMLDTNDGPGMED
S622 LRMDIDRSPGLLGTP
T628 RSPGLLGTPDLKTQN
P629 SPGLLGTPDLKTQNV
S666 PIAPLHLSLEDRSER
S675-p EDRSERLsTHELTSL
T676 DRSERLsTHELTSLL
S695 EQSQKEASDLLEQNR
S717 VALGREQSAREGYVL
Y722 EQSAREGYVLQATCE
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S819 QYLEELQSVQRELEV
L895 GGGESTGLPLTQGKD
Y919 VKESEIQYLKQEISS
S925 QYLKQEISSLKDELQ
Y949 SDKYKDIYTELSIAK
T950 DKYKDIYTELSIAKA
T974 KEQLKAATEALGEKS
K980 ATEALGEKSPEGTTV
S981 TEALGEKSPEGTTVS
S988 SPEGTTVSGYDIMKs
Y990 EGTTVSGYDIMKsKs
S995-p SGYDIMKsKsNPDFL
S997-p YDIMKsKsNPDFLKK
S1018 RQLRNIRSKSVIEQV
S1020 LRNIRSKSVIEQVSW
S1026 KSVIEQVSWDN____
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