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Protein Page:
PSD-95 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
PSD-95 a membrane associated guanylate kinase (MAGUK) scaffolding protein located in neural postsynaptic densities. Associates with NMDA receptor NR2 subunits via glutamate serine (aspartate/glutamate) valine motifs in the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Its overexpression or depletion changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells. May reduce the amplitude of ACCN3 acid-evoked currents by retaining the channel intracellularly. Binds tissue-type plasminogen activator (tPA), mediating neural NMDA gating via a complex of LRP1, PSD-95 and NMDAR. Interacts through its first two PDZ domains with NMDAR2A, NMDAR2B, NMDAR2C, NMDAR2D, ACCN3, certain splice forms of NMDAR1, KV4.2, CXADR and synGAP. Interacts through its first two PDZ domains with Kv1.1, Kv1.2, Kv1.3, Kv1.4 and HER4. Interacts through its first PDZ domain with GluR6 and Kv1.4. Interacts through its second PDZ domain with the PDZ domain of nNOS or the C-terminus of NOS1AP. May interact with 5-HT(2A). Interacts through its third PDZ domain with NLGN1, and probably with NLGN2 and NLGN3. Interacts through its guanylate kinase-like domain with SAPAP1, SAPAP2, SAPAP3, SAPAP4, MAP1A, and BEGAIN. Interacts through its guanylate kinase-like domain with KIF13B. Two alternatively spliced human isoforms have been reported. Palmitoylation of isoform 1 is required for targeting to postsynaptic density. Note: This description may include information from UniProtKB.
Protein type: Adaptor/scaffold
Cellular Component: dendrite cytoplasm; cortical cytoskeleton; postsynaptic membrane; synaptic vesicle; extrinsic to internal side of plasma membrane; endoplasmic reticulum; postsynaptic density; cytoplasm; plasma membrane; ionotropic glutamate receptor complex; excitatory synapse; synapse; cell junction
Molecular Function: protein C-terminus binding; P2Y1 nucleotide receptor binding; ionotropic glutamate receptor binding; protein binding; beta-1 adrenergic receptor binding; acetylcholine receptor binding; protein complex binding; protein phosphatase binding; D1 dopamine receptor binding; PDZ domain binding
Biological Process: regulation of long-term neuronal synaptic plasticity; axon guidance; nervous system development; synaptic vesicle maturation; social behavior; learning; signal transduction; positive regulation of synaptic transmission; synaptic transmission; establishment of protein localization; elevation of cytosolic calcium ion concentration; protein complex assembly; neuromuscular process controlling balance; negative regulation of receptor internalization
Reference #:  P78352 (UniProtKB)
Alt. Names/Synonyms: discs large homolog 4; discs, large homolog 4 (Drosophila); Disks large homolog 4; DLG4; FLJ97752; FLJ98574; post-synaptic density protein 95; Postsynaptic density protein 95; PSD-95; PSD95; SAP-90; SAP90; Synapse-associated protein 90; Tax interaction protein 15
Gene Symbols: DLG4
Molecular weight: 80,495 Da
Basal Isoelectric point: 5.58  Predict pI for various phosphorylation states
CST Pathways:  ErbB/HER Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PSD-95

Protein Structure Not Found.


STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB  |  Phospho3D  |  Source  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene


Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 2 T8-p MDCLCIVtTKKYRYQ
3 0 T19 YRYQDEDTPPLEHSP
2 0 S25 DTPPLEHSPAHLPNQ
0 1 T67 EMEYEEITLERGNSG
2 3 S73 ITLERGNSGLGFSIA
0 16 K138 SAAVEALKEAGsIVR
0 9 S142-p EALKEAGsIVRLYVM
0 7 K162 AEKVMEIKLIKGPKG
0 4 K168 IKLIKGPKGLGFSIA
0 25 Y190 IPGDNSIYVTKIIEG
0 20 K202 IEGGAAHKDGRLQIG
0 20 Y236-p VAALKNTyDVVyLKV
0 119 Y240-p KNTyDVVyLKVAKPS
0 1 K242 TyDVVyLKVAKPSNA
1 0 T287 TDYPTAMTPTSPRRY
1 1 S290 PTAMTPTSPRRYsPV
3 4 S295-p PTSPRRYsPVAKDLL
0 1 K299 RRYsPVAKDLLGEED
0 1 K355 DLSGELRKGDQILSV
2 23 Y397-p AQYKPEEySRFEAKI
0 3 K403 EySRFEAKIHDLREQ
0 2 S414 LREQLMNSSLGSGTA
1 9 S415 REQLMNSSLGSGTAS
1 10 S418 LMNSSLGSGTASLRS
0 3 T420 NSSLGSGTASLRSNP
0 6 S422 SLGSGTASLRSNPKR
0 3 S425 SGTASLRSNPKRGFY
0 2 Y432 SNPKRGFYIRALFDY
0 35 Y439 YIRALFDYDKTKDCG
0 3 T442 ALFDYDKTKDCGFLS
0 2 S449 TKDCGFLSQALSFRF
0 2 S453 GFLSQALSFRFGDVL
0 1 S478 WQARRVHSDSETDDI
0 2 S480 ARRVHSDSETDDIGF
0 3 K505 EWSRLKAKDWGSSSG
0 1 S509 LKAKDWGSSSGSQGR
0 2 S513 DWGSSSGSQGREDSV
2 0 Y523-p REDSVLSyETVTQME
1 2 Y533 VTQMEVHYARPIIIL
0 14 K558 LLSEFPDKFGsCVPH
1 1 S561-p EFPDKFGsCVPHTTR
0 8 Y573-p TTRPKREyEIDGRDY
0 29 Y580 yEIDGRDYHFVSSRE
0 14 K591 SSREKMEKDIQAHKF
0 2 K597 EKDIQAHKFIEAGQY
0 84 Y604 KFIEAGQYNSHLYGT
0 2 S606 IEAGQYNSHLYGTSV
0 47 Y609 GQYNSHLYGTSVQSV
0 2 S654 AIFIRPRSLENVLEI
0 8 K663 ENVLEINKRITEEQA
0 1 Y701-p GDSFEEIyHKVKRVI
0 24 Y715 IEDLSGPYIWVPARE
3919 : Phospho-PSD95 (Tyr236/Tyr240) Antibody
3919 : Phospho-PSD95 (Tyr236/Tyr240) Antibody
  mouse

 
T8 MDCLCIVTTKKYRYQ
T19-p YRYQDEDtPPLEHsP
S25-p DtPPLEHsPAHLPNQ
T67-p EMEYEEItLERGNsG
S73-p ItLERGNsGLGFSIA
K138-u SAAVEALkEAGsIVR
S142-p EALkEAGsIVRLYVM
K162-u AEKIIEIkLIKGPkG
K168-u IkLIKGPkGLGFSIA
Y190 IPGDNSIYVTKIIEG
K202-u IEGGAAHkDGRLQIG
Y236-p VAALKNTyDVVyLkV
Y240-p KNTyDVVyLkVAKPS
K242-u TyDVVyLkVAKPSNA
T287-p TDYPTAMtPTsPRRY
S290-p PTAMtPTsPRRYsPV
S295-p PTsPRRYsPVAkDLL
K299-u RRYsPVAkDLLGEED
K355-u DLSGELRkGDQILSV
Y397-p AQYKPEEySRFEAkI
K403-u EySRFEAkIHDLREQ
S414-p LREQLMNssLGsGtA
S415-p REQLMNssLGsGtAs
S418-p LMNssLGsGtAsLRs
T420-p NssLGsGtAsLRsNP
S422-p sLGsGtAsLRsNPKR
S425-p sGtAsLRsNPKRGFy
Y432-p sNPKRGFyIRALFDY
Y439 yIRALFDYDKtKDCG
T442-p ALFDYDKtKDCGFLS
S449 tKDCGFLSQALSFHF
S453 GFLSQALSFHFGDVL
S478-p WQARRVHsDsETDDI
S480-p ARRVHsDsETDDIGF
K505-u EWSRLKAkDWGsSSG
S509-p LKAkDWGsSSGsQGR
S513-p DWGsSSGsQGREDSV
Y523 REDSVLSYETVTQME
Y533 VTQMEVHYARPIIIL
K558-u LLSEFPDkFGsCVPH
S561-p EFPDkFGsCVPHTTR
Y573 TTRPKREYEIDGRDy
Y580-p YEIDGRDyHFVSSRE
K591-u SSREKMEkDIQAHKF
K597 EkDIQAHKFIEAGQy
Y604-p KFIEAGQyNsHLyGT
S606-p IEAGQyNsHLyGTSV
Y609-p GQyNsHLyGTSVQSV
S654-p AIFIRPRsLENVLEI
K663-u ENVLEINkRITEEQA
Y701 GDSFEEIYHKVKRVI
Y715-p IEDLSGPyIWVPARE
3919 : Phospho-PSD95 (Tyr236/Tyr240) Antibody
3919 : Phospho-PSD95 (Tyr236/Tyr240) Antibody
  rat

 
T8 MDCLCIVTTKKYRYQ
T19-p YRYQDEDtPPLEHSP
S25 DtPPLEHSPAHLPNQ
T67 EMEYEEITLERGNsG
S73-p ITLERGNsGLGFSIA
K138-u SAAVEALkEAGSIVR
S142 EALkEAGSIVRLYVM
K162 AEKVMEIKLIKGPKG
K168 IKLIKGPKGLGFSIA
Y190-p IPGDNSIyVTKIIEG
K202-u IEGGAAHkDGRLQIG
Y236-p VAALKNTyDVVyLKV
Y240-p KNTyDVVyLKVAKPS
K242 TyDVVyLKVAKPSNA
T287-p TDYPTAMtPTsPRRY
S290-p PTAMtPTsPRRYsPV
S295-p PTsPRRYsPVAKDLL
K299 RRYsPVAKDLLGEED
K355 DLSGELRKGDQILSV
Y397-p AQYKPEEySRFEAKI
K403 EySRFEAKIHDLREQ
S414 LREQLMNSsLGsGTA
S415-p REQLMNSsLGsGTAS
S418-p LMNSsLGsGTASLRS
T420 NSsLGsGTASLRSNP
S422 sLGsGTASLRSNPKR
S425 sGTASLRSNPKRGFy
Y432-p SNPKRGFyIRALFDy
Y439-p yIRALFDyDKtKDCG
T442-p ALFDyDKtKDCGFLs
S449-p tKDCGFLsQALsFRF
S453-p GFLsQALsFRFGDVL
S478 WQARRVHSDSETDDI
S480 ARRVHSDSETDDIGF
K505 EWSRLKAKDWGSSSG
S509 LKAKDWGSSSGSQGR
S513 DWGSSSGSQGREDSV
Y523-p REDSVLSyETVTQME
Y533-p VTQMEVHyARPIIIL
K558-u LLSEFPDkFGSCVPH
S561 EFPDkFGSCVPHTTR
Y573-p TTRPKREyEIDGRDy
Y580-p yEIDGRDyHFVSSRE
K591-u SSREKMEkDIQAHkF
K597-u EkDIQAHkFIEAGQy
Y604-p kFIEAGQyNSHLyGT
S606 IEAGQyNSHLyGTSV
Y609-p GQyNSHLyGTSVQSV
S654 AIFIRPRSLENVLEI
K663-u ENVLEINkRITEEQA
Y701 GDSFEEIYHKVKRVI
Y715-p IEDLSGPyIWVPARE
3919 : Phospho-PSD95 (Tyr236/Tyr240) Antibody
3919 : Phospho-PSD95 (Tyr236/Tyr240) Antibody
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