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Protein Page:
claspin (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
claspin required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks. Interacts with CHEK1 when phosphorylated. Interacts with ATR and RAD9A and these interactions are slightly reduced during checkpoint activation. Interacts with BRCA1 and this interaction increases during checkpoint activation. Note: This description may include information from UniProtKB.
Protein type: Cell cycle regulation; Actin binding protein
Cellular Component: nucleoplasm
Molecular Function: protein binding; DNA binding
Biological Process: peptidyl-serine phosphorylation; apoptosis; DNA damage checkpoint; activation of protein kinase activity; mitotic cell cycle DNA replication checkpoint; DNA repair; DNA replication; G2/M transition DNA damage checkpoint; cell structure disassembly during apoptosis
Reference #:  Q9HAW4 (UniProtKB)
Alt. Names/Synonyms: Claspin; claspin homolog (Xenopus laevis); CLSPN; hClaspin; MGC131612; MGC131613; MGC131615
Gene Symbols: CLSPN
Molecular weight: 151,094 Da
Basal Isoelectric point: 4.74  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

claspin

Protein Structure Not Found.


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Sites Implicated In
cell cycle regulation: S30‑p, S34‑p
chromatin organization, altered: T916‑p
molecular association, regulation: S30‑p, S34‑p, T916‑p, S945‑p
protein degradation: S30‑p, S34‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S26 ISQEEADSPSDsGQG
1 0 S30-p EADSPSDsGQGsYET
1 0 S34-p PSDsGQGsYETIGPL
0 2 S42 YETIGPLSEGDSDEE
0 2 S46 GPLSEGDSDEEIFVS
0 1 S53 SDEEIFVSKKLKNRk
1 0 K60-ub SKKLKNRkVLQDsDs
0 10 S65-p NRkVLQDsDsETEDT
0 10 S67-p kVLQDsDsETEDTNA
0 1 S75-p ETEDTNAsPEKTTyD
0 1 Y81-p AsPEKTTyDsAEEEN
0 6 S83-p PEKTTyDsAEEENKE
0 1 A84 EKTTyDsAEEENKEN
0 1 N88 yDsAEEENKENLyAG
0 1 Y93-p EENKENLyAGkNTKI
1 0 K96-ub KENLyAGkNTKIKRI
0 2 S110-p IYKTVADsDESYMEK
0 2 S113 TVADsDESYMEKSLy
0 2 Y120-p SYMEKSLyQENLEAQ
0 1 K187-ac MEKIRQLkkKETkNQ
0 1 K188-ac EKIRQLkkKETkNQE
0 1 K192-ac QLkkKETkNQEDDVE
0 1 S205-p VEQPFNDsGCLLVDK
0 9 S225-p GLEDENNsPLEDEES
0 1 S260-p SLESGVHsFEEGSEL
0 1 S282-p ERKAARLsKEALKQL
0 25 Y306-p ESALNLPyHMPENKT
0 1 K319-ub KTIHDFFkRKPRPTC
0 5 L428 SPGDSSVLQQESNFL
0 3 T493-p PEKVRRFtLDRLKQL
0 3 S516-p RLGADEDsFVILEPE
0 1 K544 KHANPAAKPRAGQTV
0 1 S703-p DKENNDGssEIGKAV
0 1 S704-p KENNDGssEIGKAVG
0 1 S714-p GKAVGFLsVPKsLss
0 2 S718-p GFLsVPKsLssDstL
0 7 S720-p LsVPKsLssDstLLL
0 1 S721-p sVPKsLssDstLLLF
0 13 S723-p PKsLssDstLLLFKD
0 4 T724-p KsLssDstLLLFKDs
0 1 S731-p tLLLFKDsSSkMGYF
0 1 K734-ub LFKDsSSkMGYFPTE
0 2 S744-p YFPTEEKsETDENSG
0 1 K756-ac NSGKQPSkLDEDDsC
0 2 S762-p SkLDEDDsCsLLTKE
0 2 S764-p LDEDDsCsLLTKESS
0 1 S774-p TKESSHNssFELIGS
0 1 S775-p KESSHNssFELIGST
0 1 T802 RGTSFFPTAGGFRsP
0 13 S808-p PTAGGFRsPsPGLFR
0 23 S810-p AGGFRsPsPGLFRAS
0 1 S833-p SGKLSEPsLPIEDsQ
0 9 S839-p PsLPIEDsQDLyNAs
0 2 Y843-p IEDsQDLyNAsPEPK
0 11 S846-p sQDLyNAsPEPKTLF
0 11 Y887-p VRNHRNQyQALkPRL
0 1 K891-ac RNQyQALkPRLPLAS
3 0 T916-p DELLDLCtGKFTSQA
0 2 K925-ac KFTSQAEkHLPRKSD
2 1 S945-p EELLNLCsGKFtsQD
0 3 T949-p NLCsGKFtsQDAstP
0 12 S950-p LCsGKFtsQDAstPA
0 11 S954-p KFtsQDAstPAssEL
0 15 T955-p FtsQDAstPAssELN
0 5 S958-p QDAstPAssELNkQE
0 1 S959-p DAstPAssELNkQEK
0 1 K963-ub PAssELNkQEKESSM
0 2 T987-p LCSGSFPtDKEEEDE
0 1 S1012-p SNDNEFDsDEDEHsD
0 1 S1018-p DsDEDEHsDsGNDLA
0 1 S1020-p DEDEHsDsGNDLALE
0 1 Y1117 DKRQLRLYQERYLAD
0 34 S1147-p WKNIDDAsQMDLFHR
0 37 S1156-p MDLFHRDsDDDQtEE
0 8 T1161-p RDsDDDQtEEQLDES
0 16 S1204-p EEEIGEDsQFMILAK
0 2 S1223-p KALQKNAsRPMVIQE
0 18 S1289-p NFVFHTLsPVKAEAA
0 2 V1291 VFHTLsPVKAEAAKE
0 1 S1299-p KAEAAKEsSKSQVKK
0 1 S1310-p QVKKRGPsFMTsPsP
0 4 S1314-p RGPsFMTsPsPKHLK
0 6 S1316-p PsFMTsPsPKHLKtD
0 1 T1322-p PsPKHLKtDDSTSGL
0 1 S1325 KHLKtDDSTSGLTRS
  mouse

 
S26-p LSQEAADsPVDSGQG
S30 AADsPVDSGQGSFET
S34 PVDSGQGSFETLEPL
S42-p FETLEPLsERDsDEE
S46-p EPLsERDsDEEIFVs
S53-p sDEEIFVsKKPKSRK
K60 sKKPKSRKVLQDsDs
S65-p SRKVLQDsDsEAEDR
S67-p KVLQDsDsEAEDRDD
A75 EAEDRDDAPEKPTYD
Y81 DAPEKPTYDDsAEDt
D83 PEKPTYDDsAEDtQE
S84-p EKPTYDDsAEDtQEN
T88-p YDDsAEDtQENLHSG
H93 EDtQENLHSGKSQSR
K96 QENLHSGKSQSRSFP
S109-p FPKALADsDEsDMEE
S112-p ALADsDEsDMEETPs
S119-p sDMEETPsQESPETQ
K182 MEKIRRLKKKETRCE
K183 EKIRRLKKKETRCEE
R187 RLKKKETRCEESDAD
S200 ADRPLNDSGCLLEDS
S220 GLEEENDSALEDEES
S255 TLESEAFSLEDGNEL
S277 ERKAARLSKEALKKL
Y301 ESALNLPYHMPESKT
K314 KTIHDFFKRKPRPTC
S424-p SPGDRSMsQQESSIP
T499 PEKVRRFTVDRLRQL
S522-p RLGADEDsFVILDEP
S551-p RHANPAAsPRACQTV
S682 DKENTDTSSDIGKSV
S683 KENTDTSSDIGKSVA
C692 IGKSVALCVPKPLSs
P696 VALCVPKPLSsDstL
S698 LCVPKPLSsDstLLL
S699-p CVPKPLSsDstLLLF
S701-p PKPLSsDstLLLFKD
T702-p KPLSsDstLLLFKDS
S709 tLLLFKDSSSKMGYF
K712 LFKDSSSKMGYFPTE
S722-p YFPTEEKsETDEYLA
K734 YLAKQSDKLDEDDSS
S740 DKLDEDDSSsLLTKE
S742-p LDEDDSSsLLTKESS
S752-p TKESSHNssFELIGS
S753-p KESSHNssFELIGST
T780-p RGASFLPtAGFRsPs
S785-p LPtAGFRsPsPGLFR
S787-p tAGFRsPsPGLFRGS
S810 SGKLSEPSLPVEDsQ
S816-p PSLPVEDsQDLYTAs
Y820 VEDsQDLYTAsPEPK
S823-p sQDLYTAsPEPKTLF
Y864 IRNHRHRYQAVKPQL
K868 RHRYQAVKPQLPLAS
T893 DELLDLCTGQFTSQP
K903 FTSQPEEKCQPRKND
S923 EELLNLCSGKFPTQD
P927 NLCSGKFPTQDAsPV
T928 LCSGKFPTQDAsPVA
S932-p KFPTQDAsPVAPLGL
P933 FPTQDAsPVAPLGLR
P936 QDAsPVAPLGLRSQE
L937 DAsPVAPLGLRSQEK
R940 PVAPLGLRSQEKESS
T965 LCSGSFPTDREEEGE
S990 SKENGFASDEDEHSD
S996 ASDEDEHSDSNDEEL
S998 DEDEHSDSNDEELAL
Y1094-p DKRRLRLyQERYLAD
S1124-p WKHIDDTsQMDLFHR
S1133-p MDLFHRDsDDDQVEE
V1138 RDsDDDQVEEQLDET
S1180-p EEDIGDDsQFMMLAK
S1199 KALQKNASHTVVVQE
S1265-p NFVFHTLsPtKAEAA
T1267-p VFHTLsPtKAEAAKD
S1275 KAEAAKDSSKPQVRR
S1286 QVRRRGLSSMMsPsP
S1290-p RGLSSMMsPsPKRLK
S1292-p LSSMMsPsPKRLKTN
T1298 PsPKRLKTNGsSPGP
S1301-p KRLKTNGsSPGPKRS
  rat

 
- gap
S26 LSQEEVDSGQGSFEA
S30 EVDSGQGSFEALELF
S38 FEALELFSERDSDEE
S42 ELFSERDSDEEIFVS
S49 SDEEIFVSKKPKSRK
K56 SKKPKSRKVLQDSDS
S61 SRKVLQDSDSEAEDN
S63 KVLQDSDSEAEDNAP
- under review  
Y75 NAPEKPTYDSAEENN
S77 PEKPTYDSAEENNEN
A78 EKPTYDSAEENNENL
N82 YDSAEENNENLHSGK
H86 EENNENLHSGKSQTI
K89 NENLHSGKSQTIKSI
S103 ISTALADSDESSMEG
S106 ALADSDESSMEGTPC
C113 SSMEGTPCQESLETQ
K176 REKIRRLKKKETRCE
K177 EKIRRLKKKETRCEE
R181 RLKKKETRCEESDAD
S194 ADRPLNDSGCLLEDS
S214 GLEEEDDSPLEDEES
S244 SLESETFSLEDESEL
S266 ERKAAKLSKEALKKL
Y290 ESALNLPYHMPESKT
K303 KTIHDFFKRKPRPSC
S412 SPGDRSVSQQESSIS
T476 PGKARRFTVDRLRQL
S499 RLGADEDSFVILDEP
S528 RHANPAASPRAHQTL
S677 DKENTDASSDVGKPV
S678 KENTDASSDVGKPVA
S687 VGKPVALSVPKPLSS
P691 VALSVPKPLSSDSTL
S693 LSVPKPLSSDSTLLL
S694 SVPKPLSSDSTLLLF
S696 PKPLSSDSTLLLFKD
T697 KPLSSDSTLLLFKDT
T704 TLLLFKDTSSKMGYF
K707 LFKDTSSKMGYFPTE
S717 YFPTEEKSETDEHMG
K729 HMGKQSDKLDEDDSS
S735 DKLDEDDSSSLLTKE
S737 LDEDDSSSLLTKESS
S747 TKESSHNSSFELIGS
S748 KESSHNSSFELIGST
T775 RGTSFLPTAGFRsPs
S780-p LPTAGFRsPsPGLFR
S782-p TAGFRsPsPGLFRGS
S805 SGKLSEPSLPIEDSQ
S811 PSLPIEDSQDLYTAS
Y815 IEDSQDLYTASPEPK
S818 SQDLYTASPEPKTLF
Y859 VRNHRNRYQALKPQL
K863 RNRYQALKPQLPLAS
T888 DELLDLCTGQFTSQS
K898 FTSQSEEKCQPRKND
S918 EELLNLCSGKFPSQD
P922 NLCSGKFPSQDASPV
S923 LCSGKFPSQDASPVA
S927 KFPSQDASPVAPLEL
P928 FPSQDASPVAPLELR
P931 QDASPVAPLELRTEE
- under review  
R935 PVAPLELRTEERESG
T960 LCSGSFPTDREEEGE
S985 SKENEFASDEGGHSD
S991 ASDEGGHSDSSDEEL
S993 DEGGHSDSSDEELAL
Y1089 DKRRLRLYQERYLAD
S1119 WKHIDDTSQMDLFHR
S1128 MDLFHRDSDDDQVEE
V1133 RDSDDDQVEEQLDET
S1175 EEDVGEDSQFMMLAK
S1194 KALQKNASRAVAVQE
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
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