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Protein Page:
UBE2M (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
UBE2M Accepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation. Interacts with UBA3, DCUN1D1 and RBX1. Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily. Note: This description may include information from UniProtKB.
Protein type: Ubiquitin ligase; Ligase; EC 6.3.2.19; EC 6.3.2.-; Adaptor/scaffold; Ubiquitin conjugating system
Cellular Component: cytosol
Molecular Function: protein binding; ribosomal S6-glutamic acid ligase activity; ubiquitin-protein ligase activity; ATP binding; NEDD8 ligase activity
Biological Process: protein neddylation; positive regulation of neuron apoptosis; transforming growth factor beta receptor signaling pathway; protein ubiquitination; protein modification process
Reference #:  P61081 (UniProtKB)
Alt. Names/Synonyms: hUbc12; NEDD8 carrier protein; NEDD8 protein ligase; NEDD8-conjugating enzyme Ubc12; UBC-RS2; UBC12; UBE2M; ubiquitin carrier protein M; Ubiquitin-conjugating enzyme E2 M; ubiquitin-conjugating enzyme E2M; ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast); ubiquitin-protein ligase M; yeast UBC12 homolog
Gene Symbols: UBE2M
Molecular weight: 20,900 Da
Basal Isoelectric point: 7.57  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

UBE2M

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 K3-a _____MIkLFSLkQQ
0 2 K3-u _____MIkLFSLkQQ
0 12 K8-u MIkLFSLkQQKKEEE
0 1 T20-p EEESAGGtKGsSKKA
0 3 S23-p SAGGtKGsSKKAsAA
0 3 S28-p KGsSKKAsAAQLRIQ
0 2 K36-u AAQLRIQkDINELNL
0 23 K45-u INELNLPkTCDIsFS
0 1 S50-p LPkTCDIsFSDPDDL
0 2 K61-u PDDLLNFkLVICPDE
0 8 K72-u CPDEGFYkSGkFVFS
0 9 K75-u EGFYkSGkFVFSFkV
0 1 K81-u GkFVFSFkVGQGyPH
0 1 Y86-p SFkVGQGyPHDPPkV
0 14 K92-u GyPHDPPkVkCETMV
0 4 K94-u PHDPPkVkCETMVYH
0 30 R169-m1 QNVQRSMrGGyIGST
0 1 R169 QNVQRSMRGGyIGST
0 3 Y172-p QRSMrGGyIGSTyFE
0 9 Y177-p GGyIGSTyFERCLK_
  mouse

 
K3 _____MIKLFSLkQQ
K3-u _____MIkLFSLkQQ
K8-u MIkLFSLkQQKKEEE
T20 EEESAGGTKGSSKKA
S23 SAGGTKGSSKKAsAA
S28-p KGSSKKAsAAQLRIQ
K36-u AAQLRIQkDINELNL
K45-u INELNLPkTCDISFS
S50 LPkTCDISFSDPDDL
K61 PDDLLNFKLVICPDE
K72-u CPDEGFYkSGkFVFS
K75-u EGFYkSGkFVFSFKV
K81 GkFVFSFKVGQGYPH
Y86 SFKVGQGYPHDPPkV
K92-u GYPHDPPkVKCETMV
K94 PHDPPkVKCETMVYH
R169-m1 QNVQRSMrGGYIGST
R169-m2 QNVQRSMrGGYIGST
Y172 QRSMrGGYIGSTYFE
Y177 GGYIGSTYFERCLK_
  rat

 
K3 _____MIKLFSLkQQ
K3 _____MIKLFSLkQQ
K8-u MIKLFSLkQQKKEEE
T20 EEESAGGTKGSSKKA
S23 SAGGTKGSSKKASAA
S28 KGSSKKASAAQLRIQ
K36 AAQLRIQKDINELNL
K45 INELNLPKTCDISFS
S50 LPKTCDISFSDPDDL
K61 PDDLLNFKLVICPDE
K72 CPDEGFYKSGKFVFS
K75 EGFYKSGKFVFSFKV
K81 GKFVFSFKVGQGYPH
Y86 SFKVGQGYPHDPPKV
K92 GYPHDPPKVKCETMV
K94 PHDPPKVKCETMVYH
R169-m1 QNVQRSMrGGYIGST
R169 QNVQRSMRGGYIGST
Y172 QRSMrGGYIGSTYFE
Y177 GGYIGSTYFERCLK_
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