Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Protein Page:
NFH (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
NFH Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. Belongs to the intermediate filament family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Cytoskeletal protein
Cellular Component: mitochondrion; axon; neurofilament
Molecular Function: dynein binding; structural constituent of cytoskeleton; kinesin binding; microtubule binding
Biological Process: cell death; axonogenesis; neurofilament cytoskeleton organization and biogenesis; cell projection biogenesis; microtubule cytoskeleton organization and biogenesis; intermediate filament bundle assembly; peripheral nervous system neuron axonogenesis
Reference #:  P12036 (UniProtKB)
Alt. Names/Synonyms: 200 kDa neurofilament protein; KIAA0845; NEFH; Neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide; neurofilament, heavy polypeptide 200kDa; NF-H; NFH
Gene Symbols: NEFH
Molecular weight: 112,479 Da
Basal Isoelectric point: 5.99  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

NFH

Protein Structure Not Found.


STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  DISEASE  |  Source  |  InnateDB  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene


Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S39 GGAGGTRSAAGSSSG
1 0 T53 GFHSWTRTSVSSVsA
1 0 S54 FHSWTRTSVSSVsAs
1 0 S56 SWTRTSVSSVsAsPS
0 2 S59-p RTSVSSVsAsPSRFR
0 7 S61-p SVSSVsAsPSRFRGA
0 1 S72 FRGAGAASSTDSLDT
0 1 T74 GAGAASSTDSLDTLS
0 1 S76 GAASSTDSLDTLSNG
0 1 T79 SSTDSLDTLSNGPEG
0 1 K98 VATSRSEKEQLQALN
0 2 S124 QLEAHNRSLEGEAAA
0 36 Y229 ALQEECGYLRRHHQE
0 3 K264 AETRDALKCDVTSAL
0 1 K305 DRLSEAAKVNTDAMR
0 61 Y321 AQEEITEYRRQLQAR
0 4 Y385-p MAAQLREyQDLLNVK
0 2 Y402 LDIEIAAYRKLLEGE
0 11 K404 IEIAAYRKLLEGEEC
0 14 I418 CRIGFGPIPFSLPEG
0 5 S421 GFGPIPFSLPEGLPK
0 1 P423 GPIPFSLPEGLPKIP
0 2 K428 SLPEGLPKIPsVSTH
0 2 S431-p EGLPKIPsVSTHIKV
0 1 S433 LPKIPsVSTHIKVKS
0 1 S440 STHIKVKSEEKIKVV
0 1 K445 VKSEEKIKVVEKSEK
0 4 T501-p AEGGEEEtKsPPAEE
2 8 S503-p GGEEEtKsPPAEEAA
1 7 S511-p PPAEEAAsPEKEAKs
0 1 S518-p sPEKEAKsPVKEEAK
0 9 S526-p PVKEEAKsPAEAKsP
0 18 S532-p KsPAEAKsPEKEEAK
0 13 S540-p PEKEEAKsPAEVKsP
0 14 S546-p KsPAEVKsPEKAKsP
0 4 S552-p KsPEKAKsPAKEEAK
0 7 S560-p PAKEEAKsPPEAKsP
0 10 S566-p KsPPEAKsPEKEEAK
0 8 - gap
0 16 S574-p PEKEEAKsPAEVKsP
0 15 S580-p KsPAEVKsPEKAKSP
0 11 S586 KsPEKAKSPAKEEAK
0 9 - gap
0 9 S594-p PAKEEAKsPAEAKsP
0 18 S600-p KsPAEAKsPEKAKsP
0 14 S606-p KsPEKAKsPVKEEAK
0 10 - gap
0 12 S614-p PVKEEAKsPAEAKsP
0 15 S620-p KsPAEAKsPVKEEAK
0 11 - gap
0 14 S628-p PVKEEAKsPAEVKsP
0 3 - gap
0 12 S634-p KsPAEVKsPEKAKsP
0 9 S640-p KsPEKAKsPTKEEAK
0 10 T642 PEKAKsPTKEEAKsP
0 13 S648-p PTKEEAKsPEKAKsP
0 11 S654-p KsPEKAKsPEKAKsP
0 10 S660-p KsPEKAKsPEKEEAK
0 6 - gap
0 13 S668-p PEKEEAKsPEKAKsP
0 14 S674-p KsPEKAKsPVKAEAK
0 9 - gap
0 6 - gap
0 8 - gap
0 10 - gap
0 11 - gap
0 11 - gap
0 14 S682-p PVKAEAKsPEKAKsP
0 10 S688-p KsPEKAKsPVKAEAK
0 10 - gap
0 19 S696-p PVKAEAKsPEKAKsP
0 18 S702-p KsPEKAKsPVKEEAK
0 16 A708 KsPVKEEAKsPEKAK
0 12 S710-p PVKEEAKsPEKAKsP
0 6 S716-p KsPEKAKsPVKEEAK
0 4 - gap
0 7 S724-p PVKEEAKsPEKAKsP
0 7 S730-p KsPEKAKsPVKEEAK
0 2 T738-p PVKEEAKtPEKAKsP
0 6 S744-p KtPEKAKsPVKEEAK
0 2 A750 KsPVKEEAKsPEKAK
0 3 S752-p PVKEEAKsPEKAKsP
0 2 S758-p KsPEKAKsPEKAKTL
0 9 S769-p AKTLDVKsPEAKTPA
0 4 T774 VKsPEAKTPAKEEAR
0 1 S782-p PAKEEARsPADKFPE
0 1 A784 KEEARsPADKFPEKA
0 7 S793-p KFPEKAKsPVKEEVK
0 7 S801-p PVKEEVKsPEKAKSP
0 11 S828-p PKKEEVKsPVKEEEK
0 1 P886 KVEEKKEPAVEKPKE
0 1 V888 EEKKEPAVEKPKESK
0 2 T911-p EDKKKVPtPEKEAPA
0 1 P912 DKKKVPtPEKEAPAK
0 1 A935 PKEKTEVAKKEPDDA
0 2 S948-p DAKAKEPsKPAEKKE
  mouse

► Hide Isoforms
 
S39-gl AGPGGTRsAAGSSSG
T53 GFHSWARTSVSSVsA
S54 FHSWARTSVSSVsAs
S56 SWARTSVSSVsAsPS
S59-p RTSVSSVsAsPSRFR
S61-p SVSSVsAsPSRFRGA
S70-p SRFRGAAsStDsLDt
T72-p FRGAAsStDsLDtLS
S74-p GAAsStDsLDtLSNG
T77-p sStDsLDtLSNGPEG
K96-u AVAARSEkEQLQALN
S122-p QLEAHNRsLEGEAAA
Y227 ALQEECGYLRRHHQE
K262-u AEARDALkCDVTSAL
K303-u DRLSEAAkVNTDAMR
Y319 AQEEITEYRRQLQAR
Y383 MAAQLREYQDLLNVK
Y400 LDIEIAAYRkLLEGE
K402-u IEIAAYRkLLEGEEC
S416-p CRIGFGPsPFsLtEG
S419-p GFGPsPFsLtEGLPk
T421-p GPsPFsLtEGLPkIP
K426-u sLtEGLPkIPsIsTH
S429-p EGLPkIPsIsTHIKV
S431-gl LPkIPsIsTHIKVKs
S438-p sTHIKVKsEEMIkVV
K443-u VKsEEMIkVVEKSEK
T499-p EEEGAAAtsPPAEEA
S500-p EEGAAAtsPPAEEAA
S508-p PPAEEAAsPEKETKS
S515 sPEKETKSRVKEEAK
S523-p RVKEEAKsPGEAKsP
S529-p KsPGEAKsPGEAKsP
S535-p KsPGEAKsPAEAKsP
S541-p KsPAEAKsPGEAKsP
S547-p KsPGEAKsPGEAKsP
S553-p KsPGEAKsPGEAKsP
S559-p KsPGEAKsPAEPKsP
S565-p KsPAEPKsPAEPKsP
S571-p KsPAEPKsPAEAKsP
S577-p KsPAEAKsPAEPKsP
S583-p KsPAEPKsPATVKsP
S589-p KsPATVKsPGEAKsP
S595-p KsPGEAKsPSEAKsP
S601-p KsPSEAKsPAEAKsP
S607-p KsPAEAKsPAEAKsP
S613-p KsPAEAKsPAEAKsP
S619-p KsPAEAKsPAEAKsP
S625-p KsPAEAKsPAEAKsP
S631-p KsPAEAKsPAEAKsP
S637-p KsPAEAKsPAtVKsP
T640-p AEAKsPAtVKsPGEA
S643-p KsPAtVKsPGEAKsP
S649-p KsPGEAKsPSEAKsP
S655-p KsPSEAKsPAEAKsP
S661-p KsPAEAKsPAEAKsP
S667-p KsPAEAKsPAEAKsP
S673-p KsPAEAKsPAEVKsP
S679-p KsPAEVKsPGEAKsP
S685-p KsPGEAKsPAEPKsP
S691-p KsPAEPKsPAEAKsP
S697-p KsPAEAKsPAEVKsP
S703-p KsPAEVKsPAEAKsP
S709-p KsPAEAKsPAEVKsP
S715-p KsPAEVKsPGEAKsP
S721-p KsPGEAKsPAAVKsP
S727-p KsPAAVKsPAEAKsP
S733-p KsPAEAKsPAAVKsP
S739-p KsPAAVKsPGEAKsP
S745-p KsPGEAKsPGEAKsP
S751-p KsPGEAKsPAEAKsP
S757-p KsPAEAKsPAEAKsP
S763-p KsPAEAKsPIEVKsP
S769-p KsPIEVKsPEKAKtP
T775-p KsPEKAKtPVKEGAK
S783-p PVKEGAKsPAEAKsP
S789-p KsPAEAKsPEKAKsP
S795-p KsPEKAKsPVKEDIK
P803 PVKEDIKPPAEAKsP
S809-p KPPAEAKsPEKAKsP
S815-p KsPEKAKsPVKEGAK
V817 PEKAKsPVKEGAKPP
P823 PVKEGAKPPEKAKPL
S834-p AKPLDVKsPEAQtPV
T839-p VKsPEAQtPVQEEAK
H847 PVQEEAKHPtDIRPP
T849-p QEEAKHPtDIRPPEQ
S859-p RPPEQVKsPAKEKAK
S867-p PAKEKAKsPEKEEAK
S888-p PKKEEVKsPVKEEVK
T939-p KVEEKKEtPtEKPKD
T941-p EEKKEtPtEKPKDST
A964 GEKKKAVAsEEETPA
S965-p EKKKAVAsEEETPAK
T988-p PKEKTETtKTEAEDT
S1001-p DTKAKEPsKPTETEK
  NFH iso3  
S39 AGPGGTRSAAGSSSG
T53 GFHSWARTSVSSVSA
S54 FHSWARTSVSSVSAS
S56 SWARTSVSSVSASPS
S59 RTSVSSVSASPSRFR
S61 SVSSVSASPSRFRGA
S70 SRFRGAASSTDSLDT
T72 FRGAASSTDSLDTLS
S74 GAASSTDSLDTLSNG
T77 SSTDSLDTLSNGPEG
K96 AVAARSEKEQLQALN
S122 QLEAHNRSLEGEAAA
Y227 ALQEECGYLRRHHQE
K262 AEARDALKCDVTSAL
K303 DRLSEAAKVNTDAMR
Y319 AQEEITEYRRQLQAR
Y383 MAAQLREYQDLLNVK
Y400 LDIEIAAYRKLLEGE
K402 IEIAAYRKLLEGEEC
S416 CRIGFGPSPFSLTEG
S419 GFGPSPFSLTEGLPK
T421 GPSPFSLTEGLPKIP
K426 SLTEGLPKIPSISTH
S429 EGLPKIPSISTHIKV
S431 LPKIPSISTHIKVKS
S438 STHIKVKSEEMIKVV
K443 VKSEEMIKVVEKSEK
T499 EEEGAAATSPPAEEA
S500 EEGAAATSPPAEEAA
S508 PPAEEAASPEKETKS
S515 SPEKETKSRVKEEAK
S523 RVKEEAKSPGEAKSP
S529 KSPGEAKSPGEAKSP
S541 KSPGEAKSPAEAKSP
S547 KSPAEAKSPGEAKSP
S553 KSPGEAKSPREAKSP
S559 KSPREAKSPGEAKSP
S565 KSPGEAKSPAEPKSP
S571 KSPAEPKSPAEPKSP
S577 KSPAEPKSPAEAKSP
S583 KSPAEAKSPAEPKSP
S589 KSPAEPKSPATVKSP
S595 KSPATVKSPGEAKSP
S601 KSPGEAKSPSEAKSP
S607 KSPSEAKSPAEAKSP
S613 KSPAEAKSPAEAKSP
S619 KSPAEAKSPAEAKSP
S625 KSPAEAKSPAEAKSP
S631 KSPAEAKSPAEAKSP
S637 KSPAEAKSPAEAKSP
S643 KSPAEAKSPATVKSP
T646 AEAKSPATVKSPGEA
S649 KSPATVKSPGEAKSP
S655 KSPGEAKSPSEAKSP
S661 KSPSEAKSPAEAKSP
S667 KSPAEAKSPAEAKSP
S673 KSPAEAKSPAEAKSP
S679 KSPAEAKSPAEVKSP
S685 KSPAEVKSPGEAKSP
S691 KSPGEAKSPAEPKsP
S697-p KSPAEPKsPAEAKsP
S703-p KsPAEAKsPAAVKsP
- gap
- gap
- gap
- gap
S709-p KsPAAVKsPAEAKSP
S715 KsPAEAKSPAAVKSP
S721 KSPAAVKSPGEAKSP
S727 KSPGEAKSPGEAKSP
S733 KSPGEAKSPAEAKSP
S739 KSPAEAKSPAEAKSP
S745 KSPAEAKSPIEVKSP
S751 KSPIEVKSPEKAKTP
T757 KSPEKAKTPVKEGAK
S765 PVKEGAKSPAEAKSP
S771 KSPAEAKSPEKAKSP
S777 KSPEKAKSPVKEDIK
P785 PVKEDIKPPAEAKSP
S791 KPPAEAKSPEKAKsP
S797-p KSPEKAKsPMKEGAK
M799 PEKAKsPMKEGAKPP
P805 PMKEGAKPPEKAKPL
S816 AKPLDVKSPEAQTPV
T821 VKSPEAQTPVQEEAN
D829 PVQEEANDPTDIRPP
T831 QEEANDPTDIRPPEQ
S841 RPPEQVKSPAKEKAK
S849 PAKEKAKSPEKEEAK
S870 PKKEEVKSPVKEEVK
T921 KVEEKKETPTEKPKD
T923 EEKKETPTEKPKDST
A946 GEKKKAVASEEETPA
S947 EKKKAVASEEETPAK
T970 PKEKTETTKTEAEDT
S983 DTKAKEPSKPTETEK
  rat

 
S39 AGAGGTRSAAGSSSG
T53-gl GFHSWARtsVsSVSA
S54-gl FHSWARtsVsSVSAS
S56-gl SWARtsVsSVSASPS
S59 RtsVsSVSASPSRFR
S61 sVsSVSASPSRFRGA
S70 SRFRGAASSTDSLDT
T72 FRGAASSTDSLDTLS
S74 GAASSTDSLDTLSNG
T77 SSTDSLDTLSNGPEG
K95 AVAARSEKEQLQALN
T121 QLEAHNRTLEGEAAA
Y225-p ALQEECGyLRRHHQE
K260 AEARDALKCDVTSAL
K301 DRLSEAAKVNTDAMR
Y317-p AQEEITEyRRQLQAR
Y381-p MAAQLREyQDLLNVK
Y398-p LDIEIAAyRKLLEGE
K400 IEIAAyRKLLEGEEC
S414 CRIGFGPSPFSLTEG
S417 GFGPSPFSLTEGLPK
T419 GPSPFSLTEGLPKIP
K424 SLTEGLPKIPSMSTH
S427 EGLPKIPSMSTHIKV
S429 LPKIPSMSTHIKVKS
S436 STHIKVKSEEKIKVV
K441 VKSEEKIKVVEKSEK
T491 EGGEEAATTsPPAEE
S493-p GEEAATTsPPAEEAA
S501-p PPAEEAAsPEKETKS
S508 sPEKETKSPVKEEAK
S516 PVKEEAKSPAEAKSP
S522 KSPAEAKSPAEAKSP
S534 KSPAEAKSPAEVKSP
S540 KSPAEVKSPAEVKSP
S546 KSPAEVKSPAEAKSP
S552 KSPAEAKSPAEAKSP
S558 KSPAEAKSPAEVKSP
S564 KSPAEVKSPAEVKSP
S570 KSPAEVKSPAEAKSP
S576 KSPAEAKSPAEAKSP
S582 KSPAEAKSPAEVKSP
S594 KSPATVKSPGEAKSP
S600 KSPGEAKSPAEAKSP
S606 KSPAEAKSPAEVKSP
S612 KSPAEVKSPVEAKSP
S618 KSPVEAKSPAEAKSP
S624 KSPAEAKSPASVKSP
S630 KSPASVKSPGEAKSP
S636 KSPGEAKSPAEAKSP
S642 KSPAEAKSPAEVKSP
- gap
S648 KSPAEVKSPATVKSP
S654 KSPATVKSPVEAKSP
S660 KSPVEAKSPAEVKSP
S666 KSPAEVKSPVTVKSP
S672 KSPVTVKSPAEAKSP
S678 KSPAEAKSPVEVKSP
S684 KSPVEVKSPASVKSP
S690 KSPASVKSPSEAKSP
S696 KSPSEAKSPAGAKSP
S702 KSPAGAKSPAEAKSP
S708 KSPAEAKSPVVAKSP
S714 KSPVVAKSPAEAKSP
- gap
- gap
S720 KSPAEAKSPAEAKPP
P726 KSPAEAKPPAEAKSP
S732 KPPAEAKSPAEAKSP
S738 KSPAEAKSPAEAKSP
S744 KSPAEAKSPAEAKSP
S750 KSPAEAKSPAEAKSP
S756 KSPAEAKSPVEVKsP
S762-p KSPVEVKsPEKAKSP
S768 KsPEKAKSPVKEGAK
S776 PVKEGAKSLAEAKSP
S782 KSLAEAKSPEKAKSP
S788 KSPEKAKSPVKEEIK
P796 PVKEEIKPPAEVKSP
S802 KPPAEVKSPEKAKSP
S808 KSPEKAKSPMKEEAK
M810 PEKAKSPMKEEAKSP
S816 PMKEEAKSPEKAKTL
S827 AKTLDVKSPEAKTPA
T832 VKSPEAKTPAKEEAK
R840 PAKEEAKRPADIRSP
A842 KEEAKRPADIRSPEQ
S852 RSPEQVKSPAKEEAK
S860 PAKEEAKSPEKEETR
S880-p PKKEEVKsPVEEVKA
P929 PKAEEKEPLTEKPKD
T931 AEEKEPLTEKPKDSP
A953 AKEKKAAAPEEETPA
P954 KEKKAAAPEEETPAK
- gap
S983 DAKAKEPSKPSEKEK
Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.