Accepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation. Interacts with UBA3, DCUN1D1 and RBX1. Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily. Note: This description may include information from UniProtKB.
Protein type: Ligase; Adaptor/scaffold; EC 22.214.171.124; Ubiquitin ligase; EC 6.3.2.-; Ubiquitin conjugating system
Cellular Component: cytosol
Molecular Function: protein binding; ribosomal S6-glutamic acid ligase activity; ubiquitin-protein ligase activity; ATP binding; NEDD8 ligase activity
Biological Process: protein neddylation; positive regulation of neuron apoptosis; transforming growth factor beta receptor signaling pathway; protein ubiquitination; protein modification process
SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.