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Protein Page:
CASK (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
CASK a MAGUK family protein and a protein kinase of the CAMK group. Contains one protein kinase domain, one guanylate kinase domain, two L27 domains, one PDZ domain and one SH3 domain. Plays a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1. Lacks the canonical DFG motif required for Mg++ binding, but retains protein kinase activity even without binding Mg++. Six alternatively spliced human isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, Ser/Thr (non-receptor); Adaptor/scaffold; Kinase, protein; Protein kinase, CAMK; EC 2.7.11.1; CAMK group; CASK family
Cellular Component: presynaptic membrane; nuclear lamina; nuclear matrix; basolateral plasma membrane; cytoplasm; nucleolus; plasma membrane; basement membrane; intercellular junction; cytosol; actin cytoskeleton
Molecular Function: calmodulin binding; guanylate kinase activity; protein serine/threonine kinase activity; protein binding; neurexin binding; ATP binding
Biological Process: extracellular matrix organization and biogenesis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell-matrix adhesion; cell adhesion; protein amino acid phosphorylation; nucleotide phosphorylation
Reference #:  O14936 (UniProtKB)
Alt. Names/Synonyms: CAGH39; Calcium/calmodulin-dependent serine protein kinase; calcium/calmodulin-dependent serine protein kinase (MAGUK family); calcium/calmodulin-dependent serine protein kinase membrane-associated guanylate kinase; CAMGUK; CASK; CMG; CSKP; FGS4; FLJ22219; FLJ31914; hCASK; LIN2; MICPCH; Peripheral plasma membrane protein CASK; Protein lin-2 homolog; TNRC8; trinucleotide repeat containing 8
Gene Symbols: CASK
Molecular weight: 105,123 Da
Basal Isoelectric point: 5.99  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

CASK

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 K41 TGQQFAVKIVDVAKF
0 2 S50 VDVAKFTSsPGLSTE
1 3 S51-p DVAKFTSsPGLSTED
0 1 K60-ub GLSTEDLkREASICH
0 1 S83 ELLETYSSDGMLYMV
0 1 Y88 YSSDGMLYMVFEFMD
0 1 Y113 RADAGFVYSEAVASH
0 1 S119 VYSEAVASHYMRQIL
0 1 Y121 SEAVASHYMRQILEA
1 0 S151-p PHCVLLAsKENsAPV
1 0 S155-p LLAsKENsAPVKLGG
0 3 T182-p VAGGRVGtPHFMAPE
0 1 Y282-p WLKERDRyAYKIHLP
0 3 S313-p GAVLAAVssHKFNSF
0 1 S314-p AVLAAVssHKFNSFY
0 1 S319 VssHKFNSFYGDPPE
0 2 S348 LAAERAVSQVLDSLE
0 2 S353 AVSQVLDSLEEIHAL
0 6 Y387-p LHTLLDLyDKINTKS
0 1 K393 LyDKINTKSSPQIRN
1 1 S395 DKINTKSSPQIRNPP
0 1 K410 SDAVQRAKEVLEEIS
0 1 T433 KELKRILTQPHFMAL
0 1 T460-p SDEALRVtPPPTsPY
0 2 S465-p RVtPPPTsPYLNGDS
0 1 K554 QTVEQLQKMLREMRG
1 0 S562-p MLREMRGsITFKIVP
0 1 S570-p ITFKIVPsyRtQSSS
0 1 Y571-p TFKIVPsyRtQSSSC
0 1 T573-p KIVPsyRtQSSSCER
0 3 S575 VPsyRtQSSSCERDs
0 5 S576 PsyRtQSSSCERDsP
0 15 S577 syRtQSSSCERDsPS
0 1 S582-p SSSCERDsPSTSRQS
0 1 S650 GDIIQIISKDDHNWW
0 2 K665-ub QGKLENSkNGTAGLI
1 0 K691 CIAMEKTKQEQQASC
0 1 K691 CIAMEKTKQEQQASC
0 2 Y708-p FGKKKKQyKDKYLAK
1 2 T741-p LPAFKRKtLVLLGAH
0 1 K762 IKNTLITKHPDRFAy
0 2 Y769-p KHPDRFAyPIPHTtR
0 1 T774 FAyPIPHTtRPPKKD
0 2 T775-p AyPIPHTtRPPKKDE
0 5 Y788-p DEENGKNyyFVsHDQ
0 2 Y789-p EENGKNyyFVsHDQM
0 1 S792-p GKNyyFVsHDQMMQD
0 4 K825-ub TKLETIRkIHEQGLI
  CASK iso2  
K41 TGQQFAVKIVDVAKF
S50 VDVAKFTSSPGLSTE
S51 DVAKFTSSPGLSTED
K60 GLSTEDLKREASICH
S83 ELLETYSSDGMLYMV
Y88 YSSDGMLYMVFEFMD
Y113 RADAGFVYSEAVASH
S119 VYSEAVASHYMRQIL
Y121 SEAVASHYMRQILEA
S151 PHCVLLASKENSAPV
S155 LLASKENSAPVKLGG
T182 VAGGRVGTPHFMAPE
Y282 WLKERDRYAYKIHLP
S313 GAVLAAVSSHKFNSF
S314 AVLAAVSSHKFNSFY
S319 VSSHKFNSFYGDPPE
S348 LAAERAVSQVLDSLE
S353 AVSQVLDSLEEIHAL
Y387 LHTLLDLYDKINTKS
K393 LYDKINTKSSPQIRN
S395 DKINTKSSPQIRNPP
K410 SDAVQRAKEVLEEIS
T433 KELKRILTQPHFMAL
T460 SDEALRVTPPPTSPY
S465 RVTPPPTSPYLNGDS
K554 QTVEQLQKMLREMRG
S562 MLREMRGSITFKIVP
S570 ITFKIVPSYRTQSSS
Y571 TFKIVPSYRTQSSSC
T573 KIVPSYRTQSSSCER
S575 VPSYRTQSSSCERDS
S576 PSYRTQSSSCERDSP
S577 SYRTQSSSCERDSPS
S582 SSSCERDSPSTSRQS
S650 GDIIQIISKDDHNWW
K665 QGKLENSKNGTAGLI
K691 CIAMEKTKQEQQASC
K691 CIAMEKTKQEQQASC
Y708 FGKKKKQYKDKYLAK
T736 LPAFKRKTLVLLGAH
K757 IKNTLITKHPDRFAY
Y764 KHPDRFAYPIPHTTR
T769 FAYPIPHTTRPPKKD
T770 AYPIPHTTRPPKKDE
Y783 DEENGKNYYFVSHDQ
Y784 EENGKNYYFVSHDQM
S787 GKNYYFVSHDQMMQD
K820 TKLETIRKIHEQGLI
  CASK iso3  
K41 TGQQFAVKIVDVAKF
S50 VDVAKFTSSPGLSTE
S51 DVAKFTSSPGLSTED
K60 GLSTEDLKREASICH
S83 ELLETYSSDGMLYMV
Y88 YSSDGMLYMVFEFMD
Y113 RADAGFVYSEAVASH
S119 VYSEAVASHYMRQIL
Y121 SEAVASHYMRQILEA
S151 PHCVLLASKENSAPV
S155 LLASKENSAPVKLGG
T182 VAGGRVGTPHFMAPE
Y282 WLKERDRYAYKIHLP
S313 GAVLAAVSSHKFNsF
S314 AVLAAVSSHKFNsFY
S319-p VSSHKFNsFYGDPPE
S342 PTSSGAVSQVLDSLE
S347 AVSQVLDSLEEIHAL
Y381 LHTLLDLYDKINTKS
K387 LYDKINTKSSPQIRN
S389 DKINTKSSPQIRNPP
K404 SDAVQRAKEVLEEIS
T427 KELKRILTQPHFMAL
T454 SDEALRVTPPPTSPY
S459 RVTPPPTSPYLNGDS
K548 QTVEQLQKMLREMRG
S556 MLREMRGSITFKIVP
S564 ITFKIVPSYRTQsss
Y565 TFKIVPSYRTQsssC
T567 KIVPSYRTQsssCED
S569-p VPSYRTQsssCEDLP
S570-p PSYRTQsssCEDLPS
S571-p SYRTQsssCEDLPST
- gap
S621 GDIIQIISKDDHNWW
K636 QGKLENSKNGTAGLI
K662 CIAMEKTKQEQQASC
K662 CIAMEKTKQEQQASC
Y679 FGKKKKQYKDKYLAK
T712 LPAFKRKTLVLLGAH
K733 IKNTLITKHPDRFAY
Y740 KHPDRFAYPIPHTTR
T745 FAYPIPHTTRPPKKD
T746 AYPIPHTTRPPKKDE
Y759 DEENGKNYYFVSHDQ
Y760 EENGKNYYFVSHDQM
S763 GKNYYFVSHDQMMQD
K796 TKLETIRKIHEQGLI
  mouse

► Hide Isoforms
 
K41-ub TGQQFAVkIVDVAKF
S50-p VDVAKFTssPGLSTE
S51-p DVAKFTssPGLSTED
K60 GLSTEDLKREASICH
S83-p ELLETYSsDGMLyMV
Y88-p YSsDGMLyMVFEFMD
Y113-p RADAGFVySEAVAsH
S119-p VySEAVAsHyMRQIL
Y121-p SEAVAsHyMRQILEA
S151 PHCVLLASKENSAPV
S155 LLASKENSAPVKLGG
T182-p VAGGRVGtPHFMAPE
Y282 WLKERDRYAYKIHLP
S313-p GAVLAAVsSHKFNSF
S314 AVLAAVsSHKFNSFY
S319 VsSHKFNSFYGDPPE
S348-p LAAERAVsQVLDsLE
S353-p AVsQVLDsLEEIHAL
Y387 LHTLLDLYDKINTkS
K393-ub LYDKINTkSsPQIRN
S395-p DKINTkSsPQIRNPP
K410-ub SDAVQRAkEVLEEIS
T433-p KELKRILtQPHFMAL
T460 SDEALRVTPPPTSPY
S465 RVTPPPTSPYLNGDS
K554-ub QTVEQLQkMLREMRG
S562 MLREMRGSITFKIVP
S570 ITFKIVPSYRTQSSs
Y571 TFKIVPSYRTQSSsC
T573 KIVPSYRTQSSsCER
S575 VPSYRTQSSsCERDs
S576 PSYRTQSSsCERDsP
S577-p SYRTQSSsCERDsPS
S582-p SSsCERDsPSTSRQS
S650-p GDIIQIIsKDDHNWW
K665-ub QGKLENSkNGTAGLI
K691 CIAMEKTKQEQQASC
K691-ub CIAMEKTkQEQQASC
Y708-p FGKKKKQyKDKYLAK
T741 LPAFKRKTLVLLGAH
K762-ub IKNTLITkHPDRFAy
Y769-p kHPDRFAyPIPHttR
T774-p FAyPIPHttRPPKKD
T775-p AyPIPHttRPPKKDE
Y788 DEENGKNYYFVSHDQ
Y789 EENGKNYYFVSHDQM
S792 GKNYYFVSHDQMMQD
K825-ub TKLETIRkIHEQGLI
  CASK iso3  
K41 TGQQFAVKIVDVAKF
S50 VDVAKFTSSPGLSTE
S51 DVAKFTSSPGLSTED
K60 GLSTEDLKREASICH
S83 ELLETYSSDGMLYMV
Y88 YSSDGMLYMVFEFMD
Y113 RADAGFVYSEAVASH
S119 VYSEAVASHYMRQIL
Y121 SEAVASHYMRQILEA
S151 PHCVLLASKENSAPV
S155 LLASKENSAPVKLGG
T182 VAGGRVGTPHFMAPE
Y282 WLKERDRYAYKIHLP
S313 GAVLAAVSSHKFNSF
S314 AVLAAVSSHKFNSFY
S319 VSSHKFNSFYGDPPE
S342 PTSSGAVSQVLDSLE
S347 AVSQVLDSLEEIHAL
Y381 LHTLLDLYDKINTKS
K387 LYDKINTKSSPQIRN
S389 DKINTKSSPQIRNPP
K404 SDAVQRAKEVLEEIS
T427 KELKRILTQPHFMAL
T454 SDEALRVTPPPTSPY
S459 RVTPPPTSPYLNGDS
K548 QTVEQLQKMLREMRG
S556 MLREMRGSITFKIVP
S564 ITFKIVPSYRTQsss
Y565 TFKIVPSYRTQsssC
T567 KIVPSYRTQsssCED
S569-p VPSYRTQsssCEDLP
S570-p PSYRTQsssCEDLPS
S571-p SYRTQsssCEDLPST
- gap
S621 GDIIQIISKDDHNWW
K636 QGKLENSKNGTAGLI
K662 CIAMEKTKQEQQASC
K662 CIAMEKTKQEQQASC
Y679 FGKKKKQYKDKYLAK
T712 LPAFKRKTLVLLGAH
K733 IKNTLITKHPDRFAY
Y740 KHPDRFAYPIPHTTR
T745 FAYPIPHTTRPPKKD
T746 AYPIPHTTRPPKKDE
Y759 DEENGKNYYFVSHDQ
Y760 EENGKNYYFVSHDQM
S763 GKNYYFVSHDQMMQD
K796 TKLETIRKIHEQGLI
  rat

 
K41 TGQQFAVKIVDVAKF
S50 VDVAKFTSSPGLSTE
S51 DVAKFTSSPGLSTED
K60 GLSTEDLKREASICH
S83 ELLETYSSDGMLYMV
Y88 YSSDGMLYMVFEFMD
Y113 RADAGFVYSEAVASH
S119 VYSEAVASHYMRQIL
Y121 SEAVASHYMRQILEA
S151 PHCVLLASKENSAPV
S155 LLASKENSAPVKLGG
T182 VAGGRVGTPHFMAPE
Y282 WLKERDRYAYKIHLP
S313 GAVLAAVSSHKFNSF
S314 AVLAAVSSHKFNSFY
S319 VSSHKFNSFYGDPPE
S348 LAAERAVSQVLDSLE
S353 AVSQVLDSLEEIHAL
Y387 LHTLLDLYDKINTKS
K393 LYDKINTKSSPQIRN
S395 DKINTKSSPQIRNPP
K410 SDAVQRAKEVLEEIS
T433 KELKRILTQPHFMAL
T460 SDEALRVTPPPTSPY
S465 RVTPPPTSPYLNGDS
K554 QTVEQLQKMLREMRG
S562-p MLREMRGsITFKIVP
S570 ITFKIVPSYRTQSSS
Y571 TFKIVPSYRTQSSSC
T573 KIVPSYRTQSSSCER
S575 VPSYRTQSSSCERDS
S576 PSYRTQSSSCERDSP
S577 SYRTQSSSCERDSPS
S582 SSSCERDSPSTSRQS
S638 GDIIQIISKDDHNWW
K653 QGKLENSKNGTAGLI
K679-sm CIAMEKTkQEQQASC
K679 CIAMEKTKQEQQASC
Y696 FGKKKKQYKDKYLAK
T724-p LPAFKRKtLVLLGAH
K745 IKNTLITKHPERFAY
Y752 KHPERFAYPIPHTTR
T757 FAYPIPHTTRPPKKD
T758 AYPIPHTTRPPKKDE
Y771 DEENGKNYYFVSHDQ
Y772 EENGKNYYFVSHDQM
S775 GKNYYFVSHDQMMQD
K808 TKLETIRKIHEQGLI
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