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Protein Page:
TTK (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
TTK a dual-specificity protein kinase orthologous to the yeast monopolar spindle (Mps1) kinase that is required for spindle pole duplication and the spindle checkpoint but not for centrosome duplication. Localized to centrosomes, kinetochores and nuclear pores. Its enzymatic activity is required for the recruitment of Mad1, Mad2, Bub1 and Bub3 to the kinetochore. Mps1 mRNA levels change little throughout the cell cycle, but its protein expression varies during the cell cycle. Overexpression arrests cells in metaphase by activating the checkpoint. Note: This description may include information from UniProtKB.
Protein type: Kinase, protein; EC 2.7.12.1; Protein kinase, Other; Protein kinase, dual-specificity (non-receptor); Cancer Testis Antigen (CTA); Other group; TTK family
Cellular Component: membrane; spindle
Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; ATP binding
Biological Process: mitotic spindle organization and biogenesis; peptidyl-tyrosine phosphorylation; mitotic cell cycle spindle assembly checkpoint; positive regulation of cell proliferation; spindle organization and biogenesis; chromosome separation
Reference #:  P33981 (UniProtKB)
Alt. Names/Synonyms: cancer/testis antigen 96; CT96; Dual specificity protein kinase TTK; ESK; FLJ38280; monopolar spindle 1-like 1; MPS1; MPS1L1; phosphotyrosine picked threonine kinase; Phosphotyrosine picked threonine-protein kinase; PYT; TTK; TTK protein kinase
Gene Symbols: TTK
Molecular weight: 97,072 Da
Basal Isoelectric point: 8.41  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

TTK

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
carcinogenesis, induced: S281‑p
cell cycle regulation: T288‑p, T676‑p, T686‑p
cytoskeletal reorganization: S281‑p, S436‑p
enzymatic activity, induced: S582‑p, T676‑p, T686‑p, S742‑p, Y811‑p
enzymatic activity, inhibited: T675‑p, T806‑p
intracellular localization: T12‑p, S15‑p, S821‑p
molecular association, regulation: T676‑p
protein stabilization: S281‑p, T288‑p, Y811‑p
ubiquitination: S281‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S3-p _____MEsEDLsGRE
1 4 S7-p _MEsEDLsGRELtID
3 1 T12-p DLsGRELtIDsIMNk
3 2 S15-p GRELtIDsIMNkVRD
0 1 K19-m1 tIDsIMNkVRDIKNK
3 24 T33-p KFKNEDLtDELsLNK
3 5 S37-p EDLtDELsLNKISAD
2 5 S80-p EKNSVPLsDALLNkL
0 4 K86-ub LsDALLNkLIGRYSQ
0 1 Y131-p EPDDARDyFQMARAN
0 1 K200 QLLSEEEKKNLSAST
0 3 S214-p TVLTAQEsFsGsLGH
0 1 S216-p LTAQEsFsGsLGHLQ
0 1 S218-p AQEsFsGsLGHLQNR
0 1 Y255-p PQDAEIGyRNsLRQT
0 10 S258-p AEIGyRNsLRQTNKT
1 18 S281-p VPVNLLNsPDCDVKt
4 0 T288-p sPDCDVKtDDsVVPC
0 1 S291-p CDVKtDDsVVPCFMK
0 2 S317-p VVPGSKPsGNDsCEL
1 6 S321-p SKPsGNDsCELRNLK
0 1 T351-p KSSELIItDsITLKN
0 1 S353-p SELIItDsITLKNKt
3 2 T360-p sITLKNKtEssLLAK
1 0 S362-p TLKNKtEssLLAKLE
3 2 S363-p LKNKtEssLLAKLEE
1 2 T371-p LLAKLEEtkEyQEPE
0 1 K372-ub LAKLEEtkEyQEPEV
0 68 Y374-p KLEEtkEyQEPEVPE
0 3 S382-p QEPEVPEsNQkQWQS
0 1 K385-m1 EVPEsNQkQWQSKRK
0 1 K385-ub EVPEsNQkQWQSKRK
0 6 S393-p QWQSKRKsECINQNP
0 4 - gap
0 1 S433-p EQPVFSVsKQsPPIs
2 14 S436-p VFSVsKQsPPIsTSK
0 1 S440-p sKQsPPIsTSKWFDP
1 12 T453-p DPKSICKtPsSNtLD
0 8 S455-p KSICKtPsSNtLDDy
0 1 T458-p CKtPsSNtLDDyMsC
0 59 Y462-p sSNtLDDyMsCFrtP
0 1 S464-p NtLDDyMsCFrtPVV
0 1 R467-m2 DDyMsCFrtPVVKND
1 4 T468-p DyMsCFrtPVVKNDF
0 1 K538-ub IGSGGSSkVFQVLNE
0 1 K546-ub VFQVLNEkKQIYAIK
1 0 T564-p LEEADNQtLDSYRNE
1 0 S582-p LNKLQQHsDKIIRLY
0 1 S618-p SWLKKKKsIDPWERK
4 0 T675-p ANQMQPDttsVVKDS
8 2 T676-p NQMQPDttsVVKDSQ
2 0 S677-p QMQPDttsVVKDSQV
7 1 T686-p VKDSQVGtVNYMPPE
0 1 K696-ub YMPPEAIkDMSSSRE
1 0 S742-p QQIINQIsKLHAIID
1 0 T795-p HPYVQIQtHPVNQMA
1 0 T805-p VNQMAKGttEEMkyV
1 0 T806-p NQMAKGttEEMkyVL
0 6 K810-ub KGttEEMkyVLGQLV
1 18 Y811-p GttEEMkyVLGQLVG
7 19 S821-p GQLVGLNsPNsILkA
1 2 S824-p VGLNsPNsILkAAKT
0 1 K827-ub NsPNsILkAAKTLyE
0 62 Y833-p LkAAKTLyEHysGGE
0 66 Y836-p AKTLyEHysGGESHN
0 2 S837-p KTLyEHysGGESHNS
1 0 S845-p GGESHNSsSsktFEk
1 0 S847-p ESHNSsSsktFEkkR
0 1 K848-ub SHNSsSsktFEkkRG
1 0 T849-p HNSsSsktFEkkRGK
0 1 K852-ac sSsktFEkkRGKK__
0 1 K853-ac SsktFEkkRGKK___
  mouse

► Hide Isoforms
 
A3 _____MEAEELIGSS
I7 _MEAEELIGSSVTID
T12 ELIGSSVTIDSIMSK
S15 GSSVTIDSIMSKMRD
K19 TIDSIMSKMRDIKNK
T32-p NKINEDCtDELSLSK
S36 EDCtDELSLSKICAD
N76 EKNSSPLNDDLLNKL
K82 LNDDLLNKLIGRYSQ
Y127 EPDDARDYFQMAREN
K196-ac QLLPEEDkKSVSAST
P210 TVLSAQEPFSSSLGN
S212 LSAQEPFSSSLGNVQ
S214 AQEPFSSSLGNVQNR
H251 PQDAEVRHQNPFKQT
P254 AEVRHQNPFKQTHAA
S277-p VPVNLLNsPDFYVKT
T284 sPDFYVKTDSSAVTQ
S287 FYVKTDSSAVTQLTT
R338 ILPGSRPRGSDSYEL
S342 SRPRGSDSYELRGLK
S371 EKSSELMSDLIALKS
L373 SSELMSDLIALKSKT
T380 LIALKSKTDSSLTKL
S382 ALKSKTDSSLTKLEE
S383 LKSKTDSSLTKLEET
T390 SLTKLEETKPEIAER
K391 LTKLEETKPEIAERR
- gap
- gap
- gap
- gap
- gap
T428-p LRHVPDVtPKADKES
- gap
S435 tPKADKESPPISVPK
S439 DKESPPISVPKWLDP
T452 DPKSACETPSSSSLD
S454 KSACETPSSSSLDDY
S457 CETPSSSSLDDYMKC
Y461 SSSSLDDYMKCFKTP
K463 SSLDDYMKCFKTPVV
K466 DDYMKCFKTPVVKND
T467 DYMKCFKTPVVKNDF
K537 IGSGGSSKVFQVLNE
K545 VFQVLNEKKQINAIK
T563 LEDADSQTIESYRNE
S581 LNKLQQHSDKIIRLY
S617 SWLKKKKSINPWERK
T674 ANQMQPDTTSIVKDS
T675 NQMQPDTTSIVKDSQ
S676 QMQPDTTSIVKDSQV
T685 VKDSQVGTVNYMAPE
R695 YMAPEAIRDMSSSRE
S741 QHIINQVSKLHAIIN
- gap
- gap
T805 SQMARGATDEMkYVL
K809-ub RGATDEMkYVLGQLV
Y810 GATDEMkYVLGQLVG
S820-p GQLVGLNsPNSILKT
S823 VGLNsPNSILKTAKT
K826 NsPNSILKTAKTLYE
Y832 LKTAKTLYERYNCGE
Y835 AKTLYERYNCGEGQD
- gap
S844 CGEGQDSSSSKTFDK
S846 EGQDSSSSKTFDKKR
K847 GQDSSSSKTFDKKRE
T848 QDSSSSKTFDKKRER
K851 SSSKTFDKKRERK__
K852 SSKTFDKKRERK___
  TTK iso2  
A3 _____MEAEELIGSS
I7 _MEAEELIGSSVTID
T12 ELIGSSVTIDSIMSK
S15 GSSVTIDSIMSKMRD
K19 TIDSIMSKMRDIKNK
T32 NKINEDCTDELSLSK
S36 EDCTDELSLSKICAD
N76 EKNSSPLNDDLLNKL
K82 LNDDLLNKLIGRYSQ
Y127 EPDDARDYFQMAREN
K196 QLLPEEDKKSVSAST
P210 TVLSAQEPFSSSLGN
S212 LSAQEPFSSSLGNVQ
S214 AQEPFSSSLGNVQNR
H251 PQDAEVRHQNPFKQT
P254 AEVRHQNPFKQTHAA
S277 VPVNLLNSPDFYVKT
T284 SPDFYVKTDSSAVTQ
S287 FYVKTDSSAVTQLTT
R312 ILPGSRPRGSDSYEL
S316 SRPRGSDSYELRGLK
S345 EKSSELMSDLIALKS
L347 SSELMSDLIALKSKT
T354 LIALKSKTDSSLTKL
S356 ALKSKTDSSLTKLEE
S357 LKSKTDSSLTKLEET
T364 SLTKLEETKPEIAER
K365 LTKLEETKPEIAERR
- gap
- gap
- gap
- gap
- gap
T402 LRHVPDVTPKADKES
- gap
S409 TPKADKESPPISVPK
S413 DKESPPISVPKWLDP
T426 DPKSACETPSSSSLD
S428 KSACETPSSSSLDDY
S431 CETPSSSSLDDYMKC
Y435 SSSSLDDYMKCFKTP
K437 SSLDDYMKCFKTPVV
K440 DDYMKCFKTPVVKND
T441 DYMKCFKTPVVKNDF
K511 IGSGGSSKVFQVLNE
K519 VFQVLNEKKQINAIK
T537 LEDADSQTIESYRNE
S555 LNKLQQHSDKIIRLY
S591 SWLKKKKSINPWERK
T648 ANQMQPDTTSIVKDS
T649 NQMQPDTTSIVKDSQ
S650 QMQPDTTSIVKDSQV
T659 VKDSQVGTVNYMAPE
R669 YMAPEAIRDMSSSRE
S715 QHIINQVSKLHAIIN
- gap
- gap
T779 SQMARGATDEMKYVL
K783 RGATDEMKYVLGQLV
Y784 GATDEMKYVLGQLVG
S794-p GQLVGLNsPNSILKT
S797 VGLNsPNSILKTAKV
K800 NsPNSILKTAKVSAC
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
  rat

 
A3 _____MEAEELIGRN
I7 _MEAEELIGRNLTID
T12 ELIGRNLTIDSIMNK
S15 GRNLTIDSIMNKVRD
K19 TIDSIMNKVRDIKNK
T32-p NKINEDLtDELNSIK
N36 EDLtDELNSIKVCVD
N76 EKNSPPLNDALLNKL
K82 LNDALLNKLIGRYSQ
Y127 EPDDAHDYFQMAREN
K196 QLLSEEDKKSLSAST
S210 TVLTAQESFSSSLGN
S212 LTAQESFSSSLGNLQ
S214 AQESFSSSLGNLQSR
H251 PKDAEVSHRNPLKQT
P254 AEVSHRNPLKQTNKT
S277 VPVNLLNSPDYYVKT
T284 SPDYYVKTDGSAVPQ
S287 YYVKTDGSAVPQFTK
S313 IMPGSKSSGNDSYEL
S317 SKSSGNDSYELRGLK
S346 EKSSELMSDLVTLKS
L348 SSELMSDLVTLKSKT
T355 LVTLKSKTDSSLTKL
S357 TLKSKTDSSLTKLEE
S358 LKSKTDSSLTKLEEP
P365 SLTKLEEPKVRQEPE
K366 LTKLEEPKVRQEPEI
R368 KLEEPKVRQEPEIPE
R376 QEPEIPERRHKQWQS
K379 EIPERRHKQWQSARK
K379 EIPERRHKQWQSARK
P387 QWQSARKPGCGIQNS
T407 QWHVPDVTPKADKES
- gap
S414 TPKADKESPPLSVPK
S418 DKESPPLSVPKWIDP
T431 DPKSVCETPSSSSLD
S433 KSVCETPSSSSLDDY
S436 CETPSSSSLDDYMKC
Y440 SSSSLDDYMKCFKTS
K442 SSLDDYMKCFKTSVV
K445 DDYMKCFKTSVVKNE
T446 DYMKCFKTSVVKNEF
K516 IGSGGSSKVFQVLNE
K524 VFQVLNEKKQINAIK
T542 LEDADSQTIDSYRNE
S560 LNKLQQHSDKIIRLY
S596 SWLKKKKSINPWERK
T653 ANQMQPDTTSIVKDS
T654 NQMQPDTTSIVKDSQ
S655 QMQPDTTSIVKDSQV
T664 VKDSQVGTVNYMAPE
R674 YMAPEAIRDMSSSRE
S720 QHIINQVSKLHAIID
- gap
- gap
T784 SQMAKETTDEMKYML
K788 KETTDEMKYMLGQLV
Y789 ETTDEMKYMLGQLVC
S799 GQLVCLNSPNSILKA
S802 VCLNSPNSILKAAKT
K805 NSPNSILKAAKTLYE
Y811 LKAAKTLYERYNCGE
Y814 AKTLYERYNCGESQA
N815 KTLYERYNCGESQAP
S823 CGESQAPSSSKTSDK
S825 ESQAPSSSKTSDKKR
K826 SQAPSSSKTSDKKRE
T827 QAPSSSKTSDKKRER
K830 SSSKTSDKKRERE__
K831 SSKTSDKKRERE___
  frog

 
D3 _____MDDEDISERK
S7 _MDDEDISERKLKIA
K12 DISERKLKIASILDR
S15 ERKLKIASILDRVKS
R19 KIASILDRVKSFKTK
T34 YGTDDNWTDELTFSK
T38 DNWTDELTFSKSSAD
I80 ENTGLPQIDPQLLNK
K87 IDPQLLNKLIDNYSQ
Q132 DLDEAREQFQFARLN
K201 QLISDEDKENLAVSS
T215 SSHINQGTASFQNLA
S217 HINQGTASFQNLALG
Q219 NQGTASFQNLALGNP
G259 PEDFEDIGRKPLLNM
P262 FEDIGRKPLLNMSAK
S283 VPVQPATSPDTRTRK
S291 PDTRTRKSDGSGSSS
S294 RTRKSDGSGSSSVVK
A345 ILPHEEQANEDSLDM
S349 EEQANEDSLDMKTPS
S379 NEDLILGSTSTAVLN
- gap
- gap
- gap
S391 VLNPQENSKPSESAP
S396 ENSKPSESAPPLLPN
R407 LLPNINARLSDTTKP
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S459 NPGSRRVSPPAPSLS
S464 RVSPPAPSLSKCDPV
T476 DPVFVCGTPVNKSQE
- gap
S481 CGTPVNKSQEDYMNC
Y485 VNKSQEDYMNCFRTP
N487 KSQEDYMNCFRTPVV
R490 EDYMNCFRTPVVKTN
T491 DYMNCFRTPVVKTNL
K559 IGTGGSSKVFQVMDD
K567 VFQVMDDKKHLYAIK
T585 LEEADQQTIESYQNE
- gap
T639 TWLRKKKTINPWERK
- gap
T697 NQIQPDVTSIVKDSQ
S698 QIQPDVTSIVKDSQV
T707 VKDSQVGTINYMPPE
R717 YMPPESIRDTTSYAE
- gap
- gap
T828 DQQVQKETTEEMKRI
T829 QQVQKETTEEMKRIL
K833 KETTEEMKRILGQLI
R834 ETTEEMKRILGQLIG
S844-p GQLIGLNsPNSISRA
S847 IGLNsPNSISRAAKN
R850 NsPNSISRAAKNLYD
Y856 SRAAKNLYDQFNSGR
F859 AKNLYDQFNSGRSLD
N860 KNLYDQFNSGRSLDL
S868 SGRSLDLSTLGTTVS
L870 RSLDLSTLGTTVSQN
G871 SLDLSTLGTTVSQNT
T872 LDLSTLGTTVSQNTR
S875 STLGTTVSQNTRTTK
Q876 TLGTTVSQNTRTTK_
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