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Protein Page:
GTF2F1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
GTF2F1 TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. Heterodimer of an alpha and a beta subunit. Interacts with GTF2F2, CTDP1, TAF6/TAFII80 and URI1. Up-regulated in response to enterovirus 71 (EV71) infection. Belongs to the TFIIF alpha subunit family. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Transcription initiation complex; Kinase, protein; ATYPICAL group; GTF2F1 family
Cellular Component: nucleoplasm; transcription factor TFIIF complex; nucleus; cell junction
Molecular Function: protein binding; DNA binding; phosphatase activator activity; transcription coactivator activity; transcription factor binding; catalytic activity
Biological Process: nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; positive regulation of catalytic activity; transcription initiation from RNA polymerase II promoter; mRNA capping; viral reproduction; positive regulation of viral transcription; response to virus; RNA splicing; RNA elongation from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; gene expression
Reference #:  P35269 (UniProtKB)
Alt. Names/Synonyms: BTF4; General transcription factor IIF 74 kDa subunit; General transcription factor IIF subunit 1; general transcription factor IIF, polypeptide 1, 74kDa; GTF2F1; RAP74; T2FA; TF2F1; TFIIF; TFIIF-alpha; Transcription initiation factor IIF subunit alpha; Transcription initiation factor RAP74
Gene Symbols: GTF2F1
Molecular weight: 58,240 Da
Basal Isoelectric point: 7.04  Predict pI for various phosphorylation states
CST Pathways:  Protein Acetylation
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

GTF2F1

Protein Structure Not Found.


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Sites Implicated In
transcription, inhibited: S385‑p, T389‑p
activity, inhibited: S385‑p, T389‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 K54 LERDLSNKkIyQEEE
0 1 K55-ub ERDLSNKkIyQEEEM
0 1 Y57-p DLSNKkIyQEEEMPE
0 1 K74 AGSEFNRKLREEARR
0 65 T154-p TPLARHRtLtAEEAE
0 208 T156-p LARHRtLtAEEAEEE
0 1 K169-ub EEWERRNkVLNHFSI
0 1 K183-ub IMQQRRLkDQDQDED
0 19 S217-p LEDDLEMssDAsDAs
0 19 S218-p EDDLEMssDAsDAsG
0 20 S221-p LEMssDAsDAsGEEG
0 18 S224-p ssDAsDAsGEEGGRV
0 1 S253-p KKKKKKGsDDEAFED
0 1 S261-p DDEAFEDsDDGDFEG
0 1 D272-ca DFEGQEVdYMSDGSS
0 12 S305-p PKGVDEQsDssEEsE
0 13 S307-p GVDEQsDssEEsEEE
0 13 S308-p VDEQsDssEEsEEEk
0 12 S311-p QsDssEEsEEEkPPE
0 1 K315-ac sEEsEEEkPPEEDKE
0 1 K328-ac KEEEEEKkAPtPQEK
0 9 T331-p EEEKkAPtPQEKKRR
0 1 S341-p EKKRRKDssEEsDss
0 1 S342-p KKRRKDssEEsDssE
0 1 S345-p RKDssEEsDssEEsD
0 1 S347-p DssEEsDssEEsDID
0 2 S348-p ssEEsDssEEsDIDS
0 2 S351-p EsDssEEsDIDSEAS
0 1 S355 sEEsDIDSEASSALF
0 1 S358 sDIDSEASSALFMAK
0 13 T368-p LFMAKKKtPPKRERK
0 89 S377-p PKRERKPsGGssRGN
0 74 S380-p ERKPsGGssRGNsRP
0 62 S381-p RKPsGGssRGNsRPG
0 9 N384 sGGssRGNsRPGtPs
1 253 S385-p GGssRGNsRPGtPsA
1 291 T389-p RGNsRPGtPsAEGGs
0 67 S391-p NsRPGtPsAEGGsTs
0 4 S396-p tPsAEGGsTssTLRA
0 1 S398-p sAEGGsTssTLRAAA
0 1 S399-p AEGGsTssTLRAAAS
0 7 K407-ac TLRAAASkLEQGkRV
0 1 K412-ac ASkLEQGkRVsEMPA
0 1 R413 SkLEQGkRVsEMPAA
0 1 S415-p LEQGkRVsEMPAAkR
0 26 K421-ac VsEMPAAkRLRLDtG
0 124 T427-p AkRLRLDtGPQsLsG
0 23 S431-p RLDtGPQsLsGKstP
0 47 S433-p DtGPQsLsGKstPQP
0 23 S436-p PQsLsGKstPQPPsG
0 22 T437-p QsLsGKstPQPPsGK
0 8 S442-p KstPQPPsGKttPNs
1 4 T445-p PQPPsGKttPNsGDV
1 10 T446-p QPPsGKttPNsGDVQ
0 1 N448 PsGKttPNsGDVQVt
0 3 S449-p sGKttPNsGDVQVtE
0 1 T455-p NsGDVQVtEDAVRRY
0 3 K466-ac VRRYLTRkPMTTkDL
0 3 K471-ac TRkPMTTkDLLKKFQ
0 1 K510-ub ERKMINDkMHFSLKE
0 2 S514 INDkMHFSLKE____
  mouse

 
K54-ac LERDLSNkKIYQEEE
K55 ERDLSNkKIYQEEEM
Y57 DLSNkKIYQEEEMPE
K74-ac AGSEFNRkLREEARR
T154-p TPLARHRtLtAEEAE
T156-p LARHRtLtAEEAEEE
K169 EEWERRNKVLNHFSI
K183 IMQQRRLKDQDQDED
S217-p LEDDLEMssDAsDAs
S218-p EDDLEMssDAsDAsG
S221-p LEMssDAsDAsGEEG
S224-p ssDAsDAsGEEGSRT
S253 KKKKKKGSDDEAFED
S261 DDEAFEDSDDGDFEG
D272 DFEGQEVDYMSDGSS
S305-p PKGVDEQsEssEEsE
S307-p GVDEQsEssEEsEEE
S308-p VDEQsEssEEsEEEK
S311-p QsEssEEsEEEKPPE
K315 sEEsEEEKPPEEDKE
K328 KEEEEEKKAPtPQEK
T331-p EEEKKAPtPQEKKRR
S341 EKKRRKDSSDDSDSS
S342 KKRRKDSSDDSDSSE
S345 RKDSSDDSDSSEESD
S347 DSSDDSDSSEESDID
S348 SSDDSDSSEESDIDS
S351 DSDSSEESDIDSETS
S355 SEESDIDSETSSALF
S358 SDIDSETSSALFMAK
T368-p LFMAKKKtPPKRERK
S377-p PKRERKPsGGssKGt
S380-p ERKPsGGssKGtsRP
S381-p RKPsGGssKGtsRPG
T384-p sGGssKGtsRPGtPs
S385-p GGssKGtsRPGtPsA
T389-p KGtsRPGtPsAEAAS
S391-p tsRPGtPsAEAASTS
S396 tPsAEAASTSSTLRA
S398 sAEAASTSSTLRAAA
S399 AEAASTSSTLRAAAS
K407 TLRAAASKLEQGKrT
K412 ASKLEQGKrTSETPA
R413-m1 SKLEQGKrTSETPAA
S415 LEQGKrTSETPAAKR
K421 TSETPAAKRLRMDtG
T427-p AKRLRMDtGPQsLsG
S431-p RMDtGPQsLsGKstP
S433-p DtGPQsLsGKstPss
S436-p PQsLsGKstPssGDV
T437-p QsLsGKstPssGDVQ
- gap
- gap
- gap
S439-p LsGKstPssGDVQVt
S440-p sGKstPssGDVQVtE
T446-p ssGDVQVtEDAVRRY
K457 VRRYLTRKPMTTKDL
K462 TRKPMTTKDLLKKFQ
K501 ERKMIGDKMHFsLKE
S505-p IGDKMHFsLKE____
  rat

 
K54 LERDLSNKKIYQEEE
K55 ERDLSNKKIYQEEEM
Y57 DLSNKKIYQEEEMPE
K74 AGSEFNRKLREEARR
T154 TPLARHRTLTAEEAE
T156 LARHRTLTAEEAEEE
K169 EEWERRNKVLNHFSI
K183 IMQQRRLKDQDQDED
S217 LEDDLEMSSDASDAS
S218 EDDLEMSSDASDASG
S221 LEMSSDASDASGEEG
S224 SSDASDASGEEGSRA
S253 KKKKKKGSDDEAFED
S261 DDEAFEDSDDGDFEG
D272 DFEGQEVDYMSDGSS
S305 PKGVDEQSESSEESE
S307 GVDEQSESSEESEEE
S308 VDEQSESSEESEEEK
S311 QSESSEESEEEKPPE
K315 SEESEEEKPPEEDKE
K328 KEEEEEKKAPtPQEK
T331-p EEEKKAPtPQEKKRR
S341 EKKRRKDSSDDSDSs
S342 KKRRKDSSDDSDSsE
S345 RKDSSDDSDSsEEsD
S347 DSSDDSDSsEEsDID
S348-p SSDDSDSsEEsDIDs
S351-p DSDSsEEsDIDsETs
S355-p sEEsDIDsETsSALF
S358-p sDIDsETsSALFMAK
T368 LFMAKKKTPPKRERK
S377 PKRERKPSGGSSKGT
S380 ERKPSGGSSKGTSRP
S381 RKPSGGSSKGTSRPG
T384 SGGSSKGTSRPGtPS
S385 GGSSKGTSRPGtPSA
T389-p KGTSRPGtPSAEAAS
S391 TSRPGtPSAEAASTS
S396 tPSAEAASTSSTLRA
S398 SAEAASTSSTLRAAA
S399 AEAASTSSTLRAAAS
K407 TLRAAASKLEQGKRT
K412 ASKLEQGKRTSETPA
R413 SKLEQGKRTSETPAA
S415 LEQGKRTSETPAAKR
K421 TSETPAAKRLRMDTG
T427 AKRLRMDTGPQSLSG
S431 RMDTGPQSLSGKSTP
S433 DTGPQSLSGKSTPSS
S436 PQSLSGKSTPSSGDV
T437 QSLSGKSTPSSGDVQ
- gap
- gap
- gap
S439 LSGKSTPSSGDVQVT
S440 SGKSTPSSGDVQVTE
T446 SSGDVQVTEDAVRRY
K457 VRRYLTRKPMTTKDL
K462 TRKPMTTKDLLKKFQ
K501 ERKMIGDKMHFSLKE
S505 IGDKMHFSLKE____
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