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Protein Page:
Cot (human)

Overview
Cot an oncogenic Ser/Thr kinase of the STE group. Activates IkappaB kinases, thus inducing the nuclear translocation of NF-kappaB. Promotes the production of TNF-alpha and IL-2 during T lymphocyte activation. Interacts with NFkB-p105. Overexpressed and amplified in breast tumors. Viral insertions induce rat lymphomas and mouse mammary carcinomas. Isolated as a transforming factor in two cell lines. Mediates LPS activation of macrophages. 2 isoforms of the human protein are produced by alternative initiation. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.25; STE group; STE-Unique family
Cellular Component: cytosol
Molecular Function: protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; magnesium ion binding; ATP binding
Biological Process: activation of MAPKK activity; T cell costimulation; MAPKKK cascade; cell cycle; protein amino acid phosphorylation
Reference #:  P41279 (UniProtKB)
Alt. Names/Synonyms: c-COT; Cancer Osaka thyroid oncogene; COT; cot (cancer Osaka thyroid) oncogene; EST; ESTF; Ewing sarcoma transformant; FLJ10486; M3K8; MAP3K8; MEKK8; Mitogen-activated protein kinase kinase kinase 8; Proto-oncogene c-Cot; proto-oncogene serine/threoine protein kinase; Serine/threonine-protein kinase cot; TPL-2; TPL2; Tumor progression locus 2; tumor progression locus-2
Gene Symbols: MAP3K8
Molecular weight: 52,925 Da
Basal Isoelectric point: 5.54  Predict pI for various phosphorylation states
CST Pathways:  Actin Dynamics  |  B Cell Receptor Signaling  |  Growth And Differentiation Control by MAPKs  |  NF-kB Signaling  |  PI3K/Akt Signaling  |  SAPK/JNK Signaling Cascades
Select Structure to View Below

Cot

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
transcription, altered: S400‑p
enzymatic activity, induced: S62‑p, T290‑p
molecular association, regulation: T290‑p
phosphorylation: T290‑p
protein degradation: T290‑p

Modification Sites and Domains  

Modification Sites in Parent Protein, Orthologs, and Isoforms  
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
1 1 S62-p QNDERSKsLLLSGQE
0 1 T80 LSSVRYGTVEDLLAF
0 1 S125-p NGRYQIDsDVLLIPW
0 15 S141-p LTYRNIGsDFIPRGA
5 0 T290-p FPKDLRGtEIYMSPE
0 1 S334-p WVKRYPRsAYPSYLY
2 1 S400-p EDQPRCQsLDSALLE
1 0 S413 LERKRLLSRKELELP
0 1 S443 EMLKRQRSLYIDLGA
4491 : Phospho-Tpl2 (Ser400) Antibody
  mouse

 
S62 QNEERSESLLRSGQE
T80 LSSVRYGTVEDLLAF
S125 NGRYQIDSDVLLVPW
S141-p LTYRNIGsGFVPRGA
T290-p LPKDLRGtEIYMSPE
S334 WVKRYPRSAYPSYLY
S400-p EDQPRCQsLDSALFE
S413-p FERKRLLsRKELQLP
S443 EVLRRQRSLYIDLGA
4491 : Phospho-Tpl2 (Ser400) Antibody
  rat

 
S62 QNKEHSESLLRSGQE
T80-p LSSVRYGtVEDLLAF
S125 NGRYQIDSDVLLVPW
S141 LTYRSIGSGFVPRGA
T290 LPKDLRGTEIYMSPE
S334 WVKRYPRSAYPSYLY
S400-p EDQPRCQsLDSALFD
S413 FDRKRLLSRKELELP
S443-p EVLRRQRsLYIDLGA
4491 : Phospho-Tpl2 (Ser400) Antibody
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