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Protein Page:
PRMT5 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
PRMT5 an ubiquitous enzyme with protein methyltransferase activity. Methylates specific arginine residues in the transcription elongation factor SPT5, and the small nuclear ribonucleoproteins SNRPD1 and SNRPD3. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SPT5 transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Component of the methylosome, a 20S complex that includes ICLN, SKB1 and WDR77. Component of a high molecular weight E2F-pocket protein complex, CERC (cyclin E1 repressor complex). Also interacts with Sm proteins, JAK2, SSTR1 and SPT5. Associates with SWI/SNF remodeling complexes containing SMARCA2 and SMARCA4. Note: This description may include information from UniProtKB.
Protein type: EC 2.1.1.-; Cell cycle regulation; RNA processing; Methyltransferase; Methyltransferase, protein arginine; EC 2.1.1.125
Cellular Component: cytoplasm; nucleus; cytosol
Molecular Function: ribonucleoprotein binding; methyltransferase activity; protein binding; protein-arginine omega-N symmetric methyltransferase activity; histone-arginine N-methyltransferase activity; chromatin binding
Biological Process: cell proliferation; peptidyl-arginine methylation, to symmetrical-dimethyl arginine; spliceosomal snRNP biogenesis; peptidyl-arginine methylation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; RNA metabolic process; regulation of mitosis; gene expression; negative regulation of transcription from RNA polymerase II promoter; peptidyl-arginine N-methylation; endothelial cell activation
Reference #:  O14744 (UniProtKB)
Alt. Names/Synonyms: 72 kDa ICln-binding protein; ANM5; Histone-arginine N-methyltransferase PRMT5; HMT1 hnRNP methyltransferase-like 5; HRMT1L5; IBP72; Jak-binding protein 1; JBP1; PRMT5; protein arginine methyltransferase 5; Protein arginine N-methyltransferase 5; Shk1 kinase-binding protein 1 homolog; SKB1; SKB1 homolog; SKB1Hs
Gene Symbols: PRMT5
Molecular weight: 72,684 Da
Basal Isoelectric point: 5.88  Predict pI for various phosphorylation states
CST Pathways:  Histone Methylation
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PRMT5

Protein Structure Not Found.


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Sites Implicated In
carcinogenesis, altered: Y297‑p, Y304‑p, Y307‑p
cell growth, altered: Y297‑p, Y304‑p, Y307‑p
enzymatic activity, inhibited: Y297‑p, Y304‑p, Y307‑p
molecular association, regulation: Y297‑p, Y304‑p, Y307‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 K60-u EFIQEPAkNRPGPQT
0 3 K85-u WNTLIVGkLSPWIRP
0 13 K95-u PWIRPDSkVEKIRRN
0 1 K200-a RTLCDYSkRIAVALE
0 42 K227-u RWLGEPIkAAILPTS
0 4 K240-u TSIFLTNkKGFPVLS
0 20 Y280-p SEKEFCSyLQyLEyL
0 8 Y283-p EFCSyLQyLEyLSQN
0 23 Y286-p SyLQyLEyLSQNRPP
1 0 Y297-p NRPPPNAyELFAKGy
1 0 Y304-p yELFAKGyEDyLQsP
1 0 Y307-p FAKGyEDyLQsPLQP
0 8 S310-p GyEDyLQsPLQPLMD
0 1 K333-u VFEKDPIkYSQYQQA
0 72 Y342-p SQYQQAIyKCLLDRV
0 1 K387-u KQADRRIkLYAVEKN
0 1 K479-u LAPISSSkLYNEVRA
0 1 K490-u EVRACREkDRDPEAQ
  mouse

 
K60 EFIQEPAKNRPGPQT
K85 WNTLIVGKLSPWIHP
K95-u PWIHPDSkVEKIRRN
K200 RTLCDYSKRIAVALE
K227-u RWLGEPIkAAILPTS
K240-u TSIFLTNkKGFPVLS
Y280 SEKEFCSYLQYLEYL
Y283 EFCSYLQYLEYLSQN
Y286 SYLQYLEYLSQNRPP
Y297 NRPPPNAYELFAKGY
Y304 YELFAKGYEDYLQSP
Y307 FAKGYEDYLQSPLQP
S310 GYEDYLQSPLQPLMD
K333 VFEKDPIKYSQYQQA
Y342 SQYQQAIYKCLLDRV
R387 KQAERRIRLYAVEKN
K479 LAPISSSKLYNEVRA
K490 EVRACREKDRDPEAQ
  rat

 
K60 EFIQEPAKNRPGPQT
K85 WNTLIVGKLSPWIHP
K95-u PWIHPDSkVEKIRRN
K200 RTLCDYSKRIAVALE
K227 RWLGEPIKAAILPTS
K240 TSIFLTNKKGFPVLS
Y280 SEKEFCSYLQYLEYL
Y283 EFCSYLQYLEYLSQN
Y286 SYLQYLEYLSQNRPP
Y297 NRPPPNAYELFAKGY
Y304 YELFAKGYEDYLQSP
Y307 FAKGYEDYLQSPLQP
S310 GYEDYLQSPLQPLMD
K333 VFEKDPIKYSQYQQA
Y342 SQYQQAIYKCLLDRV
R387 KQAERRIRLYAVEKN
K479 LAPISSSKLYNEVRA
K490 EVRACREKDRDPEAQ
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