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Protein Page:
ZAP70 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
ZAP70 a tyrosine kinase of the Syk family. Associates with the T-cell antigen receptor zeta-chain after TCR stimulation. Phosphorylated by Src-family kinases following antigen receptor activation. Plays a role in lymphocyte activation. Note: This description may include information from UniProtKB.
Protein type: Kinase, protein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; TK group; Syk family
Chromosomal Location of Human Ortholog: 2q12
Cellular Component: T cell receptor complex; cytoplasm; plasma membrane; immunological synapse; cytosol; lipid raft
Molecular Function: protein binding; phosphotyrosine binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding
Biological Process: adaptive immune response; peptidyl-tyrosine phosphorylation; B cell activation; T cell activation; protein amino acid autophosphorylation; positive regulation of calcium-mediated signaling; negative thymic T cell selection; T cell receptor signaling pathway; protein amino acid phosphorylation; positive regulation of T cell differentiation; immune response; positive regulation of alpha-beta T cell proliferation; beta selection; positive regulation of alpha-beta T cell differentiation; T cell differentiation; positive thymic T cell selection
Reference #:  P43403 (UniProtKB)
Alt. Names/Synonyms: 70 kDa zeta-associated protein; FLJ17670; FLJ17679; SRK; STD; Syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; TZK; ZAP-70; ZAP70; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD
Gene Symbols: ZAP70
Molecular weight: 69,872 Da
Basal Isoelectric point: 7.78  Predict pI for various phosphorylation states
CST Pathways:  T Cell Receptor Signaling  |  Tyrosine Kinases & Substrates
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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ZAP70

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
cell adhesion, altered: Y292‑p, Y315‑p, Y493‑p
cell differentiation, altered: Y315‑p
cell growth, altered: Y292‑p
transcription, altered: Y292‑p, Y319‑p, Y492‑p, Y493‑p, S520‑p
transcription, induced: Y319‑p
activity, induced: Y319‑p, Y474‑p
enzymatic activity, induced: Y315‑p, Y493‑p
enzymatic activity, inhibited: Y292‑p, Y492‑p
intracellular localization: Y292‑p, S520‑p
molecular association, regulation: Y292‑p, Y315‑p, Y319‑p, Y474‑p
phosphorylation: Y292‑p, Y315‑p, Y319‑p, Y493‑p, S520‑p
protein degradation: Y292‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 4 Y12-p AAHLPFFyGSISRAE
0 1 K25-ub AEAEEHLkLAGMADG
0 2 Y46-p CLRSLGGyVLSLVHD
1 98 Y69-p ERQLNGTyAIAGGKA
0 1 Y87-p PAELCEFySRDPDGL
0 2 K100-ub GLPCNLRkPCNRPSG
1 50 Y126-p RDAMVRDyVRQTWkL
0 1 K132-ub DyVRQTWkLEGEALE
0 229 Y164-p AHERMPWyHssLTRE
0 3 S166-p ERMPWyHssLTREEA
0 2 S167-p RMPWyHssLTREEAE
0 4 K176-ub TREEAERkLysGAQT
1 168 Y178-p EEAERkLysGAQTDG
0 4 S179-p EAERkLysGAQTDGK
0 3 K193-ub KFLLRPRkEQGtyAL
0 1 T197-p RPRkEQGtyALSLIy
0 4 Y198-p PRkEQGtyALSLIyG
0 7 Y204-p tyALSLIyGkTVyHy
0 1 K206-ub ALSLIyGkTVyHyLI
0 5 Y209-p LIyGkTVyHyLISQD
0 1 Y211-p yGkTVyHyLISQDkA
0 4 K217-ub HyLISQDkAGKyCIP
0 5 Y221-p SQDkAGKyCIPEGTK
0 343 Y248-p LKADGLIyCLKEACP
0 4 S257-p LKEACPNssASNAsG
0 3 S258-p KEACPNssASNAsGA
0 1 S263-p NssASNAsGAAAPTL
0 1 T278-p PAHPSTLtHPQRRID
0 113 T286-p HPQRRIDtLNsDGyt
0 151 S289-p RRIDtLNsDGytPEP
8 447 Y292-p DtLNsDGytPEPARI
0 9 T293-p tLNsDGytPEPARIt
0 21 T300-p tPEPARItsPDKPRP
0 16 S301-p PEPARItsPDKPRPM
0 34 T312-p PRPMPMDtsVyEsPy
0 51 S313-p RPMPMDtsVyEsPys
7 258 Y315-p MPMDtsVyEsPysDP
0 64 S317-p MDtsVyEsPysDPEE
29 259 Y319-p tsVyEsPysDPEELK
0 81 S320-p sVyEsPysDPEELKD
0 1 S351-p LGCGNFGsVRQGVYR
0 2 K361-ub QGVYRMRkkQIDVAI
0 1 K362-ub GVYRMRkkQIDVAIK
0 4 K377-ub VLKQGTEkADTEEMM
0 62 Y397-p MHQLDNPyIVRLIGV
0 1 Y451-p QVSMGMKyLEEkNFV
0 1 K455-ub GMKyLEEkNFVHRDL
1 3 Y474-p VLLVNRHyAkISDFG
0 3 K476-ub LVNRHyAkISDFGLS
0 4 K484-ub ISDFGLSkALGADDs
0 75 S491-p kALGADDsyytARSA
6 421 Y492-p ALGADDsyytARSAG
15 425 Y493-p LGADDsyytARSAGk
0 82 T494-p GADDsyytARSAGkW
0 1 K500-ub ytARSAGkWPLKWyA
0 20 Y506-p GkWPLKWyAPECINF
1 1 S520-p FRKFSSRsDVWSYGV
0 2 Y525 SRsDVWSYGVTMWEA
0 1 S534-p VTMWEALsyGQkPYK
0 3 Y535-p TMWEALsyGQkPYKK
0 1 K538-ub EALsyGQkPYKKMKG
0 2 K556-ub MAFIEQGkRMECPPE
0 1 Y569-p PECPPELyALMSDCW
1 185 Y597-p EQRMRACyysLASkV
1 161 Y598-p QRMRACyysLASkVE
0 9 S599-p RMRACyysLASkVEG
0 1 K603-ac CyysLASkVEGPPGS
0 4 K603-ub CyysLASkVEGPPGS
0 1 T611-p VEGPPGStQkAEAAC
0 14 K613-ub GPPGStQkAEAACA_
2701 : Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody
2717 : Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb
2704 : Phospho-Zap-70 (Tyr493) Antibody
  mouse

 
Y12 AAHLPFFYGSISRAE
K25 AEAEEHLKLAGMADG
Y46 CLRSLGGYVLSLVHD
Y69 ERQLNGTYAIAGGKA
Y87 PAELCQFYSQDPDGL
K100 GLPCNLRKPCNRPPG
Y126 RDAMVRDYVRQTWKL
K132 DYVRQTWKLEGDALE
Y164-p AHERMPWyHSSLTRE
S166 ERMPWyHSSLTREEA
S167 RMPWyHSSLTREEAE
K176 TREEAERKLYSGQQT
Y178 EEAERKLYSGQQTDG
S179 EAERKLYSGQQTDGK
K193 KFLLRPRKEQGTYAL
T197 RPRKEQGTYALSLVY
Y198 PRKEQGTYALSLVYG
Y204 TYALSLVYGKTVYHY
K206 ALSLVYGKTVYHYLI
Y209 LVYGKTVYHYLISQD
Y211 YGKTVYHYLISQDKA
K217 HYLISQDKAGKYCIP
Y221 SQDKAGKYCIPEGTK
Y248 LKADGLIYRLKEVCP
S257 LKEVCPNSSASAAVA
S258 KEVCPNSSASAAVAA
- gap
T276 PAHPSTFTQPQRRVD
T284 QPQRRVDTLNSDGyT
S287 RRVDTLNSDGyTPEP
Y290-p DTLNSDGyTPEPARL
T291 TLNSDGyTPEPARLA
S299 PEPARLASSTDKPRP
S300 EPARLASSTDKPRPM
T311 PRPMPMDTSVyESPy
S312 RPMPMDTSVyESPyS
Y314-p MPMDTSVyESPySDP
S316 MDTSVyESPySDPEE
Y318-p TSVyESPySDPEELK
S319 SVyESPySDPEELKD
S350 LGCGNFGSVRQGVYR
K360 QGVYRMRKKQIDVAI
K361 GVYRMRKKQIDVAIK
K376 VLKQGTEKADKDEMM
Y396 MHQLDNPYIVRLIGV
Y450 QVAMGMKYLEEKNFV
K454 GMKYLEEKNFVHRDL
Y473 VLLVNRHYAKISDFG
K475 LVNRHYAKISDFGLS
K483 ISDFGLSKALGADDs
S490-p KALGADDsyyTARSA
Y491-p ALGADDsyyTARSAG
Y492-p LGADDsyyTARSAGK
T493 GADDsyyTARSAGKW
K499 yTARSAGKWPLKWYA
Y505 GKWPLKWYAPECINF
S519 FRKFSSRSDVWSyGV
Y524-p SRSDVWSyGVTMWEA
S533 VTMWEAFSyGQKPYK
Y534-p TMWEAFSyGQKPYKK
K537 EAFSyGQKPYKKMKG
K555 LDFIKQGKRMECPPE
Y568 PECPPEMYALMSDCW
Y596 EQRMRNYYYSLASRA
Y597 QRMRNYYYSLASRAE
S598 RMRNYYYSLASRAEG
R602 YYYSLASRAEGPPQC
R602 YYYSLASRAEGPPQC
E610 AEGPPQCEQVAEAAC
V612 GPPQCEQVAEAACG_
2701 : Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody
2717 : Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb
2704 : Phospho-Zap-70 (Tyr493) Antibody
  rat

 
Y12 AAHLPFFYGSISRAE
K25 AEAEEHLKLAGMADG
Y46 CLRSLGGYVLSLVHD
Y69-p ERQLNGTyAIAGGKA
Y87 PAELCQFYSQDPDGL
K100 GLPCNLRKPCNRPPG
Y126 RDAMVRDYVRQTWKL
K132 DYVRQTWKLEGDALE
Y164 AHERMPWYHSSLTRE
S166 ERMPWYHSSLTREEA
S167 RMPWYHSSLTREEAE
K176 TREEAERKLYSGQQT
Y178 EEAERKLYSGQQTDG
S179 EAERKLYSGQQTDGK
K193 KFLLRPRKEQGTYAL
T197 RPRKEQGTYALSLVY
Y198 PRKEQGTYALSLVYG
Y204 TYALSLVYGKTVYHY
K206 ALSLVYGKTVYHYLI
Y209 LVYGKTVYHYLISQD
Y211 YGKTVYHYLISQDKA
K217 HYLISQDKAGKYCIP
Y221 SQDKAGKYCIPEGTK
Y248 LKADGLIYRLKEVCP
S257 LKEVCPNSSASAEAA
S258 KEVCPNSSASAEAAA
- gap
T276 PAHPSTFTQPHRRID
T284 QPHRRIDTLNSDGYT
S287 RRIDTLNSDGYTPEP
Y290 DTLNSDGYTPEPARL
T291 TLNSDGYTPEPARLD
- gap
- gap
T307 PRPMPMDTSVYESPY
S308 RPMPMDTSVYESPYS
Y310 MPMDTSVYESPYSDP
S312 MDTSVYESPYSDPEE
Y314 TSVYESPYSDPEELK
S315 SVYESPYSDPEELKD
S346 LGCGNFGSVRQGVYR
K356 QGVYRMRKKQIDVAI
K357 GVYRMRKKQIDVAIK
K372 VLKQSTEKADKDEMM
Y392 MHQLDNPYIVRLIGV
Y446 QVAMGMKYLEEKNFV
K450 GMKYLEEKNFVHRDL
Y469 VLLVNRHYAKISDFG
K471 LVNRHYAKISDFGLS
K479 ISDFGLSKALGADDS
S486 KALGADDSyYTARSA
Y487-p ALGADDSyYTARSAG
Y488 LGADDSyYTARSAGK
T489 GADDSyYTARSAGKW
K495 YTARSAGKWPLKWYA
Y501 GKWPLKWYAPECINF
S515 FRKFSSRSDVWSYGV
Y520 SRSDVWSYGVTMWEA
S529 VTMWEAFSYGQKPYK
Y530 TMWEAFSYGQKPYKK
K533 EAFSYGQKPYKKMKG
K551 LDFIKQGKRMECPPE
Y564 PECPPEMYALMSDCW
Y592 EQRMRTYYYSMASRA
Y593 QRMRTYYYSMASRAE
S594 RMRTYYYSMASRAEG
R598 YYYSMASRAEGPPQC
R598 YYYSMASRAEGPPQC
E606 AEGPPQCEQVAEAAC
V608 GPPQCEQVAEAACG_
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