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Protein Page:
PKLR (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
PKLR one of three rate-limiting enzymes in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule ATP and a pyruvate molecule, which is a central metabolic intermediate that can be used as a building block or oxidized further. There are several mammalian isozymes of pyruvate kinase encoded by different genes. The L type predominates in liver, and the M type in muscle and brain. Two alternatively spliced human isoforms have been described: the R- and L-types. Note: This description may include information from UniProtKB.
Protein type: Kinase, other; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 2.7.1.40; Nucleotide Metabolism - purine; Carbohydrate Metabolism - pyruvate
Cellular Component: cytosol
Molecular Function: potassium ion binding; magnesium ion binding; pyruvate kinase activity; ATP binding
Biological Process: pyruvate biosynthetic process; response to cAMP; glycolysis; response to lithium ion; positive regulation of cellular metabolic process; glucose metabolic process; response to other organism; endocrine pancreas development; response to ATP; cellular response to insulin stimulus; response to heat; ATP biosynthetic process; carbohydrate metabolic process; energy reserve metabolic process; response to hypoxia; response to glucose stimulus; response to nutrient
Reference #:  P30613 (UniProtKB)
Alt. Names/Synonyms: KPYR; PK1; PKL; PKLR; PKR; PKRL; Pyruvate kinase 1; pyruvate kinase isozyme R/L; Pyruvate kinase isozymes R/L; pyruvate kinase type L; pyruvate kinase, liver and blood cell; pyruvate kinase, liver and RBC; R-type/L-type pyruvate kinase; Red cell/liver pyruvate kinase; RPK
Gene Symbols: PKLR
Molecular weight: 61,830 Da
Basal Isoelectric point: 7.65  Predict pI for various phosphorylation states
Select Structure to View Below

PKLR

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 S2-p ______MsIQENIsS
0 1 S8-p MsIQENIsSLQLRSW
0 1 S19-p LRSWVSKsQRDLAKs
0 1 S26-p sQRDLAKsILIGAPG
0 2 Y38-p APGGPAGyLRRAsVA
4 6 S43-p AGyLRRAsVAQLTQE
1 0 T48 RAsVAQLTQELGTAF
1 0 T53 QLTQELGTAFFQQQQ
0 1 S144-p AVESFAGsPLSYRPV
0 7 K158-u VAIALDTkGPEIRTG
0 1 A190 VLVTVDPAFRTRGNA
0 2 Y218-p VPVGGRIyIDDGLIS
0 1 K230 LISLVVQKIGPEGLV
0 1 K250 GGVLGSRKGVNLPGA
0 2 S292-p ASFVRKAsDVAAVRA
0 32 K348-a GIEIPAEkVFLAQkM
0 22 K348-u GIEIPAEkVFLAQkM
0 19 K354-u EkVFLAQkMMIGRCN
0 1 K419 NFPVEAVKMQHAIAR
0 1 T556-p GDLVIVVtGWRPGSG
0 14 Y564-p GWRPGSGyTNIMRVL
  PKLR iso2  
- gap
- gap
- gap
- gap
Y7 _MEGPAGYLRRAsVA
S12-p AGYLRRAsVAQLTQE
T17 RAsVAQLTQELGTAF
T22 QLTQELGTAFFQQQQ
S113 AVESFAGSPLSYRPV
K127 VAIALDTKGPEIRTG
A159 VLVTVDPAFRTRGNA
Y187 VPVGGRIYIDDGLIS
K199 LISLVVQKIGPEGLV
K219 GGVLGSRKGVNLPGA
S261 ASFVRKASDVAAVRA
K317 GIEIPAEKVFLAQKM
K317 GIEIPAEKVFLAQKM
K323 EKVFLAQKMMIGRCN
K388 NFPVEAVKMQHAIAR
T525 GDLVIVVTGWRPGSG
Y533 GWRPGSGYTNIMRVL
  mouse

 
S2 ______MSVQENELP
L8 MSVQENELPQQLWPW
S19 LWPWIFKSQKDLAKS
S26 SQKDLAKSALSGAPG
Y38 APGGPAGYLRRAsVA
S43-p AGYLRRAsVAQLTQE
T48 RAsVAQLTQELGTAF
T53 QLTQELGTAFFQQQQ
S144 AAESFATSPLSYRPV
K158-u VAIALDTkGPEIRTG
K190-u VLVTVDPkFRTRGDA
Y218 VAVGGRIYIDDGLIS
K230-u LISLVVRkIGPEGLV
K250-u GGFLGNRkGVNLPNA
S292-p ASFVRKAsDVVAVRD
K348-a GIEIPAEkVFLAQkM
K348-u GIEIPAEkVFLAQkM
K354-u EkVFLAQkMMIGRCN
K419-u SFPVEAVkMQHAIAR
T556 GDLVIVVTGWRPGSG
Y564 GWRPGSGYTNIMRVL
  rat

► Hide Isoforms
 
S2 ______MSVQENTLP
L8 MSVQENTLPQQLWPW
S19 LWPWIFRSQKDLAKS
S26 SQKDLAKSALSGAPG
Y38 APGGPAGYLRRASVA
S43 AGYLRRASVAQLtQE
T48-p RASVAQLtQELGtAF
T53-p QLtQELGtAFFQQQQ
S144 ATESFATSPLSYRPV
K158 VAIALDTKGPEIRTG
K190 VLVTVDPKFQTRGDA
Y218 VAVGGRIYIDDGLIS
K230 LISLVVQKIGPEGLV
K250 GGILGSRKGVNLPNT
S292-p ASFVRKAsDVLAVRD
K348-a GIEIPAEkVFLAQKM
K348 GIEIPAEKVFLAQKM
K354 EkVFLAQKMMIGRCN
M419 SFPVEAVMMQHAIAR
T556 GDLVIVVTGWRPGSG
Y564 GWRPGSGYTNIMRVL
  PKLR iso2  
- gap
- gap
- gap
- gap
Y7 _MEGPAGYLRRAsVA
S12-p AGYLRRAsVAQLtQE
T17-p RAsVAQLtQELGtAF
T22-p QLtQELGtAFFQQQQ
S113 ATESFATSPLSYRPV
K127 VAIALDTKGPEIRTG
K159 VLVTVDPKFQTRGDA
Y187 VAVGGRIYIDDGLIS
K199 LISLVVQKIGPEGLV
K219 GGILGSRKGVNLPNT
S261 ASFVRKASDVLAVRD
K317-a GIEIPAEkVFLAQKM
K317 GIEIPAEKVFLAQKM
K323 EkVFLAQKMMIGRCN
M388 SFPVEAVMMQHAIAR
T525 GDLVIVVTGWRPGSG
Y533 GWRPGSGYTNIMRVL
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