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Protein Page:
TTK (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
TTK a dual-specificity protein kinase orthologous to the yeast monopolar spindle (Mps1) kinase that is required for spindle pole duplication and the spindle checkpoint but not for centrosome duplication. Localized to centrosomes, kinetochores and nuclear pores. Its enzymatic activity is required for the recruitment of Mad1, Mad2, Bub1 and Bub3 to the kinetochore. Mps1 mRNA levels change little throughout the cell cycle, but its protein expression varies during the cell cycle. Overexpression arrests cells in metaphase by activating the checkpoint. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, Other; EC 2.7.12.1; Protein kinase, dual-specificity (non-receptor); Cancer Testis Antigen (CTA); Kinase, protein; Other group; TTK family
Cellular Component: spindle
Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; ATP binding
Biological Process: peptidyl-tyrosine phosphorylation; mitotic spindle organization and biogenesis; mitotic cell cycle spindle assembly checkpoint; positive regulation of cell proliferation; spindle organization and biogenesis
Reference #:  P33981 (UniProtKB)
Alt. Names/Synonyms: cancer/testis antigen 96; CT96; Dual specificity protein kinase TTK; ESK; FLJ38280; monopolar spindle 1-like 1; MPS1; MPS1L1; phosphotyrosine picked threonine kinase; Phosphotyrosine picked threonine-protein kinase; PYT; TTK; TTK protein kinase
Gene Symbols: TTK
Molecular weight: 97,072 Da
Basal Isoelectric point: 8.41  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

TTK

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
carcinogenesis, induced: S281‑p
cell cycle regulation: T288‑p, T676‑p, T686‑p
cytoskeletal reorganization: S281‑p, S436‑p
enzymatic activity, induced: S582‑p, T676‑p, T686‑p, S742‑p, Y811‑p
enzymatic activity, inhibited: T675‑p, T806‑p
intracellular localization: T12‑p, S15‑p, S821‑p
molecular association, regulation: T676‑p
protein stabilization: S281‑p, T288‑p, Y811‑p
ubiquitination: S281‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S3-p _____MEsEDLsGRE
0 3 S7-p _MEsEDLsGRELtID
2 1 T12-p DLsGRELtIDsIMNk
2 2 S15-p GRELtIDsIMNkVRD
0 1 K19-m1 tIDsIMNkVRDIKNK
2 15 T33-p KFKNEDLtDELsLNK
2 3 S37-p EDLtDELsLNKISAD
2 4 S80-p EKNSVPLsDALLNkL
0 4 K86-u LsDALLNkLIGRYSQ
0 3 S214-p TVLTAQEsFsGsLGH
0 1 S216-p LTAQEsFsGsLGHLQ
0 1 S218-p AQEsFsGsLGHLQNR
0 11 S258-p AEIGYRNsLRQTNKT
1 14 S281-p VPVNLLNsPDCDVKt
2 0 T288-p sPDCDVKtDDSVVPC
0 1 S317-p VVPGSKPsGNDsCEL
0 4 S321-p SKPsGNDsCELRNLK
0 1 T351-p KSSELIItDsITLKN
0 1 S353-p SELIItDsITLKNKt
2 1 T360-p sITLKNKtEssLLAK
1 0 S362-p TLKNKtEssLLAKLE
2 1 S363-p LKNKtEssLLAKLEE
0 2 T371-p LLAKLEEtkEyQEPE
0 1 K372-u LAKLEEtkEyQEPEV
0 83 Y374-p KLEEtkEyQEPEVPE
0 1 S382-p QEPEVPEsNQkQWQS
0 1 K385-m1 EVPEsNQkQWQSKRK
0 1 K385-u EVPEsNQkQWQSKRK
0 5 S393-p QWQSKRKsECINQNP
0 4 - gap
2 11 S436-p VFSVSKQsPPISTSK
1 10 T453-p DPKSICKtPsSNTLD
0 6 S455-p KSICKtPsSNTLDDy
0 61 Y462-p sSNTLDDyMSCFrtP
0 1 R467-m2 DDyMSCFrtPVVKND
1 3 T468-p DyMSCFrtPVVKNDF
0 1 K538-u IGSGGSSkVFQVLNE
0 1 K546-u VFQVLNEkKQIYAIK
1 0 T564-p LEEADNQtLDSYRNE
1 0 S582-p LNKLQQHsDKIIRLY
4 0 T675-p ANQMQPDttsVVKDS
7 2 T676-p NQMQPDttsVVKDSQ
2 0 S677-p QMQPDttsVVKDSQV
6 1 T686-p VKDSQVGtVNYMPPE
0 1 K696-u YMPPEAIkDMSSSRE
1 0 S742-p QQIINQIsKLHAIID
1 0 T795-p HPYVQIQtHPVNQMA
1 0 T805-p VNQMAKGttEEMkyV
1 0 T806-p NQMAKGttEEMkyVL
0 6 K810-u KGttEEMkyVLGQLV
1 7 Y811-p GttEEMkyVLGQLVG
7 15 S821-p GQLVGLNsPNsILkA
1 0 S824-p VGLNsPNsILkAAKT
0 1 K827-u NsPNsILkAAKTLyE
0 75 Y833-p LkAAKTLyEHySGGE
0 84 Y836-p AKTLyEHySGGESHN
1 0 S845-p GGESHNSsSsktFEK
1 0 S847-p ESHNSsSsktFEKKR
0 1 K848-u SHNSsSsktFEKKRG
1 0 T849-p HNSsSsktFEKKRGK
  mouse

► Hide Isoforms
 
A3 _____MEAEELIGSS
I7 _MEAEELIGSSVTID
T12 ELIGSSVTIDSIMSK
S15 GSSVTIDSIMSKMRD
K19 TIDSIMSKMRDIKNK
T32-p NKINEDCtDELSLSK
S36 EDCtDELSLSKICAD
N76 EKNSSPLNDDLLNKL
K82 LNDDLLNKLIGRYSQ
P210 TVLSAQEPFSSSLGN
S212 LSAQEPFSSSLGNVQ
S214 AQEPFSSSLGNVQNR
P254 AEVRHQNPFKQTHAA
S277-p VPVNLLNsPDFYVKT
T284 sPDFYVKTDSSAVTQ
R338 ILPGSRPRGSDSYEL
S342 SRPRGSDSYELRGLK
S371 EKSSELMSDLIALKS
- gap
T380 LIALKSKTDSSLTKL
S382 ALKSKTDSSLTKLEE
S383 LKSKTDSSLTKLEET
T390 SLTKLEETKPEIAER
K391 LTKLEETKPEIAERR
- gap
- gap
- gap
- gap
- gap
T428-p LRHVPDVtPKADKES
S435 tPKADKESPPISVPK
T452 DPKSACETPSSSSLD
S454 KSACETPSSSSLDDY
Y461 SSSSLDDYMKCFKTP
K466 DDYMKCFKTPVVKND
T467 DYMKCFKTPVVKNDF
K537 IGSGGSSKVFQVLNE
K545 VFQVLNEKKQINAIK
T563 LEDADSQTIESYRNE
S581 LNKLQQHSDKIIRLY
T674 ANQMQPDTTSIVKDS
T675 NQMQPDTTSIVKDSQ
S676 QMQPDTTSIVKDSQV
T685 VKDSQVGTVNYMAPE
R695 YMAPEAIRDMSSSRE
S741 QHIINQVSKLHAIIN
- gap
- gap
T805 SQMARGATDEMkYVL
K809-u RGATDEMkYVLGQLV
Y810 GATDEMkYVLGQLVG
S820-p GQLVGLNsPNSILKT
S823 VGLNsPNSILKTAKT
K826 NsPNSILKTAKTLYE
Y832 LKTAKTLYERYNCGE
Y835 AKTLYERYNCGEGQD
S844 CGEGQDSSSSKTFDK
S846 EGQDSSSSKTFDKKR
K847 GQDSSSSKTFDKKRE
T848 QDSSSSKTFDKKRER
  TTK iso2  
A3 _____MEAEELIGSS
I7 _MEAEELIGSSVTID
T12 ELIGSSVTIDSIMSK
S15 GSSVTIDSIMSKMRD
K19 TIDSIMSKMRDIKNK
T32 NKINEDCTDELSLSK
S36 EDCTDELSLSKICAD
N76 EKNSSPLNDDLLNKL
K82 LNDDLLNKLIGRYSQ
P210 TVLSAQEPFSSSLGN
S212 LSAQEPFSSSLGNVQ
S214 AQEPFSSSLGNVQNR
P254 AEVRHQNPFKQTHAA
S277 VPVNLLNSPDFYVKT
T284 SPDFYVKTDSSAVTQ
R312 ILPGSRPRGSDSYEL
S316 SRPRGSDSYELRGLK
S345 EKSSELMSDLIALKS
- gap
T354 LIALKSKTDSSLTKL
S356 ALKSKTDSSLTKLEE
S357 LKSKTDSSLTKLEET
T364 SLTKLEETKPEIAER
K365 LTKLEETKPEIAERR
- gap
- gap
- gap
- gap
- gap
T402 LRHVPDVTPKADKES
S409 TPKADKESPPISVPK
T426 DPKSACETPSSSSLD
S428 KSACETPSSSSLDDY
Y435 SSSSLDDYMKCFKTP
K440 DDYMKCFKTPVVKND
T441 DYMKCFKTPVVKNDF
K511 IGSGGSSKVFQVLNE
K519 VFQVLNEKKQINAIK
T537 LEDADSQTIESYRNE
S555 LNKLQQHSDKIIRLY
T648 ANQMQPDTTSIVKDS
T649 NQMQPDTTSIVKDSQ
S650 QMQPDTTSIVKDSQV
T659 VKDSQVGTVNYMAPE
R669 YMAPEAIRDMSSSRE
S715 QHIINQVSKLHAIIN
- gap
- gap
T779 SQMARGATDEMKYVL
K783 RGATDEMKYVLGQLV
Y784 GATDEMKYVLGQLVG
S794-p GQLVGLNsPNSILKT
S797 VGLNsPNSILKTAKV
K800 NsPNSILKTAKVSAC
- gap
- gap
- gap
- gap
- gap
- gap
  frog

 
D3 _____MDDEDISERK
S7 _MDDEDISERKLKIA
K12 DISERKLKIASILDR
S15 ERKLKIASILDRVKS
R19 KIASILDRVKSFKTK
T34 YGTDDNWTDELTFSK
T38 DNWTDELTFSKSSAD
I80 ENTGLPQIDPQLLNK
K87 IDPQLLNKLIDNYSQ
T215 SSHINQGTASFQNLA
S217 HINQGTASFQNLALG
Q219 NQGTASFQNLALGNP
P262 FEDIGRKPLLNMSAK
S283 VPVQPATSPDTRTRK
S291 PDTRTRKSDGSGSSS
A345 ILPHEEQANEDSLDM
S349 EEQANEDSLDMKTPS
S379 NEDLILGSTSTAVLN
- gap
- gap
- gap
S391 VLNPQENSKPSESAP
S396 ENSKPSESAPPLLPN
R407 LLPNINARLSDTTKP
- gap
- gap
- gap
- gap
- gap
- gap
S459 NPGSRRVSPPAPSLS
T476 DPVFVCGTPVNKSQE
- gap
Y485 VNKSQEDYMNCFRTP
R490 EDYMNCFRTPVVKTN
T491 DYMNCFRTPVVKTNL
K559 IGTGGSSKVFQVMDD
K567 VFQVMDDKKHLYAIK
T585 LEEADQQTIESYQNE
- gap
- gap
T697 NQIQPDVTSIVKDSQ
S698 QIQPDVTSIVKDSQV
T707 VKDSQVGTINYMPPE
R717 YMPPESIRDTTSYAE
- gap
- gap
T828 DQQVQKETTEEMKRI
T829 QQVQKETTEEMKRIL
K833 KETTEEMKRILGQLI
R834 ETTEEMKRILGQLIG
S844-p GQLIGLNsPNSISRA
S847 IGLNsPNSISRAAKN
R850 NsPNSISRAAKNLYD
Y856 SRAAKNLYDQFNSGR
- gap
- gap
- gap
- gap
T872 LDLSTLGTTVSQNTR
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