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Protein Page:
CDC7 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
CDC7 an evolutionarily conserved serine/threonine protein kinase of the CDC7 family. Predominantly localized in the nucleus, it plays a pivotal role in linking cell cycle regulation to genome duplication, being essential for the firing of DNA replication origins. Plays an essential role in initiating DNA replication at replication origins by phosphorylating the MCM2 and MCM4 subunits of the MCM2-7 helicase complex. The human protein requires phosphorylation by CDK1 at T376 and binding to activator of S-phase kinase (ASK)/DBF4B to be fully activated. The interaction between Cdc7 and DBFK subunits facilitates ATP binding and substrate recognition by the Cdc7 kinase. Although CDC7 expression levels appear to be constant throughout the cell cycle, the expression levels of DBF4B is cell cycle-regulated. Thus, the CDC7 activity is activated during S phase in eukaryotes. The Cdc7/ DBF4B complex regulates initiation of DNA replication by phosphorylating chromatin-bound mini-chromosome maintenance protein 2 (MCM2). Impaired Cdc7 kinase activity leads to S-phase arrest. Plays a role in the TGF-beta regulation of smooth muscle cell (SMC) differentiation. Interacts with Smad3 to facilitate Smad3 binding to SMC marker promoter, leading to the activation of SMC marker gene transcription. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Other group; CDC7 family
Chromosomal Location of Human Ortholog: 1p22
Cellular Component: nucleoplasm; microtubule cytoskeleton; intercellular bridge; cytoplasm; nucleolus; nucleus
Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; kinase activity; ATP binding; protein kinase activity
Biological Process: cell division; positive regulation of cell proliferation; mitotic cell cycle; DNA replication; protein amino acid phosphorylation; G1/S transition of mitotic cell cycle
Reference #:  O00311 (UniProtKB)
Alt. Names/Synonyms: CDC7; CDC7 (cell division cycle 7, S. cerevisiae, homolog)-like 1; CDC7-related kinase; CDC7L1; cell division cycle 7 homolog (S. cerevisiae); cell division cycle 7-like protein 1; Cell division cycle 7-related protein kinase; HsCdc7; Hsk1; huCdc7; MGC117361; MGC126237; MGC126238
Gene Symbols: CDC7
Molecular weight: 63,888 Da
Basal Isoelectric point: 8.96  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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CDC7

Protein Structure Not Found.

Substrate Sequence Logo
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Sites Implicated In
enzymatic activity, induced: T376‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S16-p MDEPMAFsPQRDRFQ
0 6 S27-p DRFQAEGsLKkNEQN
0 1 K30-ub QAEGsLKkNEQNFKL
0 1 S181-p VHRDVKPsNFLYNRR
0 1 K212-ub DTKIELLkFVQSEAQ
0 1 S277-p GKDGKEGsVGLSVQR
0 1 S297-p RNFNIHSsISHEsPA
0 3 S302-p HSsISHEsPAVKLMK
0 1 K331-ac KKKAISTkVMNSAVM
2 12 T376-p QVAPRAGtPGFRAPE
0 1 T386-p FRAPEVLtKCPNQTT
0 1 Y415-p LSGRYPFyKASDDLT
0 1 T472-p LRGMDSStPKLTSDI
0 1 S484-p SDIQGHAsHQPAIsE
0 1 S490-p AsHQPAIsEKTDHKA
0 2 S498-p EKTDHKAsCLVQtPP
0 6 T503-p KAsCLVQtPPGQYsG
0 2 S509-p QtPPGQYsGNsFKKG
0 1 S512-p PGQYsGNsFKKGDSN
0 1 S556-p LLDLNPAsRItAEEA
0 1 T559-p LNPAsRItAEEALLH
0 1 S573-p HPFFKDMsL______
  mouse

 
S8 MEEPMAFSSLRGSDR
S21 DRCPADDSLKKYEQS
K24 PADDSLKKYEQSVKL
S175 VHRDVKPSNFLYNRR
K206 DTKIELLKFVQSEAQ
S269 GKDGKERSVGLSVQR
S289 RNFNIHSSISHESPA
S294 HSSISHESPAEKLIK
K323 KKTAISTKAMNSVMR
T367-p QVAPRAGtPGFRAPE
T377 FRAPEVLTKCPDQTT
Y406 LSGRYPFYKASDDLT
T463 LRGLDSTTPRSASGP
S475 SGPPGNASYDPAASK
S481 ASYDPAASKNTDHKA
S489 KNTDHKASRVQAAQA
A493 HKASRVQAAQAQHsE
S499-p QAAQAQHsEDSLYKR
S502 QAQHsEDSLYKRDND
S546 LLDLNPASRITAEAA
T549 LNPASRITAEAALLH
C563 HAFFKDMCS______
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