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Protein Page:
Notch 1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
Notch 1 Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Heterodimer of a C-terminal fragment N(TM) and an N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with DNER, DTX1, DTX2 and RBPJ/RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1. The activated membrane-bound form interacts with AAK1 which promotes NOTCH1 stabilization. Forms a trimeric complex with FBXW7 and SGK1. Interacts with HIF1AN. HIF1AN negatively regulates the function of notch intracellular domain (NICD), accelerating myogenic differentiation. In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues. Belongs to the NOTCH family. Note: This description may include information from UniProtKB.
Protein type: Receptor, misc.; Membrane protein, integral; Transcription factor; Motility/polarity/chemotaxis
Cellular Component: Golgi membrane; nucleoplasm; endoplasmic reticulum membrane; cell surface; extracellular region; plasma membrane; integral to membrane; cytosol; nucleus; receptor complex
Molecular Function: enzyme inhibitor activity; protein binding; enzyme binding; sequence-specific DNA binding; chromatin DNA binding; receptor activity; calcium ion binding; transcription factor activity
Biological Process: neural tube development; negative regulation of calcium ion-dependent exocytosis; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; heart development; positive regulation of JAK-STAT cascade; negative regulation of BMP signaling pathway; compartment specification; negative regulation of ossification; somatic stem cell division; negative regulation of osteoblast differentiation; positive regulation of keratinocyte differentiation; positive regulation of cardiac muscle cell proliferation; positive regulation of astrocyte differentiation; negative regulation of photoreceptor cell differentiation; Notch receptor processing; keratinocyte differentiation; branching morphogenesis of a tube; response to muramyl dipeptide; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; determination of left/right symmetry; anagen; positive regulation of epithelial cell proliferation; foregut morphogenesis; endoderm development; cell fate specification; cardiac muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; negative regulation of neurogenesis; negative regulation of cell proliferation; glial cell differentiation; regulation of transcription, DNA-dependent; forebrain development; positive regulation of cell proliferation; cardiac muscle morphogensis; heart looping; regulation of somitogenesis; positive regulation of BMP signaling pathway; mesenchymal cell development; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; hair follicle morphogenesis; in utero embryonic development; lumen formation; liver development; humoral immune response; activation of Notch receptor target transcription factor; negative regulation of oligodendrocyte differentiation; inflammatory response to antigenic stimulus; axonogenesis; negative regulation of catalytic activity; epithelial to mesenchymal transition; gene expression; immune response; sprouting angiogenesis; negative regulation of myoblast differentiation; auditory receptor cell fate commitment; lung development; positive regulation of cell migration
Reference #:  P46531 (UniProtKB)
Alt. Names/Synonyms: hN1; Neurogenic locus notch homolog protein 1; NOTC1; Notch 1; Notch 1 extracellular truncation; Notch 1 intracellular domain; Notch homolog 1, translocation-associated (Drosophila); NOTCH1; TAN1; Translocation-associated notch protein TAN-1
Gene Symbols: NOTCH1
Molecular weight: 272,505 Da
Basal Isoelectric point: 4.95  Predict pI for various phosphorylation states
CST Pathways:  ESC Pluripotency and Differentiation  |  Notch Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Notch 1

Protein Structure Not Found.


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Sites Implicated In
transcription, induced: T1897‑p, S1900‑p
protein degradation: S2521‑p, S2522‑p, S2523‑p, S2524‑p
receptor inactivation, altered: S2521‑p, S2522‑p, S2523‑p, S2524‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S900-p CHCQAGYsGRNCETD
0 1 K1317-ac GCKGKPCkNGGTCAV
1 1 K1774 LWFPEGFKVSEASKK
1 1 K1780 FKVSEASKKKRREPL
1 1 K1781 KVSEASKKKRREPLG
1 1 K1782 VSEASKKKRREPLGE
1 1 K1795 GEDSVGLKPLKNASD
0 1 K1795 GEDSVGLKPLKNASD
0 1 K1821-ub GDEDLETkKFRFEEP
0 1 T1861 RMSAMAPTPPQGEVD
2 0 T1897-p CSGGGLEtGNsEEEE
2 0 S1900-p GGLEtGNsEEEEDAP
0 1 T1927-p LHNQTDRtGEtALHL
0 1 T1930-p QTDRtGEtALHLAAR
1 1 K1945 YSRSDAAKRLLEAsA
0 2 S1951-p AKRLLEAsADANIQD
1 1 R2060 NKDMQNNREETPLFL
0 1 Y2074-p LAAREGSyETAKVLL
1 1 K2078 EGSyETAKVLLDHFA
0 1 S2136-p LGGTPTLsPPLCSPN
0 5 Y2145-p PLCSPNGyLGSLKPG
1 1 K2156 LKPGVQGKKVRKPsS
1 1 K2157 KPGVQGKKVRKPsSK
1 1 K2160 VQGKKVRKPsSKGLA
0 3 S2162-p GKKVRKPsSKGLACG
1 1 K2164 KVRKPsSKGLACGSK
1 1 K2171 KGLACGSKEAKDLKA
1 1 K2174 ACGSKEAKDLKARRK
0 5 Y2323-p SGMVPNQyNPLRGSV
1 0 T2511 VPEHPFLTPSPESPD
1 0 S2521-p PESPDQWssssPHSN
1 0 S2522-p ESPDQWssssPHSNV
1 0 S2523-p SPDQWssssPHSNVS
1 0 S2524-p PDQWssssPHSNVSD
  mouse

 
T900 CLCQAGYTGRNCESD
K1317 GCRGKPCKNGGVCAV
K1764-ac LWFPEGFkVSEASkk
K1770-ac FkVSEASkkkRREPL
K1771-ac kVSEASkkkRREPLG
K1772-ac VSEASkkkRREPLGE
K1785-ac GEDSVGLkPLKNASD
K1785-ub GEDSVGLkPLKNASD
K1811 GDEDLETKKFRFEEP
T1851-p RMSAMAPtPPQGEVD
T1887 CSGGGLETGNSEEEE
S1890 GGLETGNSEEEEDAP
T1917 LHNQTDRTGETALHL
T1920 QTDRTGETALHLAAR
K1935-ac YSRSDAAkRLLEASA
S1941 AkRLLEASADANIQD
K2050-ac NKDMQNNkEETPLFL
Y2064 LAAREGSYETAkVLL
K2068-ac EGSYETAkVLLDHFA
S2126 LGGTPTLSPTLCSPN
Y2135 TLCSPNGYLGNLKSA
K2146-ac LKSATQGkkARkPsT
K2147-ac KSATQGkkARkPsTk
K2150-ac TQGkkARkPsTkGLA
S2152-p GkkARkPsTkGLACG
K2154-ac kARkPsTkGLACGSk
K2161-ac kGLACGSkEAkDLKA
K2164-ac ACGSkEAkDLKARRK
Y2313 NGMVPSQYNPLRPGV
T2487-p VPEHPFLtPSPESPD
S2497 PESPDQWSSSSPHSN
S2498 ESPDQWSSSSPHSNI
S2499 SPDQWSSSSPHSNIS
S2500 PDQWSSSSPHSNISD
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