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Protein Page:
GRP94 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
GRP94 Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with OS9. Interacts with CNPY3. This interaction is disrupted in the presence of ATP. Interacts with TLR4 and TLR9, but not with TLR3. Belongs to the heat shock protein 90 family. Note: This description may include information from UniProtKB.
Protein type: Secreted, signal peptide; Apoptosis; Heat shock protein; Secreted
Cellular Component: endoplasmic reticulum membrane; perinuclear region of cytoplasm; endoplasmic reticulum lumen; endoplasmic reticulum; melanosome; plasma membrane; extracellular region; midbody; cytosol; nucleus
Molecular Function: protein binding; low-density lipoprotein receptor binding; RNA binding; unfolded protein binding; virion binding; calcium ion binding; protein phosphatase binding; ATP binding
Biological Process: actin rod formation; ER-associated protein catabolic process; protein transport; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; protein folding; unfolded protein response; sequestering of calcium ion; response to hypoxia; toll-like receptor signaling pathway; innate immune response; regulation of phosphoprotein phosphatase activity; negative regulation of apoptosis
Reference #:  P14625 (UniProtKB)
Alt. Names/Synonyms: 94 kDa glucose-regulated protein; ECGP; Endoplasmin; endothelial cell (HBMEC) glycoprotein; ENPL; glucose regulated protein, 94 kDa; GP96; gp96 homolog; GRP-94; GRP94; Heat shock protein 90 kDa beta member 1; heat shock protein 90kDa beta (Grp94), member 1; HSP90B1; TRA1; tumor rejection antigen (gp96) 1; Tumor rejection antigen 1; Tumor rejection antigen-1 (gp96)
Gene Symbols: HSP90B1
Molecular weight: 92,469 Da
Basal Isoelectric point: 4.76  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

GRP94

Protein Structure Not Found.


STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB  |  Source  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene


Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 D26 VRADDEVDVdGTVEE
0 2 D28-ca ADDEVDVdGTVEEDL
0 2 S42-p LGKSREGsRtDDEVV
0 1 T44-p KSREGsRtDDEVVQR
0 1 D59 EEEAIQLDGLNASQI
0 6 K75-u ELREKSEkFAFQAEV
0 1 S92-p MMKLIINsLykNkEI
0 4 Y94-p KLIINsLykNkEIFL
0 8 K95-u LIINsLykNkEIFLR
0 4 K97-u INsLykNkEIFLREL
0 3 S106-p IFLRELIsNAsDALD
0 2 S109-p RELIsNAsDALDkIR
0 13 K114-u NAsDALDkIRLISLT
0 1 T133 LSGNEELTVKIKCDK
0 1 K142-u KIKCDKEkNLLHVTD
0 1 T148 EkNLLHVTDTGVGMT
0 1 T150 NLLHVTDTGVGMTRE
0 2 K161-u MTREELVkNLGTIAk
0 12 K168-u kNLGTIAkSGTSEFL
0 1 T179-p SEFLNKMtEAQEDGQ
0 1 Y200-p GQFGVGFysAFLVAD
0 1 S201-p QFGVGFysAFLVADK
0 1 T248 LGRGTTITLVLkEEA
0 2 K252-u TTITLVLkEEASDYL
0 4 K265-u YLELDTIkNLVKkYS
0 8 K270-u TIkNLVKkYSQFINF
0 14 S306-p AKEEKEEsDDEAAVE
1 0 S347-p KPIWQRPsKEVEEDE
0 5 K360-u DEYKAFYkSFSKESD
0 4 K404-a LFDEYGSkkSDYIkL
0 2 K404-u LFDEYGSkkSDYIkL
0 1 K405-u FDEYGSkkSDYIkLY
0 6 K410-u SkkSDYIkLYVRRVF
0 10 K434-u PKYLNFVkGVVDSDD
0 4 K455-u RETLQQHkLLKVIRK
0 6 K467-u IRKKLVRktLDMIkK
0 1 T468-p RKKLVRktLDMIkKI
0 2 K473 RktLDMIKKIADDKY
0 2 K473-u RktLDMIkKIADDKY
0 1 K486-u KYNDTFWkEFGTNIK
0 1 S552-p SRKEAESsPFVERLL
0 26 K586-a ALPEFDGkRFQNVAk
0 4 K586-u ALPEFDGkRFQNVAk
0 3 K593-u kRFQNVAkEGVKFDE
0 1 K597 NVAkEGVKFDESEKT
0 3 K613-a ESREAVEkEFEPLLN
0 2 K633-u ALKDKIEkAVVSQRL
0 13 Y652-p CALVASQyGWSGNME
0 5 K663-u GNMERIMkAQAYQTG
0 8 K671-u AQAYQTGkDISTNyy
0 63 Y677-p GkDISTNyyASQkKt
0 36 Y678-p kDISTNyyASQkKtF
0 8 K682-a TNyyASQkKtFEINP
0 5 K682-u TNyyASQkKtFEINP
0 2 T684-p yyASQkKtFEINPRH
0 2 K733-u GYLLPDTkAYGDRIE
0 1 T786-p DEEMDVGtDEEEETA
  mouse

 
D26-ca VRADDEVdVdGTVEE
D28-ca ADDEVdVdGTVEEDL
S42-p LGKSREGsRtDDEVV
T44-p KSREGsRtDDEVVQR
D59-ca EEEAIQLdGLNASQI
K75-u ELREKSEkFAFQAEV
S92 MMKLIINSLYkNKEI
Y94 KLIINSLYkNKEIFL
K95-u LIINSLYkNKEIFLR
K97 INSLYkNKEIFLREL
S106-p IFLRELIsNAsDALD
S109-p RELIsNAsDALDkIR
K114-u NAsDALDkIRLISLT
T133-p LAGNEELtVKIKCDK
K142 KIKCDKEKNLLHVtD
T148-p EKNLLHVtDtGVGMT
T150-p NLLHVtDtGVGMTRE
K161-u MTREELVkNLGTIAk
K168-u kNLGTIAkSGTSEFL
T179 SEFLNKMTEAQEDGQ
Y200 GQFGVGFYSAFLVAD
S201 QFGVGFYSAFLVADK
T248-p LGRGTTItLVLKEEA
K252 TTItLVLKEEASDYL
K265-u YLELDTIkNLVRKYS
K270 TIkNLVRKYSQFINF
S306-p AKEEKEEsDDEAAVE
S347 KPIWQRPSKEVEEDE
K360-u DEYKAFYkSFSKESD
K404-a LFDEYGSkKSDYIKL
K404-u LFDEYGSkKSDYIKL
K405 FDEYGSkKSDYIKLY
K410 SkKSDYIKLYVRRVF
K434-u PKYLNFVkGVVDSDD
K455-u RETLQQHkLLKVIRK
K467 IRKKLVRKTLDMIkK
T468 RKKLVRKTLDMIkKI
K473-a RKTLDMIkKIADEKY
K473 RKTLDMIKKIADEKY
K486 KYNDTFWKEFGTNIK
S552-p SRKEAESsPFVERLL
K586-a ALPEFDGkRFQNVAK
K586 ALPEFDGKRFQNVAK
K593 kRFQNVAKEGVkFDE
K597-a NVAKEGVkFDESEKT
K613 ESREATEKEFEPLLN
K633 ALKDKIEKAVVSQRL
Y652 CALVASQYGWSGNME
K663-u GNMERIMkAQAYQTG
K671-u AQAYQTGkDISTNyy
Y677-p GkDISTNyyASQkKt
Y678-p kDISTNyyASQkKtF
K682-a TNyyASQkKtFEINP
K682-u TNyyASQkKtFEINP
T684-p yyASQkKtFEINPRH
K733 GYLLPDTKAYGDRIE
T785-p GEEMDAGtEEEEEET
  rat

 
D26 VRADDEVDVDGTVEE
D28 ADDEVDVDGTVEEDL
S42 LGKSREGSRTDDEVV
T44 KSREGSRTDDEVVQR
D59 EEEAIQLDGLNASQI
K75 ELREKSEKFAFQAEV
S92 MMKLIINSLYKNKEI
Y94 KLIINSLYKNKEIFL
K95 LIINSLYKNKEIFLR
K97 INSLYKNKEIFLREL
S106 IFLRELISNASDALD
S109 RELISNASDALDKIR
K114 NASDALDKIRLISLT
T133 LAGNEELTVKIKCDR
K142 KIKCDREKNLLHVTD
T148 EKNLLHVTDTGVGMT
T150 NLLHVTDTGVGMTRE
K161 MTREELVKNLGTIAK
K168 KNLGTIAKSGTSEFL
T179 SEFLNKMTEAQEDGQ
Y200 GQFGVGFYSAFLVAD
S201 QFGVGFYSAFLVADK
T248 LGRGTTITLVLKEEA
K252 TTITLVLKEEASDYL
K265 YLELDTIKNLVRKYS
K270 TIKNLVRKYSQFINF
A306 AQEEKEEADDEAAVE
S347 KPIWQRPSKEVEEDE
K360 DEYKAFYKSFSKESD
K404 LFDEYGSKKSDYIKL
K404 LFDEYGSKKSDYIKL
K405 FDEYGSKKSDYIKLY
K410 SKKSDYIKLYVRRVF
K434 PKYLNFVKGVVDSDD
K455 RETLQQHKLLKVIRK
K467 IRKKLVRKTLDMIKK
T468 RKKLVRKTLDMIKKI
K473 RKTLDMIKKIADEKY
K473 RKTLDMIKKIADEKY
K486 KYNDTFWKEFGTNIK
S552 SRKEAESSPFVERLL
K586 ALPEFDGKRFQNVAK
K586 ALPEFDGKRFQNVAK
K593 KRFQNVAKEGVKFDE
K597 NVAKEGVKFDESEKS
K613 ESREATEKEFEPLLN
K633 ALKDKIEKAVVSQRL
Y652 CALVASQYGWSGNME
K663 GNMERIMKAQAYQTG
K671 AQAYQTGKDISTNYY
Y677 GKDISTNYYASQKKT
Y678 KDISTNYYASQKKTF
K682 TNYYASQKKTFEINP
K682 TNYYASQKKTFEINP
T684 YYASQKKTFEINPRH
K733 GYLLPDTKAYGDRIE
- gap
  dog

 
D26 VRADDEVDVDGTVEE
D28 ADDEVDVDGTVEEDL
S42 LGKSREGSRTDDEVV
T44 KSREGSRTDDEVVQR
D59 EEEAIQLDGLNASQI
K75 ELREKSEKFAFQAEV
S92 MMKLIINSLYKNKEI
Y94 KLIINSLYKNKEIFL
K95 LIINSLYKNKEIFLR
K97 INSLYKNKEIFLREL
S106 IFLRELISNASDALD
S109 RELISNASDALDKIR
K114 NASDALDKIRLISLT
T133 LAGNEELTVKIKCDK
K142 KIKCDKEKNLLHVTD
T148 EKNLLHVTDTGVGMT
T150 NLLHVTDTGVGMTRE
K161 MTREELVKNLGTIAK
K168 KNLGTIAKSGTSEFL
T179 SEFLNKMTEAQEDGQ
Y200 GQFGVGFYSAFLVAD
S201 QFGVGFYSAFLVADK
T248 LGRGTTITLVLKEEA
K252 TTITLVLKEEASDYL
K265 YLELDTIKNLVKKYS
K270 TIKNLVKKYSQFINF
S306 AKEEKEDSDDEAAVE
S347 KPIWQRPSKEVEDDE
K360 DEYKAFYKSFSKESD
K404 LFDEYGSKKSDYIKL
K404 LFDEYGSKKSDYIKL
K405 FDEYGSKKSDYIKLY
K410 SKKSDYIKLYVRRVF
K434 PKYLNFVKGVVDSDD
K455 RETLQQHKLLKVIRK
K467 IRKKLVRKTLDMIKK
T468 RKKLVRKTLDMIKKI
K473 RKTLDMIKKIADEKY
K473 RKTLDMIKKIADEKY
K486 KYNDTFWKEFGTNIK
S552 SRKEAESSPFVERLL
K586 ALPEFDGKRFQNVAK
K586 ALPEFDGKRFQNVAK
K593 KRFQNVAKEGVKFDE
K597 NVAKEGVKFDESEKT
K613 ESREAIEKEFEPLLN
K633 ALKDKIEKAVVSQRL
Y652 CALVASQYGWSGNME
K663 GNMERIMKAQAYQTG
K671 AQAYQTGKDISTNYY
Y677 GKDISTNYYASQKKT
Y678 KDISTNYYASQKKTF
K682 TNYYASQKKTFEINP
K682 TNYYASQKKTFEINP
T684 YYASQKKTFEINPRH
K733 GYLLPDTKAYGDRIE
T786 EEEMDAGTDDEEQET
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