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Protein Page:
claspin (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
claspin required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks. Interacts with CHEK1 when phosphorylated. Interacts with ATR and RAD9A and these interactions are slightly reduced during checkpoint activation. Interacts with BRCA1 and this interaction increases during checkpoint activation. Note: This description may include information from UniProtKB.
Protein type: Cell cycle regulation; Actin binding protein
Cellular Component: nucleoplasm
Molecular Function: protein binding; DNA binding
Biological Process: peptidyl-serine phosphorylation; apoptosis; DNA damage checkpoint; activation of protein kinase activity; mitotic cell cycle DNA replication checkpoint; G2/M transition DNA damage checkpoint; DNA repair; DNA replication; cell structure disassembly during apoptosis
Reference #:  Q9HAW4 (UniProtKB)
Alt. Names/Synonyms: Claspin; claspin homolog (Xenopus laevis); CLSPN; hClaspin; MGC131612; MGC131613; MGC131615
Gene Symbols: CLSPN
Molecular weight: 151,094 Da
Basal Isoelectric point: 4.74  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

claspin

Protein Structure Not Found.


STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  Source  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene


Sites Implicated In
cell cycle regulation: S30‑p, S34‑p
chromatin organization, altered: T916‑p
molecular association, regulation: S30‑p, S34‑p, T916‑p, S945‑p
protein degradation: S30‑p, S34‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S26 ISQEEADSPSDsGQG
1 0 S30-p EADSPSDsGQGsYET
1 0 S34-p PSDsGQGsYETIGPL
0 2 S42 YETIGPLSEGDSDEE
0 2 S46 GPLSEGDSDEEIFVS
0 1 S53 SDEEIFVSKKLKNRk
1 0 K60-u SKKLKNRkVLQDsDs
0 7 S65-p NRkVLQDsDsETEDT
0 7 S67-p kVLQDsDsETEDTNA
0 1 Y81-p ASPEKTTyDsAEEEN
0 4 S83-p PEKTTyDsAEEENKE
0 1 A84 EKTTyDsAEEENKEN
0 1 Y93-p EENKENLyAGkNTKI
1 0 K96-u KENLyAGkNTKIKRI
0 1 S110 IYKTVADSDESYMEK
0 2 S113 TVADSDESYMEKSLY
0 1 Y120 SYMEKSLYQENLEAQ
0 7 S225-p GLEDENNsPLEDEES
0 1 S260-p SLESGVHsFEEGSEL
0 1 S282-p ERKAARLsKEALKQL
0 25 Y306-p ESALNLPyHMPENKT
0 5 L428 SPGDSSVLQQESNFL
0 2 S516-p RLGADEDsFVILEPE
0 1 K544 KHANPAAKPRAGQTV
0 1 S714-p GKAVGFLsVPKsLss
0 2 S718-p GFLsVPKsLssDstL
0 7 S720-p LsVPKsLssDstLLL
0 1 S721-p sVPKsLssDstLLLF
0 11 S723-p PKsLssDstLLLFKD
0 3 T724-p KsLssDstLLLFKDs
0 1 S731-p tLLLFKDsSSkMGYF
0 1 K734-u LFKDsSSkMGYFPTE
0 2 S744-p YFPTEEKsETDENSG
0 1 S762-p SKLDEDDsCSLLTKE
0 1 S764 LDEDDsCSLLTKESS
0 1 S775-p KESSHNSsFELIGST
0 1 T802 RGTSFFPTAGGFRsP
0 10 S808-p PTAGGFRsPsPGLFR
0 19 S810-p AGGFRsPsPGLFRAS
0 1 S833-p SGKLSEPsLPIEDsQ
0 7 S839-p PsLPIEDsQDLyNAs
0 1 Y843-p IEDsQDLyNAsPEPK
0 8 S846-p sQDLyNAsPEPKTLF
0 11 Y887-p VRNHRNQyQALkPRL
0 1 K891-a RNQyQALkPRLPLAS
3 0 T916-p DELLDLCtGKFTSQA
0 2 K925-a KFTSQAEkHLPRKSD
2 0 S945-p EELLNLCsGKFtsQD
0 3 T949-p NLCsGKFtsQDAstP
0 10 S950-p LCsGKFtsQDAstPA
0 9 S954-p KFtsQDAstPAsSEL
0 13 T955-p FtsQDAstPAsSELN
0 5 S958-p QDAstPAsSELNkQE
0 1 K963-u PAsSELNkQEKESSM
0 2 T987-p LCSGSFPtDKEEEDE
0 1 S1012-p SNDNEFDsDEDEHsD
0 1 S1018-p DsDEDEHsDsGNDLA
0 1 S1020-p DEDEHsDsGNDLALE
0 1 Y1117 DKRQLRLYQERYLAD
0 25 S1147-p WKNIDDAsQMDLFHR
0 28 S1156-p MDLFHRDsDDDQtEE
0 7 T1161-p RDsDDDQtEEQLDES
0 16 S1204-p EEEIGEDsQFMILAK
0 2 S1223-p KALQKNAsRPMVIQE
0 15 S1289-p NFVFHTLsPVKAEAA
0 1 V1291 VFHTLsPVKAEAAKE
0 1 S1299-p KAEAAKEsSKSQVKK
0 2 S1314-p RGPSFMTsPsPKHLK
0 3 S1316-p PSFMTsPsPKHLKTD
0 1 S1325 KHLKTDDSTSGLTRS
  mouse

 
S26-p LSQEAADsPVDSGQG
S30 AADsPVDSGQGSFET
S34 PVDSGQGSFETLEPL
S42-p FETLEPLsERDsDEE
S46-p EPLsERDsDEEIFVs
S53-p sDEEIFVsKKPKSRK
K60 sKKPKSRKVLQDsDs
S65-p SRKVLQDsDsEAEDR
S67-p KVLQDsDsEAEDRDD
Y81 DAPEKPTYDDsAEDT
D83 PEKPTYDDsAEDTQE
S84-p EKPTYDDsAEDTQEN
H93 EDTQENLHSGKSQSR
K96 QENLHSGKSQSRSFP
S109-p FPKALADsDEsDMEE
S112-p ALADsDEsDMEETPs
S119-p sDMEETPsQESPETQ
S220 GLEEENDSALEDEES
S255 TLESEAFSLEDGNEL
S277 ERKAARLSKEALKKL
Y301 ESALNLPYHMPESKT
S424-p SPGDRSMsQQESSIP
S522-p RLGADEDsFVILDEP
S551-p RHANPAAsPRACQTV
C692 IGKSVALCVPKPLSs
P696 VALCVPKPLSsDstL
S698 LCVPKPLSsDstLLL
S699-p CVPKPLSsDstLLLF
S701-p PKPLSsDstLLLFKD
T702-p KPLSsDstLLLFKDS
S709 tLLLFKDSSSKMGYF
K712 LFKDSSSKMGYFPTE
S722-p YFPTEEKsETDEYLA
S740 DKLDEDDSSsLLTKE
S742-p LDEDDSSsLLTKESS
S753-p KESSHNSsFELIGST
T780-p RGASFLPtAGFRsPs
S785-p LPtAGFRsPsPGLFR
S787-p tAGFRsPsPGLFRGS
S810 SGKLSEPSLPVEDsQ
S816-p PSLPVEDsQDLYTAs
Y820 VEDsQDLYTAsPEPK
S823-p sQDLYTAsPEPKTLF
Y864 IRNHRHRYQAVKPQL
K868 RHRYQAVKPQLPLAS
T893 DELLDLCTGQFTSQP
K903 FTSQPEEKCQPRKND
S923 EELLNLCSGKFPTQD
P927 NLCSGKFPTQDAsPV
T928 LCSGKFPTQDAsPVA
S932-p KFPTQDAsPVAPLGL
P933 FPTQDAsPVAPLGLR
P936 QDAsPVAPLGLRSQE
R940 PVAPLGLRSQEKESS
T965 LCSGSFPTDREEEGE
S990 SKENGFASDEDEHSD
S996 ASDEDEHSDSNDEEL
S998 DEDEHSDSNDEELAL
Y1094-p DKRRLRLyQERYLAD
S1124-p WKHIDDTsQMDLFHR
S1133-p MDLFHRDsDDDQVEE
V1138 RDsDDDQVEEQLDET
S1180-p EEDIGDDsQFMMLAK
S1199 KALQKNASHTVVVQE
S1265-p NFVFHTLsPtKAEAA
T1267-p VFHTLsPtKAEAAKD
S1275 KAEAAKDSSKPQVRR
S1290-p RGLSSMMsPsPKRLK
S1292-p LSSMMsPsPKRLKTN
S1301-p KRLKTNGsSPGPKRS
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