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Protein Page:
PSD-95 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PSD-95 a membrane associated guanylate kinase (MAGUK) scaffolding protein located in neural postsynaptic densities. Associates with NMDA receptor NR2 subunits via glutamate serine (aspartate/glutamate) valine motifs in the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Its overexpression or depletion changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells. May reduce the amplitude of ACCN3 acid-evoked currents by retaining the channel intracellularly. Binds tissue-type plasminogen activator (tPA), mediating neural NMDA gating via a complex of LRP1, PSD-95 and NMDAR. Interacts through its first two PDZ domains with NMDAR2A, NMDAR2B, NMDAR2C, NMDAR2D, ACCN3, certain splice forms of NMDAR1, KV4.2, CXADR and synGAP. Interacts through its first two PDZ domains with Kv1.1, Kv1.2, Kv1.3, Kv1.4 and HER4. Interacts through its first PDZ domain with GluR6 and Kv1.4. Interacts through its second PDZ domain with the PDZ domain of nNOS or the C-terminus of NOS1AP. May interact with 5-HT(2A). Interacts through its third PDZ domain with NLGN1, and probably with NLGN2 and NLGN3. Interacts through its guanylate kinase-like domain with SAPAP1, SAPAP2, SAPAP3, SAPAP4, MAP1A, and BEGAIN. Interacts through its guanylate kinase-like domain with KIF13B. Two alternatively spliced human isoforms have been reported. Palmitoylation of isoform 1 is required for targeting to postsynaptic density. Note: This description may include information from UniProtKB.
Protein type: Adaptor/scaffold
Cellular Component: cortical cytoskeleton; dendrite cytoplasm; endoplasmic reticulum; postsynaptic density; excitatory synapse; postsynaptic membrane; synaptic vesicle; extrinsic to internal side of plasma membrane; cytoplasm; plasma membrane; ionotropic glutamate receptor complex; synapse; cell junction
Molecular Function: P2Y1 nucleotide receptor binding; protein C-terminus binding; ionotropic glutamate receptor binding; protein binding; beta-1 adrenergic receptor binding; protein complex binding; acetylcholine receptor binding; D1 dopamine receptor binding; protein phosphatase binding; PDZ domain binding
Biological Process: regulation of long-term neuronal synaptic plasticity; axon guidance; nervous system development; synaptic vesicle maturation; social behavior; learning; signal transduction; positive regulation of synaptic transmission; synaptic transmission; elevation of cytosolic calcium ion concentration; establishment of protein localization; protein complex assembly; neuromuscular process controlling balance; negative regulation of receptor internalization
Reference #:  P78352 (UniProtKB)
Alt. Names/Synonyms: discs large homolog 4; discs, large homolog 4 (Drosophila); Disks large homolog 4; DLG4; FLJ97752; FLJ98574; post-synaptic density protein 95; Postsynaptic density protein 95; PSD-95; PSD95; SAP-90; SAP90; Synapse-associated protein 90; Tax interaction protein 15
Gene Symbols: DLG4
Molecular weight: 80,495 Da
Basal Isoelectric point: 5.58  Predict pI for various phosphorylation states
CST Pathways:  ErbB/HER Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PSD-95

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 2 T8-p MDCLCIVtTKKYRYQ
3 0 T19 YRYQDEDTPPLEHSP
2 0 S25 DTPPLEHSPAHLPNQ
0 1 T67 EMEYEEITLERGNSG
2 3 S73 ITLERGNSGLGFSIA
0 16 K138 SAAVEALKEAGsIVR
0 9 S142-p EALKEAGsIVRLyVM
0 1 Y147-p AGsIVRLyVMRRKPP
0 1 K157 RRKPPAEKVMEIKLI
0 7 K162 AEKVMEIKLIKGPKG
0 4 K168 IKLIKGPKGLGFSIA
0 23 Y190 IPGDNSIYVtKIIEG
0 2 T192-p GDNSIYVtKIIEGGA
0 20 K202 IEGGAAHKDGRLQIG
1 19 Y236 VAALKNTYDVVYLKV
1 118 Y240 KNTYDVVYLKVAKPS
0 1 K242 TYDVVYLKVAKPSNA
0 2 Y255 NAYLSDSYAPPDITT
0 1 Y264 PPDITTSYSQHLDNE
0 1 S276 DNEISHSSYLGTDYP
0 1 Y282 SSYLGTDYPTAMTPT
0 1 T284 YLGTDYPTAMTPTSP
1 2 T287 TDYPTAMTPTSPRRY
1 4 S290 PTAMTPTSPRRYsPV
3 4 S295-p PTSPRRYsPVAKDLL
0 1 K299 RRYsPVAKDLLGEED
0 1 K355 DLSGELRKGDQILSV
2 8 Y397-p AQYKPEEySRFEAKI
0 2 S398 QYKPEEySRFEAKIH
0 3 K403 EySRFEAKIHDLREQ
0 2 S414 LREQLMNSSLGSGTA
1 9 S415 REQLMNSSLGSGTAS
1 10 S418 LMNSSLGSGTASLRS
0 3 T420 NSSLGSGTASLRSNP
0 6 S422 SLGSGTASLRSNPKR
0 3 S425 SGTASLRSNPKRGFY
0 3 Y432 SNPKRGFYIRALFDY
0 34 Y439 YIRALFDYDKTKDCG
0 2 T442 ALFDYDKTKDCGFLS
0 1 K443 LFDYDKTKDCGFLSQ
0 2 S449 TKDCGFLSQALSFRF
0 1 S478 WQARRVHSDSETDDI
0 2 S480 ARRVHSDSETDDIGF
0 3 K505 EWSRLKAKDWGSSSG
0 1 S509 LKAKDWGSSSGSQGR
0 2 S513 DWGSSSGSQGREDSV
3 0 Y523-p REDSVLSyETVTQME
1 2 Y533 VTQMEVHYARPIIIL
0 1 K544 IIILGPTKDRANDDL
0 14 K558 LLSEFPDKFGsCVPH
1 1 S561-p EFPDKFGsCVPHTTR
0 9 Y573-p TTRPKREyEIDGRDY
0 29 Y580 yEIDGRDYHFVSSRE
0 1 S584 GRDYHFVSSREKMEK
0 3 S585 RDYHFVSSREKMEKD
0 1 K588 HFVSSREKMEKDIQA
0 14 K591 SSREKMEKDIQAHKF
0 2 K597 EKDIQAHKFIEAGQY
0 82 Y604 KFIEAGQYNSHLYGT
0 7 S606 IEAGQYNSHLYGTSV
0 41 Y609 GQYNSHLYGTSVQSV
0 2 S654 AIFIRPRSLENVLEI
0 8 K663 ENVLEINKRITEEQA
0 1 K672 ITEEQARKAFDRATK
0 1 Y701-p GDSFEEIyHKVKRVI
0 24 Y715 IEDLSGPYIWVPARE
3919 : Phospho-PSD95 (Tyr236/Tyr240) Antibody
3919 : Phospho-PSD95 (Tyr236/Tyr240) Antibody
  mouse

 
T8 MDCLCIVTTKKYRYQ
T19-p YRYQDEDtPPLEHsP
S25-p DtPPLEHsPAHLPNQ
T67-p EMEYEEItLERGNsG
S73-p ItLERGNsGLGFSIA
K138-ub SAAVEALkEAGsIVR
S142-p EALkEAGsIVRLYVM
Y147 AGsIVRLYVMRRKPP
K157 RRKPPAEKIIEIkLI
K162-ub AEKIIEIkLIKGPkG
K168-ub IkLIKGPkGLGFSIA
Y190 IPGDNSIYVTKIIEG
T192 GDNSIYVTKIIEGGA
K202-ub IEGGAAHkDGRLQIG
Y236-p VAALKNTyDVVyLkV
Y240-p KNTyDVVyLkVAKPS
K242-ub TyDVVyLkVAKPSNA
Y255 NAYLSDSYAPPDITT
Y264 PPDITTSYSQHLDNE
S276 DNEISHSSYLGTDYP
Y282 SSYLGTDYPTAMtPT
T284 YLGTDYPTAMtPTsP
T287-p TDYPTAMtPTsPRRY
S290-p PTAMtPTsPRRYsPV
S295-p PTsPRRYsPVAkDLL
K299-ub RRYsPVAkDLLGEED
K355-ub DLSGELRkGDQILSV
Y397-p AQYKPEEysRFEAkI
S398-p QYKPEEysRFEAkIH
K403-ub EysRFEAkIHDLREQ
S414-p LREQLMNssLGsGtA
S415-p REQLMNssLGsGtAs
S418-p LMNssLGsGtAsLRs
T420-p NssLGsGtAsLRsNP
S422-p sLGsGtAsLRsNPKR
S425-p sGtAsLRsNPKRGFy
Y432-p sNPKRGFyIRALFDY
Y439 yIRALFDYDKtKDCG
T442-p ALFDYDKtKDCGFLS
K443 LFDYDKtKDCGFLSQ
S449 tKDCGFLSQALSFHF
S478-p WQARRVHsDsETDDI
S480-p ARRVHsDsETDDIGF
K505-ub EWSRLKAkDWGsSSG
S509-p LKAkDWGsSSGsQGR
S513-p DWGsSSGsQGREDSV
Y523 REDSVLSYETVTQME
Y533 VTQMEVHYARPIIIL
K544 IIILGPTKDRANDDL
K558-ub LLSEFPDkFGsCVPH
S561-p EFPDkFGsCVPHTTR
Y573 TTRPKREYEIDGRDy
Y580-p YEIDGRDyHFVSSRE
S584 GRDyHFVSSREkMEk
S585 RDyHFVSSREkMEkD
K588-ub HFVSSREkMEkDIQA
K591-ub SSREkMEkDIQAHKF
K597 EkDIQAHKFIEAGQy
Y604-p KFIEAGQyNsHLyGT
S606-p IEAGQyNsHLyGTSV
Y609-p GQyNsHLyGTSVQSV
S654-p AIFIRPRsLENVLEI
K663-ub ENVLEINkRITEEQA
K672-ac ITEEQARkAFDRATK
Y701 GDSFEEIYHKVKRVI
Y715-p IEDLSGPyIWVPARE
3919 : Phospho-PSD95 (Tyr236/Tyr240) Antibody
3919 : Phospho-PSD95 (Tyr236/Tyr240) Antibody
  rat

 
T8 MDCLCIVTTKKYRYQ
T19-p YRYQDEDtPPLEHSP
S25 DtPPLEHSPAHLPNQ
T67 EMEYEEITLERGNsG
S73-p ITLERGNsGLGFSIA
K138-ub SAAVEALkEAGSIVR
S142 EALkEAGSIVRLYVM
Y147 AGSIVRLYVMRRKPP
K157-ub RRKPPAEkVMEIKLI
K162 AEkVMEIKLIKGPKG
K168 IKLIKGPKGLGFSIA
Y190-p IPGDNSIyVtKIIEG
T192-p GDNSIyVtKIIEGGA
K202-ub IEGGAAHkDGRLQIG
Y236-p VAALKNTyDVVyLKV
Y240-p KNTyDVVyLKVAKPS
K242 TyDVVyLKVAKPSNA
Y255-p NAYLSDSyAPPDITT
Y264-p PPDITTSySQHLDNE
S276-p DNEISHSsYLGTDyP
Y282-p SsYLGTDyPtAMtPT
T284-p YLGTDyPtAMtPTsP
T287-p TDyPtAMtPTsPRRY
S290-p PtAMtPTsPRRYsPV
S295-p PTsPRRYsPVAKDLL
K299 RRYsPVAKDLLGEED
K355 DLSGELRKGDQILSV
Y397-p AQYKPEEysRFEAKI
S398-p QYKPEEysRFEAKIH
K403 EysRFEAKIHDLREQ
S414 LREQLMNSsLGsGTA
S415-p REQLMNSsLGsGTAS
S418-p LMNSsLGsGTASLRS
T420 NSsLGsGTASLRSNP
S422 sLGsGTASLRSNPKR
S425 sGTASLRSNPKRGFy
Y432-p SNPKRGFyIRALFDy
Y439-p yIRALFDyDKtkDCG
T442-p ALFDyDKtkDCGFLs
K443-ub LFDyDKtkDCGFLsQ
S449-p tkDCGFLsQALSFRF
S478 WQARRVHSDSETDDI
S480 ARRVHSDSETDDIGF
K505 EWSRLKAKDWGSSSG
S509 LKAKDWGSSSGSQGR
S513 DWGSSSGSQGREDSV
Y523-p REDSVLSyETVTQME
Y533-p VTQMEVHyARPIIIL
K544-ac IIILGPTkDRANDDL
K558-ub LLSEFPDkFGSCVPH
S561 EFPDkFGSCVPHTTR
Y573-p TTRPKREyEIDGRDy
Y580-p yEIDGRDyHFVssRE
S584-p GRDyHFVssREKMEk
S585-p RDyHFVssREKMEkD
K588 HFVssREKMEkDIQA
K591-ub ssREKMEkDIQAHkF
K597-ub EkDIQAHkFIEAGQy
Y604-p kFIEAGQyNsHLyGT
S606-p IEAGQyNsHLyGTSV
Y609-p GQyNsHLyGTSVQSV
S654 AIFIRPRSLENVLEI
K663-ub ENVLEINkRITEEQA
K672 ITEEQARKAFDRATK
Y701 GDSFEEIYHKVKRVI
Y715-p IEDLSGPyIWVPARE
3919 : Phospho-PSD95 (Tyr236/Tyr240) Antibody
3919 : Phospho-PSD95 (Tyr236/Tyr240) Antibody
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