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Protein Page:
DRP1 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
DRP1 Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into ring-like structures which wrap around the scission site to constict and sever the mitochondrial membrane through a GTP hydrolysis- dependent mechanism. Required for normal brain development. Facilitates developmentally-regulated apoptosis during neural tube development. Required for a normal rate of cytochrome c release and caspase activation during apoptosis. Also required for mitochondrial fission during mitosis. May be involved in vesicle transport. Homotetramer; dimerizes through the N-terminal GTP-middle region of one molecule binding to the GED domain of another DNM1L molecule. Can self-assemble in multimeric ring-like structures. Interacts with BCL2L1; the interaction stimulates the GTPase activity of DMN1L in synapses and increases the number of axonal mitochondria and the size and number of synaptic vesicle clusters. Interacts with FIS1. Interacts with GSK3B and MARCH5. Interacts (via the GTPase and B domains) with UBE2I; the interaction promotes sumoylation of DNM1L, mainly in ite B domain. Interacts with PPP3CA; the interaction dephosphorylates DNM1L and regulates its transition to mitochondria. Interacts witn MID49 and MID51. Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons. Belongs to the dynamin family. 6 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Endoplasmic reticulum; Hydrolase; Mitochondrial; Apoptosis; EC 3.6.5.5; Microtubule binding protein; Motor protein
Cellular Component: Golgi apparatus; microtubule; protein complex; mitochondrion; intracellular membrane-bound organelle; synaptic vesicle membrane; coated pit; cytosol; mitochondrial outer membrane; perinuclear region of cytoplasm; cytoplasm; peroxisome; cell junction
Molecular Function: GTPase activity; identical protein binding; protein binding; protein homodimerization activity; GTP binding; ubiquitin protein ligase binding
Biological Process: peroxisome fission; mitochondrial fission; release of cytochrome c from mitochondria; positive regulation of protein secretion; GTP catabolic process; positive regulation of apoptosis; apoptosis; regulation of protein oligomerization; endocytosis; mitochondrial fragmentation during apoptosis; protein homotetramerization; cell structure disassembly during apoptosis
Reference #:  O00429 (UniProtKB)
Alt. Names/Synonyms: DLP1; DNM1L; Dnm1p/Vps1p-like protein; DRP1; DVLP; Dymple; dynamin 1-like; Dynamin family member proline-rich carboxyl-terminal domain less; Dynamin-1-like protein; Dynamin-like protein; Dynamin-like protein 4; Dynamin-like protein IV; Dynamin-related protein 1; DYNIV-11; FLJ41912; HdynIV; VPS1
Gene Symbols: DNM1L
Molecular weight: 81,877 Da
Basal Isoelectric point: 6.37  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

DRP1

Protein Structure Not Found.


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Sites Implicated In
apoptosis, induced: S616‑p
enzymatic activity, inhibited: S637‑p
intracellular localization: S616‑p, S637‑p
molecular association, regulation: S637‑p
protein conformation: S637‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 S71 ILQLVHVSQEDKRKt
0 2 T78-p SQEDKRKttGEENGV
0 1 T79-p QEDKRKttGEENGVE
0 1 - gap
0 1 K160 VPVGDQPKDIELQIR
0 2 K238-u MGRVIPVkLGIIGVV
0 1 K283-a LANRNGTkYLARTLN
0 1 K305 RDCLPELKTRINVLA
0 2 Y368 CGGARICYIFHETFG
0 1 T394-p LNTIDILtAIRNATG
0 1 D503-ca TKHPDFAdACGLMNN
0 3 S529-p RELPSAVsRDkSSkV
1 0 K532-s PSAVsRDkSSkVPSA
1 0 K535-s VsRDkSSkVPSALAP
0 1 S544-p PSALAPAsQEPsPAA
0 1 S548-p APAsQEPsPAASAEA
1 0 K558-s ASAEADGkLIQDSRR
1 0 K568-s QDSRRETkNVASGGG
0 4 T586-p DGVQEPTtGNWRGML
1 0 K594-s GNWRGMLktSkAEEL
1 0 T595-p NWRGMLktSkAEELL
1 0 K597-s RGMLktSkAEELLAE
1 0 K606-s EELLAEEkskPIPIM
0 7 S607-p ELLAEEkskPIPIMP
1 0 K608-s LLAEEkskPIPIMPA
8 143 S616-p PIPIMPAsPQKGHAV
7 0 S637-p VPVARKLsAREQRDC
1 0 S693-p LVGQLYKsSLLDDLL
3455 : Phospho-DRP1 (Ser616) Antibody
4494 : Phospho-DRP1 (Ser616) (D9A1) Rabbit mAb
4867 : Phospho-DRP1 (Ser637) Antibody
6319 : Phospho-DRP1 (Ser637) (D3A4) Rabbit mAb
  DRP1 iso3  
S71 ILQLVHVSQEDKRKT
T78 SQEDKRKTTGEENGV
T79 QEDKRKTTGEENGVE
- gap
K160 VPVGDQPKDIELQIR
K238 MGRVIPVKLGIIGVV
K283 LANRNGTKYLARTLN
K305 RDCLPELKTRINVLA
Y368 CGGARICYIFHETFG
T394 LNTIDILTAIRNATG
D503 TKHPDFADACGLMNN
S529 RELPSAVSRDKVASG
K532 PSAVSRDKVASGGGG
- gap
- gap
- gap
- gap
- gap
T549-p DGVQEPTtGNWRGML
K557 GNWRGMLKTSKAEEL
T558 NWRGMLKTSKAEELL
K560 RGMLKTSKAEELLAE
K569 EELLAEEKSKPIPIM
S570 ELLAEEKSKPIPIMP
K571 LLAEEKSKPIPIMPA
S579 PIPIMPASPQKGHAV
S600 VPVARKLSAREQRDC
S656 LVGQLYKSSLLDDLL
4494 : Phospho-DRP1 (Ser616) (D9A1) Rabbit mAb
  mouse

► Hide Isoforms
 
S71-p ILQLVHVsPEDKRKT
T78 sPEDKRKTTGEENGK
T79 PEDKRKTTGEENGKF
S88 EENGKFQSWRVEAEE
K166-u VPVGDQPkDIELQIR
K244-u MGRVIPVkLGIIGVV
K289 LANRNGTKYLARTLN
K311-u RDCLPELkTRINVLA
Y374 CGGARICYIFHETFG
T400 LNTIDILTAIRNATG
D509 TKHPDFADACGLMNN
S535-p RELPSAGsRDKSSKV
K538 PSAGsRDKSSKVPSA
K541 GsRDKSSKVPSALAP
S550 PSALAPASQEPPPAA
P554 APASQEPPPAASAEA
K564 ASAEADGKLIQDNRR
K574 QDNRRETKNVPSAGG
T592-p DGGQEPTtGNWRGML
K600 GNWRGMLKTSKAEEL
T601 NWRGMLKTSKAEELL
K603 RGMLKTSKAEELLAE
K612 EELLAEEKSKPIPIM
S613 ELLAEEKSKPIPIMP
K614 LLAEEKSKPIPIMPA
S622-p PIPIMPAsPQKGHAV
S643-p VPVARKLsAREQRDC
S699 LVGQLYKSSLLDDLL
3455 : Phospho-DRP1 (Ser616) Antibody
4494 : Phospho-DRP1 (Ser616) (D9A1) Rabbit mAb
4867 : Phospho-DRP1 (Ser637) Antibody
6319 : Phospho-DRP1 (Ser637) (D3A4) Rabbit mAb
  DRP1 iso3  
S71 ILQLVHVSPEDKRKT
T78 SPEDKRKTTGEENGV
T79 PEDKRKTTGEENGVE
- gap
K160 VPVGDQPKDIELQIR
K238 MGRVIPVKLGIIGVV
K283 LANRNGTKYLARTLN
K305 RDCLPELKTRINVLA
Y368 CGGARICYIFHETFG
T394 LNTIDILTAIRNATG
D503 TKHPDFADACGLMNN
S529 RELPSAGSRDKVPSA
K532 PSAGSRDKVPSAGGG
- gap
- gap
- gap
- gap
- gap
T549 DGGQEPTTGNWRGML
K557 GNWRGMLKTSKAEEL
T558 NWRGMLKTSKAEELL
K560 RGMLKTSKAEELLAE
K569 EELLAEEKSKPIPIM
S570 ELLAEEKSKPIPIMP
K571 LLAEEKSKPIPIMPA
S579-p PIPIMPAsPQKGHAV
S600-p VPVARKLsAREQRDC
S656 LVGQLYKSSLLDDLL
3455 : Phospho-DRP1 (Ser616) Antibody
4494 : Phospho-DRP1 (Ser616) (D9A1) Rabbit mAb
4867 : Phospho-DRP1 (Ser637) Antibody
6319 : Phospho-DRP1 (Ser637) (D3A4) Rabbit mAb
  DRP1 iso6  
S71 ILQLVHVSPEDKRKT
T78 SPEDKRKTTGEENDP
T79 PEDKRKTTGEENDPA
S95-p WKNSRHLsKGVEAEE
K173 VPVGDQPKDIELQIR
K251 MGRVIPVKLGIIGVV
K296 LANRNGTKYLARTLN
K318 RDCLPELKTRINVLA
Y381 CGGARICYIFHETFG
T407 LNTIDILTAIRNATG
D516 TKHPDFADACGLMNN
S542 RELPSAGSRDKVPSA
K545 PSAGSRDKVPSAGGG
- gap
- gap
- gap
- gap
- gap
T562 DGGQEPTTGNWRGML
K570 GNWRGMLKTSKAEEL
T571 NWRGMLKTSKAEELL
K573 RGMLKTSKAEELLAE
K582 EELLAEEKSKPIPIM
S583 ELLAEEKSKPIPIMP
K584 LLAEEKSKPIPIMPA
S592 PIPIMPASPQKGHAV
S613 VPVARKLSAREQRDC
S669 LVGQLYKSSLLDDLL
  rat

 
S71 ILQLVHVSPEDKRKT
T78 SPEDKRKTTGEENDP
T79 PEDKRKTTGEENDPA
S95 WKNSRHLSKGVEAEE
K173 VPVGDQPKDIELQIR
K251 MGRVIPVKLGIIGVV
K296 LANRNGTKYLARTLN
K318 RDCLPELKTRINVLA
Y381-p CGGARICyIFHETFG
T407 LNTIDILTAIRNATG
D516 TKHPDFADACGLMNN
S542 RELPSAVSRDKSSKV
K545 PSAVSRDKSSKVPSA
K548 VSRDKSSKVPSALAP
S557 PSALAPASQEPSPAA
S561 APASQEPSPAASAEA
K571 ASAEADGKLIQDNRR
K581 QDNRRETKNVASAGG
T605 DGGQEPTTGNWRGML
K613 GNWRGMLKTSKAEEL
T614 NWRGMLKTSKAEELL
K616 RGMLKTSKAEELLAE
K625 EELLAEEKSKPIPIM
S626 ELLAEEKSKPIPIMP
K627 LLAEEKSKPIPIMPA
S635-p PIPIMPAsPQKGHAV
S656-p VPVARKLsAREQRDC
S712 LVGQLYKSSLLDDLL
3455 : Phospho-DRP1 (Ser616) Antibody
4494 : Phospho-DRP1 (Ser616) (D9A1) Rabbit mAb
4867 : Phospho-DRP1 (Ser637) Antibody
6319 : Phospho-DRP1 (Ser637) (D3A4) Rabbit mAb
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