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Protein Page:
THEX1 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
THEX1 RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication- dependent histone pre-mRNAs. Identified in a histone pre-mRNA complex, at least composed of ERI1, LSM11, SLBP, SNRPB, SYNCRIP and YBX1. Interacts in a cooperative manner with SLBP to the mature 3'-end of histone mRNAs. Binds to 40S and 60S ribosomal subunits and to 80S assembled ribosomes. Found in a ternary complex with SLBP and the stem-loop structure of the 3'-end of histone mRNAs. Although it can bind simultaneously with SLBP to the 3'-end of histone mRNA, the presence of SLBP prevents the exonuclease activity. Note: This description may include information from UniProtKB.
Protein type: EC 3.1.-.-; Hydrolase
Cellular Component: cytoplasm; nucleolus; nucleus
Molecular Function: rRNA binding; metal ion binding; ribosome binding; 3'-5' exonuclease activity
Biological Process: RNA-mediated gene silencing; rRNA 3'-end processing
Reference #:  Q8IV48 (UniProtKB)
Alt. Names/Synonyms: 3' exoribonuclease; 3'-5' exonuclease ERI1; 3'-5' exoribonuclease 1; 3'EXO; 3'HEXO; enhanced RNAi three prime mRNA exonuclease homolog 1; Eri-1 homolog; ERI1; exoribonuclease 1; HEXO; histone mRNA 3' end-specific exonuclease; Histone mRNA 3'-end-specific exoribonuclease; Histone mRNA 3'-exonuclease 1; MGC35395; Protein 3'hExo; THEX1; three prime histone mRNA exonuclease 1; three prime mRNA exonuclease 1
Gene Symbols: ERI1
Molecular weight: 40,064 Da
Basal Isoelectric point: 6.29  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

THEX1

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
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Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 K52-u GQETKGSkFITSsAs
0 1 S57-p GSkFITSsAsDFsDP
0 6 S59-p kFITSsAsDFsDPVy
0 4 S62-p TSsAsDFsDPVyKEI
0 174 Y66-p sDFsDPVyKEIAITN
0 2 S120 QKLMLKESNFADSYY
0 2 N121 KLMLKESNFADSYYD
0 1 K249 QCQLSRLKYPPFAKK
0 1 K269-u KSYGNFYkVPRSQTK
  mouse

 
K48 GKETDGSKFISSNGS
N53 GSKFISSNGSDFSDP
S55 KFISSNGSDFSDPVy
S58 SSNGSDFSDPVyKEI
Y62-p SDFSDPVyKEIAMTN
S116-p QKLMLKEssAGDSYY
S117-p KLMLKEssAGDSYYD
K245-u QCRLSRLkHPAFAKK
K265 KSYGNFYKVPRSQTK
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