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Protein Page:
PKLR (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PKLR one of three rate-limiting enzymes in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule ATP and a pyruvate molecule, which is a central metabolic intermediate that can be used as a building block or oxidized further. There are several mammalian isozymes of pyruvate kinase encoded by different genes. The L type predominates in liver, and the M type in muscle and brain. Two alternatively spliced human isoforms have been described: the R- and L-types. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.1.40; Nucleotide Metabolism - purine; Kinase, other; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - glycolysis and gluconeogenesis
Cellular Component: cytosol
Molecular Function: potassium ion binding; magnesium ion binding; pyruvate kinase activity; ATP binding
Biological Process: pyruvate biosynthetic process; response to cAMP; glycolysis; response to lithium ion; positive regulation of cellular metabolic process; glucose metabolic process; response to other organism; endocrine pancreas development; response to ATP; cellular response to insulin stimulus; response to heat; ATP biosynthetic process; carbohydrate metabolic process; response to hypoxia; energy reserve metabolic process; response to glucose stimulus; phosphorylation; response to nutrient
Reference #:  P30613 (UniProtKB)
Alt. Names/Synonyms: KPYR; PK1; PKL; PKLR; PKR; PKRL; Pyruvate kinase 1; pyruvate kinase isozyme R/L; Pyruvate kinase isozymes R/L; pyruvate kinase type L; pyruvate kinase, liver and blood cell; pyruvate kinase, liver and RBC; R-type/L-type pyruvate kinase; Red cell/liver pyruvate kinase; RPK
Gene Symbols: PKLR
Molecular weight: 61,830 Da
Basal Isoelectric point: 7.65  Predict pI for various phosphorylation states
Select Structure to View Below

PKLR

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 S2-p ______MsIQENIsS
0 1 S8-p MsIQENIsSLQLRSW
0 1 S19-p LRSWVSKsQRDLAKs
0 1 S26-p sQRDLAKsILIGAPG
0 2 Y38-p APGGPAGyLRRAsVA
0 1 R40 GGPAGyLRRAsVAQL
0 1 R41 GPAGyLRRAsVAQLT
4 7 S43-p AGyLRRAsVAQLTQE
1 0 T48 RAsVAQLTQELGTAF
1 0 T53 QLTQELGTAFFQQQQ
0 1 K109 ERLKEMIKAGMNIAR
0 1 S144-p AVESFAGsPLSYRPV
0 7 K158-ub VAIALDTkGPEIRTG
0 1 A190 VLVTVDPAFRTRGNA
0 1 A190 VLVTVDPAFRTRGNA
0 1 A190 VLVTVDPAFRTRGNA
0 2 Y218-p VPVGGRIyIDDGLIS
0 1 K230 LISLVVQKIGPEGLV
0 1 K250 GGVLGSRKGVNLPGA
0 1 K290-m1 VFASFVRkAsDVAAV
0 3 S292-p ASFVRkAsDVAAVRA
0 1 K309 GPEGHGIKIISkIEN
0 1 K313-ub HGIKIISkIENHEGV
0 1 K321 IENHEGVKRFDEILE
0 33 K348-ac GIEIPAEkVFLAQkM
0 22 K348-ub GIEIPAEkVFLAQkM
0 19 K354-ub EkVFLAQkMMIGRCN
0 1 R382 ESMITKPRPTRAETS
0 1 K419 NFPVEAVKMQHAIAR
0 1 T556-p GDLVIVVtGWRPGsG
0 1 S562-p VtGWRPGsGyTNIMR
0 14 Y564-p GWRPGsGyTNIMRVL
  PKLR iso2  
- gap
- gap
- gap
- gap
Y7 _MEGPAGYLRRAsVA
R9 EGPAGYLRRAsVAQL
R10 GPAGYLRRAsVAQLT
S12-p AGYLRRAsVAQLTQE
T17 RAsVAQLTQELGTAF
T22 QLTQELGTAFFQQQQ
K78 ERLKEMIKAGMNIAR
S113 AVESFAGSPLSYRPV
K127 VAIALDTKGPEIRTG
A159 VLVTVDPAFRTRGNA
A159 VLVTVDPAFRTRGNA
A159 VLVTVDPAFRTRGNA
Y187 VPVGGRIYIDDGLIS
K199 LISLVVQKIGPEGLV
K219 GGVLGSRKGVNLPGA
K259 VFASFVRKASDVAAV
S261 ASFVRKASDVAAVRA
K278 GPEGHGIKIISKIEN
K282 HGIKIISKIENHEGV
K290 IENHEGVKRFDEILE
K317 GIEIPAEKVFLAQKM
K317 GIEIPAEKVFLAQKM
K323 EKVFLAQKMMIGRCN
R351 ESMITKPRPTRAETS
K388 NFPVEAVKMQHAIAR
T525 GDLVIVVTGWRPGSG
S531 VTGWRPGSGYTNIMR
Y533 GWRPGSGYTNIMRVL
  mouse

 
S2 ______MSVQENELP
L8 MSVQENELPQQLWPW
S19 LWPWIFKSQKDLAKS
S26 SQKDLAKSALSGAPG
Y38 APGGPAGYLrrAsVA
R40-m1 GGPAGYLrrAsVAQL
R41-m1 GPAGYLrrAsVAQLT
S43-p AGYLrrAsVAQLTQE
T48 rAsVAQLTQELGTAF
T53 QLTQELGTAFFQQQQ
K109-ac DRLKEMIkAGMNIAR
S144 AAESFATSPLSYRPV
K158-ub VAIALDTkGPEIRTG
K190-ac VLVTVDPkFRTRGDA
K190-ub VLVTVDPkFRTRGDA
K190-sc VLVTVDPkFRTRGDA
Y218 VAVGGRIYIDDGLIS
K230-ub LISLVVRkIGPEGLV
K250-ub GGFLGNRkGVNLPNA
K290 IFASFVRKAsDVVAV
S292-p ASFVRKAsDVVAVRD
K309 GPEGRGIKIISKIEN
K313 RGIKIISKIENHEGV
K321-sc IENHEGVkKFDEILE
K348-ac GIEIPAEkVFLAQkM
K348-ub GIEIPAEkVFLAQkM
K354-ub EkVFLAQkMMIGRCN
R382-m1 ESMITKArPTRAETS
K419-ub SFPVEAVkMQHAIAR
T556 GDLVIVVTGWRPGSG
S562 VTGWRPGSGYTNIMR
Y564 GWRPGSGYTNIMRVL
  rat

► Hide Isoforms
 
S2 ______MSVQENTLP
L8 MSVQENTLPQQLWPW
S19 LWPWIFRSQKDLAKS
S26 SQKDLAKSALSGAPG
Y38 APGGPAGYLRRASVA
R40 GGPAGYLRRASVAQL
R41 GPAGYLRRASVAQLt
S43 AGYLRRASVAQLtQE
T48-p RASVAQLtQELGtAF
T53-p QLtQELGtAFFQQQQ
K109 DRLKEMIKAGMNIAR
S144 ATESFATSPLSYRPV
K158 VAIALDTKGPEIRTG
K190 VLVTVDPKFQTRGDA
K190 VLVTVDPKFQTRGDA
K190 VLVTVDPKFQTRGDA
Y218 VAVGGRIYIDDGLIS
K230 LISLVVQKIGPEGLV
K250 GGILGSRKGVNLPNT
K290 IFASFVRKAsDVLAV
S292-p ASFVRKAsDVLAVRD
K309-ub GPEGQNIkIISKIEN
K313 QNIkIISKIENHEGV
K321 IENHEGVKKFDEILE
K348-ac GIEIPAEkVFLAQKM
K348 GIEIPAEKVFLAQKM
K354 EkVFLAQKMMIGRCN
R382 ESMITKARPTRAETS
M419 SFPVEAVMMQHAIAR
T556 GDLVIVVTGWRPGSG
S562 VTGWRPGSGYTNIMR
Y564 GWRPGSGYTNIMRVL
  PKLR iso2  
- gap
- gap
- gap
- gap
Y7 _MEGPAGYLRRAsVA
R9 EGPAGYLRRAsVAQL
R10 GPAGYLRRAsVAQLt
S12-p AGYLRRAsVAQLtQE
T17-p RAsVAQLtQELGtAF
T22-p QLtQELGtAFFQQQQ
K78 DRLKEMIKAGMNIAR
S113 ATESFATSPLSYRPV
K127 VAIALDTKGPEIRTG
K159 VLVTVDPKFQTRGDA
K159 VLVTVDPKFQTRGDA
K159 VLVTVDPKFQTRGDA
Y187 VAVGGRIYIDDGLIS
K199 LISLVVQKIGPEGLV
K219 GGILGSRKGVNLPNT
K259 IFASFVRKASDVLAV
S261 ASFVRKASDVLAVRD
K278-ub GPEGQNIkIISKIEN
K282 QNIkIISKIENHEGV
K290 IENHEGVKKFDEILE
K317-ac GIEIPAEkVFLAQKM
K317 GIEIPAEKVFLAQKM
K323 EkVFLAQKMMIGRCN
R351 ESMITKARPTRAETS
M388 SFPVEAVMMQHAIAR
T525 GDLVIVVTGWRPGSG
S531 VTGWRPGSGYTNIMR
Y533 GWRPGSGYTNIMRVL
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