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Protein Page:
NKCC1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
NKCC1 a multi-pass membrane cation transporter protein. Aids transcellular movement of chloride across both secretory and absorptive epithelia. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume. Expressed in many tissues. Bumetanide is a potent NKCC1 antagonist. Two alternatively spliced isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Membrane protein, integral; Transporter; Membrane protein, multi-pass; Transporter, SLC family
Chromosomal Location of Human Ortholog: 5q23.3
Cellular Component: membrane; basolateral plasma membrane; integral to plasma membrane; apical plasma membrane; plasma membrane; vesicle
Molecular Function: protein binding; ammonium transmembrane transporter activity; sodium:potassium:chloride symporter activity
Biological Process: hyperosmotic response; transport; transepithelial chloride transport; multicellular organism growth; sodium ion transport; ion transport; detection of mechanical stimulus involved in sensory perception of sound; transmembrane transport; positive regulation of cell volume; gamma-aminobutyric acid signaling pathway; potassium ion transport; ammonium transport
Reference #:  P55011 (UniProtKB)
Alt. Names/Synonyms: Basolateral Na-K-Cl symporter; BSC; BSC2; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1; MGC104233; NKCC1; S12A2; SLC12A2; solute carrier family 12 (sodium/potassium/chloride transporters), member 2; Solute carrier family 12 member 2
Gene Symbols: SLC12A2
Molecular weight: 131,447 Da
Basal Isoelectric point: 5.98  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

NKCC1

Protein Structure Not Found.


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Sites Implicated In
activity, induced: T203‑p, T207‑p, T212‑p, T217‑p, T230‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 T984-p VEEEDGktATQPLLk
0 2 K991-ub tATQPLLkKEskGPI
2 17 S77-p RPLGPTPsQsRFQVD
0 8 S79-p LGPTPsQsRFQVDLV
0 1 K114-ub AGAGAGAkQTPADGE
0 1 K130-ub SGESEPAkGsEEAKG
0 2 S132-p ESEPAkGsEEAKGRF
7 0 T203-p HQHYYYDtHTNtyYL
8 0 T207-p YYDtHTNtyYLRtFG
0 6 Y208-p YDtHTNtyYLRtFGH
15 21 T212-p TNtyYLRtFGHNtMD
8 43 T217-p LRtFGHNtMDAVPRI
0 43 Y227-p AVPRIDHyRHtAAQL
3 6 T230-p RIDHyRHtAAQLGEK
1 4 S242-p GEKLLRPsLAELHDE
0 10 K252-ub ELHDELEkEPFEDGF
0 7 S265-p GFANGEEstPtRDAV
0 24 T266-p FANGEEstPtRDAVV
0 1 T268-p NGEEstPtRDAVVTy
0 91 Y275-p tRDAVVTytAESkGV
0 11 T276-p RDAVVTytAESkGVV
0 5 K280-ub VTytAESkGVVKFGW
0 2 Y354 VRGGGAYYLISRSLG
0 1 K631 KIFQALCKDNIYPAF
0 1 K833 GPRRQAMKEMSIDQA
0 1 K841 EMSIDQAKYQRWLIK
0 7 K848 KYQRWLIKNKMKAFy
0 1 Y855-p KNKMKAFyAPVHADD
0 1 K923-ub GVVVIRLkEGLDISH
0 7 S940-p GQEELLSsQEksPGt
0 2 K943-ub ELLSsQEksPGtkDV
0 2 S944-p LLSsQEksPGtkDVV
0 1 T947-p sQEksPGtkDVVVsV
0 2 K948-ub QEksPGtkDVVVsVE
0 1 S953-p GtkDVVVsVEyskkS
0 20 Y956-p DVVVsVEyskkSDLD
0 1 S957-p VVVsVEyskkSDLDT
0 1 K958-ub VVsVEyskkSDLDTS
0 1 K959-ub VsVEyskkSDLDTSk
0 1 K966-ub kSDLDTSkPLSEkPI
0 1 K971-ub TSkPLSEkPITHkVE
0 1 K976-ub SEkPITHkVEEEDGk
0 1 K983-ub kVEEEDGktATQPLL
0 9 S994-p QPLLkKEskGPIVPL
0 2 K995-ub PLLkKEskGPIVPLN
0 4 K1007-ub PLNVADQkLLEAsTQ
0 1 S1012-p DQkLLEAsTQFQkKQ
0 2 K1017-ub EAsTQFQkKQGKNTI
0 2 K1061-ub IRVFIGGkINRIDHD
0 1 K1078 AMATLLSKFRIDFSD
0 1 K1078 AMATLLSKFRIDFSD
0 2 K1095-ub VLGDINTkPKkENII
0 2 K1098-ub DINTkPKkENIIAFE
0 1 K1118-ub YRLHEDDkEQDIADk
0 1 K1125-ub kEQDIADkMKEDEPW
0 1 K1143 DNELELYKTKTYRQI
0 1 K1143-ub DNELELYkTKTYRQI
0 5 Y1211-p HQSVLTFys______
0 1 S1212-p QSVLTFys_______
  NKCC1 iso3  
- gap
- gap
S77 RPLGPTPSQSRFQVD
S79 LGPTPSQSRFQVDLV
K114 AGAGAGAKQTPADGE
K130 SGESEPAKGSEEAKG
S132 ESEPAKGSEEAKGRF
T203 HQHYYYDTHTNTYYL
T207 YYDTHTNTYYLRTFG
Y208 YDTHTNTYYLRTFGH
T212 TNTYYLRTFGHNTMD
T217 LRTFGHNTMDAVPRI
Y227 AVPRIDHYRHTAAQL
T230 RIDHYRHTAAQLGEK
S242 GEKLLRPSLAELHDE
K252 ELHDELEKEPFEDGF
S265 GFANGEESTPTRDAV
T266 FANGEESTPTRDAVV
T268 NGEESTPTRDAVVTY
Y275 TRDAVVTYTAESKGV
T276 RDAVVTYTAESKGVV
K280 VTYTAESKGVVKFGW
Y354 VRGGGAYYLISRSLG
K631 KIFQALCKDNIYPAF
K833 GPRRQAMKEMSIDQA
K841 EMSIDQAKYQRWLIK
K848 KYQRWLIKNKMKAFY
Y855 KNKMKAFYAPVHADD
K923 GVVVIRLKEGLDISH
S940 GQEELLSSQEKSPGT
K943 ELLSSQEKSPGTKDV
S944 LLSSQEKSPGTKDVV
T947 SQEKSPGTKDVVVSV
K948 QEKSPGTKDVVVSVE
S953 GTKDVVVSVEYSKKS
Y956 DVVVSVEYSKKSDLD
S957 VVVSVEYSKKSDLDT
K958 VVSVEYSKKSDLDTS
K959 VSVEYSKKSDLDTSK
K966 KSDLDTSKPLSEKPI
K971 TSKPLSEKPITHKES
- gap
- gap
S978 KPITHKESKGPIVPL
K979 PITHKESKGPIVPLN
K991 PLNVADQKLLEASTQ
S996 DQKLLEASTQFQKKQ
K1001 EASTQFQKKQGKNTI
K1045 IRVFIGGKINRIDHD
K1062 AMATLLSKFRIDFSD
K1062 AMATLLSKFRIDFSD
K1079 VLGDINTKPKKENII
K1082 DINTKPKKENIIAFE
K1102 YRLHEDDKEQDIADK
K1109 KEQDIADKMKEDEPW
K1127 DNELELYKTKTYRQI
K1127 DNELELYKTKTYRQI
Y1195 HQSVLTFYS______
S1196 QSVLTFYS_______
  mouse

 
T977 DEEEDGKTPTQPLLk
K984-ub TPTQPLLkKEskGPI
S74-p RPLGPTPsQsRFQVD
S76-p LGPTPsQsRFQVDPV
K109 AAAGAAGKETPAAGK
K125 GGESGVAKGSEEAKG
S127 ESGVAKGSEEAKGRF
T196-p HQQYYYDtHTNtYYL
T200-p YYDtHTNtYYLRtFG
Y201 YDtHTNtYYLRtFGH
T205-p TNtYYLRtFGHNtMD
T210-p LRtFGHNtMDAVPRI
Y220 AVPRIDHYRHtAAQL
T223-p RIDHYRHtAAQLGEK
S235 GEKLLRPSLAELHDE
K245-ub ELHDELEkEPFEDGF
S258-p GFANGEEstPTRDAV
T259-p FANGEEstPTRDAVV
T261 NGEEstPTRDAVVAy
Y268-p TRDAVVAyTAESkGV
T269 RDAVVAyTAESkGVV
K273-ub VAyTAESkGVVKFGW
Y347 VRGGGAYYLISRSLG
K624-ub KIFQALCkDNIYPAF
K826-ub GPRRQAMkEMSIDQA
R834 EMSIDQARYQRWLIk
K841-ub RYQRWLIkNKMKAFY
Y848 kNKMKAFYAPVHADD
K916 GVVVIRLKEGLDISH
S933-p GQEELLSsQEksPGT
K936-ub ELLSsQEksPGTkDV
S937-p LLSsQEksPGTkDVV
T940 sQEksPGTkDVVVNV
K941-ub QEksPGTkDVVVNVD
N946 GTkDVVVNVDYSKKS
Y949 DVVVNVDYSKKSDQD
S950 VVVNVDYSKKSDQDT
K951 VVNVDYSKKSDQDTC
K952 VNVDYSKKSDQDTCK
K959 KSDQDTCKSSGEKSI
K964 TCKSSGEKSITQKDE
K969 GEKSITQKDEEEDGK
K976 KDEEEDGKTPTQPLL
S987-p QPLLkKEskGPIVPL
K988-ub PLLkKEskGPIVPLN
K1000-ub PLNVADQkLLEAsTQ
S1005-p DQkLLEAsTQFQkKQ
K1010-ub EAsTQFQkKQGKNTI
K1054-ub IRVFIGGkINRIDHD
K1071 AMATLLSKFRIDFSD
K1071-ub AMATLLSkFRIDFSD
K1088-ub VLGDINTkPKkENII
K1091-ub DINTkPKkENIIAFD
K1111 YRLHEDDKEQDIADK
K1118 KEQDIADKMKEDEPW
K1136 DNELELYKTKTYRQI
K1136 DNELELYKTKTYRQI
Y1204 HQSVLTFYS______
S1205 QSVLTFYS_______
  rat

 
T975 DEEEDGKTSTQPLLK
K982 TSTQPLLKKEsKGPV
S73-p KPLGPTPsQSRFQVD
S75 LGPTPsQSRFQVDPV
K107 AAAGAAGKETPAAGK
K123 GTESGVAKGSEEAKG
S125 ESGVAKGSEEAKGRF
T194 HQQYYYDTHTNTYYL
T198 YYDTHTNTYYLRtFG
Y199 YDTHTNTYYLRtFGH
T203-p TNTYYLRtFGHNtMD
T208-p LRtFGHNtMDAVPRI
Y218 AVPRIDHYRHTAAQL
T221 RIDHYRHTAAQLGEK
S233 GEKLLRPSLGEFHDE
K243 EFHDELEKEPFEDGF
S256 GFANGEEStPTRDAV
T257-p FANGEEStPTRDAVV
T259 NGEEStPTRDAVVTY
Y266 TRDAVVTYTAESKGV
T267 RDAVVTYTAESKGVV
K271 VTYTAESKGVVKFGW
Y345-p VRGGGAYyLISRSLG
K622 KIFQALCKDNIYPAF
K824 GPRRQAMKEMSIDQA
K832-ac EMSIDQAkYQRWLIK
K839 kYQRWLIKNKMKAFY
Y846 KNKMKAFYAPVHADD
K914 GVVVIRLKEGLDISH
S931-p GQEELLSsQEKSPGT
K934 ELLSsQEKSPGTKDV
S935 LLSsQEKSPGTKDVV
T938 sQEKSPGTKDVVVNV
K939 QEKSPGTKDVVVNVD
N944 GTKDVVVNVDYSKKS
Y947 DVVVNVDYSKKSDQD
S948 VVVNVDYSKKSDQDA
K949 VVNVDYSKKSDQDAF
K950 VNVDYSKKSDQDAFK
K957 KSDQDAFKASGEKPI
K962 AFKASGEKPITQKDE
K967 GEKPITQKDEEEDGK
K974 KDEEEDGKTSTQPLL
S985-p QPLLKKEsKGPVAPL
K986 PLLKKEsKGPVAPLN
K998 PLNVADQKLLEASTQ
S1003 DQKLLEASTQFQKKQ
K1008 EASTQFQKKQGKNTI
K1052 IRVFIGGKINRIDHD
K1069-ac AMATLLSkFRIDFSD
K1069 AMATLLSKFRIDFSD
K1086 VLGDINTKPKKENIV
K1089 DINTKPKKENIVAFD
K1109 YRLHEDDKEQDIADK
K1116 KEQDIADKMKEDEPW
K1134-ac DNELELYkTKTYRQI
K1134 DNELELYKTKTYRQI
Y1202 HQSVLTFYS______
S1203 QSVLTFYS_______
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