Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Protein Page:
TNF-a (mouse)

Overview
TNF-a Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Homotrimer. Interacts with SPPL2B. Belongs to the tumor necrosis factor family. Note: This description may include information from UniProtKB.
Protein type: Apoptosis; Membrane protein, integral; Motility/polarity/chemotaxis; Cytokine
Cellular Component: extracellular space; recycling endosome; cell surface; membrane; integral to plasma membrane; plasma membrane; integral to membrane; extracellular region; intracellular; phagocytic cup; external side of plasma membrane; secretory granule; lipid raft
Molecular Function: identical protein binding; protein binding; protease binding; cytokine activity; tumor necrosis factor receptor binding
Biological Process: positive regulation of JNK activity; extracellular matrix organization and biogenesis; positive regulation of nitric oxide biosynthetic process; positive regulation of NFAT protein import into nucleus; positive regulation of apoptosis; positive regulation of osteoclast differentiation; activation of MAPK activity; multicellular organismal development; positive regulation of transcription, DNA-dependent; response to glucocorticoid stimulus; positive regulation of caspase activity; positive regulation of NF-kappaB import into nucleus; osteoclast differentiation; positive regulation of translational initiation by iron; positive regulation of membrane protein ectodomain proteolysis; activation of NF-kappaB transcription factor; positive regulation of MAP kinase activity; cellular extravasation; tumor necrosis factor-mediated signaling pathway; negative regulation of interleukin-6 production; JNK cascade; negative regulation of osteoblast differentiation; positive regulation of action potential; regulation of immunoglobulin secretion; negative regulation of protein complex disassembly; positive regulation of cytokine production; positive regulation of heterotypic cell-cell adhesion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of mitosis; response to virus; positive regulation of interleukin-8 biosynthetic process; glucose metabolic process; positive regulation of interleukin-6 production; positive regulation of chemokine production; negative regulation of cytokine secretion during immune response; positive regulation of protein transport; cell activation; detection of mechanical stimulus involved in sensory perception of pain; defense response to Gram-positive bacterium; organ morphogenesis; DNA damage response, signal transduction resulting in induction of apoptosis; induction of apoptosis via death domain receptors; defense response to bacterium; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of L-glutamate transport; negative regulation of transcription, DNA-dependent; leukocyte migration; sequestering of triacylglycerol; apoptosis; positive regulation of smooth muscle cell proliferation; positive regulation of JNK cascade; defense response; negative regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-18 production; signal transduction; chronic inflammatory response to antigenic stimulus; positive regulation of synaptic transmission; positive regulation of hair follicle development; negative regulation of cell proliferation; regulation of osteoclast differentiation; regulation of protein secretion; negative regulation of lipid catabolic process; positive regulation of neuron apoptosis; positive regulation of cell proliferation; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; positive regulation of chronic inflammatory response to antigenic stimulus; regulation of I-kappaB kinase/NF-kappaB cascade; inflammatory response; caspase activation; positive regulation of humoral immune response mediated by circulating immunoglobulin; regulation of protein amino acid phosphorylation; positive regulation of protein complex disassembly; transformed cell apoptosis; MAPKKK cascade; calcium-mediated signaling; positive regulation of peptidyl-serine phosphorylation; humoral immune response; regulation of cell proliferation; cell proliferation; positive regulation of protein kinase B signaling cascade; positive regulation of interferon-gamma production; negative regulation of glucose import; positive regulation of programmed cell death; positive regulation of chemokine biosynthetic process; positive regulation of protein complex assembly; negative regulation of viral genome replication; protein import into nucleus, translocation; positive regulation of fever; activation of MAPKKK activity; immune response; positive regulation of protein amino acid phosphorylation; receptor biosynthetic process; leukocyte tethering or rolling; regulation of insulin secretion; positive regulation of cytokine secretion; positive regulation of inflammatory response
Reference #:  P06804 (UniProtKB)
Alt. Names/Synonyms: Cachectin; DIF; MGC151434; Tnf; TNF alpha; TNF-a; TNF-alpha; Tnfa; TNFalpha; Tnfsf1a; Tnfsf2; Tumor necrosis factor; tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha
Gene Symbols: Tnf
Molecular weight: 25,896 Da
Basal Isoelectric point: 5.01  Predict pI for various phosphorylation states
CST Pathways:  Death Receptor Signaling  |  Inhibition of Apoptosis  |  Insulin Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

TNF-a

Protein Structure Not Found.


STRING  |  Reactome  |  BioGPS  |  Scansite  |  Pfam  |  RCSB PDB  |  Phospho3D  |  Phospho.ELM  |  Source  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene


Modification Sites and Domains  

Modification Sites in Parent Protein, Orthologs, and Isoforms  
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       mouse

 
0 1 S2-p ______MstEsMIRD
0 2 T3-p _____MstEsMIRDV
0 2 S5-p ___MstEsMIRDVEL
0 1 S164 TVSRFAISYQEKVNL
0 1 S173 QEKVNLLSAVKSPCP
  human

 
S2 ______MSTESMIRD
T3 _____MSTESMIRDV
S5 ___MSTESMIRDVEL
S162-p TISRIAVsYQTKVNL
S171-p QTKVNLLsAIKSPCQ
  rat

 
S2 ______MSTESMIRD
T3 _____MSTESMIRDV
S5 ___MSTESMIRDVEL
S164 TVSRFAISYQEKVSL
S173 QEKVSLLSAIKSPCP
Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.