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Protein Page:
TCF12 iso1 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
g O-GlcNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
TCF12 iso1 Binds specifically to oligomers of E-box motifs. May play important roles during development of the nervous system as well as in other organ systems. Efficient DNA binding requires dimerization with another bHLH protein. Forms homo- or hetero-oligomers with myogenin, E12 and ITF2 proteins. Interacts with PTF1A. Note: This description may include information from UniProtKB.
Protein type: DNA binding protein; Transcription factor
Cellular Component: transcription factor complex; nucleus
Molecular Function: protein binding; protein heterodimerization activity; transcription factor activity
Biological Process: regulation of transcription from RNA polymerase II promoter; muscle development; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; immune response; positive regulation of neuron differentiation
Reference #:  Q99081 (UniProtKB)
Alt. Names/Synonyms: BHLHB20; Class B basic helix-loop-helix protein 20; DNA-binding protein HTF4; E-box-binding protein; HEB; helix-loop-helix transcription factor 4; HsT17266; HTF4; TCF-12; TCF12; Transcription factor 12; Transcription factor HTF-4
Gene Symbols: TCF12
Molecular weight: 72,965 Da
Basal Isoelectric point: 6.52  Predict pI for various phosphorylation states
CST Pathways:  Adherens Junction Dynamics  |  Wnt/ß-Catenin Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

TCF12 iso1
Protein Structure Not Found.


Scansite  |  Pfam  |  RCSB PDB  |  Phospho3D  |  DISEASE  |  Source  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene


Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


  MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


        human

► Hide Isoforms 		 
0 1 T13-p QRMAAIGtDKELSDL
0 1 S43-p TRPTTLGssQFsGsG
0 9 S44-p RPTTLGssQFsGsGI
0 1 S47-p TLGssQFsGsGIDER
0 2 S49-p GssQFsGsGIDERGG
0 2 S59-p DERGGTTsWGTSGQP
0 6 S67-p WGTSGQPsPSYDSSR
0 1 Y70 SGQPsPSYDSSRGFT
0 1 S73 PsPSYDSSRGFTDsP
0 3 S79-p SSRGFTDsPHySDHL
0 13 Y82-p GFTDsPHySDHLNDS
0 1 S83 FTDsPHySDHLNDSR
0 5 S98-p LGAHEGLsPtPFMNS
0 1 T100-p AHEGLsPtPFMNSNL
0 8 Y120-p ERGSFSLySRDTGLP
0 2 T124 FSLySRDTGLPGCQS
0 1 S142-p RQDLGLGsPAQLSSS
0 3 Y156-p SGKPGTAyySFSATS
0 2 Y157-p GKPGTAyySFSATSS
0 2 S198-p PSSVYAPsPNsDDFN
0 1 S201-p VYAPsPNsDDFNRES
0 1 S263-p STSHMSQsSSYGNLH
0 1 S264 TSHMSQsSSYGNLHS
0 1 S281 RLSYPPHSVSPtDIN
0 1 T285-p PPHSVSPtDINTSLP
0 1 S301-p MSSFHRGstsssPyV
0 2 T302-p SSFHRGstsssPyVA
0 1 S303-p SFHRGstsssPyVAA
0 1 S304-p FHRGstsssPyVAAs
0 13 S305-p HRGstsssPyVAAsH
0 6 Y307-p GstsssPyVAAsHtP
0 8 S311-p ssPyVAAsHtPPING
0 34 T313-p PyVAAsHtPPINGSD
0 4 S321-p PPINGSDsILGTRGN
0 17 S333-p RGNAAGSsQtGDALG
0 2 T335-p NAAGSsQtGDALGKA
0 15 S385-p RPGGQAPssPsYENS
0 8 S386-p PGGQAPssPsYENSL
0 9 S388-p GQAPssPsYENSLHs
0 4 S392 ssPsYENSLHsLKNR
0 1 S395-p sYENSLHsLKNRVEQ
0 2 S486-p SLVASSRsAsMVGTH
0 6 S488-p VASSRsAsMVGTHRE
0 2 S497-p VGTHREDsVSLNGNH
0 1 S514-p LSSTVTTsSTDLNHK
0 5 S532-p NYRGGLQsQSGTVVT
0 1 S564 PSSDDMKSDDESsQK
0 7 S569-p MKSDDESsQKDIKVs
0 2 S576-p sQKDIKVssRGRtss
0 2 S577-p QKDIKVssRGRtsst
0 118 T581-p KVssRGRtsstNEDE
0 112 S582-p VssRGRtsstNEDED
0 280 S583-p ssRGRtsstNEDEDL
0 72 T584-p sRGRtsstNEDEDLN
0 1 K622 RDINEAFKELGRMCQ
0 1 K622-u RDINEAFkELGRMCQ
0 1 S634-p MCQLHLKsEKPQTKL
0 3 K666-u RERNLNPkAACLKRR
  TCF12 iso1  
T13 QRMAAIGTDKELSDL
S43 TRPTTLGSSQFSGSG
S44 RPTTLGSSQFSGSGI
S47 TLGSSQFSGSGIDER
S49 GSSQFSGSGIDERGG
S59 DERGGTTSWGTSGQP
S67 WGTSGQPSPSYDSSR
Y70 SGQPSPSYDSSRGFT
S73 PSPSYDSSRGFTDSP
S79 SSRGFTDSPHYSDHL
Y82 GFTDSPHYSDHLNDS
S83 FTDSPHYSDHLNDSR
S98 LGAHEGLSPTPFMNS
T100 AHEGLSPTPFMNSNL
Y120 ERGSFSLYSRDTGLP
T124 FSLYSRDTGLPGCQS
S142 RQDLGLGSPAQLSSS
Y156 SGKPGTAYYSFSATS
Y157 GKPGTAYYSFSATSS
S198 PSSVYAPSPNSDDFN
S201 VYAPSPNSDDFNRES
S263 STSHMSQSSSYGNLH
S264 TSHMSQSSSYGNLHS
S281 RLSYPPHSVSPTDIN
T285 PPHSVSPTDINTSLP
S301 MSSFHRGSTSSSPYV
T302 SSFHRGSTSSSPYVA
S303 SFHRGSTSSSPYVAA
S304 FHRGSTSSSPYVAAS
S305 HRGSTSSSPYVAASH
Y307 GSTSSSPYVAASHTP
S311 SSPYVAASHTPPING
T313 PYVAASHTPPINGSD
S321 PPINGSDSILGTRGN
S333 RGNAAGSSQTGDALG
T335 NAAGSSQTGDALGKA
S385-p RPGGQAPssPsYENs
S386-p PGGQAPssPsYENsL
S388-p GQAPssPsYENsLHS
S392-p ssPsYENsLHSLQSR
S395 sYENsLHSLQSRMED
S462 SLVASSRSASMVGTH
S464 VASSRSASMVGTHRE
S473 VGTHREDSVSLNGNH
S490 LSSTVTTSSTDLNHK
S508 NYRGGLQSQSGTVVT
S540 PSSDDMKSDDESSQK
S545 MKSDDESSQKDIKVS
S552 SQKDIKVSSRGRtsS
S553 QKDIKVSSRGRtsST
T557-p KVSSRGRtsSTNEDE
S558-p VSSRGRtsSTNEDED
S559 SSRGRtsSTNEDEDL
T560 SRGRtsSTNEDEDLN
K598 RDINEAFKELGRMCQ
K598 RDINEAFKELGRMCQ
S610 MCQLHLKSEKPQTKL
K642 RERNLNPKAACLKRR
  mouse

► Hide Isoforms 		 
T13 QRMAAIGTDKELSDL
S43 TRPTTLGSSQFSGSG
S44 RPTTLGSSQFSGSGM
S47 TLGSSQFSGSGMDER
S49 GSSQFSGSGMDERGG
S59 DERGGTTSWGTSGQP
S67-p WGTSGQPsPSYDSSR
Y70 SGQPsPSYDSSRGFT
S73 PsPSYDSSRGFTDsP
S79-p SSRGFTDsPHysDHL
Y82-p GFTDsPHysDHLNDS
S83-p FTDsPHysDHLNDSR
S98-p LGTHEGLsPTPFMNS
T100 THEGLsPTPFMNSNL
Y120 ERGSFSLYSRDsGLS
S124-p FSLYSRDsGLSGCQS
S142 RQDLGLGSPAQLSSS
Y156 SGKPGTPYYSFSATS
Y157 GKPGTPYYSFSATSS
S198-p PSSVYAPsPNSDDFN
S201 VYAPsPNSDDFNRES
S263 STSHMSQSsSYGSLH
S264-p TSHMSQSsSYGSLHS
S281-p RLSYPPHsVSPtDIN
T285-p PPHsVSPtDINTSLP
S301 MSSFHRGSTSSsPYV
T302 SSFHRGSTSSsPYVA
S303 SFHRGSTSSsPYVAA
S304 FHRGSTSSsPYVAAS
S305-p HRGSTSSsPYVAASH
Y307 GSTSSsPYVAASHTP
S311 SsPYVAASHTPPING
T313 PYVAASHTPPINGSD
S321 PPINGSDSILGTRGN
S333 RGNAAGSSQTGDALG
T335 NAAGSSQTGDALGKA
S385 RAGGQAPSSPSYENS
S386 AGGQAPSSPSYENSL
S388 GQAPSSPSYENSLHS
S392 SSPSYENSLHSLKNR
S395 SYENSLHSLKNRVEQ
S486 SLVTNSRSASMVGTH
S488 VTNSRSASMVGTHRE
S497 VGTHREDSVSLNGNH
S514 LSSTVAASNTELNHK
N532 NFRGGVQNQSGSVVP
S564-p PSSDDMKsDDESsQK
S569-p MKsDDESsQKDIKVS
S576 sQKDIKVSSRGRtss
S577 QKDIKVSSRGRtsst
T581-p KVSSRGRtsstNEDE
S582-p VSSRGRtsstNEDED
S583-p SSRGRtsstNEDEDL
T584-p SRGRtsstNEDEDLN
K622-a RDINEAFkELGRMCQ
K622 RDINEAFKELGRMCQ
S634 MCQLHLKSEKPQTKL
K666 RERNLNPKAACLKRR
  TCF12 iso2  
T13 QRMAAIGTDKELSDL
S43 TRPTTLGSSQFSGSG
S44 RPTTLGSSQFSGSGM
S47 TLGSSQFSGSGMDER
S49 GSSQFSGSGMDERGG
S59 DERGGTTSWGTSGQP
S67 WGTSGQPSPSYDSSR
Y70 SGQPSPSYDSSRGFT
S73 PSPSYDSSRGFTDSP
S79 SSRGFTDSPHYSDHL
Y82 GFTDSPHYSDHLNDS
S83 FTDSPHYSDHLNDSR
S98 LGTHEGLSPTPFMNS
T100 THEGLSPTPFMNSNL
Y120 ERGSFSLYSRDSGLS
S124 FSLYSRDSGLSGCQS
S142 RQDLGLGSPAQLSSS
Y156 SGKPGTPYYSFSATS
Y157 GKPGTPYYSFSATSS
S198 PSSVYAPSPNSDDFN
S201 VYAPSPNSDDFNRES
S263 STSHMSQSSSYGSLH
S264 TSHMSQSSSYGSLHS
S281 RLSYPPHSVSPTDIN
T285 PPHSVSPTDINTSLP
S301 MSSFHRGSTSSSPYV
T302 SSFHRGSTSSSPYVA
S303 SFHRGSTSSSPYVAA
S304 FHRGSTSSSPYVAAS
S305 HRGSTSSSPYVAASH
Y307 GSTSSSPYVAASHTP
S311 SSPYVAASHTPPING
T313 PYVAASHTPPINGSD
S321 PPINGSDSILGTRGN
S333 RGNAAGSSQTGDALG
T335 NAAGSSQTGDALGKA
S385-p RAGGQAPssPSYENs
S386-p AGGQAPssPSYENsL
S388 GQAPssPSYENsLHS
S392-p ssPSYENsLHSLQSR
S395 SYENsLHSLQSRMED
S462 SLVTNSRSASMVGTH
S464 VTNSRSASMVGTHRE
S473 VGTHREDSVSLNGNH
S490 LSSTVAASNTELNHK
N508 NFRGGVQNQSGSVVP
S540 PSSDDMKSDDESSQK
S545 MKSDDESSQKDIKVS
S552 SQKDIKVSSRGRTSS
S553 QKDIKVSSRGRTSST
T557 KVSSRGRTSSTNEDE
S558 VSSRGRTSSTNEDED
S559 SSRGRTSSTNEDEDL
T560 SRGRTSSTNEDEDLN
K598 RDINEAFKELGRMCQ
K598 RDINEAFKELGRMCQ
S610 MCQLHLKSEKPQTKL
K642 RERNLNPKAACLKRR
  rat

 
T13 QRMAAIGTDKELSDL
S43 TRPTTLGSSQFSGSG
S44 RPTTLGSSQFSGSGM
S47 TLGSSQFSGSGMDER
S49 GSSQFSGSGMDERGG
S59-p DERGGTTsWGTSGQP
S67 WGTSGQPSPSyDSsR
Y70-p SGQPSPSyDSsRGFT
S73-p PSPSyDSsRGFTDSP
S79 SsRGFTDSPHYSDHL
Y82 GFTDSPHYSDHLNDS
S83 FTDSPHYSDHLNDSR
S98 LGTHEGLSPTPFMNS
T100 THEGLSPTPFMNSNL
Y120 ERGSFSLYSRDSGLS
S124 FSLYSRDSGLSGCQS
S142 RQDLGLGSPAQLSSS
Y156 SGKPGTPYYSFSATS
Y157 GKPGTPYYSFSATSS
S198 PSSVYAPSPNSDDFN
S201 VYAPSPNSDDFNRES
S263 STSHMSQSSSYGSLH
S264 TSHMSQSSSYGSLHS
S281 RLSYPPHSVSPTDIN
T285 PPHSVSPTDINTSLP
S301 MSSFHRGSTSSSPYV
T302 SSFHRGSTSSSPYVA
S303 SFHRGSTSSSPYVAA
S304 FHRGSTSSSPYVAAS
S305 HRGSTSSSPYVAASH
Y307 GSTSSSPYVAASHTP
S311 SSPYVAASHTPPING
T313 PYVAASHTPPINGSD
S321 PPINGSDSILGTRGN
S333 RGNAAGSSQTGDALG
T335 NAAGSSQTGDALGKA
S386 RAGGQAPSSPSYENS
S387 AGGQAPSSPSYENSL
S389 GQAPSSPSYENSLHS
S393 SSPSYENSLHSLKNR
S396 SYENSLHSLKNRVEQ
S487 SLVTNSRSASMVGTH
S489 VTNSRSASMVGTHRE
S498 VGTHREDSVSLNGNH
S515 LSSTVAASNTELNHK
N533 SFRGGVQNQSGSVVP
S565 PSSDDMKSDDESSQK
S570 MKSDDESSQKDIKVS
S577 SQKDIKVSSRGRTSS
S578 QKDIKVSSRGRTSST
T582 KVSSRGRTSSTNEDE
S583 VSSRGRTSSTNEDED
S584 SSRGRTSSTNEDEDL
T585 SRGRTSSTNEDEDLN
K623 RDINEAFKELGRMCQ
K623 RDINEAFKELGRMCQ
S635 MCQLHLKSEKPQTKL
K667 RERNLNPKAACLKRR
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