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Protein Page:
ARNT (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
ARNT a bHLH transcription factor that requires dimerization with another bHLH protein for efficient DNA binding. A binding partner for the Ah (dioxin) nuclear receptor. Required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. A binding partner for HIF1A or HIF2A, transcription factors that regulates transcription from RNA polymerase II promoter in the adaptive response to hypoxia and oxidative stress. This complex activates target genes that limit oxygen consumption. HIF-1alpha may have nontranscriptional activities that inhibit DNA replication and cell proliferation when oxygen becomes scarce. Forms heterodimers with other bHLH proteins. Interacts with TACC3. Three isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: DNA binding protein; Motility/polarity/chemotaxis; Transcription factor; Nuclear receptor
Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; aryl hydrocarbon receptor binding; sequence-specific DNA binding; protein heterodimerization activity; transcription coactivator activity; aryl hydrocarbon receptor activity; transcription factor binding; transcription factor activity
Biological Process: embryonic placenta development; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of erythrocyte differentiation; mRNA transcription from RNA polymerase II promoter; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of protein sumoylation; positive regulation of hormone biosynthetic process; positive regulation of glycolysis; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; cell differentiation
Reference #:  P27540 (UniProtKB)
Alt. Names/Synonyms: ARNT; ARNT protein; Aryl hydrocarbon receptor nuclear translocator; BHLHE2; Class E basic helix-loop-helix protein 2; Dioxin receptor, nuclear translocator; HIF-1-beta; HIF-1beta; HIF1-beta; HIF1B; HIF1BETA; hypoxia-inducible factor 1, beta subunit; Hypoxia-inducible factor 1-beta; TANGO
Gene Symbols: ARNT
Molecular weight: 86,636 Da
Basal Isoelectric point: 6.11  Predict pI for various phosphorylation states
CST Pathways:  Angiogenesis  |  mTOR Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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ARNT

Protein Structure Not Found.


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Sites Implicated In
transcription, inhibited: S77‑p
activity, inhibited: S77‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 2 K58-a DDGEGNSkFLRCDDD
0 1 S68-p RCDDDQMsNDKERFA
1 14 S77-p DKERFARsDDEQSSA
1 0 K245 DLKTGTVKKEGQQSS
1 0 S348-p GRLQVTSsPNCTDMS
0 9 S558-p QDRDPRFsEIyHNIN
0 126 Y561-p DPRFsEIyHNINADQ
0 13 T636-p SNPTQGAtPtWtPTT
0 4 T638-p PTQGAtPtWtPTTRS
0 5 T640-p QGAtPtWtPTTRSGF
  mouse

 
K58 DEVEVNTKFLRCDDD
C68 RCDDDQMCNDKERFA
S77-p DKERFARsDDEQSSA
K245-s DLKTGTVkKEGQQSS
S348 GRLQVTSSPNCTDMS
P560 QDRDPRFPEIYPSIT
Y563 DPRFPEIYPSITADQ
A638 SNPAQGSAPTWTSSS
T640 PAQGSAPTWTSSSRP
T642 QGSAPTWTSSSRPGF
  rat

 
K58 DEGEVNSKFLRCDDE
C68 RCDDEQMCNDKERFA
S77-p DKERFARsDDEQSSA
K245 DLKTGTVKKEGQQSS
S348 GRLQVTSSPNCTDMS
S570 QDRDPRFSEIYPNIS
Y573 DPRFSEIYPNISADQ
A648 SNLTQGSAPTWTSST
T650 LTQGSAPTWTSSTRP
T652 QGSAPTWTSSTRPGF
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