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Protein Page:
E2A (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
E2A a transcription factor that plays major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Heterodimers between E2A and tissue-specific basic helix-loop-helix (bHLH) Dimers bind DNA on E-box motifs: 5'- CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Deletions in E2A have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Two alternatively spliced human isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Transcription factor; DNA binding protein
Cellular Component: nucleoplasm; transcription factor complex; protein complex; cytoplasm; nuclear chromatin; nucleus
Molecular Function: protein binding; protein homodimerization activity; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; transcription coactivator activity; bHLH transcription factor binding; chromatin binding; mitogen-activated protein kinase kinase kinase binding; transcription factor binding; transcription factor activity
Biological Process: Peyer's patch development; response to drug; transcription, DNA-dependent; protein stabilization; immunoglobulin V(D)J recombination; B cell lineage commitment; positive regulation of transcription, DNA-dependent; T cell differentiation in the thymus; positive regulation of cell cycle; response to lipopolysaccharide; negative regulation of transcription from RNA polymerase II promoter; muscle cell differentiation; regulation of transcription, DNA-dependent; natural killer cell differentiation; B cell differentiation; positive regulation of B cell proliferation; positive regulation of muscle cell differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; cell development
Reference #:  P15923 (UniProtKB)
Alt. Names/Synonyms: BHLHB21; Class B basic helix-loop-helix protein 21; E2A; E2A immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; ITF1; Kappa-E2-binding factor; MGC129647; MGC129648; TCF-3; TCF3; TFE2; Transcription factor 3; transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47); Transcription factor E2-alpha; Transcription factor ITF-1; VDIR; VDR interacting repressor
Gene Symbols: TCF3
Molecular weight: 67,600 Da
Basal Isoelectric point: Predict pI for various phosphorylation states
CST Pathways:  Adherens Junction Dynamics  |  Wnt/├č-Catenin Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

E2A

Protein Structure Not Found.


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Sites Implicated In
cell growth, altered: S139‑p, S245‑p
transcription, inhibited: S341‑p, S352‑p, T355‑p, S359‑p, S379‑p
activity, inhibited: S341‑p, S352‑p, T355‑p, S359‑p, S379‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
1 0 K34-ac PLPVTNGkGRPAsLA
0 1 S39-p NGkGRPAsLAGAQFG
0 1 A43 RPAsLAGAQFGGSGL
0 6 Y108-p KSGERGAyASFGRDA
0 3 S134-p SGELALNsPGPLsPs
2 6 S139-p LNsPGPLsPsGMKGT
0 1 S141-p sPGPLsPsGMKGTSQ
0 50 Y149-p GMKGTSQyyPSySGs
0 2 Y150-p MKGTSQyyPSySGsS
0 2 Y153-p TSQyyPSySGsSRRR
0 1 S156-p yyPSySGsSRRRAAD
0 2 K171-ac GSLDTQPkKVRKVPP
1 0 S245-p PMLGGGSsPLPLPPG
0 1 S338-p ALGKALAsIysPDHs
0 1 Y340-p GKALAsIysPDHssN
1 4 S341-p KALAsIysPDHssNN
0 2 S345-p sIysPDHssNNFsss
0 6 S346-p IysPDHssNNFsssP
0 6 S350-p DHssNNFsssPstPV
0 8 S351-p HssNNFsssPstPVG
2 4 S352-p ssNNFsssPstPVGs
0 11 S354-p NNFsssPstPVGsPQ
3 24 T355-p NFsssPstPVGsPQG
2 31 S359-p sPstPVGsPQGLAGt
0 5 T366-p sPQGLAGtsQWPRAG
0 2 S367-p PQGLAGtsQWPRAGA
0 1 R371 AGtsQWPRAGAPGAL
0 1 G376 WPRAGAPGALsPsyD
1 28 S379-p AGAPGALsPsyDGGL
0 2 S381-p APGALsPsyDGGLHG
0 2 Y382-p PGALsPsyDGGLHGL
0 1 T411-p LRSHAVGtAGDMHTL
0 3 A526 KELKAPRARtsPDED
0 45 T528-p LKAPRARtsPDEDED
0 34 S529-p KAPRARtsPDEDEDD
1 315 - gap
0 58 - gap
0 2 - gap
0 1 K570 RDINEAFKELGRMCQ
0 1 K570-ub RDINEAFkELGRMCQ
0 2 S582 MCQLHLNSEKPQTKL
0 3 K614-ub RERNLNPkAACLKRR
  E2A iso2  
K34 PLPVTNGKGRPASLA
S39 NGKGRPASLAGAQFG
A43 RPASLAGAQFGGSGL
Y108 KSGERGAYASFGRDA
S134 SGELALNSPGPLSPS
S139 LNSPGPLSPSGMKGT
S141 SPGPLSPSGMKGTSQ
Y149-p GMKGTSQyYPSYSGS
Y150 MKGTSQyYPSYSGSS
Y153 TSQyYPSYSGSSRRR
S156 yYPSYSGSSRRRAAD
K171 GSLDTQPKKVRKVPP
S245 PMLGGGSSPLPLPPG
S338 ALGKALASIYSPDHS
Y340 GKALASIYSPDHSSN
S341 KALASIYSPDHSSNN
S345 SIYSPDHSSNNFSSs
S346 IYSPDHSSNNFSSsP
S350 DHSSNNFSSsPStPV
S351 HSSNNFSSsPStPVG
S352-p SSNNFSSsPStPVGs
S354 NNFSSsPStPVGsPQ
T355-p NFSSsPStPVGsPQG
S359-p sPStPVGsPQGLAGT
T366 sPQGLAGTSQWPRAG
S367 PQGLAGTSQWPRAGA
R371 AGTSQWPRAGAPGAL
G376 WPRAGAPGALSPSYD
S379 AGAPGALSPSYDGGL
S381 APGALSPSYDGGLHG
Y382 PGALSPSYDGGLHGL
T411 LRSHAVGTAGDMHTL
A526 KELKAPRARtsstDE
T528-p LKAPRARtsstDEVL
S529-p KAPRARtsstDEVLs
S530-p APRARtsstDEVLsL
T531-p PRARtsstDEVLsLE
S536-p sstDEVLsLEEKDLR
R567 RDINEAFRELGRMCQ
R567 RDINEAFRELGRMCQ
S579-p MCQMHLKsDKAQTKL
K611 RERNLNPKAACLKRR
  mouse

► Hide Isoforms
 
K35 PLPVANGKSRPASLG
S40 NGKSRPASLGGtQFA
T44-p RPASLGGtQFAGSGL
Y109 KGSERNAYATFGRDT
S135-p PGELSLSsPGPLsPS
S140-p LSsPGPLsPSGIKSS
S142 sPGPLsPSGIKSSSQ
Y150 GIKSSSQYYPSFPSN
Y151 IKSSSQYYPSFPSNP
F154 SSQYYPSFPSNPRRR
N157 YYPSFPSNPRRRAAD
K173 GLDTQPKKVRKVPPG
S246 PMLGDGSSPLPLAPG
S336-p ALGKALAsIysPDHs
Y338-p GKALAsIysPDHssN
S339-p KALAsIysPDHssNN
S343-p sIysPDHssNNFsPs
S344-p IysPDHssNNFsPsP
S348-p DHssNNFsPsPstPV
P349 HssNNFsPsPstPVG
S350-p ssNNFsPsPstPVGs
S352-p NNFsPsPstPVGsPQ
T353-p NFsPsPstPVGsPQG
S357-p sPstPVGsPQGLPGt
T364-p sPQGLPGtsQWPrAG
S365-p PQGLPGtsQWPrAGA
R369-m1 PGtsQWPrAGAPsAL
S374-p WPrAGAPsALsPNyD
S377-p AGAPsALsPNyDAGL
N379 APsALsPNyDAGLHG
Y380-p PsALsPNyDAGLHGL
T408 LRSHAVGTASDLHGL
T523 KDLKVPRTRTSPDED
T525 LKVPRTRTSPDEDED
S526 KVPRTRTSPDEDEDD
- gap
- gap
- gap
K567-ac RDINEAFkELGRMCQ
K567 RDINEAFKELGRMCQ
S579 MCQLHLSSEKPQTKL
K611 RERNLNPKAACLKRR
  E2A iso2  
K35 PLPVANGKSRPASLG
S40 NGKSRPASLGGTQFA
T44 RPASLGGTQFAGSGL
Y109 KGSERNAYATFGRDT
S135 PGELSLSSPGPLSPS
S140 LSSPGPLSPSGIKSS
S142 SPGPLSPSGIKSSSQ
Y150 GIKSSSQYYPSFPSN
Y151 IKSSSQYYPSFPSNP
F154 SSQYYPSFPSNPRRR
N157 YYPSFPSNPRRRAAD
K172 GGLDTQPKKVRKVPP
S246 PMLGDGSSPLPLAPG
S336 ALGKALASIySPDHs
Y338-p GKALASIySPDHssN
S339 KALASIySPDHssNN
S343-p SIySPDHssNNFSPS
S344-p IySPDHssNNFSPSP
S348 DHssNNFSPSPstPV
P349 HssNNFSPSPstPVG
S350 ssNNFSPSPstPVGs
S352-p NNFSPSPstPVGsPQ
T353-p NFSPSPstPVGsPQG
S357-p SPstPVGsPQGLPGT
T364 sPQGLPGTSQWPRAG
S365 PQGLPGTSQWPRAGA
R369 PGTSQWPRAGAPSAL
S374 WPRAGAPSALsPNyD
S377-p AGAPSALsPNyDAGL
N379 APSALsPNyDAGLHG
Y380-p PSALsPNyDAGLHGL
T408 LRSHAVGTASDLHGL
T523-p KDLKVPRtRtsstDE
T525-p LKVPRtRtsstDEVL
S526-p KVPRtRtsstDEVLs
S527-p VPRtRtsstDEVLsL
T528-p PRtRtsstDEVLsLE
S533-p sstDEVLsLEEKDLR
R564 RDINEAFRELGRMCQ
R564 RDINEAFRELGRMCQ
S576 MCQLHLKSDKAQTKL
K608 RERNLNPKAACLKRR
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