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Protein Page:
HMGB1 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
HMGB1 DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Belongs to the HMGB family. Note: This description may include information from UniProtKB.
Protein type: Nuclear receptor co-regulator; DNA repair, damage
Cellular Component: nucleoplasm; extracellular space; cell surface; transcriptional repressor complex; extracellular region; condensed chromosome; nucleus
Molecular Function: RAGE receptor binding; protein binding; double-stranded DNA binding; damaged DNA binding; cytokine activity; DNA bending activity; transcription factor activity; transcription factor binding; single-stranded DNA binding; chemoattractant activity
Biological Process: negative regulation of transcriptional preinitiation complex assembly; DNA topological change; V(D)J recombination; apoptosis; positive regulation of apoptosis; positive regulation of caspase activity; base-excision repair, DNA ligation; negative regulation of transcription from RNA polymerase II promoter; DNA recombination; regulation of transcription from RNA polymerase II promoter; myeloid dendritic cell activation; inflammatory response to antigenic stimulus; positive chemotaxis; dendritic cell chemotaxis; DNA ligation during DNA repair; innate immune response; positive regulation of transcription from RNA polymerase II promoter; DNA fragmentation during apoptosis; neurite development; positive regulation of DNA binding; cell structure disassembly during apoptosis
Reference #:  P09429 (UniProtKB)
Alt. Names/Synonyms: Amphoterin; DKFZp686A04236; high mobility group box 1; High mobility group protein 1; High mobility group protein B1; high-mobility group (nonhistone chromosomal) protein 1; high-mobility group box 1; HMG-1; HMG1; HMG3; HMGB1; SBP-1; Sulfoglucuronyl carbohydrate binding protein
Gene Symbols: HMGB1
Molecular weight: 24,894 Da
Basal Isoelectric point: 5.62  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

HMGB1

Protein Structure Not Found.


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Sites Implicated In
cell motility, altered: S35‑p, S39‑p, S42‑p
intracellular localization: S35‑p, S39‑p, S42‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
8 0 K3-a _____MGkGDPKKPR
3 6 K12-a DPKKPRGkMSSYAFF
0 2 K30-a CREEHKKkHPDAsVN
0 1 K30-u CREEHKKkHPDAsVN
2 12 S35-p KKkHPDAsVNFsEFs
3 4 S39-p PDAsVNFsEFskkCS
2 2 S42-p sVNFsEFskkCSERW
0 13 K43-a VNFsEFskkCSERWK
0 2 K43-u VNFsEFskkCSERWK
0 8 K44-a NFsEFskkCSERWKT
1 0 S53-p SERWKTMsAkEKGkF
0 10 K55-a RWKTMsAkEKGkFED
0 4 K59-a MsAkEKGkFEDMAKA
0 2 Y71-p AKADKARyEREMkty
0 1 K76-a ARyEREMktyIPPkG
0 2 T77-p RyEREMktyIPPkGE
0 2 Y78-p yEREMktyIPPkGET
5 2 K82-a MktyIPPkGETKKKF
0 13 S100-p NAPKRPPsAFFLFCS
0 24 Y109-p FFLFCSEyRPkIkGE
1 0 K112-m FCSEyRPkIkGEHPG
0 5 K112-u FCSEyRPkIkGEHPG
0 44 K114-u SEyRPkIkGEHPGLs
0 2 S121-p kGEHPGLsIGDVAkk
0 3 K127-u LsIGDVAkkLGEMWN
0 5 K128-u sIGDVAkkLGEMWNN
0 3 Y144-p AADDKQPyEkkAAKL
0 5 K146-u DDKQPyEkkAAKLKE
0 4 K147-u DKQPyEkkAAKLKEK
0 10 K157-a KLKEKYEkDIAAyRA
0 12 K157-u KLKEKYEkDIAAyRA
0 56 Y162-p YEkDIAAyRAKGKPD
0 2 K177-a AAKKGVVkAEKsKKK
2 0 S181-p GVVkAEKsKKKKEEE
  mouse

 
K3 _____MGKGDPKKPR
K12-a DPKKPRGkMSSYAFF
K30 CREEHKKKHPDAsVN
K30 CREEHKKKHPDAsVN
S35-p KKKHPDAsVNFsEFs
S39-p PDAsVNFsEFskKCS
S42-p sVNFsEFskKCSERW
K43-a VNFsEFskKCSERWK
K43-u VNFsEFskKCSERWK
K44 NFsEFskKCSERWKT
S53 SERWKTMSAkEKGkF
K55-a RWKTMSAkEKGkFED
K59-a MSAkEKGkFEDMAKA
Y71 AKADKARYEREMKTY
K76 ARYEREMKTYIPPKG
T77 RYEREMKTYIPPKGE
Y78 YEREMKTYIPPKGET
K82 MKTYIPPKGETKKKF
S100-p NAPKRPPsAFFLFCS
Y109 FFLFCSEYRPKIkGE
K112 FCSEYRPKIkGEHPG
K112 FCSEYRPKIkGEHPG
K114-u SEYRPKIkGEHPGLS
S121 kGEHPGLSIGDVAkk
K127-u LSIGDVAkkLGEMWN
K128-u SIGDVAkkLGEMWNN
Y144 AADDKQPYEkkAAKL
K146-u DDKQPYEkkAAKLKE
K147-u DKQPYEkkAAKLKEK
K157 KLKEKYEKDIAAyRA
K157-u KLKEKYEkDIAAyRA
Y162-p YEkDIAAyRAKGKPD
K177 AAKKGVVKAEKSKKK
S181 GVVKAEKSKKKKEEE
  rat

 
K3-a _____MGkGDPKKPR
K12 DPKKPRGKMSSYAFF
K30 CREEHKKKHPDASVN
K30 CREEHKKKHPDASVN
S35 KKKHPDASVNFSEFS
S39 PDASVNFSEFSKKCS
S42 SVNFSEFSKKCSERW
K43 VNFSEFSKKCSERWK
K43 VNFSEFSKKCSERWK
K44 NFSEFSKKCSERWKT
S53 SERWKTMSAKEKGKF
K55 RWKTMSAKEKGKFED
K59 MSAKEKGKFEDMAKA
Y71 AKADKARYEREMKTY
K76 ARYEREMKTYIPPkG
T77 RYEREMKTYIPPkGE
Y78 YEREMKTYIPPkGET
K82-a MKTYIPPkGETKKKF
S100 NAPKRPPSAFFLFCS
Y109 FFLFCSEYRPKIKGE
K112 FCSEYRPKIKGEHPG
K112 FCSEYRPKIKGEHPG
K114 SEYRPKIKGEHPGLS
S121 KGEHPGLSIGDVAKK
K127 LSIGDVAKKLGEMWN
K128 SIGDVAKKLGEMWNN
Y144 AADDKQPYEKKAAKL
K146 DDKQPYEKKAAKLKE
K147 DKQPYEKKAAKLKEK
K157 KLKEKYEKDIPAYRA
K157 KLKEKYEKDIPAYRA
Y162 YEKDIPAYRAKGKPD
K177 AAKKGVVKAEKsKKK
S181-p GVVKAEKsKKKKEEE
  cow

 
K3-a _____MGkGDPKKPR
K12-a DPKKPRGkMSSYAFF
K30 CREEHKKKHPDASVN
K30 CREEHKKKHPDASVN
S35 KKKHPDASVNFSEFS
S39 PDASVNFSEFSKKCS
S42 SVNFSEFSKKCSERW
K43 VNFSEFSKKCSERWK
K43 VNFSEFSKKCSERWK
K44 NFSEFSKKCSERWKT
S53 SERWKTMSAKEKGKF
K55 RWKTMSAKEKGKFED
K59 MSAKEKGKFEDMAKA
Y71 AKADKARYEREMKTY
K76 ARYEREMKTYIPPKG
T77 RYEREMKTYIPPKGE
Y78 YEREMKTYIPPKGET
K82 MKTYIPPKGETKKKF
S100 NAPKRPPSAFFLFCS
Y109 FFLFCSEYRPKIKGE
K112 FCSEYRPKIKGEHPG
K112 FCSEYRPKIKGEHPG
K114 SEYRPKIKGEHPGLS
S121 KGEHPGLSIGDVAKK
K127 LSIGDVAKKLGEMWN
K128 SIGDVAKKLGEMWNN
Y144 AADDKQPYEKKAAKL
K146 DDKQPYEKKAAKLKE
K147 DKQPYEKKAAKLKEK
K157 KLKEKYEKDIAAYRA
K157 KLKEKYEKDIAAYRA
Y162 YEKDIAAYRAKGKPD
K177 AAKKGVVKAEKSKKK
S181 GVVKAEKSKKKKEEE
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