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Protein Page:
UBE3A (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
UBE3A E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator p27Kip1. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo. Binds UBQLN1 and UBQLN2. Interacts with the 26S proteasome. Interacts with HCV core protein and targets it to degradation. Interacts with the E6 protein of the cancer- associated human papillomavirus types 16 and 18. The E6/E6-AP complex binds to and targets the p53/TP53 tumor-suppressor protein for ubiquitin-mediated proteolysis. Interacts with BPY2. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Ligase; Ubiquitin ligase; Ubiquitin conjugating system; Transcription, coactivator/corepressor; Nuclear receptor co-regulator; EC 6.3.2.19; EC 6.3.2.-
Cellular Component: proteasome complex; cytoplasm; nucleus; cytosol
Molecular Function: protein binding; transcription coactivator activity; ubiquitin-protein ligase activity; ligase activity
Biological Process: ubiquitin-dependent protein catabolic process; positive regulation of phosphoinositide 3-kinase cascade; protein autoubiquitination; viral reproduction; sperm entry; ovarian follicle development; androgen receptor signaling pathway; protein ubiquitination during ubiquitin-dependent protein catabolic process; positive regulation of transcription from RNA polymerase II promoter; brain development; proteolysis
Reference #:  Q05086 (UniProtKB)
Alt. Names/Synonyms: ANCR; AS; CTCL tumor antigen se37-2; E6-AP; E6AP; E6AP ubiquitin-protein ligase; EPVE6AP; FLJ26981; HPVE6A; human papilloma virus E6-associated protein; Human papillomavirus E6-associated protein; Oncogenic protein-associated protein E6-AP; Renal carcinoma antigen NY-REN-54; UBE3A; ubiquitin protein ligase E3A; Ubiquitin-protein ligase E3A
Gene Symbols: UBE3A
Molecular weight: 100,688 Da
Basal Isoelectric point: 5.12  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

UBE3A

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 - gap
0 2 K10 KLHQCYWKSGEPQSD
0 1 S22 QSDDIEASRMKRAAA
0 1 K25 DIEASRMKRAAAkHL
0 4 K30-ub RMKRAAAkHLIERYY
0 5 K71-ub DNNAAAIkALELYkI
0 13 K77-ub IkALELYkINAKLCD
0 2 K90-ub CDPHPSKkGASSAyL
0 14 Y96-p KkGASSAyLENskGA
0 1 S100-p SSAyLENskGAPNNS
0 1 K101 SAyLENsKGAPNNSC
0 5 K101-ub SAyLENskGAPNNSC
0 7 K123-ub KGARIDFkDVTyLTE
0 4 Y127-p IDFkDVTyLTEEkVY
0 11 K132-ub VTyLTEEkVYEILEL
0 1 S158-p RVIGRVFssAEALVQ
0 1 S159-p VIGRVFssAEALVQS
0 1 K184-ub ELKSLQAkDEDKDED
0 1 K195 KDEDEKEKAACsAAA
0 1 S199-p EKEKAACsAAAMEED
0 1 S210 MEEDSEASSSRIGDs
0 1 S211 EEDSEASSSRIGDss
0 1 S212 EDSEASSSRIGDssQ
0 8 S217-p SSSRIGDssQGDNNL
0 53 S218-p SSRIGDssQGDNNLQ
0 3 K226-ub QGDNNLQkLGPDDVS
0 1 R294-m1 FIIVMENrNLHSPEY
0 1 K311-ub MALPLFCkAMSKLPL
0 1 K315 LFCkAMSKLPLAAQG
0 4 K323-ub LPLAAQGkLIRLWSk
0 2 K330-ub kLIRLWSkYNADQIR
0 3 T342-p QIRRMMEtFQQLITy
0 2 Y349-p tFQQLITykVISNEF
0 13 K350-ub FQQLITykVISNEFN
0 5 K373-ub DAIVAASkCLKMVYY
0 6 K421-ub GEERRNKkGPRVDPL
0 1 K435 LETELGVKTLDCRKP
0 1 T465-p LEMDKDYtFFkVEtE
0 2 K468-ub DKDYtFFkVEtENKF
0 1 T471-p YtFFkVEtENKFSFM
0 1 T479-p ENKFSFMtCPFILNA
0 18 Y511-p ERRITVLysLVQGQQ
0 2 S512-p RRITVLysLVQGQQL
0 1 K552 MENPADLKkQLYVEF
0 2 K552-ub MENPADLkkQLYVEF
0 2 K553-ub ENPADLkkQLYVEFE
0 2 K711-ub PITNENRkEFVNLYS
0 5 Y720-p FVNLYSDyILNkSVE
0 2 K724-ub YSDyILNkSVEkQFK
0 1 K728-ub ILNkSVEkQFKAFRR
0 1 K802-ub HSFTDEQkRLFLQFT
0 1 K822 APVGGLGKLKMIIAk
0 1 K824 VGGLGKLKMIIAkNG
0 3 K829-ub KLKMIIAkNGPDTER
0 1 K864 KLKERLLKAITYAkG
0 2 K870-ub LKAITYAkGFGML__
  UBE3A iso3  
K6 __MATACKRSGEPQS
S8 MATACKRSGEPQSDD
S19-p QSDDIEAsRMKRAAA
K22 DIEAsRMKRAAAKHL
K27 RMKRAAAKHLIERYY
K68 DNNAAAIKALELYKI
K74 IKALELYKINAKLCD
K87 CDPHPSKKGASSAYL
Y93 KKGASSAYLENSKGA
S97 SSAYLENSKGAPNNS
K98 SAYLENSKGAPNNSC
K98 SAYLENSKGAPNNSC
K120 KGARIDFKDVTYLTE
Y124 IDFKDVTYLTEEKVY
K129 VTYLTEEKVYEILEL
S155 RVIGRVFSSAEALVQ
S156 VIGRVFSSAEALVQS
K181 ELKSLQAKDEDKDED
K192 KDEDEKEKAACSAAA
S196 EKEKAACSAAAMEED
S207 MEEDSEASSSRIGDS
S208 EEDSEASSSRIGDSS
S209 EDSEASSSRIGDSSQ
S214 SSSRIGDSSQGDNNL
S215 SSRIGDSSQGDNNLQ
K223 QGDNNLQKLGPDDVS
R291 FIIVMENRNLHSPEY
K308 MALPLFCKAMSKLPL
K312 LFCKAMSKLPLAAQG
K320 LPLAAQGKLIRLWSK
K327 KLIRLWSKYNADQIR
T339 QIRRMMETFQQLITY
Y346 TFQQLITYKVISNEF
K347 FQQLITYKVISNEFN
K370 DAIVAASKCLKMVYY
K418 GEERRNKKGPRVDPL
K432 LETELGVKTLDCRKP
T462 LEMDKDYTFFKVETE
K465 DKDYTFFKVETENKF
T468 YTFFKVETENKFSFM
T476 ENKFSFMTCPFILNA
Y508 ERRITVLYSLVQGQQ
S509 RRITVLYSLVQGQQL
K549 MENPADLKKQLYVEF
K549 MENPADLKKQLYVEF
K550 ENPADLKKQLYVEFE
K708 PITNENRKEFVNLYS
Y717 FVNLYSDYILNKSVE
K721 YSDYILNKSVEKQFK
K725 ILNKSVEKQFKAFRR
K799 HSFTDEQKRLFLQFT
K819 APVGGLGKLKMIIAK
K821 VGGLGKLKMIIAKNG
K826 KLKMIIAKNGPDTER
K861 KLKERLLKAITYAKG
K867 LKAITYAKGFGML__
  mouse

► Hide Isoforms
 
K22-ac HRMATACkSsPGESQ
S24-p MATACkSsPGESQSE
S36 QSEDIEASRMkRAAA
K39-ac DIEASRMkRAAAkHL
K44-ub RMkRAAAkHLIERYY
K85-ub DNNAAAIkALELYkI
K91-ub IkALELYkINAKLCD
K104-ub CDPHPSKkGASSAYL
Y110 KkGASSAYLENSkGA
S114 SSAYLENSkGASNNS
K115-ac SAYLENSkGASNNSE
K115-ub SAYLENSkGASNNSE
K134-ub KKEGKDFkDVIYLTE
Y138 KDFkDVIYLTEEKVY
K143 VIYLTEEKVYEIYEF
S169 RVIGRIFSSAEALVL
S170 VIGRIFSSAEALVLS
K195 ELKSLQEKDEDKDED
K206-ub KDEDEKEkAACSAAA
S210 EKEkAACSAAAMEED
S221-p MEEDSEAsssRMGDs
S222-p EEDSEAsssRMGDss
S223-p EDSEAsssRMGDssQ
S228-p sssRMGDssQGDNNV
S229-p ssRMGDssQGDNNVQ
K237 QGDNNVQKLGPDDVT
S305 FIILMENSNLHSPEY
K322 MALPLFCKAMCkLPL
K326-ub LFCKAMCkLPLEAQG
K334-ub LPLEAQGkLIRLWSK
K341 kLIRLWSKYTADQIR
T353-p QIRRMMEtFQQLITY
Y360 tFQQLITYkVISNEF
K361-ub FQQLITYkVISNEFN
N384 DAIVAASNCLKMVYY
K432-ub GDERRNKkGPRVDPL
K446-ub LETELGVkTLDCRKP
T476 LEMDKDYTFFkVETE
K479-ub DKDYTFFkVETENKG
T482 YTFFkVETENKGSFM
T490 ENKGSFMTCPFILNA
Y522 ERRITVLYSLVQGQQ
S523 RRITVLYSLVQGQQL
K563-ac MENPADLkkQLYVEF
K563-ub MENPADLkkQLYVEF
K564-ub ENPADLkkQLYVEFE
K721 PITNENRKEFVISYS
Y730 FVISYSDYILNKSVE
K734 YSDYILNKSVEKQFK
K738 ILNKSVEKQFKAFRR
K812 HSFTDEQKRLFLLFT
K832-ub APVGGLGkLkMIIAk
K834-ub VGGLGkLkMIIAkNG
K839-ub kLkMIIAkNGPDTER
K874-ub KLNVRLLkAITYAkG
K880-ub LkAITYAkGFGML__
  UBE3A iso3  
- gap
- gap
- gap
K2 ______MKRAAAKHL
K7 _MKRAAAKHLIERYY
K48 DNNAAAIKALELYKI
K54 IKALELYKINAKLCD
K67 CDPHPSKKGASSAYL
Y73 KKGASSAYLENSKGA
S77 SSAYLENSKGASNNS
K78 SAYLENSKGASNNSE
K78 SAYLENSKGASNNSE
K97 KKEGKDFKDVIYLTE
Y101 KDFKDVIYLTEEKVY
K106 VIYLTEEKVYEIYEF
S132 RVIGRIFSSAEALVL
S133 VIGRIFSSAEALVLS
K158 ELKSLQEKDEDKDED
K169 KDEDEKEKAACSAAA
S173 EKEKAACSAAAMEED
S184 MEEDSEASSSRMGDS
S185 EEDSEASSSRMGDSS
S186 EDSEASSSRMGDSSQ
S191 SSSRMGDSSQGDNNV
S192 SSRMGDSSQGDNNVQ
K200 QGDNNVQKLGPDDVT
S268 FIIVMENSNLHSPEY
K285 MALPLFCKAMCKLPL
K289 LFCKAMCKLPLEAQG
K297 LPLEAQGKLIRLWSk
K304-ub KLIRLWSkYSADQIR
T316 QIRRMMETFQQLITY
Y323 TFQQLITYKVISNEF
K324 FQQLITYKVISNEFN
K347-ub DAIVAASkCLKMVYY
K395 GDERRNKKGPRVDPL
K409 LETELGVKTLDCRKP
T439 LEMDKDYTFFKVETE
K442 DKDYTFFKVETENKF
T445 YTFFKVETENKFSFM
T453 ENKFSFMTCPFILNA
Y485 ERRITVLYSLVQGQQ
S486 RRITVLYSLVQGQQL
K526 MENPADLKKQLYVEF
K526 MENPADLKKQLYVEF
K527 ENPADLKKQLYVEFE
K685-ub PITNENRkEFVNLYS
Y694 FVNLYSDYILNkSVE
K698-ub YSDYILNkSVEKQFK
K702 ILNkSVEKQFKAFRR
K776 HSFTDEQKRLFLQFT
K796 APVGGLGKLKMIIAK
K798 VGGLGKLKMIIAKNG
K803 KLKMIIAKNGPDTER
K838 KLKERLLKAITYAKG
K844 LKAITYAKGFGML__
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