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Protein Page:
Cdc27 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
g O-GlcNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
Cdc27 Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The APC/C is composed of at least 12 subunits. Interacts with RB. Interacts with FAM168B/MANI. Belongs to the APC3/CDC27 family. Note: This description may include information from UniProtKB.
Protein type: Cell cycle regulation
Cellular Component: nucleoplasm; centrosome; anaphase-promoting complex; spindle microtubule; cytoplasm; nucleolus; spindle; cytosol; nucleus
Molecular Function: protein binding; protein phosphatase binding
Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell proliferation; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle; cell cycle checkpoint; mitotic metaphase/anaphase transition
Reference #:  P30260 (UniProtKB)
Alt. Names/Synonyms: ANAPC3; anaphase promoting complex subunit 3; anaphase-promoting complex, protein 3; APC3; CDC27; CDC27 homolog; CDC27Hs; cell division cycle 27 homolog (S. cerevisiae); cell division cycle protein 27; Cell division cycle protein 27 homolog; D0S1430E; D17S978E; H-NUC; HNUC; nuc2 homolog
Gene Symbols: CDC27
Molecular weight: 91,867 Da
Basal Isoelectric point: 6.59  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Cdc27
Protein Structure Not Found.


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Sites Implicated In
cell growth, altered: S154‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


  MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


        human

► Hide Isoforms 		 
0 1 T68-p LKGHSCTtPQCKYLL
0 1 K77-a QCKYLLAkCCVDLSK
0 1 K99-u LSGGVFNkQKSHDDI
1 0 S154-p FLWSPFEsLCEIGEK
0 1 S183-p FSNCLPNsCTTQVPN
0 1 S192-p TTQVPNHsLSHRQPE
0 1 T203-p RQPETVLtEtPQDtI
0 5 T205-p PETVLtEtPQDtIEL
0 2 T209-p LtEtPQDtIELNRLN
0 2 S220-p NRLNLESsNsKYSLN
0 2 S222-p LNLESsNsKYSLNTD
0 1 S241-p YIDSAVIsPDtVPLG
1 1 T244-p SAVIsPDtVPLGTGT
0 1 S252-p VPLGTGTsILSKQVQ
0 1 T264-p QVQNKPKtGRSLLGG
0 1 S276-p LGGPAALsPLtPSFG
0 1 T279-p PAALsPLtPSFGILP
0 1 T289-p FGILPLEtPsPGDGs
0 1 S291-p ILPLEtPsPGDGsYL
0 1 S296-p tPsPGDGsYLQNYTN
0 1 T304-p YLQNYTNtPPVIDVP
0 2 S312-p PPVIDVPstGAPSKk
0 2 T313-p PVIDVPstGAPSKkS
0 1 K319-u stGAPSKkSVARIGQ
0 1 S320 tGAPSKkSVARIGQT
0 1 T329-p ARIGQTGtkSVFsQs
0 1 K330-u RIGQTGtkSVFsQsG
0 4 S334-p TGtkSVFsQsGNsRE
0 1 S336-p tkSVFsQsGNsREVt
0 3 S339-p VFsQsGNsREVtPIL
0 1 T343-p sGNsREVtPILAQTQ
0 1 T359-p SGPQTSTtPQVLsPt
0 2 S364-p STtPQVLsPtItsPP
0 4 T366-p tPQVLsPtItsPPNA
0 1 T368-p QVLsPtItsPPNALP
0 2 S369-p VLsPtItsPPNALPR
0 3 S378-p PNALPRRssRLFtsD
1 13 S379-p NALPRRssRLFtsDs
0 3 T383-p RRssRLFtsDssttK
0 3 S384-p RssRLFtsDssttKE
0 4 S386-p sRLFtsDssttKENs
0 2 S387-p RLFtsDssttKENsK
0 1 T388-p LFtsDssttKENsKK
0 2 T389-p FtsDssttKENsKKL
0 1 S393-p ssttKENsKKLKMKF
0 1 K394 sttKENsKKLKMKFP
0 1 K395 ttKENsKKLKMKFPP
0 1 K397 KENsKKLKMKFPPKI
0 1 K415-u KTKSKTNkGGITQPN
1 6 S426-p TQPNINDsLEItkLD
0 3 T430-p INDsLEItkLDssII
0 1 K431-u NDsLEItkLDssIIs
0 3 S434-p LEItkLDssIIsEGk
0 7 S435-p EItkLDssIIsEGkI
0 6 S438-p kLDssIIsEGkIStI
0 2 K441-u ssIIsEGkIStItPQ
0 1 T444-p IsEGkIStItPQIQA
1 6 T446-p EGkIStItPQIQAFN
0 1 S553-p LQKDVALsVLSkDLT
0 1 K557-u VALsVLSkDLTDMDK
0 1 S578-p CAAGNCFsLQREHDI
0 1 K588-u REHDIAIkFFQRAIQ
0 1 T691-p KSEKALDtLNkAIVI
0 2 K694-u KALDtLNkAIVIDPK
0 1 K718-u SVLFANEkYkSALQE
0 1 K720-u LFANEkYkSALQELE
0 1 K730-u LQELEELkQIVPkES
0 1 K735-u ELkQIVPkESLVyFL
0 12 Y740-p VPkESLVyFLIGKVY
1 0 S821-p TQLHAAEsDEF____
  Cdc27 iso1  
T68 LKGHSCTTPQCKYLL
K77 QCKYLLAKCCVDLSK
K99 LSGGVFNKQKSHDDI
S154 FLWSPFESLCEIGEK
S183 FSNCLPNSCTTQVPN
S192 TTQVPNHSLSHRQPE
T203 RQPETVLTETPQDTI
T205 PETVLTETPQDTIEL
T209 LTETPQDTIELNRLN
S220 NRLNLESSNSKYSLN
S222 LNLESSNSKYSLNTD
S241 YIDSAVISPDTVPLG
T244 SAVISPDTVPLGTGT
S252 VPLGTGTSILSKQVQ
T264 QVQNKPKTGRSLLGG
S276 LGGPAALSPLTPSFG
T279 PAALSPLTPSFGILP
T289 FGILPLETPSPGDGS
S291 ILPLETPSPGDGSYL
S296 TPSPGDGSYLQNYTN
T304 YLQNYTNTPPVIDVP
S312 PPVIDVPStGAPSKK
T313-p PVIDVPStGAPSKKT
K319 StGAPSKKTFRVLQs
S326-p KTFRVLQsVARIGQT
T335 ARIGQTGTKSVFSQS
K336 RIGQTGTKSVFSQSG
S340 TGTKSVFSQSGNSRE
S342 TKSVFSQSGNSREVT
S345 VFSQSGNSREVTPIL
T349 SGNSREVTPILAQTQ
T365 SGPQTSTTPQVLSPT
S370 STTPQVLSPTITSPP
T372 TPQVLSPTITSPPNA
T374 QVLSPTITSPPNALP
S375 VLSPTITSPPNALPR
S384 PNALPRRSSRLFTSD
S385 NALPRRSSRLFTSDS
T389 RRSSRLFTSDSSTTK
S390 RSSRLFTSDSSTTKE
S392 SRLFTSDSSTTKENS
S393 RLFTSDSSTTKENSK
T394 LFTSDSSTTKENSKK
T395 FTSDSSTTKENSKKL
S399 SSTTKENSKKLKMKF
K400 STTKENSKKLKMKFP
K401 TTKENSKKLKMKFPP
K403 KENSKKLKMKFPPKI
K421 KTKSKTNKGGITQPN
S432 TQPNINDSLEITKLD
T436 INDSLEITKLDSSII
K437 NDSLEITKLDSSIIS
S440 LEITKLDSSIISEGK
S441 EITKLDSSIISEGKI
S444 KLDSSIISEGKISTI
K447 SSIISEGKISTITPQ
T450 ISEGKISTITPQIQA
T452 EGKISTITPQIQAFN
S559 LQKDVALSVLSKDLT
K563 VALSVLSKDLTDMDK
S584 CAAGNCFSLQREHDI
K594 REHDIAIKFFQRAIQ
T697 KSEKALDTLNKAIVI
K700 KALDTLNKAIVIDPK
K724 SVLFANEKYKSALQE
K726 LFANEKYKSALQELE
K736 LQELEELKQIVPKES
K741 ELKQIVPKESLVYFL
Y746 VPKESLVYFLIGKVY
S827 TQLHAAESDEF____
  mouse

► Hide Isoforms 		 
T68 LKGHSCTTPQCKYLL
K77 QCKYLLAKCCVDLSK
K99 LSGGVFNKQKSHDDL
S154 FLWSPFESLCEIGEK
T183 FSSCLPNTCTTLVSN
S192 TTLVSNHSLSHRQPE
T203 RQPETVLTETPQDTI
T205 PETVLTETPQDTIEL
T209 LTETPQDTIELNRLN
S220 NRLNLESSNSKYSLN
S222 LNLESSNSKYSLNTD
S241 YIDSTVISPDNVPLG
N244 STVISPDNVPLGPGT
A252 VPLGPGTAILSKQVQ
T264 QVQNKPKTGRSLLGG
S276 LGGPTALSPLTPSFG
T279 PTALSPLTPSFGILP
T289 FGILPLETPSPGDGS
S291 ILPLETPSPGDGSYL
S296 TPSPGDGSYLQNYTN
T304 YLQNYTNTPSVIDVA
P312 PSVIDVAPTGAPTKK
T313 SVIDVAPTGAPTKKS
K319 PTGAPTKKSVARMGQ
S320 TGAPTKKSVARMGQT
T329 ARMGQTGTKSVFSQS
K330 RMGQTGTKSVFSQSG
S334 TGTKSVFSQSGNSRE
S336 TKSVFSQSGNSREVT
S339 VFSQSGNSREVTPVL
T343 SGNSREVTPVLVAQT
T360 SGPQTSTTPQVLSPT
S365 STTPQVLSPTITSPP
T367 TPQVLSPTITSPPNA
T369 QVLSPTITSPPNALP
S370 VLSPTITSPPNALPR
S379-p PNALPRRssRLFTSD
S380-p NALPRRssRLFTSDS
T384 RRssRLFTSDSSTTK
S385 RssRLFTSDSSTTKE
S387 sRLFTSDSSTTKENS
S388 RLFTSDSSTTKENSk
T389 LFTSDSSTTKENSkk
T390 FTSDSSTTKENSkkL
S394 SSTTKENSkkLkMKF
K395-a STTKENSkkLkMKFP
K396-a TTKENSkkLkMKFPP
K398-a KENSkkLkMKFPPKI
K416 KTKSKTNKGGLTQPS
S427 TQPSINDSLEITKLD
T431 INDSLEITKLDSSII
K432 NDSLEITKLDSSIIS
S435 LEITKLDSSIISEGK
S436 EITKLDSSIISEGKI
S439 KLDSSIISEGKITTV
K442 SSIISEGKITTVTPQ
T445 ISEGKITTVTPQIQA
T447 EGKITTVTPQIQAFN
S554 LQKDVALSVLSKDLT
K558 VALSVLSKDLTDMDK
S579 CAAGNCFSLQREHDI
K589 REHDIAIKFFQRAIQ
T692 KSEKALDTLNKAIVI
K695 KALDTLNKAIVIDPK
K719 SVLFANEKYKSALQE
K721 LFANEKYKSALQELE
K731 LQELEELKQIVPKES
K736 ELKQIVPKESLVYFL
Y741 VPKESLVYFLIGKVY
S822 TQLHAAESDEF____
  Cdc27 iso2  
T49 LKGHSCTTPQCKYLL
K58 QCKYLLAKCCVDLSK
K80 LSGGVFNKQKSHDDL
S135 FLWSPFESLCEIGEK
T164 FSSCLPNTCTTLVSN
S173 TTLVSNHSLSHRQPE
T184 RQPETVLTETPQDTI
T186 PETVLTETPQDTIEL
T190 LTETPQDTIELNRLN
S201 NRLNLESSNSKYSLN
S203 LNLESSNSKYSLNTD
S222 YIDSTVISPDNVPLG
N225 STVISPDNVPLGPGT
A233 VPLGPGTAILSKQVQ
T245 QVQNKPKTGRSLLGG
S257 LGGPTALSPLTPSFG
T260 PTALSPLTPSFGILP
T270 FGILPLETPSPGDGS
S272 ILPLETPSPGDGSYL
S277 TPSPGDGSYLQNYTN
T285 YLQNYTNTPSVIDVA
P293 PSVIDVAPNGAPTKK
N294 SVIDVAPNGAPTKKS
K300 PNGAPTKKSVARMGQ
S301 NGAPTKKSVARMGQT
T310 ARMGQTGTKSVFSQS
K311 RMGQTGTKSVFSQSG
S315 TGTKSVFSQSGNSRE
S317 TKSVFSQSGNSREVT
S320 VFSQSGNSREVTPVL
T324 SGNSREVTPVLVAQT
T341 SGPQTSTTPQVLSPT
S346 STTPQVLSPTITSPP
T348 TPQVLSPTITSPPNA
T350 QVLSPTITSPPNALP
S351 VLSPTITSPPNALPR
S360 PNALPRRSSRLFTSD
S361 NALPRRSSRLFTSDS
T365 RRSSRLFTSDSSTTK
S366 RSSRLFTSDSSTTKE
S368 SRLFTSDSSTTKENS
S369 RLFTSDSSTTKENSK
T370 LFTSDSSTTKENSKK
T371 FTSDSSTTKENSKKL
S375 SSTTKENSKKLKMKF
K376 STTKENSKKLKMKFP
K377 TTKENSKKLKMKFPP
K379 KENSKKLKMKFPPKI
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- under review  
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- under review  
  rat

 
T68 LKGHSCTTPQCKYLL
K77 QCKYLLAKCCVDLSK
K99 LSGGVFNKQKSHDDL
S154 FLWSPFESLCEIGEK
T183 FSSCLPNTCTALVSN
S192 TALVSNHSLSHRQPE
T203 RQPETVLTETPQDTI
T205 PETVLTETPQDTIEL
T209 LTETPQDTIELNRLN
S220 NRLNLESSNSKYSLN
S222 LNLESSNSKYSLNTD
S241 YIDSAVISPDNVPLG
N244 SAVISPDNVPLGTGP
S252 VPLGTGPSILSKQVQ
T264 QVQNKPKTGRSLLGG
S276 LGGPTALSPLTPSFG
T279 PTALSPLTPSFGILP
T289 FGILPLETPSPGDGS
S291 ILPLETPSPGDGSYL
S296 TPSPGDGSYLQNYTN
T304 YLQNYTNTPSVIDVP
S312 PSVIDVPSTGAPTKK
T313 SVIDVPSTGAPTKKS
K319 STGAPTKKSVARMGH
S320 TGAPTKKSVARMGHT
A329 ARMGHTGAKSVFSQS
K330 RMGHTGAKSVFSQSG
S334 TGAKSVFSQSGNSRE
S336 AKSVFSQSGNSREAT
S339 VFSQSGNSREATPVL
T343 SGNSREATPVLVAQT
T360 SGPQTSTTPQVLSPT
S365 STTPQVLSPTITSPP
T367 TPQVLSPTITSPPNA
T369 QVLSPTITSPPNALP
S370 VLSPTITSPPNALPR
S379 PNALPRRSsRLFTSD
S380-p NALPRRSsRLFTSDS
T384 RRSsRLFTSDSSTTK
S385 RSsRLFTSDSSTTKE
S387 sRLFTSDSSTTKENS
S388 RLFTSDSSTTKENSK
T389 LFTSDSSTTKENSKK
T390 FTSDSSTTKENSKKL
S394 SSTTKENSKKLKMKF
K395 STTKENSKKLKMKFP
K396 TTKENSKKLKMKFPP
K398 KENSKKLKMKFPPKI
K416 KTKSKTNKGGITQPN
S427 TQPNINDSLEITKLD
T431 INDSLEITKLDSSII
K432 NDSLEITKLDSSIIS
S435 LEITKLDSSIISEGK
S436 EITKLDSSIISEGKI
S439 KLDSSIISEGKISTI
K442 SSIISEGKISTITPQ
T445 ISEGKISTITPQIQA
T447 EGKISTITPQIQAFN
S553 LQKDVALSVLSKDLT
K557 VALSVLSKDLTDMDK
S578 CAAGNCFSLQREHDI
K588 REHDIAIKFFQRAIQ
T691 KSEKALDTLNKAIVI
K694 KALDTLNKAIVIDPK
K718 SVLFANEKYKSALQE
K720 LFANEKYKSALQELE
K730 LQELEELKQIVPKES
K735 ELKQIVPKESLVYFL
Y740 VPKESLVYFLIGKVY
S821 TQLHAAESDEF____
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