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Protein Page:
AMPKA1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
AMPKA1 a catalytic subunit of AMP-activated protein kinase (AMPK). Acts as an energy sensor, playing a key role in regulating cellular energy metabolism. A protein kinase of the CAMKL family whose activation is regulated by the balance between ADP/AMP/ATP, and intracellular Ca(2+) levels. Acts as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-ADP and -AMP rise in response to fuel limitation and/or hypoxia. Activates energy-producing pathways and inhibits energy-consuming processes. Restores ATP levels in cells by switching off anabolic and switching on catabolic pathways. Activated primarily by rising ADP levels and not, as previously thought, solely by AMP. AMPK resembles an adenylate charge regulatory system in which anabolic and catabolic pathways are regulated by adenine nucleotide ratios. Acts via direct phosphorylation of a number of metabolic enzymes and transcription regulators. Activated by at least two distinct upstream kinases: the tumor suppressor LKB1 and CaMKK2. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. AMPK is a heterotrimer of an alpha catalytic subunit (AMPKA1 or -2), a beta (AMPKB1 or -2) and a gamma non-catalytic subunit (AMPKG1, -2 or -3). Different possible combinations of subunits give rise to 12 different holoenzymes. Binding of ADP or AMP to non-catalytic gamma subunit (PRKAG1, -2 or -3) results in allosteric activation. AMPK is activated by antihyperglycemic drug metformin, a drug prescribed to patients with type 2 diabetes: in vivo, metformin seems to mainly inhibit liver gluconeogenesis. However, metformin can be used to activate AMPK in muscle and other cells in culture or ex vivo. Selectively inhibited by compound C (6-[4-(2-Piperidin-1-yl-ethoxy)-phenyl)]-3-pyridin-4-yl-pyyrazolo[1,5-a] pyrimidine. Activated by resveratrol, a natural polyphenol present in red wine, and S17834, a synthetic polyphenol. Two isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.11.31; EC 2.7.11.1; Protein kinase, CAMK; Kinase, protein; EC 2.7.11.27; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.26; Autophagy; CAMK group; CAMKL family; AMPK subfamily
Chromosomal Location of Human Ortholog: 5p12
Cellular Component: nucleoplasm; apical plasma membrane; cytoplasm; intracellular; nucleus; cytosol; AMP-activated protein kinase complex
Molecular Function: protein C-terminus binding; AMP-activated protein kinase activity; protein binding; cAMP-dependent protein kinase activity; metal ion binding; tau-protein kinase activity; [acetyl-CoA carboxylase] kinase activity; histone serine kinase activity; chromatin binding; kinase binding; ATP binding; protein kinase activity; [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity
Biological Process: protein heterooligomerization; activation of MAPK activity; lipid biosynthetic process; rhythmic process; positive regulation of cholesterol biosynthetic process; glucose homeostasis; signal transduction; protein amino acid phosphorylation; response to caffeine; cellular response to glucose starvation; cellular response to nutrient levels; regulation of transcription, DNA-dependent; negative regulation of lipid catabolic process; positive regulation of cell proliferation; response to gamma radiation; negative regulation of glucosylceramide biosynthetic process; cell cycle arrest; positive regulation of autophagy; fatty acid biosynthetic process; response to UV; negative regulation of TOR signaling pathway; Wnt receptor signaling pathway; transcription, DNA-dependent; glucose metabolic process; regulation of circadian rhythm; cholesterol biosynthetic process; cold acclimation; fatty acid homeostasis; positive regulation of glycolysis; insulin receptor signaling pathway; response to hypoxia; autophagy; fatty acid oxidation; response to activity; negative regulation of apoptosis
Reference #:  Q13131 (UniProtKB)
Alt. Names/Synonyms: 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; AAPK1; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK; AMPK alpha 1; AMPK subunit alpha-1; AMPK1; AMPKa1; MGC33776; MGC57364; PRKAA1; protein kinase, AMP-activated, alpha 1 catalytic subunit
Gene Symbols: PRKAA1
Molecular weight: 64,009 Da
Basal Isoelectric point: 8.32  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

AMPKA1

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
enzymatic activity, induced: T183‑p
intracellular localization: T183‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
1 0 T368 FLDDHHLTRPHPERV
9 63 S496-p AtPQRsGsVsNyRSC
0 3 S524-p SEVsLtssVtsLDSs
0 1 T526-p VsLtssVtsLDSsPV
0 1 S6-p __MRRLSsWRKMATA
0 3 T32-p GHYILGDtLGVGTFG
0 3 K80-ac RREIQNLkLFRHPHI
1 5 S172-p KIADFGLsNMMsDGE
0 3 S176-p FGLsNMMsDGEFLRt
185 40 T183-p sDGEFLRtsCGsPNY
1 19 S184-p DGEFLRtsCGsPNYA
0 16 S187-p FLRtsCGsPNYAAPE
0 1 Y247-p GIFYTPQyLNPSVIS
2 1 T269 VDPMKRATIKDIREH
0 1 K285-ub WFKQDLPkYLFPEDP
0 2 S293-p YLFPEDPsySSTMID
0 10 Y294-p LFPEDPsySSTMIDD
0 1 T355-p AKDFYLAtsPPDsFL
0 6 S356-p KDFYLAtsPPDsFLD
2 4 S360-p LAtsPPDsFLDDHHL
0 17 T382-p VPFLVAEtPRARHtL
1 2 T388-p EtPRARHtLDELNPQ
0 2 K396-ub LDELNPQksKHQGVR
1 1 S397-p DELNPQksKHQGVRK
0 2 Y441-p EWKVVNPyyLRVRRK
0 10 Y442-p WKVVNPyyLRVRRKN
0 2 Y463-p SKMSLQLyQVDsRTY
0 1 S467-p LQLyQVDsRTYLLDF
1 2 T482-p RSIDDEItEAksGtA
0 1 K485-ub DDEItEAksGtAtPQ
3 36 S486-p DEItEAksGtAtPQR
1 10 T488-p ItEAksGtAtPQRsG
1 70 T490-p EAksGtAtPQRsGsV
3 12 S494-p GtAtPQRsGsVsNyR
0 2 S498-p PQRsGsVsNyRSCQR
0 2 Y500-p RsGsVsNyRSCQRsD
1 0 S502 GsVsNyRSCQRsDsD
1 1 S506-p NyRSCQRsDsDAEAQ
1 22 S508-p RSCQRsDsDAEAQGK
0 1 S520-p QGKSSEVsLtssVts
0 1 T522-p KSSEVsLtssVtsLD
0 1 S523-p SSEVsLtssVtsLDS
0 4 S527-p sLtssVtsLDSsPVD
0 2 S531-p sVtsLDSsPVDLTPR
2537 : Phospho-AMPKalpha1 (Ser485) (45F5) Rabbit mAb
4184 : Phospho-AMPKalpha1 (Ser485) Antibody
4185 : Phospho-AMPKalpha1 (Ser485)/AMPKalpha2 (Ser491) Antibody
2531 : Phospho-AMPKalpha (Thr172) Antibody
2535 : Phospho-AMPK-alpha (Thr172) (40H9) Rabbit mAb
4188 : Phospho-AMPK alpha (Thr172) (D79.5E) Rabbit mAb
5256 : Phospho-AMPK alpha (Thr172) (40H9) Rabbit mAb (Biotinylated)
  AMPKA1 iso2  
T383 FLDDHHLTRPHPERV
S511 ATPQRSGSVSNYRSC
S539 SEVSLTSSVTSLDSS
T541 VSLTSSVTSLDSSPV
S6 __MRRLSSWRKMATA
T32 GHYILGDTLGVGTFG
K80 RREIQNLKLFRHPHI
S187 KIADFGLSNMMSDGE
S191 FGLSNMMSDGEFLRT
T198 SDGEFLRTSCGSPNY
S199 DGEFLRTSCGSPNYA
S202 FLRTSCGSPNYAAPE
Y262 GIFYTPQYLNPSVIS
T284 VDPMKRATIKDIREH
K300 WFKQDLPKYLFPEDP
S308 YLFPEDPSYSSTMID
Y309 LFPEDPSYSSTMIDD
T370 AKDFYLATSPPDSFL
S371 KDFYLATSPPDSFLD
S375 LATSPPDSFLDDHHL
T397 VPFLVAETPRARHTL
T403 ETPRARHTLDELNPQ
K411 LDELNPQKSKHQGVR
S412 DELNPQKSKHQGVRK
Y456 EWKVVNPYYLRVRRK
Y457 WKVVNPYYLRVRRKN
Y478 SKMSLQLYQVDSRTY
S482 LQLYQVDSRTYLLDF
T497 RSIDDEITEAKSGTA
K500 DDEITEAKSGTATPQ
S501 DEITEAKSGTATPQR
T503 ITEAKSGTATPQRSG
T505 EAKSGTATPQRSGSV
S509 GTATPQRSGSVSNYR
S513 PQRSGSVSNYRSCQR
Y515 RSGSVSNYRSCQRSD
S517 GSVSNYRSCQRSDSD
S521 NYRSCQRSDSDAEAQ
S523 RSCQRSDSDAEAQGK
S535 QGKSSEVSLTSSVTS
T537 KSSEVSLTSSVTSLD
S538 SSEVSLTSSVTSLDS
S542 SLTSSVTSLDSSPVD
S546 SVTSLDSSPVDLTPR
  mouse

 
T368 FLDDHHLTRPHPERV
S496-p AtPQRsGsIsNYRsC
S524 SDVSLTSSVTSLDSS
T526 VSLTSSVTSLDSSPV
S6 __MRRLSSWRKMATA
T32-p GHYILGDtLGVGTFG
K80-ac RREIQNLkLFRHPHI
S172-p KIADFGLsNMMsDGE
S176-p FGLsNMMsDGEFLRt
T183-p sDGEFLRtsCGsPNY
S184-p DGEFLRtsCGsPNYA
S187-p FLRtsCGsPNYAAPE
Y247 GIFYTPQYLNPSVIS
A269 VDPMKRAAIKDIREH
K285 WFKQDLPKYLFPEDP
S293 YLFPEDPSYSSTMID
Y294 LFPEDPSYSSTMIDD
T355-p AKDFYLAtsPPDsFL
S356-p KDFYLAtsPPDsFLD
S360-p LAtsPPDsFLDDHHL
T382-p VPFLVAEtPRARHTL
T388 EtPRARHTLDELNPQ
K396-ub LDELNPQkSKHQGVR
S397 DELNPQkSKHQGVRK
Y441 EWKVVNPYyLRVRRK
Y442-p WKVVNPYyLRVRRKN
Y463 SKMSLQLYQVDSRTY
S467 LQLYQVDSRTYLLDF
T482 RSIDDEITEAKsGtA
K485 DDEITEAKsGtAtPQ
S486-p DEITEAKsGtAtPQR
T488-p ITEAKsGtAtPQRsG
T490-p EAKsGtAtPQRsGsI
S494-p GtAtPQRsGsIsNYR
S498-p PQRsGsIsNYRsCQR
Y500 RsGsIsNYRsCQRsD
S502-p GsIsNYRsCQRsDsD
S506-p NYRsCQRsDsDAEAQ
S508-p RsCQRsDsDAEAQGK
S520 QGKPSDVSLTSSVTS
T522 KPSDVSLTSSVTSLD
S523 PSDVSLTSSVTSLDS
S527 SLTSSVTSLDSSPVD
S531 SVTSLDSSPVDVAPR
2537 : Phospho-AMPKalpha1 (Ser485) (45F5) Rabbit mAb
4184 : Phospho-AMPKalpha1 (Ser485) Antibody
4185 : Phospho-AMPKalpha1 (Ser485)/AMPKalpha2 (Ser491) Antibody
2531 : Phospho-AMPKalpha (Thr172) Antibody
2535 : Phospho-AMPK-alpha (Thr172) (40H9) Rabbit mAb
4188 : Phospho-AMPK alpha (Thr172) (D79.5E) Rabbit mAb
5256 : Phospho-AMPK alpha (Thr172) (40H9) Rabbit mAb (Biotinylated)
  rat

 
T368-p FLDDHHLtRPHPERV
S496-p AtPQRSGsISNYRSC
S524 SEVSLTSSVTSLDSS
T526 VSLTSSVTSLDSSPV
S6 __MRRLSSWRKMATA
T32 GHYILGDTLGVGTFG
K80-ac RREIQNLkLFRHPHI
S172-p KIADFGLsNMMSDGE
S176 FGLsNMMSDGEFLRt
T183-p SDGEFLRtsCGSPNY
S184-p DGEFLRtsCGSPNYA
S187 FLRtsCGSPNYAAPE
Y247 GIFYTPQYLNPSVIS
T269-p VDPMKRAtIKDIREH
K285 WFKQDLPKYLFPEDP
S293 YLFPEDPSYSSTMID
Y294 LFPEDPSYSSTMIDD
T355 AKDFYLATSPPDsFL
S356 KDFYLATSPPDsFLD
S360-p LATSPPDsFLDDHHL
T382 VPFLVAETPRARHTL
T388 ETPRARHTLDELNPQ
K396 LDELNPQKsKHQGVR
S397-p DELNPQKsKHQGVRK
Y441 EWKVVNPYYLRVRRK
Y442 WKVVNPYYLRVRRKN
Y463 SKMSLQLYQVDSRTY
S467 LQLYQVDSRTYLLDF
T482 RSIDDEITEAKsGtA
K485 DDEITEAKsGtAtPQ
S486-p DEITEAKsGtAtPQR
T488-p ITEAKsGtAtPQRSG
T490-p EAKsGtAtPQRSGsI
S494 GtAtPQRSGsISNYR
S498 PQRSGsISNYRSCQR
Y500 RSGsISNYRSCQRSD
S502 GsISNYRSCQRSDSD
S506 NYRSCQRSDSDAEAQ
S508 RSCQRSDSDAEAQGK
S520 QGKPSEVSLTSSVTS
T522 KPSEVSLTSSVTSLD
S523 PSEVSLTSSVTSLDS
S527 SLTSSVTSLDSSPVD
S531 SVTSLDSSPVDVAPR
2537 : Phospho-AMPKalpha1 (Ser485) (45F5) Rabbit mAb
4184 : Phospho-AMPKalpha1 (Ser485) Antibody
4185 : Phospho-AMPKalpha1 (Ser485)/AMPKalpha2 (Ser491) Antibody
2531 : Phospho-AMPKalpha (Thr172) Antibody
2535 : Phospho-AMPK-alpha (Thr172) (40H9) Rabbit mAb
4188 : Phospho-AMPK alpha (Thr172) (D79.5E) Rabbit mAb
5256 : Phospho-AMPK alpha (Thr172) (40H9) Rabbit mAb (Biotinylated)
  fruit fly

 
- gap
- gap
- gap
- gap
A6 __MPQMRAAAAEAVA
T33 GHYLLGATLGTGTFG
K81 RREIQNLKLFRHPHI
S173 KIADFGLSNMMLDGE
L177 FGLSNMMLDGEFLRT
T184 LDGEFLRTSCGSPNY
S185 DGEFLRTSCGSPNYA
S188 FLRTSCGSPNYAAPE
Y248 GIFPIPEYLNKQVVN
N270 VDPLKRANIEEIKKH
A286 WFQKDLPAYLFPSSI
E294 YLFPSSIEQDSNVID
Q295 LFPSSIEQDSNVIDT
G363 INNFFVAGSPPPPPP
S364 NNFFVAGSPPPPPPP
S376 PPPPVPQSSMDHQAP
- gap
- gap
K447 GGGAFPEKTARGGTP
T448 GGAFPEKTARGGTPI
Y494 EWKIINPYHVRVRRQ
H495 WKIINPYHVRVRRQN
Y516 SKMSLQLYQVDAKSY
A520 LQLYQVDAKSYLLDF
V535 KSLTNDEVEQGDDVI
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
2535 : Phospho-AMPK-alpha (Thr172) (40H9) Rabbit mAb
4188 : Phospho-AMPK alpha (Thr172) (D79.5E) Rabbit mAb
5256 : Phospho-AMPK alpha (Thr172) (40H9) Rabbit mAb (Biotinylated)
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