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iNOS (human)

Overview iNOS Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. Homodimer. Binds SLC9A3R1. By endotoxins and cytokines. Induced by IFNG/IFN-gamma acting synergistically with bacterial lipopolysaccharides (LPS), TNF or IL1B/interleukin-1 beta. Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity. Belongs to the NOS family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB. Protein type: Oxidoreductase; Amino Acid Metabolism - arginine and proline; EC 1.14.13.39 Cellular Component: cortical cytoskeleton; perinuclear region of cytoplasm; cytoplasm; peroxisome; intracellular; cytosol; nucleus Molecular Function: calmodulin binding; protein binding; protein homodimerization activity; FAD binding; FMN binding; nitric-oxide synthase activity; iron ion binding; heme binding; NADP binding; receptor binding Biological Process: interaction with host; superoxide metabolic process; positive regulation of killing of cells of another organism; defense response to Gram-negative bacterium; regulation of cell proliferation; regulation of cellular respiration; positive regulation of leukocyte mediated cytotoxicity; innate immune response in mucosa; response to bacterium; defense response to bacterium; response to hypoxia; peptidyl-cysteine S-nitrosylation; arginine catabolic process; negative regulation of protein catabolic process; nitric oxide biosynthetic process; blood coagulation; regulation of insulin secretion Reference #:  P35228 (UniProtKB) Alt. Names/Synonyms: HEP-NOS; Hepatocyte NOS; Inducible NO synthase; Inducible NOS; iNOS; nitric oxide synthase 2, inducible; nitric oxide synthase 2A (inducible, hepatocytes); Nitric oxide synthase, inducible; nitric oxide synthase, macrophage; NOS; NOS type II; NOS, type II; NOS2; NOS2A Gene Symbols: NOS2 Molecular weight: 131,117 Da Basal Isoelectric point: 8.2  Predict pI for various phosphorylation states CST Pathways:  Angiogenesis  |  Insulin Receptor Signaling Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below iNOS 1NSI - A/B/C/D=74-504 (human) 2LL6 - B=515-531 (human) 2NSI - A/B/C/D=74-504 (human) 3E7G - A/D/C/B=82-505 (human) 3EJ8 - A/D/C/B=82-505 (human) 3HR4 - A/C/G/E=503-715 (human) 4NOS - A/B/C/D=82-508 (human) 1DD7 - A=114-498 (mouse) 1DF1 - A/B=77-499 (mouse) 1DWV - A/B=77-496 (mouse) 1DWW - A/B=77-496 (mouse) 1DWX - A/B=77-496 (mouse) 1JWJ - A/B=66-498 (mouse) 1JWK - A/B=66-498 (mouse) 1M8D - A/B=65-498 (mouse) 1M8E - A/B=65-498 (mouse) 1M8H - A/B=65-498 (mouse) 1M8I - A/B=65-498 (mouse) 1M9T - A/B=65-498 (mouse) 1N2N - A/B=77-495 (mouse) 1NOC - A=115-498 (mouse) 1NOD - A/B=77-499 (mouse) 1NOS - A=115-498 (mouse) 1QOM - A/B=65-498 (mouse) 1QW4 - A/B=77-495 (mouse) 1QW5 - A/B=77-495 (mouse) 1R35 - A/B=66-498 (mouse) 1VAF - A/B=77-495 (mouse) 2BHJ - A=77-498 (mouse) 2NOD - A/B=77-499 (mouse) 2NOS - A=115-498 (mouse) 2ORO - A=114-498 (mouse) 2ORP - A=114-498 (mouse) 2ORQ - A=114-498 (mouse) 2ORR - A=114-498 (mouse) 2ORS - A=114-498 (mouse) 2ORT - A=114-498 (mouse) 2Y37 - A/B=66-498 (mouse) 3DWJ - A/B=66-496 (mouse) 3E65 - A/B=66-498 (mouse) 3E67 - A/B=66-498 (mouse) 3E68 - A/B=66-498 (mouse) 3E6L - A/B=66-498 (mouse) 3E6N - A/B=66-498 (mouse) 3E6O - A/B=66-498 (mouse) 3E6T - A/B=66-498 (mouse) 3E7I - A/B=66-498 (mouse) 3E7M - A/B=66-498 (mouse) 3E7T - A/B=66-498 (mouse) 3EAI - A/B=66-498 (mouse) 3EBD - A/B=66-498 (mouse) 3EBF - A/B=66-498 (mouse) 3GOF - D/C=503-518 (mouse) 3NOD - A/B=77-499 (mouse) 3NQS - A/B=66-498 (mouse) 3NW2 - A/B=77-499 (mouse)

STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB 1NSI 2LL6 2NSI 3E7G 3EJ8 3HR4 4NOS  |  ENZYME  |  Phospho3D  |  DISEASE 145500 163730 248310 611162  |  Source  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene

	Sites Implicated In
enzymatic activity, induced: S745‑p enzymatic activity, inhibited: Y151‑p intracellular localization: Y151‑p protein stabilization: Y1055‑p

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
0	1		S50-p	DLQYHNLsKQQNESP
0	1		T126-p	IMTPKSLtRGPRDKP
2	0		Y151-p	IEFVNQYyGSFKEAK
0	1		Y267-p	WNAQLIRyAGyQMPD
0	1		Y270-p	QLIRyAGyQMPDGSI
0	1		K428	AVLHSFQKQNVTIMD
0	1		Y446-p	AAESFMKyMQNEyRs
0	1		Y451-p	MKyMQNEyRsRGGCP
0	1		S453-p	yMQNEyRsRGGCPAD
0	1		K516-u	KRREIPLkVLVKAVL
0	1		P567	LFSCAFNPKVVCMDK
0	1		Y575-p	KVVCMDKyRLSCLEE
0	1		K604-a	DCPGNGEkLKKSLFM
0	1		D714	HHYRLVQDSQPLDLS
0	1		K722	SQPLDLSKALSSMHA
1	0		S739	VFTMRLKSRQNLQsP
1	1		S745-p	KSRQNLQsPTSSRAT
0	1		Y868-p	ALCQPSEySKWKFTN
0	2		S892-p	EFPSLRVsAGFLLsQ
0	2		S898-p	VsAGFLLsQLPILKP
1	0		S909	ILKPRFYSISSSRDH
0	1		R1047	AVHTAYSRLPGKPKV
1	5		Y1055-p	LPGKPKVyVQDILRQ
0	2		Y1123-p	QLKSQKRyHEDIFGA
0	2		Y1134-p	IFGAVFPyEAKKDRV
0	1		-	under review

	mouse
-	gap
T120	IMNPKSLTRGPRDKP
Y145	IEFINQYYGSFKEAK
Y261	WNSQLIRYAGYQMPD
Y264	QLIRYAGYQMPDGTI
K422-m1	AVLHSFQkQNVTIMD
H440	ASESFMKHMQNEYRA
Y445	MKHMQNEYRARGGCP
A447	HMQNEYRARGGCPAD
R510	RRREIRFRVLVKVVF
T561	LFSYAFNTKVVCMDQ
Y569	KVVCMDQYKASTLEE
T598	DCPSNGQTLKKSLFM
S708-p	QQYRLIQsPEPLDLN
R716-m1	PEPLDLNrALSSIHA
S733-p	VFTMRLKsQQNLQSE
S739	KsQQNLQSEKSSRTT
Y862	ALCQPSEYNDWKFSN
P886	EFPSLHVPAAFLLSQ
S892	VPAAFLLSQLPILKP
S903-p	ILKPRYYsISSSQDH
R1041-m1	QVHTGYSrLPGKPKV
Y1049-p	LPGKPKVyVQDILQK
Y1117	QLKSQKRYHEDIFGA
Y1128	IFGAVFSYGAKKGsA
S1134-p	SYGAKKGsALEEPKA

	rat
-	gap
T123	IMNSKSLTRGPRDKP
Y148	IEFINQYYGSFKEAK
Y264	WNSQLIRYAGYQMPD
Y267	QLIRYAGYQMPDGTI
K425	AVLHSFQKQNVTIMD
H443	ASESFMKHMQNEYRA
Y448	MKHMQNEYRARGGCP
A450	HMQNEYRARGGCPAD
T513	RRREIRFTVLVKAVF
T564-p	LFSYAFNtKVVCMEQ
Y572	KVVCMEQYKANTLEE
T601	DCPSNGQTLKKSLFM
S711	EQYKLTQSPESLDLN
K719	PESLDLNKALSSIHA
S736	VFTMRLKSLQNLQSE
S742	KSLQNLQSEKSSRTT
Y865	ALCQPSEYNDWKFSN
P889	EFPSLRVPAAFLLSQ
S895	VPAAFLLSQLPILKP
S906	ILKPRYYSISSSQDH
R1044	QVHTGYSRLPGKPKV
Y1052-p	LPGKPKVyVQDILQK
Y1120	QLKSQKRYHEDIFGA
Y1131	IFGAVFSYGAKKGNT
N1137	SYGAKKGNTLEEPKG

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