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PRP4 (human)

Overview PRP4 a kinase of the DYRK family associated with the spliceosome and the kinetochore. It binds pre-mRNA splicing factors SFRS8, PRP6 and pinin. It is an essential kinase that associates with both the U5 snRNP and the N-CoR deacetylase complexes. It may help coordinate pre-mRNA splicing with chromatin remodeling and transcriptional regulation. PRP4 has recently been shown to be a kinetochore component that is necessary to establish a functional spindle assembly checkpoint, and is necessary for recruitment or maintenance of the checkpoint proteins RPS27, MAD1, and MAD2 at the kinetochores. Note: This description may include information from UniProtKB. Protein type: EC 2.7.11.1; Kinase, protein; RNA splicing; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); CMGC group; DYRK family; PRP4 subfamily Cellular Component: nucleolus; chromosome; nucleus Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; protein amino acid phosphorylation Reference #:  Q13523 (UniProtKB) Alt. Names/Synonyms: dJ1013A10.1; dJ1013A10.1 (PRP4 protein kinase homolog); KIAA0536; PR4H; protein-serine/threonine kinase; PRP4; PRP4 kinase; PRP4 pre-mRNA processing factor 4 homolog B (yeast); PRP4 pre-mRNA-processing factor 4 homolog; PRP4B; PRP4H; PRP4K; PRPF4B; Serine/threonine-protein kinase PRP4 homolog; serine/threonine-protein kinase PRP4K Gene Symbols: PRPF4B Molecular weight: 116,987 Da Basal Isoelectric point: 10.26  Predict pI for various phosphorylation states Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below PRP4

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	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
0	1		S8	MAAAETQSLREQPEM
0	24		S20-p	PEMEDANsEKsINEE
0	22		S23-p	EDANsEKsINEENGE
0	24		S32-p	NEENGEVsEDQsQNK
0	11		S36-p	GEVsEDQsQNKHsRH
0	1		S41-p	DQsQNKHsRHKKKKH
0	24		S87-p	RKEIIDAsDKEGMsP
0	26		S93-p	AsDKEGMsPAKRTKL
0	1		K111	ALLEDLEKQRALIKA
0	10		Y140-p	MGLILQGyEsGsEEE
0	27		S142-p	LILQGyEsGsEEEGE
0	34		S144-p	LQGyEsGsEEEGEIH
0	4		T162-p	RNGNRSStRsssTkG
0	23		S164-p	GNRSStRsssTkGKL
0	17		S165-p	NRSStRsssTkGKLE
0	20		S166-p	RSStRsssTkGKLEL
0	3		T167	SStRsssTkGKLELV
0	1		K168-a	StRsssTkGKLELVD
0	1		K177-a	KLELVDNkITTKkRs
0	1		K182-u	DNkITTKkRsKSRSK
0	2		S184-p	kITTKkRsKSRSKER
0	6		S196-p	KERTRHRsDKKkskG
0	1		K200-a	RHRsDKKkskGGIEI
0	1		S201-p	HRsDKKkskGGIEIV
0	1		K202-a	RsDKKkskGGIEIVK
0	2		S232-p	SPSKRSKsQDQARKs
0	25		S239-p	sQDQARKsKsPtLRR
0	25		S241-p	DQARKsKsPtLRRRs
0	2		T243-p	ARKsKsPtLRRRsQE
0	13		S248-p	sPtLRRRsQEKIGKA
0	11		S257-p	EKIGKARsPtDDKVK
0	4		T259-p	IGKARsPtDDKVKIE
0	72		S277-p	KSKDRKKsPIINEsR
0	3		S283-p	KsPIINEsRsRDRGK
0	1		S285-p	PIINEsRsRDRGKKs
0	21		S292-p	sRDRGKKsRsPVDLR
0	23		S294-p	DRGKKsRsPVDLRGK
0	15		S328-p	KEKKPIKsPsKDASS
0	5		S330-p	KKPIKsPsKDASSGK
0	1		S349-p	PSRRPGRsPKRRsLs
0	10		S354-p	GRsPKRRsLsPkPRD
0	10		S356-p	sPKRRsLsPkPRDKS
0	1		K358-a	KRRsLsPkPRDKSRR
0	19		S366-p	PRDKSRRsRsPLLND
0	18		S368-p	DKSRRsRsPLLNDRR
0	4		S381-p	RRSKQSKsPsRtLsP
0	4		S383-p	SKQSKsPsRtLsPGR
0	5		T385-p	QSKsPsRtLsPGRRA
0	14		S387-p	KsPsRtLsPGRRAKs
0	1		S394-p	sPGRRAKsRsLERKR
0	1		S396-p	GRRAKsRsLERKRRE
0	2		S410-p	EPERRRLssPRTRPR
0	3		S411-p	PERRRLssPRTRPRD
0	10		S427-p	ILSRRERsKDAsPIN
0	23		S431-p	RERsKDAsPINRWsP
0	17		S437-p	AsPINRWsPtRRRsR
0	4		T439-p	PINRWsPtRRRsRsP
0	3		S443-p	WsPtRRRsRsPIRRR
0	3		S445-p	PtRRRsRsPIRRRSR
0	3		S458-p	SRSPLRRsRsPRRRS
0	3		S460-p	SPLRRsRsPRRRSRS
0	1		S507-p	KEDKFKGsLsEGMKV
0	1		S509-p	DKFKGsLsEGMKVEQ
0	7		S518-p	GMKVEQEsssDDNLE
0	7		S519-p	MKVEQEsssDDNLED
0	6		S520-p	KVEQEsssDDNLEDF
0	1		Y552	RQAIVQKYKyLAEDS
0	2		Y554-p	AIVQKYKyLAEDSNM
0	2		S565-p	DSNMSVPsEPSsPQS
0	2		S569-p	SVPsEPSsPQSStRt
0	2		T574-p	PSsPQSStRtRsPsP
0	12		T576-p	sPQSStRtRsPsPDD
0	23		S578-p	QSStRtRsPsPDDIL
0	24		S580-p	StRtRsPsPDDILER
0	1		S636-p	APDMFTEsDDMFAAy
0	16		Y643-p	sDDMFAAyFDSARLR
0	2		K656-a	LRAAGIGkDFKENPN
0	1		Y674-p	NWTDAEGyYRVNIGE
0	1		K717-a	ANQEVAVkIIRNNEL
0	1		K737	LKELEFLKKLNDADP
0	2		S794-p	LHIKAVRsYSQQLFL
0	1		Y795	HIKAVRsYSQQLFLA
0	41		S837-p	LKLCDFGsAsHVADN
0	13		S839-p	LCDFGsAsHVADNDI
0	15		T847-p	HVADNDItPyLVsRF
1	4030		Y849-p	ADNDItPyLVsRFyR
0	384		S852-p	DItPyLVsRFyRAPE
0	3		Y855-p	PyLVsRFyRAPEIII
0	1		K905-a	LKLAMDLkGkMPNKM
0	1		K907-a	LAMDLkGkMPNKMIR

	mouse
S8-p	MAATEPPsLREQAEM
S21-p	EMDDADNsEKsVNEE
S24-p	DADNsEKsVNEENGE
S33-p	NEENGEVsEDQsQNK
S37-p	GEVsEDQsQNKHSRH
S42	DQsQNKHSRHKKKKH
S88-p	RKEVIEAsDKEGLsP
S94-p	AsDKEGLsPAKRTKL
K112-u	ALLEDLEkQRALIKA
Y141-p	MGLILQGyEsGsEEE
S143-p	LILQGyEsGsEEEGE
S145-p	LQGyEsGsEEEGEIH
T163	RNGNRSSTRssstRG
S165-p	GNRSSTRssstRGKL
S166-p	NRSSTRssstRGKLE
S167-p	RSSTRssstRGKLEI
T168-p	SSTRssstRGKLEIT
R169	STRssstRGKLEITD
K178	KLEITDNKNSAKKRS
K183	DNKNSAKKRSKSRSK
S185	KNSAKKRSKSRSKER
S197-p	KERTRHRsDKRKSKG
K201	RHRsDKRKSKGAGEM
S202	HRsDKRKSKGAGEML
K203	RsDKRKSKGAGEMLR
S233-p	SPSKRSKsQDQARKs
S240-p	sQDQARKsKsPPLRR
S242-p	DQARKsKsPPLRRRs
P244	ARKsKsPPLRRRsQE
S249-p	sPPLRRRsQEKVGKA
S258-p	EKVGKARsPAEEKMK
A260	VGKARsPAEEKMKSE
S278-p	KIKDRKKsPIVNERS
-	gap
S285	sPIVNERSRDRSKKs
S292-p	SRDRSKKsKsPVDLR
S294-p	DRSKKsKsPVDLRDK
S328-p	KEKKPIKsPsKDASS
S330-p	KKPIKsPsKDASSGK
S349	PSRRPGRSPKRRsLs
S354-p	GRSPKRRsLsPKLRD
S356-p	SPKRRsLsPKLRDKS
K358	KRRsLsPKLRDKSRR
S366-p	LRDKSRRsRsPLLND
S368-p	DKSRRsRsPLLNDRR
S381-p	RRSKQSKsPsRtLsP
S383-p	SKQSKsPsRtLsPGR
T385-p	QSKsPsRtLsPGRRA
S387-p	KsPsRtLsPGRRAKS
S394	sPGRRAKSRSLERKR
S396	GRRAKSRSLERKRRE
S410-p	EPERRRLssPRTRPR
S411-p	PERRRLssPRTRPRD
S427-p	ILGRCERsKDAsPIN
S431-p	CERsKDAsPINRWsP
S437-p	AsPINRWsPtRRRsR
T439-p	PINRWsPtRRRsRsP
S443-p	WsPtRRRsRsPIRRR
S445-p	PtRRRsRsPIRRRSR
S458-p	SRSPLRRsRsPRRRS
S460-p	SPLRRsRsPRRRSRS
S507	KEDKFKGSLSEGMKV
S509	DKFKGSLSEGMKVEQ
S518-p	GMKVEQEsssDDNLE
S519-p	MKVEQEsssDDNLED
S520-p	KVEQEsssDDNLEDF
Y552	RQAIVQKYKYLAEDS
Y554	AIVQKYKYLAEDSNI
S565	DSNISVPSEPSSPQS
S569	SVPSEPSSPQSSTRs
T574	PSSPQSSTRsRsPsP
S576-p	SPQSSTRsRsPsPDD
S578-p	QSSTRsRsPsPDDIL
S580-p	STRsRsPsPDDILER
S636	APDMFTESDDMFAAY
Y643	SDDMFAAYFDSARLR
K656	LRAAGIGKDFKENPN
Y674	NWTDAEGYYRVNIGE
K717	ANQEVAVKIIRNNEL
K737-u	LKELEFLkKLNDADP
S794-p	LHIKAVRsySQQLFL
Y795-p	HIKAVRsySQQLFLA
S837-p	LKLCDFGsAsHVADN
S839-p	LCDFGsAsHVADNDI
T847-p	HVADNDItPyLVsRF
Y849-p	ADNDItPyLVsRFYR
S852-p	DItPyLVsRFYRAPE
Y855	PyLVsRFYRAPEIII
K905	LKLAMDLKGKMPNKM
K907	LAMDLKGKMPNKMIR

	rat
S8	MAATEPPSLREQPEM
S21	EMDDADNSEKSVNEE
S24	DADNSEKSVNEENGE
S33	NEENGEVSEDQSQNK
S37	GEVSEDQSQNKHSRH
S42	DQSQNKHSRHKKKKH
S88	RKEVLDASDKEGLSP
S94	ASDKEGLSPAKRTKL
K112	ALLEDLEKQRALIKA
Y141	MGLILQGYESGSEEE
S143	LILQGYESGSEEEGE
S145	LQGYESGSEEEGEIH
T163	RNGNRSSTRSSSTRG
S165	GNRSSTRSSSTRGKL
S166	NRSSTRSSSTRGKLE
S167	RSSTRSSSTRGKLEI
T168	SSTRSSSTRGKLEIT
R169	STRSSSTRGKLEITD
K178	KLEITDNKNSAKKRS
K183	DNKNSAKKRSKSRSK
S185	KNSAKKRSKSRSKER
S197	KERTRHRSDKRKSKG
K201	RHRSDKRKSKGAVEM
S202	HRSDKRKSKGAVEMM
K203	RSDKRKSKGAVEMMR
S233	SPSKRSKSQDQARKS
S240	SQDQARKSKSPTLRR
S242	DQARKSKSPTLRRRS
T244	ARKSKSPTLRRRSQE
S249	SPTLRRRSQEKVGKA
S258	EKVGKARSPADEKIK
A260	VGKARSPADEKIKSE
S278	KIKDRKKSPIVNERS
-	gap
S285	SPIVNERSRDRSKKS
S292	SRDRSKKSKSPVDLR
S294	DRSKKSKSPVDLRDK
S328	KEKKPIKSPSKDASS
S330	KKPIKSPSKDASSGK
S349	PSRRPGRSPKRRSLS
S354	GRSPKRRSLSPKQRD
S356	SPKRRSLSPKQRDKS
K358	KRRSLSPKQRDKSRR
S366	QRDKSRRSRSPLLND
S368	DKSRRSRSPLLNDRR
S381	RRSKQSKSPSRTLSP
S383	SKQSKSPSRTLSPGR
T385	QSKSPSRTLSPGRRA
S387	KSPSRTLSPGRRAKS
S394	SPGRRAKSRSLERKR
S396	GRRAKSRSLERKRRE
S410	EPERRRLSSPRTRPR
S411	PERRRLSSPRTRPRD
S427	ILGRCERSKDASPIN
S431	CERSKDASPINRWSP
S437	ASPINRWSPSRRRSR
S439	PINRWSPSRRRSRSP
S443	WSPSRRRSRSPIRRR
S445	PSRRRSRSPIRRRSR
S458	SRSPLRRSRSPRRRS
S460	SPLRRSRSPRRRSRS
S507	KEDKFKGSLSEGMKV
S509	DKFKGSLSEGMKVEQ
S518	GMKVEQESSSDDNLE
S519	MKVEQESSSDDNLED
S520	KVEQESSSDDNLEDF
Y552-p	RQAIVQKyKyLAEDS
Y554-p	AIVQKyKyLAEDSNI
S565	DSNISVPSEPSSPQS
S569	SVPSEPSSPQSStRS
T574-p	PSSPQSStRSRSPSP
S576	SPQSStRSRSPSPDD
S578	QSStRSRSPSPDDIL
S580	StRSRSPSPDDILER
S636	APDMFTESDDMFAAY
Y643	SDDMFAAYFDSARLR
K656	LRAAGIGKDFKENPN
Y674	NWTDAEGYYRVNIGE
K717	ANQEVAVKIIRNNEL
K737	LKELEFLKKLNDADP
S794	LHIKAVRSYSQQLFL
Y795	HIKAVRSYSQQLFLA
S837	LKLCDFGSASHVADN
S839	LCDFGSASHVADNDI
T847	HVADNDITPyLVsRF
Y849-p	ADNDITPyLVsRFYR
S852-p	DITPyLVsRFYRAPE
Y855	PyLVsRFYRAPEIII
K905	LKLAMDLKGKMPNKM
K907	LAMDLKGKMPNKMIR

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