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Protein Page:
ARNT (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
ARNT a bHLH transcription factor that requires dimerization with another bHLH protein for efficient DNA binding. A binding partner for the Ah (dioxin) nuclear receptor. Required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. A binding partner for HIF1A or HIF2A, transcription factors that regulates transcription from RNA polymerase II promoter in the adaptive response to hypoxia and oxidative stress. This complex activates target genes that limit oxygen consumption. HIF-1alpha may have nontranscriptional activities that inhibit DNA replication and cell proliferation when oxygen becomes scarce. Forms heterodimers with other bHLH proteins. Interacts with TACC3. Three isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Nuclear receptor; Transcription factor; DNA binding protein; Motility/polarity/chemotaxis
Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; aryl hydrocarbon receptor binding; protein heterodimerization activity; sequence-specific DNA binding; transcription coactivator activity; aryl hydrocarbon receptor activity; transcription factor activity; transcription factor binding
Biological Process: embryonic placenta development; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of erythrocyte differentiation; mRNA transcription from RNA polymerase II promoter; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of protein sumoylation; positive regulation of hormone biosynthetic process; positive regulation of glycolysis; response to hypoxia; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; cell differentiation
Reference #:  P27540 (UniProtKB)
Alt. Names/Synonyms: ARNT; ARNT protein; Aryl hydrocarbon receptor nuclear translocator; BHLHE2; Class E basic helix-loop-helix protein 2; Dioxin receptor, nuclear translocator; HIF-1-beta; HIF-1beta; HIF1-beta; HIF1B; HIF1BETA; hypoxia-inducible factor 1, beta subunit; Hypoxia-inducible factor 1-beta; TANGO
Gene Symbols: ARNT
Molecular weight: 86,636 Da
Basal Isoelectric point: 6.11  Predict pI for various phosphorylation states
CST Pathways:  Angiogenesis  |  mTOR Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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ARNT

Protein Structure Not Found.


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Sites Implicated In
transcription, inhibited: S77‑p
activity, inhibited: S77‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 T11-p TTANPEMtSDVPSLG
0 2 K58-ac DDGEGNSkFLRCDDD
0 1 S68-p RCDDDQMsNDKERFA
1 16 S77-p DKERFARsDDEQssA
0 1 S82-p ARsDDEQssADKERL
0 1 S83-p RsDDEQssADKERLA
1 0 K245 DLKTGTVKKEGQQSS
1 0 S348-p GRLQVTSsPNCtDMS
0 1 T352-p VTSsPNCtDMSNVCQ
0 10 S558-p QDRDPRFsEIyHNIN
0 112 Y561-p DPRFsEIyHNINADQ
0 1 T593-p QGNTFPPtPRPAENF
0 13 T636-p SNPTQGAtPtWtPTT
0 4 T638-p PTQGAtPtWtPTTRS
0 5 T640-p QGAtPtWtPTTRSGF
0 1 K659 VATQATAKTRTSQFG
0 1 S669-p TSQFGVGsFQTPSSF
0 1 S788-p LTMFPPFsE______
  mouse

 
T11 TTANPEMTSDVPSLG
K58 DEVEVNTKFLRCDDD
C68 RCDDDQMCNDKERFA
S77-p DKERFARsDDEQSSA
S82 ARsDDEQSSADKERL
S83 RsDDEQSSADKERLA
K245-sm DLKTGTVkKEGQQSS
S348 GRLQVTSSPNCTDMS
T352 VTSSPNCTDMSNICQ
P560 QDRDPRFPEIYPSIT
Y563 DPRFPEIYPSITADQ
N595 QGSSFPPNPRPAENF
A638 SNPAQGSAPTWTSSS
T640 PAQGSAPTWTSSSRP
T642 QGSAPTWTSSSRPGF
K661-ub VPTQATAkTRSSQFG
N671 SSQFGVNNFQTSSSF
S790 LTMFPPFSE______
  rat

 
T11 TTANPEMTSDVPSLG
K58 DEGEVNSKFLRCDDE
C68 RCDDEQMCNDKERFA
S77-p DKERFARsDDEQSSA
S82 ARsDDEQSSADKERL
S83 RsDDEQSSADKERLA
K245 DLKTGTVKKEGQQSS
S348 GRLQVTSSPNCTDMS
T352 VTSSPNCTDMSNICQ
S570 QDRDPRFSEIYPNIS
Y573 DPRFSEIYPNISADQ
N605 QGSSFPPNPRPAENF
A648 SNLTQGSAPTWTSST
T650 LTQGSAPTWTSSTRP
T652 QGSAPTWTSSTRPGF
K670 LPTQATAKTRSSQFG
N680 SSQFGVNNFQTSSSF
S799 LTMFPPFSE______
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