Home

ULK1 (human)

Overview ULK1 Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. Interacts with GABARAP and GABARAPL2. Interacts (via C- terminus) with ATG13/KIAA0652. Part of a complex consisting of ATG13/KIAA0652, ULK1 and RB1CC1. Associates with the mammalian target of rapamycin complex 1 (mTORC1) through an interaction with RPTOR; the association depends on nutrient conditions and is reduced during starvation. Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. Note: This description may include information from UniProtKB. Protein type: Kinase, protein; Protein kinase, Other; Autophagy; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; EC 2.7.1.37; Other group; ULK family Cellular Component: neuron projection; cytoplasmic vesicle membrane; cell soma; pre-autophagosomal structure membrane; cytoplasm; autophagic vacuole; cytosol Molecular Function: protein serine/threonine kinase activity; protein binding; protein complex binding; protein kinase binding; ATP binding; Rab GTPase binding Biological Process: axon extension; protein amino acid autophosphorylation; neurite regeneration; negative regulation of collateral sprouting; cerebellar granule cell differentiation; protein amino acid phosphorylation; radial glia guided migration of granule cell; response to starvation; protein localization; cellular response to nutrient levels; receptor internalization; Ras protein signal transduction; regulation of autophagy; regulation of nerve growth factor receptor signaling pathway; positive regulation of macroautophagy; positive regulation of autophagy; neurite development; autophagic vacuole formation Reference #:  O75385 (UniProtKB) Alt. Names/Synonyms: ATG1; ATG1 autophagy related 1 homolog; FLJ38455; FLJ46475; KIAA0722; Serine/threonine-protein kinase ULK1; ULK1; Unc-51-like kinase 1; unc-51-like kinase 1 (C. elegans); UNC51; Unc51.1 Gene Symbols: ULK1 Molecular weight: 112,631 Da Basal Isoelectric point: 9.03  Predict pI for various phosphorylation states CST Pathways:  Autophagy Signaling Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below ULK1

STRING  |  Scansite  |  KinBase  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  ENZYME 2.7.1.37 2.7.11.1  |  DISEASE  |  Source  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene

	Sites Implicated In
autophagy, altered: S638‑p, S758‑p molecular association, regulation: S758‑p phosphorylation: S638‑p

	Modification Sites and Domains

	Modification Sites in Parent Protein, Orthologs, and Isoforms

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
0	1		S87	DFQEMANSVYLVMEY
0	1		S111-p	LHAMRTLsEDTIRLF
0	1		S147	KPQNILLSNPAGRRA
0	1		S158-p	GRRANPNsIRVKIAD
1	0		T180	QSNMMAATLCGSPMY
0	1		S195	MAPEVIMSQHYDGKA
0	1		S224	GKAPFQASSPQDLRL
0	1		S281	HHPFLDASPSVRKSP
0	1		P282	HPFLDASPSVRKSPP
0	1		S341	TAGFLHSSRDSGGSK
0	1		S405-p	HGRTPSPsPPCsSSP
0	1		S409-p	PSPsPPCsSSPSPsG
0	2		S415-p	CsSSPSPsGRAGPFS
0	1		S429-p	SSSRCGAsVPIPVPT
0	35		S450-p	RIERNLQsPTQFQtP
0	17		T456-p	QsPTQFQtPRssAIR
0	2		S459-p	TQFQtPRssAIRRsG
0	2		S460-p	QFQtPRssAIRRsGs
0	7		S465-p	RssAIRRsGstsPLG
3	13		S467-p	sAIRRsGstsPLGFA
0	4		T468-p	AIRRsGstsPLGFAR
0	22		S469-p	IRRsGstsPLGFARA
0	5		S477-p	PLGFARAsPsPPAHA
0	6		S479-p	GFARAsPsPPAHAEH
0	1		A482	RAsPsPPAHAEHGGV
0	2		S495-p	GVLARKMsLGGGRPY
0	2		S522	PGWSGTPSPQGAEMR
0	2		S544-p	GSSAPEHsPRTSGLG
3	28		S556-p	GLGCRLHsAPNLsDL
0	1		S561-p	LHsAPNLsDLHVVRP
0	7		T575-p	PKLPKPPtDPLGAVF
0	4		S588-p	VFSPPQAsPPQPSHG
0	1		S598-p	QPSHGLQsCRNLRGS
0	4		N615	LPDFLQRNPLPPILG
0	13		S623-p	PLPPILGsPtKAVPS
0	4		T625-p	PPILGsPtKAVPSFD
0	5		T636-p	PSFDFPKtPssQNLL
1	49		S638-p	FDFPKtPssQNLLAL
0	9		S639-p	DFPKtPssQNLLALL
0	1		T654-p	ARQGVVMtPPRNRtL
1	12		T660-p	MtPPRNRtLPDLSEV
0	3		S694-p	GPFGRSFstSRLtDL
0	5		T695-p	PFGRSFstSRLtDLL
0	3		T699-p	SFstSRLtDLLLKAA
0	1		T709-p	LLKAAFGtQAPDPGs
0	1		P714	FGtQAPDPGsTESLQ
0	2		S716-p	tQAPDPGsTESLQEK
0	2		T717	QAPDPGsTESLQEKP
0	3		S719	PDPGsTESLQEKPME
0	2		S748-p	ARAGGTSsPSPVVFT
1	8		S758-p	PVVFTVGsPPsGStP
0	1		S761-p	FTVGsPPsGStPPQG
0	3		T764-p	GsPPsGStPPQGPRT
1	2		S775-p	GPRTRMFsAGPTGsA
0	2		P778	TRMFsAGPTGsASSS
0	1		S781-p	FsAGPTGsASSSARH
0	1		S866	EIAALKGSASEAAGG
0	1		S912	KVAELLSSGLQSAID
0	5		S949-p	LYKASVVsCQGLSLR
0	1		S1042	LCIERRLSALLTGIC
1	1		T1046	RRLSALLTGICA___

	mouse
S87-p	DFQEMANsVYLVMEY
S111	LHTMRTLSEDTVRLF
S147-p	KPQNILLsNPGGRRA
N158	GRRANPSNIRVKIAD
T180-p	QSNMMAAtLCGSPMY
S195-p	MAPEVIMsQHYDGKA
S224-p	GKAPFQAsSPQDLRL
S281-p	HHPFLDAstPIKKSP
T282-p	HPFLDAstPIKKSPP
S341-p	AAGFLQGsRDSGGSS
S405	HGRTPSPSPTCSSSP
S409	PSPSPTCSSSPSPSG
S415	CSSSPSPSGRPGPFS
S429	SSNRYGASVPIPVPT
S450-p	RIEQNLQsPTQQQTA
T456	QsPTQQQTARssAIR
S459-p	TQQQTARssAIRRsG
S460-p	QQQTARssAIRRsGs
S465-p	RssAIRRsGstsPLG
S467-p	sAIRRsGsttPLGFG
T468-p	AIRRsGstsPLGFGR
T469-p	IRRsGsttPLGFGRA
S477-p	PLGFGRAsPsPPsHT
S479-p	GFGRAsPsPPsHTDG
S482-p	RAsPsPPsHTDGAML
S494	AMLARKLSLGGGRPY
S521-p	PSWSRVPsPQGADVR
S543-p	GSSVPEHsPRTTGLG
S555-p	GLGCRLHsAPNLSDF
S560	LHsAPNLSDFHVVRP
T574-p	PKLPKPPtDPLGATF
S587	TFSPPQTSAPQPCPG
S597	QPCPGLQSCRPLRGS
S614-p	LPDFLQRsPLPPILG
S622-p	PLPPILGsPtKAGPS
T624-p	PPILGsPtKAGPSFD
T635-p	PSFDFPKtPssQNLL
S637-p	FDFPKtPssQNLLTL
S638-p	DFPKtPssQNLLTLL
T653	ARQGVVMTPPRNRtL
T659-p	MTPPRNRtLPDLSEA
S693-p	GPFGRSFsTSRITDL
T694	PFGRSFsTSRITDLL
T698	SFsTSRITDLLLKAA
T708	LLKAAFGTQASDsGs
S713-p	FGTQASDsGstDsLQ
S715-p	TQASDsGstDsLQEK
T716-p	QASDsGstDsLQEKP
S718-p	SDsGstDsLQEKPME
S747-p	ARGGGASsPAPVVFT
S757-p	PVVFTVGsPPSGAtP
S760	FTVGsPPSGAtPPQS
T763-p	GsPPSGAtPPQSTRT
S774-p	STRTRMFsVGsSSsL
S777-p	TRMFsVGsSSsLGST
S780-p	FsVGsSSsLGSTGSS
S867-p	EIAALKGsASEAAGG
S913-p	KVAELLSsGLQTAID
S950-p	LYKASVVsCQGLSLR
S1043-p	LCIERRLsALLsGVY
S1047-p	RRLsALLsGVYA___

	rat
S87	DFQEMANSVYLVMEY
S111	LHTMRTLSEDTVRLF
S147	KPQNILLSNPGGRRA
N158	GRRANPSNIRVKIAD
T180	QSNMMAATLCGSPMY
S195	MAPEVIMSQHYDGKA
S224	GKAPFQASSPQDLRL
S281	HHPFLDASTPIKKSP
T282	HPFLDASTPIKKSPP
S341	AAGFLQGSRDSGGSS
S405	HGRTPSPSPTCSSSP
S409	PSPSPTCSSSPSPSG
S415	CSSSPSPSGRPGPFS
S429	SSNRYGASVPIPVPT
S450-p	RIEQNLQsPTQQQTA
T456	QsPTQQQTARSSAIR
S459	TQQQTARSSAIRRSG
S460	QQQTARSSAIRRSGs
S465	RSSAIRRSGsTsPLG
S467-p	SAIRRSGsTsPLGFG
T468	AIRRSGsTsPLGFGR
S469-p	IRRSGsTsPLGFGRA
S477	PLGFGRASPSPPSHT
S479	GFGRASPSPPSHTDG
S482	RASPSPPSHTDGAML
S494	AMLARKLSLGGGRPY
S521-p	PSWSRVPsPQGADVR
S543	GSSVPEHSPRTTGLG
S555	GLGCRLHSAPNLSDF
S560	LHSAPNLSDFHVVRP
T574	PKLPKPPTDPLGATF
S587	TFSPPQTSAPQPCPG
S597	QPCPGLQSCRPLRGS
S614	LPDFLQRSPLPPILG
S622-p	PLPPILGsPTKAGPS
T624	PPILGsPTKAGPSFD
A635	PSFDFPKAPSSQSLL
S637	FDFPKAPSSQSLLTL
S638	DFPKAPSSQSLLTLL
T653	ARQGVVMTPPRNRTL
T659	MTPPRNRTLPDLSEA
S693	GPFGRSFSTSRLTDL
T694	PFGRSFSTSRLTDLL
T698	SFSTSRLTDLLLKAA
T708	LLKAAFGTQASDSGS
S713	FGTQASDSGSTDSLQ
S715	TQASDSGSTDSLQEK
T716	QASDSGSTDSLQEKP
S718	SDSGSTDSLQEKPME
S747	ARAGGASSPAPVVFT
S757	PVVFTVGSPPSGTTP
S760	FTVGSPPSGTTPPQS
T763	GSPPSGTTPPQSTRT
S774	STRTRMFSVGSSSSL
S777	TRMFSVGSSSSLGST
S780	FSVGSSSSLGSTGSS
S867	EIAALKGSASEAAGG
S913	KVAELLSSGLQTAID
S950	LYKASVVSCQGLSLR
S1043	LCIERRLSALLSGVY
S1047	RRLSALLSGVYA___

Home  |  Curator Login With enhanced literature mining using Linguamatics I2E Produced by 3rd Millennium  |  Design by Digizyme ©2003-2013 Cell Signaling Technology, Inc.