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Protein Page:
eIF4A1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
eIF4A1 ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with PAIP1, EIF4E and UPF2. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. May interact with NOM1. Interacts with PDCD4; this interferes with the interaction between EIF4A and EIF4G. Interacts with RBM4. Interacts with human cytomegalovirus/HHV-5 protein UL69. Helicase activity and function in translation are inhibited by interaction with PDCD4. Belongs to the DEAD box helicase family. eIF4A subfamily. Note: This description may include information from UniProtKB.
Protein type: Translation initiation; EC 3.6.1.-; Helicase; RNA binding protein; Translation; EC 3.6.4.13
Cellular Component: membrane; eukaryotic translation initiation factor 4F complex; cytosol
Molecular Function: mRNA binding; protein binding; translation factor activity, nucleic acid binding; translation initiation factor activity; double-stranded RNA binding; ATP-dependent helicase activity; helicase activity; RNA cap binding; ATP binding
Biological Process: ATP catabolic process; poly(A) tail shortening; organ regeneration; cellular protein metabolic process; translation; viral reproduction; RNA metabolic process; cytokine and chemokine mediated signaling pathway; translational initiation; gene expression; mRNA metabolic process; mRNA catabolic process, deadenylation-dependent decay
Reference #:  P60842 (UniProtKB)
Alt. Names/Synonyms: ATP-dependent RNA helicase eIF4A-1; DDX2A; EIF-4A; eIF-4A-I; EIF4A; eIF4A-I; EIF4A1; Eukaryotic initiation factor 4A-I; eukaryotic initiation factor 4AI; eukaryotic translation initiation factor 4A, isoform 1; eukaryotic translation initiation factor 4A1; IF4A1
Gene Symbols: EIF4A1
Molecular weight: 46,154 Da
Basal Isoelectric point: 5.32  Predict pI for various phosphorylation states
CST Pathways:  Translation: eIF4E and p70S6K
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

eIF4A1

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S4-p ____MSAsQDSRSRD
0 85 Y48-p ESLLRGIyAyGFEkP
0 9 Y50-p LLRGIyAyGFEkPSA
0 116 K54-ub IyAyGFEkPSAIQQR
0 2 K68-ub RAILPCIkGyDVIAQ
0 1 K68-sc RAILPCIkGyDVIAQ
0 16 Y70-p ILPCIkGyDVIAQAQ
0 2 S78-p DVIAQAQsGtGKTAT
0 2 T80-p IAQAQsGtGKTATFA
0 1 T83 AQsGtGKTATFAISI
0 1 T101 IELDLKATQALVLAP
0 2 K118-ac ELAQQIQkVVMALGD
0 9 K118-ub ELAQQIQkVVMALGD
0 1 Y126-p VVMALGDyMGASCHA
0 1 K146-ac NVRAEVQkLQMEAPH
0 86 K146-ub NVRAEVQkLQMEAPH
0 4 T158-p APHIIVGtPGRVFDM
0 3 K174-ac NRRYLSPkYIkMFVL
0 39 K174-ub NRRYLSPkYIkMFVL
0 15 K177-ub YLSPkYIkMFVLDEA
0 2 K193-ac EMLSRGFkDQIyDIF
0 46 K193-ub EMLSRGFkDQIyDIF
0 1 K193-sc EMLSRGFkDQIyDIF
0 40 Y197-p RGFkDQIyDIFQkLN
0 2 K202-ub QIyDIFQkLNSNTQV
0 83 K225-ub SDVLEVTkkFMRDPI
0 1 K225-sc SDVLEVTkkFMRDPI
0 29 K226-ub DVLEVTkkFMRDPIR
0 5 K237-ub DPIRILVkkEELTLE
0 5 K238-ac PIRILVkkEELTLEG
0 13 K238-ub PIRILVkkEELTLEG
0 28 K284-ub IFINTRRkVDWLTEk
0 24 K291-ub kVDWLTEkMHARDFT
0 5 K309-ac MHGDMDQkERDVIMR
0 93 K309-ub MHGDMDQkERDVIMR
0 86 K369-ub RGGRFGRkGVAINMV
0 1 K381-ac NMVTEEDkRTLRDIE
0 61 K381-ub NMVTEEDkRTLRDIE
  mouse

 
S4 ____MSASQDSRSRD
Y48-p ESLLRGIyAyGFEkP
Y50-p LLRGIyAyGFEkPSA
K54-ub IyAyGFEkPSAIQQR
K68-ub RAILPCIkGyDVIAQ
K68 RAILPCIKGyDVIAQ
Y70-p ILPCIkGyDVIAQAQ
S78 DVIAQAQSGTGKTAT
T80 IAQAQSGTGKTATFA
T83 AQSGTGKTATFAISI
T101-p IELDLKAtQALVLAP
K118 ELAQQIQKVVMALGD
K118-ub ELAQQIQkVVMALGD
Y126-p VVMALGDyMGASCHA
K146 NVRAEVQKLQMEAPH
K146-ub NVRAEVQkLQMEAPH
T158 APHIIVGTPGRVFDM
K174-ac NRRYLSPkYIKMFVL
K174-ub NRRYLSPkYIKMFVL
K177 YLSPkYIKMFVLDEA
K193-ac EMLSRGFkDQIyDIF
K193-ub EMLSRGFkDQIyDIF
K193-sc EMLSRGFkDQIyDIF
Y197-p RGFkDQIyDIFQkLN
K202-ub QIyDIFQkLNSNTQV
K225-ub SDVLEVTkkFMRDPI
K225 SDVLEVTKkFMRDPI
K226-ub DVLEVTkkFMRDPIR
K237 DPIRILVKkEELTLE
K238 PIRILVKKEELTLEG
K238-ub PIRILVKkEELTLEG
K284-ub IFINTRRkVDWLTEk
K291-ub kVDWLTEkMHARDFT
K309-ac MHGDMDQkERDVIMR
K309-ub MHGDMDQkERDVIMR
K369-ub RGGRFGRkGVAINMV
K381 NMVTEEDKRTLRDIE
K381-ub NMVTEEDkRTLRDIE
  rat

 
S4 ____MSASQDSRSRD
Y48-p ESLLRGIyAYGFEkP
Y50 LLRGIyAYGFEkPSA
K54-ub IyAYGFEkPSAIQQR
K68 RAILPCIKGYDVIAQ
K68 RAILPCIKGYDVIAQ
Y70 ILPCIKGYDVIAQAQ
S78-p DVIAQAQsGtGKtAT
T80-p IAQAQsGtGKtATFA
T83-p AQsGtGKtATFAISI
T101 IELDLKATQALVLAP
K118 ELAQQIQKVVMALGD
K118 ELAQQIQKVVMALGD
Y126 VVMALGDYMGASCHA
K146 NVRAEVQKLQMEAPH
K146-ub NVRAEVQkLQMEAPH
T158 APHIIVGTPGRVFDM
K174 NRRYLSPKYIKMFVL
K174 NRRYLSPKYIKMFVL
K177 YLSPKYIKMFVLDEA
K193 EMLSRGFKDQIyDIF
K193 EMLSRGFKDQIyDIF
K193 EMLSRGFKDQIyDIF
Y197-p RGFKDQIyDIFQKLN
K202 QIyDIFQKLNSNTQV
K225 SDVLEVTKKFMRDPI
K225 SDVLEVTKKFMRDPI
K226 DVLEVTKKFMRDPIR
K237 DPIRILVKKEELTLE
K238 PIRILVKKEELTLEG
K238 PIRILVKKEELTLEG
K284 IFINTRRKVDWLTEK
K291 KVDWLTEKMHARDFT
K309 MHGDMDQKERDVIMR
K309 MHGDMDQKERDVIMR
K369 RGGRFGRKGVAINMV
K381 NMVTEEDKRTLRDIE
K381 NMVTEEDKRTLRDIE
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