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Protein Page:
ULK1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
ULK1 Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. Interacts with GABARAP and GABARAPL2. Interacts (via C- terminus) with ATG13/KIAA0652. Part of a complex consisting of ATG13/KIAA0652, ULK1 and RB1CC1. Associates with the mammalian target of rapamycin complex 1 (mTORC1) through an interaction with RPTOR; the association depends on nutrient conditions and is reduced during starvation. Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. Note: This description may include information from UniProtKB.
Protein type: Autophagy; Protein kinase, Other; Kinase, protein; EC 2.7.1.37; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Other group; ULK family
Cellular Component: neuron projection; cytoplasmic vesicle membrane; cell soma; pre-autophagosomal structure membrane; cytoplasm; autophagic vacuole; cytosol
Molecular Function: protein serine/threonine kinase activity; protein binding; protein complex binding; protein kinase binding; ATP binding; Rab GTPase binding
Biological Process: axon extension; protein amino acid autophosphorylation; neurite regeneration; negative regulation of collateral sprouting; protein amino acid phosphorylation; cerebellar granule cell differentiation; radial glia guided migration of granule cell; response to starvation; protein localization; cellular response to nutrient levels; receptor internalization; Ras protein signal transduction; regulation of autophagy; regulation of nerve growth factor receptor signaling pathway; positive regulation of macroautophagy; neurite development; positive regulation of autophagy; autophagic vacuole formation
Reference #:  O75385 (UniProtKB)
Alt. Names/Synonyms: ATG1; ATG1 autophagy related 1 homolog; FLJ38455; FLJ46475; KIAA0722; Serine/threonine-protein kinase ULK1; ULK1; Unc-51-like kinase 1; unc-51-like kinase 1 (C. elegans); UNC51; Unc51.1
Gene Symbols: ULK1
Molecular weight: 112,631 Da
Basal Isoelectric point: 9.03  Predict pI for various phosphorylation states
CST Pathways:  Autophagy Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

ULK1

Protein Structure Not Found.


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Sites Implicated In
autophagy, altered: S638‑p, S758‑p
molecular association, regulation: S758‑p
phosphorylation: S638‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S87 DFQEMANSVYLVMEY
0 1 S111-p LHAMRTLsEDTIRLF
0 1 S131-p GAMRLLHsKGIIHRD
0 1 S147 KPQNILLSNPAGRRA
0 1 S158-p GRRANPNsIRVKIAD
1 0 K162 NPNsIRVKIADFGFA
1 0 T180 QSNMMAATLCGSPMY
0 1 S195 MAPEVIMSQHYDGKA
0 1 S224 GKAPFQASsPQDLRL
0 1 S225-p KAPFQASsPQDLRLF
0 1 S281 HHPFLDASPSVRKSP
0 1 P282 HPFLDASPSVRKSPP
0 1 S341 TAGFLHSSRDSGGSK
0 1 S403-p ESHGRTPsPsPPCsS
0 1 S405-p HGRTPsPsPPCsSSP
0 1 S409-p PsPsPPCsSSPSPsG
0 3 S415-p CsSSPSPsGRAGPFS
0 1 S429-p SSSRCGAsVPIPVPT
0 39 S450-p RIERNLQsPTQFQtP
0 17 T456-p QsPTQFQtPRssAIR
0 2 S459-p TQFQtPRssAIRRsG
0 2 S460-p QFQtPRssAIRRsGs
0 8 S465-p RssAIRRsGstsPLG
3 16 S467-p sAIRRsGstsPLGFA
0 4 T468-p AIRRsGstsPLGFAR
0 26 S469-p IRRsGstsPLGFARA
0 9 S477-p PLGFARAsPsPPAHA
0 10 S479-p GFARAsPsPPAHAEH
0 1 A482 RAsPsPPAHAEHGGV
0 2 S495-p GVLARKMsLGGGRPY
0 2 S522 PGWSGTPSPQGAEMR
0 1 S533-p AEMRGGRsPRPGssA
0 1 S538-p GRsPRPGssAPEHsP
0 1 S539-p RsPRPGssAPEHsPR
0 2 S544-p GssAPEHsPRTSGLG
5 30 S556-p GLGCRLHsAPNLsDL
0 1 S561-p LHsAPNLsDLHVVRP
0 7 T575-p PKLPKPPtDPLGAVF
0 1 S583-p DPLGAVFsPPQAsPP
0 5 S588-p VFsPPQAsPPQPsHG
0 1 S593-p QAsPPQPsHGLQsCR
0 1 S598-p QPsHGLQsCRNLRGs
0 2 S605-p sCRNLRGsPKLPDFL
1 0 K607 RNLRGsPKLPDFLQR
0 4 N615 LPDFLQRNPLPPILG
0 15 S623-p PLPPILGsPtKAVPS
0 5 T625-p PPILGsPtKAVPSFD
0 6 T636-p PSFDFPKtPssQNLL
1 53 S638-p FDFPKtPssQNLLAL
0 10 S639-p DFPKtPssQNLLALL
0 1 T654-p ARQGVVMtPPRNRtL
1 12 T660-p MtPPRNRtLPDLSEV
0 3 S694-p GPFGRSFstSRLtDL
0 5 T695-p PFGRSFstSRLtDLL
0 3 T699-p SFstSRLtDLLLKAA
0 1 T709-p LLKAAFGtQAPDPGs
0 1 P714 FGtQAPDPGsTESLQ
0 2 S716-p tQAPDPGsTESLQEK
0 2 T717 QAPDPGsTESLQEKP
0 3 S719 PDPGsTESLQEKPME
0 1 S747 GARAGGTSsPSPVVF
0 2 S748-p ARAGGTSsPSPVVFT
2 10 S758-p PVVFTVGsPPsGStP
0 2 S761-p FTVGsPPsGStPPQG
0 3 T764-p GsPPsGStPPQGPRT
1 2 S775-p GPRTRMFsAGPTGsA
0 2 P778 TRMFsAGPTGsASSS
0 1 S781-p FsAGPTGsASSSARH
0 1 S866 EIAALKGSASEAAGG
0 1 S912 KVAELLSSGLQSAID
0 5 S949-p LYKASVVsCQGLSLR
0 1 S1042 LCIERRLSALLTGIC
1 1 T1046 RRLSALLTGICA___
4634 : Phospho-ULK1 (Ser467) Antibody
5869 : Phospho-ULK1 (Ser555) (D1H4) Rabbit mAb
12097 : Phospho-ULK1 (Ser638) Antibody
6888 : Phospho-ULK1 (Ser757) Antibody
  mouse

 
S87-p DFQEMANsVYLVMEY
S111 LHTMRTLSEDTVRLF
S131 GAMRLLHSKGIIHRD
S147-p KPQNILLsNPGGRRA
N158 GRRANPSNIRVkIAD
K162-ac NPSNIRVkIADFGFA
T180-p QSNMMAAtLCGSPMY
S195-p MAPEVIMsQHYDGKA
S224-p GKAPFQAsSPQDLRL
S225 KAPFQAsSPQDLRLF
S281-p HHPFLDAstPIKKSP
T282-p HPFLDAstPIKKSPP
S341-p AAGFLQGsRDSGGSS
S403 ESHGRTPSPSPTCSS
S405 HGRTPSPSPTCSSSP
S409 PSPSPTCSSSPSPSG
S415 CSSSPSPSGRPGPFS
S429 SSNRYGASVPIPVPT
S450-p RIEQNLQsPTQQQTA
T456 QsPTQQQTARssAIR
S459-p TQQQTARssAIRRsG
S460-p QQQTARssAIRRsGs
S465-p RssAIRRsGstsPLG
S467-p sAIRRsGsttPLGFG
T468-p AIRRsGstsPLGFGR
T469-p IRRsGsttPLGFGRA
S477-p PLGFGRAsPsPPsHT
S479-p GFGRAsPsPPsHTDG
S482-p RAsPsPPsHTDGAML
S494 AMLARKLSLGGGRPY
S521-p PSWSRVPsPQGADVR
S532 ADVRVGRSPRPGSSV
S537 GRSPRPGSSVPEHsP
S538 RSPRPGSSVPEHsPR
S543-p GSSVPEHsPRTTGLG
S555-p GLGCRLHsAPNLSDF
S560 LHsAPNLSDFHVVRP
T574-p PKLPKPPtDPLGATF
S582 DPLGATFSPPQTSAP
S587 TFSPPQTSAPQPCPG
C592 QTSAPQPCPGLQSCR
S597 QPCPGLQSCRPLRGS
S604 SCRPLRGSPkLPDFL
K606-ac RPLRGSPkLPDFLQR
S614-p LPDFLQRsPLPPILG
S622-p PLPPILGsPtKAGPS
T624-p PPILGsPtKAGPSFD
T635-p PSFDFPKtPssQNLL
S637-p FDFPKtPssQNLLTL
S638-p DFPKtPssQNLLTLL
T653 ARQGVVMTPPRNRtL
T659-p MTPPRNRtLPDLSEA
S693-p GPFGRSFsTSRITDL
T694 PFGRSFsTSRITDLL
T698 SFsTSRITDLLLKAA
T708 LLKAAFGTQASDsGs
S713-p FGTQASDsGstDsLQ
S715-p TQASDsGstDsLQEK
T716-p QASDsGstDsLQEKP
S718-p SDsGstDsLQEKPME
S746-p GARGGGAssPAPVVF
S747-p ARGGGAssPAPVVFT
S757-p PVVFTVGsPPsGAtP
S760-p FTVGsPPsGAtPPQS
T763-p GsPPsGAtPPQSTRT
S774-p STRTRMFsVGsSSsL
S777-p TRMFsVGsSSsLGST
S780-p FsVGsSSsLGSTGSS
S867-p EIAALKGsASEAAGG
S913-p KVAELLSsGLQTAID
S950-p LYKASVVsCQGLSLR
S1043-p LCIERRLsALLsGVY
S1047-p RRLsALLsGVYA___
4634 : Phospho-ULK1 (Ser467) Antibody
5869 : Phospho-ULK1 (Ser555) (D1H4) Rabbit mAb
12097 : Phospho-ULK1 (Ser638) Antibody
6888 : Phospho-ULK1 (Ser757) Antibody
  rat

 
S87 DFQEMANSVYLVMEY
S111 LHTMRTLSEDTVRLF
S131 GAMQLLHSKGIIHRD
S147 KPQNILLSNPGGRRA
N158 GRRANPSNIRVKIAD
K162 NPSNIRVKIADFGFA
T180 QSNMMAATLCGSPMY
S195 MAPEVIMSQHYDGKA
S224 GKAPFQASSPQDLRL
S225 KAPFQASSPQDLRLF
S281 HHPFLDASTPIKKSP
T282 HPFLDASTPIKKSPP
S341 AAGFLQGSRDSGGSS
S403 ESHGRTPSPSPTCSS
S405 HGRTPSPSPTCSSSP
S409 PSPSPTCSSSPSPSG
S415 CSSSPSPSGRPGPFS
S429 SSNRYGASVPIPVPT
S450-p RIEQNLQsPTQQQTA
T456 QsPTQQQTARSSAIR
S459 TQQQTARSSAIRRSG
S460 QQQTARSSAIRRSGs
S465 RSSAIRRSGsTsPLG
S467-p SAIRRSGsTsPLGFG
T468 AIRRSGsTsPLGFGR
S469-p IRRSGsTsPLGFGRA
S477 PLGFGRASPSPPSHT
S479 GFGRASPSPPSHTDG
S482 RASPSPPSHTDGAML
S494 AMLARKLSLGGGRPY
S521-p PSWSRVPsPQGADVR
S532 ADVRAGRSPRPGSSV
S537 GRSPRPGSSVPEHSP
S538 RSPRPGSSVPEHSPR
S543 GSSVPEHSPRTTGLG
S555 GLGCRLHSAPNLSDF
S560 LHSAPNLSDFHVVRP
T574 PKLPKPPTDPLGATF
S582 DPLGATFSPPQTSAP
S587 TFSPPQTSAPQPCPG
C592 QTSAPQPCPGLQSCR
S597 QPCPGLQSCRPLRGS
S604 SCRPLRGSPKLPDFL
K606 RPLRGSPKLPDFLQR
S614 LPDFLQRSPLPPILG
S622-p PLPPILGsPTKAGPS
T624 PPILGsPTKAGPSFD
A635 PSFDFPKAPSSQSLL
S637 FDFPKAPSSQSLLTL
S638 DFPKAPSSQSLLTLL
T653 ARQGVVMTPPRNRTL
T659 MTPPRNRTLPDLSEA
S693 GPFGRSFSTSRLTDL
T694 PFGRSFSTSRLTDLL
T698 SFSTSRLTDLLLKAA
T708 LLKAAFGTQASDSGS
S713 FGTQASDSGSTDSLQ
S715 TQASDSGSTDSLQEK
T716 QASDSGSTDSLQEKP
S718 SDSGSTDSLQEKPME
S746 GARAGGASSPAPVVF
S747 ARAGGASSPAPVVFT
S757 PVVFTVGSPPSGTTP
S760 FTVGSPPSGTTPPQS
T763 GSPPSGTTPPQSTRT
S774 STRTRMFSVGSSSSL
S777 TRMFSVGSSSSLGST
S780 FSVGSSSSLGSTGSS
S867 EIAALKGSASEAAGG
S913 KVAELLSSGLQTAID
S950 LYKASVVSCQGLSLR
S1043 LCIERRLSALLSGVY
S1047 RRLSALLSGVYA___
4634 : Phospho-ULK1 (Ser467) Antibody
5869 : Phospho-ULK1 (Ser555) (D1H4) Rabbit mAb
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