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TTK (human)

Overview TTK a dual-specificity protein kinase orthologous to the yeast monopolar spindle (Mps1) kinase that is required for spindle pole duplication and the spindle checkpoint but not for centrosome duplication. Localized to centrosomes, kinetochores and nuclear pores. Its enzymatic activity is required for the recruitment of Mad1, Mad2, Bub1 and Bub3 to the kinetochore. Mps1 mRNA levels change little throughout the cell cycle, but its protein expression varies during the cell cycle. Overexpression arrests cells in metaphase by activating the checkpoint. Note: This description may include information from UniProtKB. Protein type: Cancer Testis Antigen (CTA); Kinase, protein; Protein kinase, dual-specificity; EC 2.7.12.1; Protein kinase, Other; Other group; TTK family Cellular Component: spindle Molecular Function: protein serine/threonine kinase activity; identical protein binding; protein-tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; ATP binding Biological Process: mitotic spindle organization and biogenesis; positive regulation of cell proliferation; mitotic cell cycle spindle assembly checkpoint; spindle organization and biogenesis Reference #:  P33981 (UniProtKB) Alt. Names/Synonyms: cancer/testis antigen 96; CT96; Dual specificity protein kinase TTK; ESK; FLJ38280; monopolar spindle 1-like 1; MPS1; MPS1L1; phosphotyrosine picked threonine kinase; Phosphotyrosine picked threonine-protein kinase; PYT; TTK; TTK protein kinase Gene Symbols: TTK Molecular weight: 97,072 Da Basal Isoelectric point: 8.41  Predict pI for various phosphorylation states Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below TTK 2X9E - A=514-828 (human) 2ZMC - A=510-857 (human) 2ZMD - A=510-857 (human) 3CEK - A=519-808 (human) 3DBQ - A=515-857 (human) 3GFW - A=519-808 (human) 3H9F - A=519-808 (human) 3HMN - A=510-809 (human) 3HMO - A=510-809 (human) 3HMP - A=510-809 (human) 3VQU - A=516-820 (human) 4B94 - A/D/C/B=62-239 (human) 4H7X - A/B=55-210 (human) 4H7Y - A/D/C/B=55-210 (human) Substrate Sequence Logo

STRING  |  Scansite  |  KinBase  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB 2X9E 2ZMC 2ZMD 3CEK 3DBQ 3GFW 3H9F 3HMN 3HMO 3HMP 3VQU 4B94 4H7X 4H7Y  |  ENZYME  |  Phospho3D  |  DISEASE  |  Source  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene

	Sites Implicated In
carcinogenesis, induced: S281‑p cell cycle regulation: T288‑p, T676‑p, T686‑p cytoskeletal reorganization: S281‑p, S436‑p enzymatic activity, induced: S582‑p, T676‑p, T686‑p, S742‑p, Y811‑p enzymatic activity, inhibited: T675‑p, T806‑p intracellular localization: T12‑p, S15‑p, S821‑p molecular association, regulation: T676‑p protein stabilization: S281‑p, T288‑p, Y811‑p ubiquitination: S281‑p

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
0	1		S3-p	_____MEsEDLsGRE
0	2		S7-p	_MEsEDLsGRELtID
2	1		T12-p	DLsGRELtIDsIMNK
2	2		S15-p	GRELtIDsIMNKVRD
2	12		T33-p	KFKNEDLtDELsLNK
2	3		S37-p	EDLtDELsLNKISAD
2	4		S80-p	EKNSVPLsDALLNkL
0	4		K86-u	LsDALLNkLIGRYSQ
0	1		Y131-p	EPDDARDyFQMARAN
0	1		K200	QLLSEEEKKNLSAST
0	3		S214-p	TVLTAQEsFsGsLGH
0	1		S216-p	LTAQEsFsGsLGHLQ
0	1		S218-p	AQEsFsGsLGHLQNR
0	1		Y255-p	PQDAEIGyRNsLRQT
0	11		S258-p	AEIGyRNsLRQTNKT
1	12		S281-p	VPVNLLNsPDCDVKt
2	0		T288-p	sPDCDVKtDDSVVPC
0	1		S317-p	VVPGSKPsGNDsCEL
0	3		S321-p	SKPsGNDsCELRNLK
0	1		T351-p	KSSELIItDsITLKN
0	1		S353-p	SELIItDsITLKNKt
2	1		T360-p	sITLKNKtEssLLAK
1	0		S362-p	TLKNKtEssLLAKLE
2	1		S363-p	LKNKtEssLLAKLEE
0	2		T371-p	LLAKLEEtkEyQEPE
0	1		K372-u	LAKLEEtkEyQEPEV
0	83		Y374-p	KLEEtkEyQEPEVPE
0	1		S382-p	QEPEVPEsNQkQWQS
0	1		K385-m1	EVPEsNQkQWQSKRK
0	1		K385-u	EVPEsNQkQWQSKRK
0	5		S393-p	QWQSKRKsECINQNP
0	4		-	gap
2	10		S436-p	VFSVSKQsPPISTSK
1	10		T453-p	DPKSICKtPsSNtLD
0	5		S455-p	KSICKtPsSNtLDDy
0	1		T458-p	CKtPsSNtLDDyMSC
0	61		Y462-p	sSNtLDDyMSCFrtP
0	1		R467-m2	DDyMSCFrtPVVKND
1	3		T468-p	DyMSCFrtPVVKNDF
0	1		K538-u	IGSGGSSkVFQVLNE
0	1		K546-u	VFQVLNEkKQIYAIK
1	0		T564-p	LEEADNQtLDSYRNE
1	0		S582-p	LNKLQQHsDKIIRLY
0	1		S618-p	SWLKKKKsIDPWERK
4	0		T675-p	ANQMQPDttsVVKDS
7	2		T676-p	NQMQPDttsVVKDSQ
2	0		S677-p	QMQPDttsVVKDSQV
6	1		T686-p	VKDSQVGtVNYMPPE
0	1		K696-u	YMPPEAIkDMSSSRE
1	0		S742-p	QQIINQIsKLHAIID
1	0		T795-p	HPYVQIQtHPVNQMA
1	0		T805-p	VNQMAKGttEEMkyV
1	0		T806-p	NQMAKGttEEMkyVL
0	6		K810-u	KGttEEMkyVLGQLV
1	7		Y811-p	GttEEMkyVLGQLVG
7	14		S821-p	GQLVGLNsPNsILkA
1	0		S824-p	VGLNsPNsILkAAKT
0	1		K827-u	NsPNsILkAAKTLyE
0	75		Y833-p	LkAAKTLyEHysGGE
0	84		Y836-p	AKTLyEHysGGESHN
0	1		S837-p	KTLyEHysGGESHNS
1	0		S845-p	GGESHNSsSsktFEk
1	0		S847-p	ESHNSsSsktFEkkR
0	1		K848-u	SHNSsSsktFEkkRG
1	0		T849-p	HNSsSsktFEkkRGK
0	1		K852-a	sSsktFEkkRGKK__
0	1		K853-a	SsktFEkkRGKK___

	mouse ► Hide Isoforms
A3	_____MEAEELIGSS
I7	_MEAEELIGSSVTID
T12	ELIGSSVTIDSIMSK
S15	GSSVTIDSIMSKMRD
T32-p	NKINEDCtDELSLSK
S36	EDCtDELSLSKICAD
N76	EKNSSPLNDDLLNKL
K82	LNDDLLNKLIGRYSQ
Y127	EPDDARDYFQMAREN
K196-a	QLLPEEDkKSVSAST
P210	TVLSAQEPFSSSLGN
S212	LSAQEPFSSSLGNVQ
S214	AQEPFSSSLGNVQNR
H251	PQDAEVRHQNPFKQT
P254	AEVRHQNPFKQTHAA
S277-p	VPVNLLNsPDFYVKT
T284	sPDFYVKTDSSAVTQ
R338	ILPGSRPRGSDSYEL
S342	SRPRGSDSYELRGLK
S371	EKSSELMSDLIALKS
L373	SSELMSDLIALKSKT
T380	LIALKSKTDSSLTKL
S382	ALKSKTDSSLTKLEE
S383	LKSKTDSSLTKLEET
T390	SLTKLEETKPEIAER
K391	LTKLEETKPEIAERR
-	gap
R397	TKPEIAERRPMQWQS
M400	EIAERRPMQWQSTRK
M400	EIAERRPMQWQSTRK
P408	QWQSTRKPECVFQNP
T428-p	LRHVPDVtPKADKES
S435	tPKADKESPPISVPK
T452	DPKSACETPSSSSLD
S454	KSACETPSSSSLDDY
S457	CETPSSSSLDDYMKC
Y461	SSSSLDDYMKCFKTP
K466	DDYMKCFKTPVVKND
T467	DYMKCFKTPVVKNDF
K537	IGSGGSSKVFQVLNE
K545	VFQVLNEKKQINAIK
T563	LEDADSQTIESYRNE
S581	LNKLQQHSDKIIRLY
S617	SWLKKKKSINPWERK
T674	ANQMQPDTTSIVKDS
T675	NQMQPDTTSIVKDSQ
S676	QMQPDTTSIVKDSQV
T685	VKDSQVGTVNYMAPE
R695	YMAPEAIRDMSSSRE
S741	QHIINQVSKLHAIIN
P794	HPYVQIQPHPGSQMA
A804	GSQMARGATDEMkYV
T805	SQMARGATDEMkYVL
K809-u	RGATDEMkYVLGQLV
Y810	GATDEMkYVLGQLVG
S820-p	GQLVGLNsPNSILKT
S823	VGLNsPNSILKTAKT
K826	NsPNSILKTAKTLYE
Y832	LKTAKTLYERYNCGE
Y835	AKTLYERYNCGEGQD
N836	KTLYERYNCGEGQDS
S844	CGEGQDSSSSKTFDK
S846	EGQDSSSSKTFDKKR
K847	GQDSSSSKTFDKKRE
T848	QDSSSSKTFDKKRER
K851	SSSKTFDKKRERK__
K852	SSKTFDKKRERK___

	TTK iso2
A3	_____MEAEELIGSS
I7	_MEAEELIGSSVTID
T12	ELIGSSVTIDSIMSK
S15	GSSVTIDSIMSKMRD
T32	NKINEDCTDELSLSK
S36	EDCTDELSLSKICAD
N76	EKNSSPLNDDLLNKL
K82	LNDDLLNKLIGRYSQ
Y127	EPDDARDYFQMAREN
K196	QLLPEEDKKSVSAST
P210	TVLSAQEPFSSSLGN
S212	LSAQEPFSSSLGNVQ
S214	AQEPFSSSLGNVQNR
H251	PQDAEVRHQNPFKQT
P254	AEVRHQNPFKQTHAA
S277	VPVNLLNSPDFYVKT
T284	SPDFYVKTDSSAVTQ
R312	ILPGSRPRGSDSYEL
S316	SRPRGSDSYELRGLK
S345	EKSSELMSDLIALKS
L347	SSELMSDLIALKSKT
T354	LIALKSKTDSSLTKL
S356	ALKSKTDSSLTKLEE
S357	LKSKTDSSLTKLEET
T364	SLTKLEETKPEIAER
K365	LTKLEETKPEIAERR
-	gap
R371	TKPEIAERRPMQWQS
M374	EIAERRPMQWQSTRK
M374	EIAERRPMQWQSTRK
P382	QWQSTRKPECVFQNP
T402	LRHVPDVTPKADKES
S409	TPKADKESPPISVPK
T426	DPKSACETPSSSSLD
S428	KSACETPSSSSLDDY
S431	CETPSSSSLDDYMKC
Y435	SSSSLDDYMKCFKTP
K440	DDYMKCFKTPVVKND
T441	DYMKCFKTPVVKNDF
K511	IGSGGSSKVFQVLNE
K519	VFQVLNEKKQINAIK
T537	LEDADSQTIESYRNE
S555	LNKLQQHSDKIIRLY
S591	SWLKKKKSINPWERK
T648	ANQMQPDTTSIVKDS
T649	NQMQPDTTSIVKDSQ
S650	QMQPDTTSIVKDSQV
T659	VKDSQVGTVNYMAPE
R669	YMAPEAIRDMSSSRE
S715	QHIINQVSKLHAIIN
P768	HPYVQIQPHPGSQMA
A778	GSQMARGATDEMKYV
T779	SQMARGATDEMKYVL
K783	RGATDEMKYVLGQLV
Y784	GATDEMKYVLGQLVG
S794-p	GQLVGLNsPNSILKT
S797	VGLNsPNSILKTAKV
K800	NsPNSILKTAKVSAC
-	gap
-	gap
-	gap
-	gap
-	gap
-	gap
-	gap
-	gap
-	gap

	frog
D3	_____MDDEDISERK
S7	_MDDEDISERKLKIA
K12	DISERKLKIASILDR
S15	ERKLKIASILDRVKS
T34	YGTDDNWTDELTFSK
T38	DNWTDELTFSKSSAD
I80	ENTGLPQIDPQLLNK
K87	IDPQLLNKLIDNYSQ
Q132	DLDEAREQFQFARLN
K201	QLISDEDKENLAVSS
T215	SSHINQGTASFQNLA
S217	HINQGTASFQNLALG
Q219	NQGTASFQNLALGNP
G259	PEDFEDIGRKPLLNM
P262	FEDIGRKPLLNMSAK
S283	VPVQPATSPDTRTRK
S291	PDTRTRKSDGSGSSS
A345	ILPHEEQANEDSLDM
S349	EEQANEDSLDMKTPS
S379	NEDLILGSTSTAVLN
S381	DLILGSTSTAVLNPQ
Q388	STAVLNPQENSKPSE
N390	AVLNPQENSKPSESA
S391	VLNPQENSKPSESAP
S396	ENSKPSESAPPLLPN
A397	NSKPSESAPPLLPNI
P399	KPSESAPPLLPNINA
T412	NARLSDTTKPNNIEA
N415	LSDTTKPNNIEAEWK
N415	LSDTTKPNNIEAEWK
-	gap
-	gap
S459	NPGSRRVSPPAPSLS
T476	DPVFVCGTPVNKSQE
V478	VFVCGTPVNKSQEDY
S481	CGTPVNKSQEDYMNC
Y485	VNKSQEDYMNCFRTP
R490	EDYMNCFRTPVVKTN
T491	DYMNCFRTPVVKTNL
K559	IGTGGSSKVFQVMDD
K567	VFQVMDDKKHLYAIK
T585	LEEADQQTIESYQNE
C603	LNKLQQHCDKIIRLF
T639	TWLRKKKTINPWERK
V696	ANQIQPDVTSIVKDS
T697	NQIQPDVTSIVKDSQ
S698	QIQPDVTSIVKDSQV
T707	VKDSQVGTINYMPPE
R717	YMPPESIRDTTSYAE
A763	QHITNQIAKLHSILD
P816	HPYVQIQPHTQPDQQ
T828	DQQVQKETTEEMKRI
T829	QQVQKETTEEMKRIL
K833	KETTEEMKRILGQLI
R834	ETTEEMKRILGQLIG
S844-p	GQLIGLNsPNSISRA
S847	IGLNsPNSISRAAKN
R850	NsPNSISRAAKNLYD
Y856	SRAAKNLYDQFNSGR
F859	AKNLYDQFNSGRSLD
N860	KNLYDQFNSGRSLDL
S868	SGRSLDLSTLGTTVS
L870	RSLDLSTLGTTVSQN
G871	SLDLSTLGTTVSQNT
T872	LDLSTLGTTVSQNTR
S875	STLGTTVSQNTRTTK
Q876	TLGTTVSQNTRTTK_

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