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Protein Page:
E2A (mouse)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
E2A a transcription factor that plays major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Heterodimers between E2A and tissue-specific basic helix-loop-helix (bHLH) Dimers bind DNA on E-box motifs: 5'- CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Deletions in E2A have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Two alternatively spliced human isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Transcription factor; DNA binding protein
Cellular Component: transcription factor complex; protein complex; cytoplasm; nucleus
Molecular Function: protein dimerization activity; protein binding; protein homodimerization activity; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; transcription coactivator activity; bHLH transcription factor binding; chromatin binding; mitogen-activated protein kinase kinase kinase binding; transcription factor activity; transcription factor binding; PDZ domain binding
Biological Process: Peyer's patch development; response to drug; nervous system development; transcription, DNA-dependent; protein stabilization; immunoglobulin V(D)J recombination; B cell lineage commitment; positive regulation of transcription, DNA-dependent; T cell differentiation in the thymus; positive regulation of cell cycle; response to lipopolysaccharide; regulation of transcription from RNA polymerase II promoter; lymphocyte differentiation; regulation of transcription, DNA-dependent; natural killer cell differentiation; positive regulation of B cell proliferation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; cell differentiation; positive regulation of neuron differentiation; cell development
Reference #:  P15806 (UniProtKB)
Alt. Names/Synonyms: A1; AA408400; Alf2; AW209082; bHLHb21; E12; E12/E47; E2A; E47; Immunoglobulin enhancer-binding factor E12/E47; Me2; OTTMUSP00000020636; Pan1; Pan2; TCF-3; Tcf3; Tcfe2a; TFE2; Transcription factor 3; Transcription factor A1; Transcription factor E2-alpha; transcription factor E2a; VDIR
Gene Symbols: Tcf3
Molecular weight: 67,701 Da
Basal Isoelectric point: 5.94  Predict pI for various phosphorylation states
CST Pathways:  Adherens Junction Dynamics  |  Wnt/ß-Catenin Signaling
Select Structure to View Below

E2A

Protein Structure Not Found.


STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  Source  |  UCSD-Nature  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene  |  Ensembl Protein


Sites Implicated In
cell differentiation, altered: S140‑p
transcription, altered: S140‑p
molecular association, regulation: S140‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       mouse

► Hide Isoforms
 
1 0 K35 PLPVANGKSRPASLG
0 1 S40 NGKSRPASLGGTQFA
0 6 Y109 KGSERNAYATFGRDT
0 2 S135-p PGELSLSsPGPLsPS
2 4 S140-p LSsPGPLsPSGIKSS
0 58 Y150 GIKSSSQYYPSFPSN
0 3 Y151 IKSSSQYYPSFPSNP
0 3 F154 SSQYYPSFPSNPRRR
0 1 N157 YYPSFPSNPRRRAAD
0 2 K173 GLDTQPKKVRKVPPG
1 0 S246 PMLGDGSSPLPLAPG
0 1 S336-p ALGKALAsIysPDHs
0 1 Y338-p GKALAsIysPDHssN
1 4 S339-p KALAsIysPDHssNN
0 2 S343-p sIysPDHssNNFsPs
0 5 S344-p IysPDHssNNFsPsP
0 7 S348-p DHssNNFsPsPstPV
0 8 P349 HssNNFsPsPstPVG
2 4 S350-p ssNNFsPsPstPVGs
0 11 S352-p NNFsPsPstPVGsPQ
3 23 T353-p NFsPsPstPVGsPQG
2 29 S357-p sPstPVGsPQGLPGt
0 5 T364-p sPQGLPGtsQWPrAG
0 2 S365-p PQGLPGtsQWPrAGA
0 1 R369-m1 PGtsQWPrAGAPSAL
1 26 S377-p AGAPSALsPNyDAGL
0 2 N379 APSALsPNyDAGLHG
0 2 Y380-p PSALsPNyDAGLHGL
0 3 T523 KDLKVPRTRTSPDED
0 44 T525 LKVPRTRTSPDEDED
0 33 S526 KVPRTRTSPDEDEDD
1 292 - gap
0 58 - gap
0 2 - gap
0 2 S579 MCQLHLSSEKPQTKL
0 3 K611 RERNLNPKAACLKRR
  E2A iso2  
K35 PLPVANGKSRPASLG
S40 NGKSRPASLGGTQFA
Y109 KGSERNAYATFGRDT
S135 PGELSLSSPGPLSPS
S140 LSSPGPLSPSGIKSS
Y150 GIKSSSQYYPSFPSN
Y151 IKSSSQYYPSFPSNP
F154 SSQYYPSFPSNPRRR
N157 YYPSFPSNPRRRAAD
K172 GGLDTQPKKVRKVPP
S246 PMLGDGSSPLPLAPG
S336 ALGKALASIySPDHs
Y338-p GKALASIySPDHssN
S339 KALASIySPDHssNN
S343-p SIySPDHssNNFSPS
S344-p IySPDHssNNFSPSP
S348 DHssNNFSPSPstPV
P349 HssNNFSPSPstPVG
S350 ssNNFSPSPstPVGs
S352-p NNFSPSPstPVGsPQ
T353-p NFSPSPstPVGsPQG
S357-p SPstPVGsPQGLPGT
T364 sPQGLPGTSQWPRAG
S365 PQGLPGTSQWPRAGA
R369 PGTSQWPRAGAPSAL
S377-p AGAPSALsPNyDAGL
N379 APSALsPNyDAGLHG
Y380-p PSALsPNyDAGLHGL
T523-p KDLKVPRtRtsstDE
T525-p LKVPRtRtsstDEVL
S526-p KVPRtRtsstDEVLs
S527-p VPRtRtsstDEVLsL
T528-p PRtRtsstDEVLsLE
S533-p sstDEVLsLEEKDLR
S576 MCQLHLKSDKAQTKL
K608 RERNLNPKAACLKRR
  human

► Hide Isoforms
 
K34-a PLPVTNGkGRPAsLA
S39-p NGkGRPAsLAGAQFG
Y108-p KSGERGAyASFGRDA
S134-p SGELALNsPGPLsPS
S139-p LNsPGPLsPSGMKGT
Y149-p GMKGTSQyyPSySGs
Y150-p MKGTSQyyPSySGsS
Y153-p TSQyyPSySGsSRRR
S156-p yyPSySGsSRRRAAD
K171-a GSLDTQPkKVRKVPP
S245-p PMLGGGSsPLPLPPG
S338 ALGKALASIYsPDHs
Y340 GKALASIYsPDHssN
S341-p KALASIYsPDHssNN
S345-p SIYsPDHssNNFsss
S346-p IYsPDHssNNFsssP
S350-p DHssNNFsssPstPV
S351-p HssNNFsssPstPVG
S352-p ssNNFsssPstPVGs
S354-p NNFsssPstPVGsPQ
T355-p NFsssPstPVGsPQG
S359-p sPstPVGsPQGLAGt
T366-p sPQGLAGtsQWPRAG
S367-p PQGLAGtsQWPRAGA
R371 AGtsQWPRAGAPGAL
S379-p AGAPGALsPsyDGGL
S381-p APGALsPsyDGGLHG
Y382-p PGALsPsyDGGLHGL
A526 KELKAPRARTsPDED
T528 LKAPRARTsPDEDED
S529-p KAPRARTsPDEDEDD
- gap
- gap
- gap
S582 MCQLHLNSEKPQTKL
K614-u RERNLNPkAACLKRR
  E2A iso2  
K34 PLPVTNGKGRPASLA
S39 NGKGRPASLAGAQFG
Y108 KSGERGAYASFGRDA
S134 SGELALNSPGPLSPS
S139 LNSPGPLSPSGMKGT
Y149-p GMKGTSQyYPSYSGS
Y150 MKGTSQyYPSYSGSS
Y153 TSQyYPSYSGSSRRR
S156 yYPSYSGSSRRRAAD
K171 GSLDTQPKKVRKVPP
S245 PMLGGGSSPLPLPPG
S338 ALGKALASIYSPDHS
Y340 GKALASIYSPDHSSN
S341 KALASIYSPDHSSNN
S345 SIYSPDHSSNNFSSs
S346 IYSPDHSSNNFSSsP
S350 DHSSNNFSSsPStPV
S351 HSSNNFSSsPStPVG
S352-p SSNNFSSsPStPVGs
S354 NNFSSsPStPVGsPQ
T355-p NFSSsPStPVGsPQG
S359-p sPStPVGsPQGLAGT
T366 sPQGLAGTSQWPRAG
S367 PQGLAGTSQWPRAGA
R371 AGTSQWPRAGAPGAL
S379 AGAPGALSPSYDGGL
S381 APGALSPSYDGGLHG
Y382 PGALSPSYDGGLHGL
A526 KELKAPRARtsstDE
T528-p LKAPRARtsstDEVL
S529-p KAPRARtsstDEVLs
S530-p APRARtsstDEVLsL
T531-p PRARtsstDEVLsLE
S536-p sstDEVLsLEEKDLR
S579-p MCQMHLKsDKAQTKL
K611 RERNLNPKAACLKRR
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