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Protein Page:
PRP4 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PRP4 a kinase of the DYRK family associated with the spliceosome and the kinetochore. It binds pre-mRNA splicing factors SFRS8, PRP6 and pinin. It is an essential kinase that associates with both the U5 snRNP and the N-CoR deacetylase complexes. It may help coordinate pre-mRNA splicing with chromatin remodeling and transcriptional regulation. PRP4 has recently been shown to be a kinetochore component that is necessary to establish a functional spindle assembly checkpoint, and is necessary for recruitment or maintenance of the checkpoint proteins RPS27, MAD1, and MAD2 at the kinetochores. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, CMGC; RNA splicing; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Spliceosome; EC 2.7.11.1; CMGC group; DYRK family; PRP4 subfamily
Cellular Component: nucleolus; chromosome; nucleus
Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity
Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; protein amino acid phosphorylation
Reference #:  Q13523 (UniProtKB)
Alt. Names/Synonyms: dJ1013A10.1; dJ1013A10.1 (PRP4 protein kinase homolog); KIAA0536; PR4H; PRP4; PRP4 kinase; PRP4 pre-mRNA processing factor 4 homolog B (yeast); PRP4 pre-mRNA-processing factor 4 homolog; PRP4B; PRP4H; PRP4K; PRPF4B; Serine/threonine-protein kinase PRP4 homolog; serine/threonine-protein kinase PRP4K
Gene Symbols: PRPF4B
Molecular weight: 116,987 Da
Basal Isoelectric point: 10.26  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PRP4

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S8 MAAAETQSLREQPEM
0 33 S20-p PEMEDANsEKsINEE
0 32 S23-p EDANsEKsINEENGE
0 29 S32-p NEENGEVsEDQsQNK
0 11 S36-p GEVsEDQsQNKHsRH
0 1 S41-p DQsQNKHsRHKKKKH
0 29 S87-p RKEIIDAsDKEGMsP
0 30 S93-p AsDKEGMsPAKRTKL
0 1 K111 ALLEDLEKQRALIKA
0 1 S131-p LMEGKVQsGMGLILQ
0 10 Y140-p MGLILQGyEsGsEEE
0 32 S142-p LILQGyEsGsEEEGE
0 40 S144-p LQGyEsGsEEEGEIH
0 4 T162-p RNGNRSStRsssTkG
0 23 S164-p GNRSStRsssTkGKL
0 17 S165-p NRSStRsssTkGKLE
0 21 S166-p RSStRsssTkGKLEL
0 3 T167 SStRsssTkGKLELV
0 1 K168-ac StRsssTkGKLELVD
0 1 K177-ac KLELVDNkITTKkRs
0 1 K182-ub DNkITTKkRsKSRSK
0 2 S184-p kITTKkRsKSRSKER
0 6 S196-p KERTRHRsDKKkskG
0 1 K200-ac RHRsDKKkskGGIEI
0 1 S201-p HRsDKKkskGGIEIV
0 1 K202-ac RsDKKkskGGIEIVK
0 2 S232 SPSKRSKSQDQARKs
0 28 S239-p SQDQARKsKsPtLRR
0 27 S241-p DQARKsKsPtLRRRS
0 2 T243-p ARKsKsPtLRRRSQE
0 13 S248 sPtLRRRSQEKIGKA
0 13 S257-p EKIGKARsPtDDKVK
0 3 T259-p IGKARsPtDDKVKIE
0 83 S277-p KSKDRKKsPIINEsR
0 4 S283-p KsPIINEsRsRDRGK
0 1 S285-p PIINEsRsRDRGKKs
0 29 S292-p sRDRGKKsRsPVDLR
0 31 S294-p DRGKKsRsPVDLRGK
0 19 S328-p KEKKPIKsPsKDASs
0 6 S330-p KKPIKsPsKDASsGK
0 1 S335-p sPsKDASsGKENRSP
0 2 S349-p PSRRPGRsPKRRsLs
0 15 S354-p GRsPKRRsLsPkPRD
0 14 S356-p sPKRRsLsPkPRDKS
0 1 K358-ac KRRsLsPkPRDKSRR
0 27 S366-p PRDKSRRsRsPLLND
0 25 S368-p DKSRRsRsPLLNDRR
0 5 S381-p RRSKQSKsPsRtLsP
0 4 S383-p SKQSKsPsRtLsPGR
0 5 T385-p QSKsPsRtLsPGRRA
0 16 S387-p KsPsRtLsPGRRAKs
0 3 S394-p sPGRRAKsRsLERKR
0 3 S396-p GRRAKsRsLERKRRE
0 5 S410-p EPERRRLssPRtRPR
0 6 S411-p PERRRLssPRtRPRD
0 2 T414-p RRLssPRtRPRDDIL
0 13 S427-p ILSRRERsKDAsPIN
0 31 S431-p RERsKDAsPINRWsP
0 23 S437-p AsPINRWsPtRRRsR
0 4 T439-p PINRWsPtRRRsRsP
0 3 S443-p WsPtRRRsRsPIRRR
0 3 S445-p PtRRRsRsPIRRRSR
0 3 S458-p SRSPLRRsRsPRRRS
0 3 S460-p SPLRRsRsPRRRSRS
0 1 S507-p KEDKFKGsLsEGMKV
0 1 S509-p DKFKGsLsEGMKVEQ
0 9 S518-p GMKVEQEsssDDNLE
0 9 S519-p MKVEQEsssDDNLED
0 8 S520-p KVEQEsssDDNLEDF
0 1 Y552 RQAIVQKYKyLAEDS
0 2 Y554-p AIVQKYKyLAEDSNM
0 1 S562-p LAEDSNMsVPsEPss
0 3 S565-p DSNMsVPsEPssPQS
0 1 S568-p MsVPsEPssPQSStR
0 4 S569-p sVPsEPssPQSStRt
0 2 T574-p PssPQSStRtRsPsP
0 16 T576-p sPQSStRtRsPsPDD
0 28 S578-p QSStRtRsPsPDDIL
0 30 S580-p StRtRsPsPDDILER
0 1 S622-p TVEQNNGsSQKKLLA
0 1 S623 VEQNNGsSQKKLLAP
0 2 S636-p APDMFTEsDDMFAAy
0 15 Y643-p sDDMFAAyFDSARLR
0 2 K656-ac LRAAGIGkDFKENPN
0 1 Y674-p NWTDAEGyYRVNIGE
0 1 K717-ac ANQEVAVkIIRNNEL
0 1 K737 LKELEFLKKLNDADP
0 2 S794-p LHIKAVRsYSQQLFL
0 1 Y795 HIKAVRsYSQQLFLA
0 43 S837-p LKLCDFGsAsHVADN
0 14 S839-p LCDFGsAsHVADNDI
0 18 T847-p HVADNDItPyLVsRF
2 4018 Y849-p ADNDItPyLVsRFyR
0 424 S852-p DItPyLVsRFyRAPE
0 3 Y855-p PyLVsRFyRAPEIII
0 1 K905-ac LKLAMDLkGkMPNKM
0 1 K907-ac LAMDLkGkMPNKMIR
  mouse

 
S8-p MAATEPPsLREQAEM
S21-p EMDDADNsEKsVNEE
S24-p DADNsEKsVNEENGE
S33-p NEENGEVsEDQsQNK
S37-p GEVsEDQsQNKHSRH
S42 DQsQNKHSRHKKKKH
S88-p RKEVIEAsDKEGLsP
S94-p AsDKEGLsPAKRTKL
K112-ub ALLEDLEkQRALIKA
S132 LMEGKVQSGMGLILQ
Y141-p MGLILQGyEsGsEEE
S143-p LILQGyEsGsEEEGE
S145-p LQGyEsGsEEEGEIH
T163 RNGNRSSTRssstRG
S165-p GNRSSTRssstRGKL
S166-p NRSSTRssstRGKLE
S167-p RSSTRssstRGKLEI
T168-p SSTRssstRGKLEIT
R169 STRssstRGKLEITD
K178 KLEITDNKNSAKKRS
K183 DNKNSAKKRSKSRSK
S185 KNSAKKRSKSRSKER
S197-p KERTRHRsDKRKSKG
K201 RHRsDKRKSKGAGEM
S202 HRsDKRKSKGAGEML
K203 RsDKRKSKGAGEMLR
S233-p SPSKRSKsQDQARKs
S240-p sQDQARKsKsPPLRR
S242-p DQARKsKsPPLRRRs
P244 ARKsKsPPLRRRsQE
S249-p sPPLRRRsQEKVGKA
S258-p EKVGKARsPAEEKMK
A260 VGKARsPAEEKMKSE
S278-p KIKDRKKsPIVNERS
- gap
S285 sPIVNERSRDRSKKs
S292-p SRDRSKKsKsPVDLR
S294-p DRSKKsKsPVDLRDK
S328-p KEKKPIKsPsKDASS
S330-p KKPIKsPsKDASSGK
S335 sPsKDASSGKENRSP
S349 PSRRPGRSPKRRsLs
S354-p GRSPKRRsLsPKLRD
S356-p SPKRRsLsPKLRDKS
K358 KRRsLsPKLRDKSRR
S366-p LRDKSRRsRsPLLND
S368-p DKSRRsRsPLLNDRR
S381-p RRSKQSKsPsRtLsP
S383-p SKQSKsPsRtLsPGR
T385-p QSKsPsRtLsPGRRA
S387-p KsPsRtLsPGRRAKs
S394-p sPGRRAKsRsLERKR
S396-p GRRAKsRsLERKRRE
S410-p EPERRRLssPRTRPR
S411-p PERRRLssPRTRPRD
T414 RRLssPRTRPRDDIL
S427-p ILGRCERsKDAsPIN
S431-p CERsKDAsPINRWsP
S437-p AsPINRWsPtRRRsR
T439-p PINRWsPtRRRsRsP
S443-p WsPtRRRsRsPIRRR
S445-p PtRRRsRsPIRRRSR
S458-p SRSPLRRsRsPRRRS
S460-p SPLRRsRsPRRRSRS
S507 KEDKFKGSLSEGMKV
S509 DKFKGSLSEGMKVEQ
S518-p GMKVEQEsssDDNLE
S519-p MKVEQEsssDDNLED
S520-p KVEQEsssDDNLEDF
Y552 RQAIVQKYKYLAEDS
Y554 AIVQKYKYLAEDSNI
S562 LAEDSNISVPSEPSs
S565 DSNISVPSEPSsPQS
S568 ISVPSEPSsPQSSTR
S569-p SVPSEPSsPQSSTRs
T574 PSsPQSSTRsRsPsP
S576-p sPQSSTRsRsPsPDD
S578-p QSSTRsRsPsPDDIL
S580-p STRsRsPsPDDILER
S622 TVEQNNGSSQKKILA
S623 VEQNNGSSQKKILAP
S636 APDMFTESDDMFAAY
Y643 SDDMFAAYFDSARLR
K656 LRAAGIGKDFKENPN
Y674 NWTDAEGYYRVNIGE
K717 ANQEVAVKIIRNNEL
K737-ub LKELEFLkKLNDADP
S794-p LHIKAVRsySQQLFL
Y795-p HIKAVRsySQQLFLA
S837-p LKLCDFGsAsHVADN
S839-p LCDFGsAsHVADNDI
T847-p HVADNDItPyLVsRF
Y849-p ADNDItPyLVsRFYR
S852-p DItPyLVsRFYRAPE
Y855 PyLVsRFYRAPEIII
K905 LKLAMDLKGKMPNKM
K907 LAMDLKGKMPNKMIR
  rat

 
S8 MAATEPPSLREQPEM
S21-p EMDDADNsEKsVNEE
S24-p DADNsEKsVNEENGE
S33-p NEENGEVsEDQSQNK
S37 GEVsEDQSQNKHSRH
S42 DQSQNKHSRHKKKKH
S88-p RKEVLDAsDKEGLsP
S94-p AsDKEGLsPAKRTKL
K112 ALLEDLEKQRALIKA
S132 LMEGKVQSGMGLILQ
Y141 MGLILQGYESGsEEE
S143 LILQGYESGsEEEGE
S145-p LQGYESGsEEEGEIH
T163 RNGNRSSTRSSSTRG
S165 GNRSSTRSSSTRGKL
S166 NRSSTRSSSTRGKLE
S167 RSSTRSSSTRGKLEI
T168 SSTRSSSTRGKLEIT
R169 STRSSSTRGKLEITD
K178 KLEITDNKNSAKKRS
K183 DNKNSAKKRSKSRSK
S185 KNSAKKRSKSRSKER
S197 KERTRHRSDKRKSKG
K201 RHRSDKRKSKGAVEM
S202 HRSDKRKSKGAVEMM
K203 RSDKRKSKGAVEMMR
S233 SPSKRSKSQDQARKs
S240-p SQDQARKsKsPTLRR
S242-p DQARKsKsPTLRRRS
T244 ARKsKsPTLRRRSQE
S249 sPTLRRRSQEKVGKA
S258 EKVGKARSPADEKIK
A260 VGKARSPADEKIKSE
S278-p KIKDRKKsPIVNERS
- gap
S285 sPIVNERSRDRSKKs
S292-p SRDRSKKsKsPVDLR
S294-p DRSKKsKsPVDLRDK
S328-p KEKKPIKsPSKDASS
S330 KKPIKsPSKDASSGK
S335 sPSKDASSGKENRSP
S349 PSRRPGRSPKRRSLS
S354 GRSPKRRSLSPKQRD
S356 SPKRRSLSPKQRDKS
K358 KRRSLSPKQRDKSRR
S366-p QRDKSRRsRsPLLND
S368-p DKSRRsRsPLLNDRR
S381 RRSKQSKSPSRTLsP
S383 SKQSKSPSRTLsPGR
T385 QSKSPSRTLsPGRRA
S387-p KSPSRTLsPGRRAKS
S394 sPGRRAKSRSLERKR
S396 GRRAKSRSLERKRRE
S410-p EPERRRLssPRTRPR
S411-p PERRRLssPRTRPRD
T414 RRLssPRTRPRDDIL
S427 ILGRCERSKDAsPIN
S431-p CERSKDAsPINRWsP
S437-p AsPINRWsPSRRRSR
S439 PINRWsPSRRRSRSP
S443 WsPSRRRSRSPIRRR
S445 PSRRRSRSPIRRRSR
S458 SRSPLRRSRSPRRRS
S460 SPLRRSRSPRRRSRS
S507 KEDKFKGSLSEGMKV
S509 DKFKGSLSEGMKVEQ
S518 GMKVEQESSSDDNLE
S519 MKVEQESSSDDNLED
S520 KVEQESSSDDNLEDF
Y552-p RQAIVQKyKyLAEDS
Y554-p AIVQKyKyLAEDSNI
S562 LAEDSNISVPSEPSS
S565 DSNISVPSEPSSPQS
S568 ISVPSEPSSPQSStR
S569 SVPSEPSSPQSStRs
T574-p PSSPQSStRsRsPsP
S576-p SPQSStRsRsPsPDD
S578-p QSStRsRsPsPDDIL
S580-p StRsRsPsPDDILER
S622 TVEQNNGSsQKKLLA
S623-p VEQNNGSsQKKLLAP
S636 APDMFTESDDMFAAY
Y643 SDDMFAAYFDSARLR
K656 LRAAGIGKDFKENPN
Y674 NWTDAEGYYRVNIGE
K717 ANQEVAVKIIRNNEL
K737 LKELEFLKKLNDADP
S794 LHIKAVRSYSQQLFL
Y795 HIKAVRSYSQQLFLA
S837 LKLCDFGSAsHVADN
S839-p LCDFGSAsHVADNDI
T847 HVADNDITPyLVsRF
Y849-p ADNDITPyLVsRFYR
S852-p DITPyLVsRFYRAPE
Y855 PyLVsRFYRAPEIII
K905 LKLAMDLKGKMPNKM
K907 LAMDLKGKMPNKMIR
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