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Protein Page:
HP1 alpha (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
HP1 alpha Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Interacts with SUV420H1 and SUV420H2. Interacts with HP1BP3. Interacts directly with ATRX, CHAF1A, LBR, NIPBL, SP100, STAM2 and TRIM28 via the chromoshadow domain. Can interact directly with CBX3 via the chromoshadow domain. Interacts with histone H3 methylated at 'Lys- 9'. Interacts with BAHD1, MIS12 and DSN1. Interacts with POGZ; POGZ and PXVXL motif-containing proteins such as INCENP and TRIM28 compete for interaction with CBX5. Interacts with INCENP and TRIM24. Interacts with JC virus agnoprotein; this interaction induces the dissociation of CBX5 from LBR, resulting in destabilization of the nuclear envelope. Interacts with CHAMP1. Interacts with ASXL1. Note: This description may include information from UniProtKB.
Protein type: Transcription, coactivator/corepressor
Cellular Component: kinetochore; nucleoplasm; histone methyltransferase complex; PML body; transcriptional repressor complex; histone deacetylase complex; nucleolus; nuclear envelope; nuclear heterochromatin; nucleus; chromocenter
Molecular Function: protein binding, bridging; protein binding; protein homodimerization activity; histone deacetylase binding; chromatin binding; methylated histone residue binding
Biological Process: viral reproduction; negative regulation of transcription, DNA-dependent; blood coagulation
Reference #:  P45973 (UniProtKB)
Alt. Names/Synonyms: Antigen p25; CBX5; chromobox homolog 5 (HP1 alpha homolog, Drosophila); Chromobox protein homolog 5; Heterochromatin protein 1 homolog alpha; heterochromatin protein 1-alpha; HP1; HP1 alpha; HP1-ALPHA; HP1A; HP1Hs alpha
Gene Symbols: CBX5
Molecular weight: 22,225 Da
Basal Isoelectric point: 5.71  Predict pI for various phosphorylation states
CST Pathways:  Crosstalk between PTMs  |  Histone Methylation
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

HP1 alpha

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 6 T8-p MGKKTKRtADssssE
1 20 S11-p KTKRtADssssEDEE
1 20 S12-p TKRtADssssEDEEE
1 26 S13-p KRtADssssEDEEEy
1 29 S14-p RtADssssEDEEEyV
0 3 Y20-p ssEDEEEyVVEKVLD
0 1 K32-m1 VLDRRVVkGQVEYLL
0 2 K32-ub VLDRRVVkGQVEYLL
0 1 K40-ac GQVEYLLkWKGFsEE
0 1 K40-m1 GQVEYLLkWKGFsEE
0 1 K40-ub GQVEYLLkWKGFsEE
0 1 S45-p LLkWKGFsEEHNTWE
0 1 K55-ac HNTWEPEkNLDCPEL
0 2 K55-ub HNTWEPEkNLDCPEL
0 1 K68 ELISEFMKKYKKMKE
0 1 K91-ac KSESNKRksNFsNsA
0 1 K91-m1 KSESNKRksNFsNsA
0 8 K91-ub KSESNKRksNFsNsA
1 43 S92-p SESNKRksNFsNsAD
1 51 N93 ESNKRksNFsNsADD
1 11 S95-p NKRksNFsNsADDIk
1 15 S97-p RksNFsNsADDIkSk
0 7 K102-ub sNsADDIkSkKkREQ
0 2 K104-ub sADDIkSkKkREQsN
0 1 K106-ac DDIkSkKkREQsNDI
0 4 S110-p SkKkREQsNDIARGF
0 23 K125-ub ERGLEPEkIIGAtDs
0 1 T130-p PEkIIGAtDsCGDLM
0 4 S132-p kIIGAtDsCGDLMFL
0 1 K143-ac LMFLMKWkDTDEADL
0 1 K143-m1 LMFLMKWkDTDEADL
0 2 K143-ub LMFLMKWkDTDEADL
0 1 K154-m2 EADLVLAkEANVkCP
0 41 K154-ub EADLVLAkEANVkCP
0 7 K159-ub LAkEANVkCPQIVIA
0 3 Y177-p ERLTWHAyPEDAENk
0 9 K184-ub yPEDAENkEKETAKS
  mouse

 
T8-p MGKKTKRtADssssE
S11-p KTKRtADssssEDEE
S12-p TKRtADssssEDEEE
S13-p KRtADssssEDEEEy
S14-p RtADssssEDEEEyV
Y20-p ssEDEEEyVVEKVLD
K32 VLDRRMVKGQVEYLL
K32-ub VLDRRMVkGQVEYLL
K40 GQVEYLLKWKGFSEE
K40 GQVEYLLKWKGFSEE
K40 GQVEYLLKWKGFSEE
S45 LLKWKGFSEEHNTWE
K55 HNTWEPEKNLDCPEL
K55 HNTWEPEKNLDCPEL
K68-ub ELISEFMkKYKKMKE
K91 KSEGNKRKssFsNsA
K91 KSEGNKRKssFsNsA
K91-ub KSEGNKRkssFsNsA
S92-p SEGNKRkssFsNsAD
S93-p EGNKRkssFsNsADD
S95-p NKRkssFsNsADDIk
S97-p RkssFsNsADDIkSK
K102-ub sNsADDIkSKKKREQ
K104 sADDIkSKKKREQSN
K106 DDIkSKKKREQSNDI
S110 SKKKREQSNDIARGF
K125-ub ERGLEPEkIIGATDS
T130 PEkIIGATDSCGDLM
S132 kIIGATDSCGDLMFL
K143 LMFLMKWKDTDEADL
K143 LMFLMKWKDTDEADL
K143-ub LMFLMKWkDTDEADL
K154 EADLVLAKEANVkCP
K154-ub EADLVLAkEANVkCP
K159-ub LAkEANVkCPQIVIA
Y177 ERLTWHAYPEDAENk
K184-ub YPEDAENkEKESAKS
  rat

 
T8 MGKKTKRTADSSSsE
S11 KTKRTADSSSsEDEE
S12 TKRTADSSSsEDEEE
S13 KRTADSSSsEDEEEY
S14-p RTADSSSsEDEEEYV
Y20 SsEDEEEYVVEKVLD
K32 VLDRRMVKGQVEYLL
K32 VLDRRMVKGQVEYLL
K40 GQVEYLLKWKGFSEE
K40 GQVEYLLKWKGFSEE
K40 GQVEYLLKWKGFSEE
S45 LLKWKGFSEEHNTWE
K55 HNTWEPEKNLDCPEL
K55 HNTWEPEKNLDCPEL
K68 ELISEFMKKYKKMKE
K91 KSEGNKRKssFSNsA
K91 KSEGNKRKssFSNsA
K91 KSEGNKRKssFSNsA
S92-p SEGNKRKssFSNsAD
S93-p EGNKRKssFSNsADD
S95 NKRKssFSNsADDIK
S97-p RKssFSNsADDIKSK
K102 SNsADDIKSKKKREQ
K104 sADDIKSKKKREQSN
K106 DDIKSKKKREQSNDI
S110 SKKKREQSNDIARGF
K125 ERGLEPEKIIGATDS
T130 PEKIIGATDSCGDLM
S132 KIIGATDSCGDLMFL
K143 LMFLMKWKDTDEADL
K143 LMFLMKWKDTDEADL
K143 LMFLMKWKDTDEADL
K154 EADLVLAKEANVKCP
K154 EADLVLAKEANVKCP
K159 LAKEANVKCPQIVIA
Y177 ERLTWHAYPEDAENK
K184 YPEDAENKEKESAKS
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