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PRMT5 (human)

Overview PRMT5 an ubiquitous enzyme with protein methyltransferase activity. Methylates specific arginine residues in the transcription elongation factor SPT5, and the small nuclear ribonucleoproteins SNRPD1 and SNRPD3. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SPT5 transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Component of the methylosome, a 20S complex that includes ICLN, SKB1 and WDR77. Component of a high molecular weight E2F-pocket protein complex, CERC (cyclin E1 repressor complex). Also interacts with Sm proteins, JAK2, SSTR1 and SPT5. Associates with SWI/SNF remodeling complexes containing SMARCA2 and SMARCA4. Note: This description may include information from UniProtKB. Protein type: Methyltransferase, protein arginine; RNA processing; EC 2.1.1.-; Cell cycle regulation; Methyltransferase; EC 2.1.1.125 Cellular Component: protein complex; cytoplasm; cytosol; nucleus Molecular Function: methyltransferase activity; ribonucleoprotein binding; protein binding; protein-arginine omega-N symmetric methyltransferase activity; histone-arginine N-methyltransferase activity; chromatin binding Biological Process: cell proliferation; peptidyl-arginine methylation, to symmetrical-dimethyl arginine; peptidyl-arginine methylation; spliceosomal snRNP biogenesis; transcription, DNA-dependent; RNA metabolic process; regulation of mitosis; gene expression; negative regulation of transcription from RNA polymerase II promoter; peptidyl-arginine N-methylation; endothelial cell activation Reference #:  O14744 (UniProtKB) Alt. Names/Synonyms: 72 kDa ICln-binding protein; ANM5; Histone-arginine N-methyltransferase PRMT5; HMT1 hnRNP methyltransferase-like 5; HRMT1L5; IBP72; Jak-binding protein 1; JBP1; PRMT5; protein arginine methyltransferase 5; Protein arginine N-methyltransferase 5; Shk1 kinase-binding protein 1 homolog; SKB1; SKB1 homolog; SKB1Hs Gene Symbols: PRMT5 Molecular weight: 72,684 Da Basal Isoelectric point: 5.88  Predict pI for various phosphorylation states CST Pathways:  Histone Methylation Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below PRMT5 4GQB - A=1-637 (human)

STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB  |  ENZYME 2.1.1.- 2.1.1.125  |  DISEASE  |  Source  |  InnateDB  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene

	Sites Implicated In
carcinogenesis, altered: Y297‑p, Y304‑p, Y307‑p cell growth, altered: Y297‑p, Y304‑p, Y307‑p enzymatic activity, inhibited: Y297‑p, Y304‑p, Y307‑p molecular association, regulation: Y297‑p, Y304‑p, Y307‑p

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
0	1		K60-u	EFIQEPAkNRPGPQT
0	1		S74-p	TRSDLLLsGRDWNTL
0	3		K85-u	WNTLIVGkLSPWIRP
0	13		K95-u	PWIRPDSkVEKIRRN
0	1		Y179-p	PTTHTEEySGEEKTW
0	1		K200-a	RTLCDYSkRIAVALE
0	42		K227-u	RWLGEPIkAAILPTS
0	4		K240-u	TSIFLTNkKGFPVLS
0	1		K241	SIFLTNkKGFPVLSK
0	1		S279-p	HSEKEFCsyLQyLEy
0	20		Y280-p	SEKEFCsyLQyLEyL
0	8		Y283-p	EFCsyLQyLEyLsQN
0	23		Y286-p	syLQyLEyLsQNRPP
0	1		S288-p	LQyLEyLsQNRPPPN
1	0		Y297-p	NRPPPNAyELFAKGy
1	0		Y304-p	yELFAKGyEDyLQsP
1	0		Y307-p	FAKGyEDyLQsPLQP
0	8		S310-p	GyEDyLQsPLQPLMD
0	1		K333-u	VFEKDPIkYSQYQQA
0	72		Y342-p	SQYQQAIyKCLLDRV
0	1		K387-u	KQADRRIkLYAVEKN
0	1		K479-u	LAPISSSkLYNEVRA
0	1		K490-u	EVRACREkDRDPEAQ

	mouse
K60	EFIQEPAKNRPGPQT
S74	TRSDLLLSGRDWNTL
K85	WNTLIVGKLSPWIHP
K95-u	PWIHPDSkVEKIRRN
Y179	PTTHTEEYSGEEKTW
K200	RTLCDYSKRIAVALE
K227-u	RWLGEPIkAAILPTS
K240-u	TSIFLTNkkGFPVLS
K241-u	SIFLTNkkGFPVLSK
S279	HSEKEFCSYLQYLEY
Y280	SEKEFCSYLQYLEYL
Y283	EFCSYLQYLEYLSQN
Y286	SYLQYLEYLSQNRPP
S288	LQYLEYLSQNRPPPN
Y297	NRPPPNAYELFAKGY
Y304	YELFAKGYEDYLQSP
Y307	FAKGYEDYLQSPLQP
S310	GYEDYLQSPLQPLMD
K333	VFEKDPIKYSQYQQA
Y342	SQYQQAIYKCLLDRV
R387	KQAERRIRLYAVEKN
K479	LAPISSSKLYNEVRA
K490	EVRACREKDRDPEAQ

	rat
K60	EFIQEPAKNRPGPQT
S74	TRSDLLLSGRDWNTL
K85	WNTLIVGKLSPWIHP
K95-u	PWIHPDSkVEKIRRN
Y179	PTAHTEEYSGEEKTW
K200	RTLCDYSKRIAVALE
K227	RWLGEPIKAAILPTS
K240	TSIFLTNKKGFPVLS
K241	SIFLTNKKGFPVLSK
S279	HSEKEFCSYLQYLEY
Y280	SEKEFCSYLQYLEYL
Y283	EFCSYLQYLEYLSQN
Y286	SYLQYLEYLSQNRPP
S288	LQYLEYLSQNRPPPN
Y297	NRPPPNAYELFAKGY
Y304	YELFAKGYEDYLQSP
Y307	FAKGYEDYLQSPLQP
S310	GYEDYLQSPLQPLMD
K333	VFEKDPIKYSQYQQA
Y342	SQYQQAIYKCLLDRV
R387	KQAERRIRLYAVEKN
K479	LAPISSSKLYNEVRA
K490	EVRACREKDRDPEAQ

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