Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Protein Page:
NKCC1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
NKCC1 a multi-pass membrane cation transporter protein. Aids transcellular movement of chloride across both secretory and absorptive epithelia. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume. Expressed in many tissues. Bumetanide is a potent NKCC1 antagonist. Two alternatively spliced isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Transporter; Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral
Cellular Component: membrane; basolateral plasma membrane; integral to plasma membrane; apical plasma membrane; plasma membrane
Molecular Function: protein binding; ammonium transmembrane transporter activity; sodium:potassium:chloride symporter activity
Biological Process: hyperosmotic response; transport; transepithelial chloride transport; multicellular organism growth; sodium ion transport; ion transport; detection of mechanical stimulus involved in sensory perception of sound; positive regulation of cell volume; transmembrane transport; gamma-aminobutyric acid signaling pathway; potassium ion transport; ammonium transport
Reference #:  P55011 (UniProtKB)
Alt. Names/Synonyms: Basolateral Na-K-Cl symporter; BSC; BSC2; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1; MGC104233; NKCC1; S12A2; SLC12A2; solute carrier family 12 (sodium/potassium/chloride transporters), member 2; Solute carrier family 12 member 2
Gene Symbols: SLC12A2
Molecular weight: 131,447 Da
Basal Isoelectric point: 5.98  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

NKCC1

Protein Structure Not Found.


STRING  |  Reactome  |  neXtProt  |  Protein Atlas  |  BioGPS  |  Scansite  |  Pfam  |  Phospho.ELM  |  NetworKIN  |  Source  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene


Sites Implicated In
activity, induced: T203‑p, T207‑p, T212‑p, T217‑p, T230‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
2 14 S77-p RPLGPTPsQsRFQVD
0 8 S79-p LGPTPsQsRFQVDLV
0 1 K114-ub AGAGAGAkQTPADGE
0 1 K130-ub SGESEPAkGsEEAKG
0 1 S132-p ESEPAkGsEEAKGRF
6 0 T203-p HQHYYYDtHTNtyYL
7 0 T207-p YYDtHTNtyYLRtFG
0 5 Y208-p YDtHTNtyYLRtFGH
14 19 T212-p TNtyYLRtFGHNtMD
8 40 T217-p LRtFGHNtMDAVPRI
0 43 Y227-p AVPRIDHyRHtAAQL
3 6 T230-p RIDHyRHtAAQLGEK
1 4 S242-p GEKLLRPsLAELHDE
0 10 K252-ub ELHDELEkEPFEDGF
0 7 S265-p GFANGEEstPtRDAV
0 23 T266-p FANGEEstPtRDAVV
0 1 T268-p NGEEstPtRDAVVTy
0 90 Y275-p tRDAVVTytAESkGV
0 11 T276-p RDAVVTytAESkGVV
0 5 K280-ub VTytAESkGVVKFGW
0 2 Y354 VRGGGAYYLISRSLG
0 1 K631 KIFQALCKDNIYPAF
0 1 K833 GPRRQAMKEMSIDQA
0 7 K848 KYQRWLIKNKMKAFy
0 1 Y855-p KNKMKAFyAPVHADD
0 1 K923-ub GVVVIRLkEGLDISH
0 4 S940-p GQEELLSsQEksPGt
0 2 K943-ub ELLSsQEksPGtkDV
0 2 S944-p LLSsQEksPGtkDVV
0 1 T947-p sQEksPGtkDVVVsV
0 2 K948-ub QEksPGtkDVVVsVE
0 1 S953-p GtkDVVVsVEyskkS
0 20 Y956-p DVVVsVEyskkSDLD
0 1 S957-p VVVsVEyskkSDLDT
0 1 K958-ub VVsVEyskkSDLDTS
0 1 K959-ub VsVEyskkSDLDTSk
0 1 K966-ub kSDLDTSkPLSEkPI
0 1 K971-ub TSkPLSEkPITHkVE
0 1 K976-ub SEkPITHkVEEEDGk
0 1 K983-ub kVEEEDGktATQPLL
0 1 T984-p VEEEDGktATQPLLk
0 2 K991-ub tATQPLLkKEskGPI
0 8 S994-p QPLLkKEskGPIVPL
0 2 K995-ub PLLkKEskGPIVPLN
0 4 K1007-ub PLNVADQkLLEAsTQ
0 1 S1012-p DQkLLEAsTQFQkKQ
0 2 K1017-ub EAsTQFQkKQGKNTI
0 2 K1061-ub IRVFIGGkINRIDHD
0 1 K1078 AMATLLSKFRIDFSD
0 2 K1095-ub VLGDINTkPKkENII
0 2 K1098-ub DINTkPKkENIIAFE
0 1 K1118-ub YRLHEDDkEQDIADk
0 1 K1125-ub kEQDIADkMKEDEPW
0 1 K1143-ub DNELELYkTKTYRQI
0 5 Y1211-p HQSVLTFys______
0 1 S1212-p QSVLTFys_______
  mouse

 
S74-p RPLGPTPsQsRFQVD
S76-p LGPTPsQsRFQVDPV
K109 AAAGAAGKETPAAGK
K125 GGESGVAKGSEEAKG
S127 ESGVAKGSEEAKGRF
T196-p HQQYYYDtHTNtYYL
T200-p YYDtHTNtYYLRtFG
Y201 YDtHTNtYYLRtFGH
T205-p TNtYYLRtFGHNtMD
T210-p LRtFGHNtMDAVPRI
Y220 AVPRIDHYRHtAAQL
T223-p RIDHYRHtAAQLGEK
S235 GEKLLRPSLAELHDE
K245-ub ELHDELEkEPFEDGF
S258-p GFANGEEstPTRDAV
T259-p FANGEEstPTRDAVV
T261 NGEEstPTRDAVVAy
Y268-p TRDAVVAyTAESkGV
T269 RDAVVAyTAESkGVV
K273-ub VAyTAESkGVVKFGW
Y347 VRGGGAYYLISRSLG
K624-ub KIFQALCkDNIYPAF
K826-ub GPRRQAMkEMSIDQA
K841-ub RYQRWLIkNKMKAFY
Y848 kNKMKAFYAPVHADD
K916 GVVVIRLKEGLDISH
S933 GQEELLSSQEksPGT
K936-ub ELLSSQEksPGTkDV
S937-p LLSSQEksPGTkDVV
T940 SQEksPGTkDVVVNV
K941-ub QEksPGTkDVVVNVD
N946 GTkDVVVNVDYSKKS
Y949 DVVVNVDYSKKSDQD
S950 VVVNVDYSKKSDQDT
K951 VVNVDYSKKSDQDTC
K952 VNVDYSKKSDQDTCK
K959 KSDQDTCKSSGEKSI
K964 TCKSSGEKSITQKDE
K969 GEKSITQKDEEEDGK
K976 KDEEEDGKTPTQPLL
T977 DEEEDGKTPTQPLLk
K984-ub TPTQPLLkKEskGPI
S987-p QPLLkKEskGPIVPL
K988-ub PLLkKEskGPIVPLN
K1000-ub PLNVADQkLLEAsTQ
S1005-p DQkLLEAsTQFQkKQ
K1010-ub EAsTQFQkKQGKNTI
K1054-ub IRVFIGGkINRIDHD
K1071-ub AMATLLSkFRIDFSD
K1088-ub VLGDINTkPKkENII
K1091-ub DINTkPKkENIIAFD
K1111 YRLHEDDKEQDIADK
K1118 KEQDIADKMKEDEPW
K1136 DNELELYKTKTYRQI
Y1204 HQSVLTFYS______
S1205 QSVLTFYS_______
  rat

 
S73 KPLGPTPSQSRFQVD
S75 LGPTPSQSRFQVDPV
K107 AAAGAAGKETPAAGK
K123 GTESGVAKGSEEAKG
S125 ESGVAKGSEEAKGRF
T194 HQQYYYDTHTNTYYL
T198 YYDTHTNTYYLRtFG
Y199 YDTHTNTYYLRtFGH
T203-p TNTYYLRtFGHNtMD
T208-p LRtFGHNtMDAVPRI
Y218 AVPRIDHYRHTAAQL
T221 RIDHYRHTAAQLGEK
S233 GEKLLRPSLGEFHDE
K243 EFHDELEKEPFEDGF
S256 GFANGEESTPTRDAV
T257 FANGEESTPTRDAVV
T259 NGEESTPTRDAVVTY
Y266 TRDAVVTYTAESKGV
T267 RDAVVTYTAESKGVV
K271 VTYTAESKGVVKFGW
Y345-p VRGGGAYyLISRSLG
K622 KIFQALCKDNIYPAF
K824 GPRRQAMKEMSIDQA
K839 KYQRWLIKNKMKAFY
Y846 KNKMKAFYAPVHADD
K914 GVVVIRLKEGLDISH
S931 GQEELLSSQEKSPGT
K934 ELLSSQEKSPGTKDV
S935 LLSSQEKSPGTKDVV
T938 SQEKSPGTKDVVVNV
K939 QEKSPGTKDVVVNVD
N944 GTKDVVVNVDYSKKS
Y947 DVVVNVDYSKKSDQD
S948 VVVNVDYSKKSDQDA
K949 VVNVDYSKKSDQDAF
K950 VNVDYSKKSDQDAFK
K957 KSDQDAFKASGEKPI
K962 AFKASGEKPITQKDE
K967 GEKPITQKDEEEDGK
K974 KDEEEDGKTSTQPLL
T975 DEEEDGKTSTQPLLK
K982 TSTQPLLKKEsKGPV
S985-p QPLLKKEsKGPVAPL
K986 PLLKKEsKGPVAPLN
K998 PLNVADQKLLEASTQ
S1003 DQKLLEASTQFQKKQ
K1008 EASTQFQKKQGKNTI
K1052 IRVFIGGKINRIDHD
K1069 AMATLLSKFRIDFSD
K1086 VLGDINTKPKKENIV
K1089 DINTKPKKENIVAFD
K1109 YRLHEDDKEQDIADK
K1116 KEQDIADKMKEDEPW
K1134 DNELELYKTKTYRQI
Y1202 HQSVLTFYS______
S1203 QSVLTFYS_______
Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.