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Protein Page:
CHD-8 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
CHD-8 DNA helicase that acts as a chromatin remodeling factor and regulates transcription. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1- targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating in efficient U6 RNA polymerase III transcription. Interacts with p53/TP53, histone H1, CTNNB1, CTCF and PIAS3. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, KAT8/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with CHD7. Belongs to the SNF2/RAD54 helicase family. CHD8 subfamily. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: EC 3.6.1.-; Helicase; EC 3.6.4.12; Transcription factor
Cellular Component: protein complex; nucleus
Molecular Function: DNA-dependent ATPase activity; protein binding; DNA helicase activity; DNA binding; histone binding; p53 binding; beta-catenin binding; ATP binding; methylated histone residue binding
Biological Process: ATP catabolic process; negative regulation of Wnt receptor signaling pathway; transcription, DNA-dependent; in utero embryonic development; positive regulation of transcription, DNA-dependent; ATP-dependent chromatin remodeling; positive regulation of transcription from RNA polymerase III promoter; positive regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; negative regulation of transcription, DNA-dependent; DNA duplex unwinding
Reference #:  Q9HCK8 (UniProtKB)
Alt. Names/Synonyms: ATP-dependent helicase CHD8; axis duplication inhibitor; beta-catenin binding protein-like; CHD-8; CHD8; chromodomain helicase DNA binding protein 8; Chromodomain-helicase-DNA-binding protein 8; DKFZp686N17164; duplin; Helicase with SNF2 domain 1; HELSNF1; KIAA1564
Gene Symbols: CHD8
Molecular weight: 290,519 Da
Basal Isoelectric point: 6.04  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

CHD-8

Protein Structure Not Found.


STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho3D  |  InnateDB  |  UCSD-Nature  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene


Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 S125 GLLQVSKSQEILSQG
0 1 P153 SSAGGQPPQSAPKIV
0 1 S155 AGGQPPQSAPKIVIL
0 1 S256 VLQPVKGSAPAGNPG
0 1 K270-u GATGPPLkPAVTLTS
0 1 T274 PPLkPAVTLTStPTQ
0 3 T278-p PAVTLTStPTQGESK
0 3 S432-p SEVAALSsPASSAPH
0 1 S519 KEEKPKKSKTSGASK
0 1 T521 EKPKKSKTSGASKtK
0 1 S525 KSKTSGASKtKGKsK
0 1 T527-p KTSGASKtKGKsKLN
0 1 S531-p ASKtKGKsKLNtItP
0 1 T535-p KGKsKLNtItPVVGK
0 2 T537-p KsKLNtItPVVGKKR
0 2 S549-p KKRKRNTssDNsDVE
0 4 S550-p KRKRNTssDNsDVEV
0 7 S553-p RNTssDNsDVEVMPA
0 4 S562-p VEVMPAQsPREDEES
1 0 K592-s YTEDLDIkITDDEEE
0 1 K1301-a GIQQFSKkEIEDLLR
0 13 T1398-p KNNLVIDtPRVRKQT
0 29 S1420-p DDDLVEFsDLEsEDD
0 31 S1424-p VEFsDLEsEDDERPR
0 3 S1466-p GRWRDILsHGRFKRR
0 1 S1679-p HRVLDNFsDIVEGVD
0 1 Y1696 KDCEDPEYKPLQGPP
0 1 S1842-p LDKKTDEsLTKYFHG
0 8 S1976-p QAGAPAPsLsRCstP
0 23 S1978-p GAPAPsLsRCstPLL
0 9 S1981-p APsLsRCstPLLHQQ
0 37 T1982-p PsLsRCstPLLHQQY
0 1 T1990-p PLLHQQYtsRtAsPL
0 2 S1991-p LLHQQYtsRtAsPLP
0 8 T1993-p HQQYtsRtAsPLPLR
0 12 S1995-p QYtsRtAsPLPLRPD
0 8 - gap
0 16 S2008-p PDAPVEKsPEEtAtQ
0 3 T2012-p VEKsPEEtAtQVPSL
0 1 T2014-p KsPEEtAtQVPSLES
0 1 T2037-p VVARSRPtPQDYEMR
0 36 - gap
0 17 S2046-p QDYEMRVsPSDTtPL
0 6 T2051-p RVsPSDTtPLVSRSV
0 5 S2069-p KLEDEDDsDsELDLS
0 5 S2071-p EDEDDsDsELDLSKL
0 2 S2126-p SLLSLTMsQDGFPNE
0 2 S2182-p KWPSSRRsQEMVTGG
0 9 S2200-p PGNHLLDsPsLtPGE
0 8 S2202-p NHLLDsPsLtPGEYG
0 6 T2204-p LLDsPsLtPGEYGDs
0 15 S2211-p tPGEYGDsPVPtPRS
0 7 T2215-p YGDsPVPtPRSSSAA
0 2 S2218 sPVPtPRSSSAAsMA
0 2 S2220 VPtPRSSSAAsMAEE
0 1 S2223-p PRSSSAAsMAEEEAS
0 1 S2389 EPVQTANSRNGKKGH
0 17 Y2516-p PGLRAPGyPssPVTT
0 29 S2518-p LRAPGyPssPVTTAS
0 118 S2519-p RAPGyPssPVTTASG
0 1 S2551-p EEDDDDLsQGYDsSE
0 1 S2556-p DLsQGYDsSERDFSL
0 1 S2573 DPMMPANSDSSEDAD
0 1 S2575 MMPANSDSSEDADD_
0 1 S2576 MPANSDSSEDADD__
  CHD-8 iso2  
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S153 SEVAALSSPASSAPH
S240 KEEKPKKSKTSGASK
T242 EKPKKSKTSGASKTK
S246 KSKTSGASKTKGKSK
T248 KTSGASKTKGKSKLN
S252 ASKTKGKSKLNTITP
T256 KGKSKLNTITPVVGK
T258 KSKLNTITPVVGKKR
S270 KKRKRNTSSDNSDVE
S271 KRKRNTSSDNSDVEV
S274 RNTSSDNSDVEVMPA
S283 VEVMPAQSPREDEES
K313 YTEDLDIKITDDEEE
K1022 GIQQFSKKEIEDLLR
T1119 KNNLVIDTPRVRKQT
S1141 DDDLVEFSDLESEDD
S1145 VEFSDLESEDDERPR
S1187 GRWRDILSHGRFKRR
S1400 HRVLDNFSDIVEGVD
Y1417 KDCEDPEYKPLQGPP
S1563 LDKKTDESLTKYFHG
S1697 QAGAPAPSLSRCSTP
S1699 GAPAPSLSRCSTPLL
S1702 APSLSRCSTPLLHQQ
T1703 PSLSRCSTPLLHQQY
T1711 PLLHQQYTSRTASPL
S1712 LLHQQYTSRTASPLP
T1714 HQQYTSRTASPLPLR
S1716 QYTSRTASPLPLRPD
- gap
S1729 PDAPVEKSPEETATQ
T1733 VEKSPEETATQVPSL
T1735 KSPEETATQVPSLES
T1758 VVARSRPTPQDYEMR
- gap
S1767 QDYEMRVSPSDTTPL
T1772 RVSPSDTTPLVSRSV
S1790 KLEDEDDSDSELDLS
S1792 EDEDDSDSELDLSKL
S1847 SLLSLTMSQDGFPNE
S1903 KWPSSRRSQEMVTGG
S1921 PGNHLLDSPSLTPGE
S1923 NHLLDSPSLTPGEYG
T1925 LLDSPSLTPGEYGDS
S1932 TPGEYGDSPVPTPRS
T1936 YGDSPVPTPRSSSAA
S1939 SPVPTPRSSSAASMA
S1941 VPTPRSSSAASMAEE
S1944 PRSSSAASMAEEEAS
S2110 EPVQTANSRNGKKGH
Y2237 PGLRAPGYPssPVTT
S2239-p LRAPGYPssPVTTAS
S2240-p RAPGYPssPVTTASG
S2272 EEDDDDLSQGYDSSE
S2277 DLSQGYDSSERDFSL
S2294 DPMMPANSDSSEDAD
S2296 MMPANSDSSEDADD_
S2297 MPANSDSSEDADD__
  mouse

► Hide Isoforms
 
S125-p GLLQVSKsQEILSQG
S153-p SNTGGQPsQsAPKIV
S155-p TGGQPsQsAPKIVIL
S256-p VLQPVKGsAPAGNPG
K270 GAAGPPLKPAVtLTS
T274-p PPLKPAVtLTSTPTQ
T278 PAVtLTSTPTQGESK
S434 SEVAALSSPASCAPH
S521-p KEEKPKKsKtAAAsK
T523-p EKPKKsKtAAAsKTK
S527-p KsKtAAAsKTKGKSK
T529 KtAAAsKTKGKSKLN
S533 AsKTKGKSKLNTITP
T537 KGKSKLNTITPVVGK
T539 KSKLNTITPVVGKKR
S551-p KKRKRNTssDNsDVE
S552-p KRKRNTssDNsDVEV
S555-p RNTssDNsDVEVMPA
S564-p VEVMPAQsPREDEES
K594 YTEDLDIKITDDEEE
K1303 GIQQFSKKEIEDLLR
T1400 KNNLVIDTPRVRKQT
S1422-p DDDLVEFsDLEsEDD
S1426-p VEFsDLEsEDDERPR
S1468-p GRWRDILsHGRFKRR
S1681-p HRVLDNFsDLVEGID
Y1698-p KDCEDPEyKPLQGPP
S1844 LDKKTDESLTKYFHG
S1978 QAGASAASLsRCSTP
S1980-p GASAASLsRCSTPLL
S1983 AASLsRCSTPLLHQQ
T1984 ASLsRCSTPLLHQQC
T1992 PLLHQQCTsRtAsPs
S1993-p LLHQQCTsRtAsPsP
T1995-p HQQCTsRtAsPsPLR
S1997-p QCTsRtAsPsPLRPD
S1999-p TsRtAsPsPLRPDAP
S2010-p PDAPVEKsPEESTVQ
S2014 VEKsPEESTVQVPNL
V2016 KsPEESTVQVPNLES
T2039-p VVARSRLtsQDYEVR
S2040-p VARSRLtsQDYEVRV
- gap
- gap
S2070-p KLEDEDDsDsELDLS
S2072-p EDEDDsDsELDLSKL
S2128 SLLSLTMSQDGFPNE
S2184 KWPSNRRSQEVTAGG
S2202-p PGNHLLDsPsLtPGE
S2204-p NHLLDsPsLtPGEDG
T2206-p LLDsPsLtPGEDGDs
S2213-p tPGEDGDsPVPtPRs
T2217-p DGDsPVPtPRsGsAA
S2220-p sPVPtPRsGsAAsMA
S2222-p VPtPRsGsAAsMAEE
S2225-p PRsGsAAsMAEEEAS
S2391-p EPVQPANsRNGKKGH
Y2517 PGLRTTGYPSsPATT
S2519 LRTTGYPSsPATTTS
S2520-p RTTGYPSsPATTTSG
S2552 DEEDDDLSQGYDSSE
S2557 DLSQGYDSSERDFSL
S2574-p DPMMPANsDssEDAD
S2576-p MMPANsDssEDADD_
S2577-p MPANsDssEDADD__
  CHD-8 iso2  
S125 GLLQVSKSQEILSQG
S153 SNTGGQPSQSAPKIV
S155 TGGQPSQSAPKIVIL
S256 VLQPVKGSAPAGNPG
K270 GAAGPPLKPAVTLTS
T274 PPLKPAVTLTSTPTQ
T278 PAVTLTSTPTQGESK
S434 SEVAALSSPASCAPH
S521 KEEKPKKSKTAAASK
T523 EKPKKSKTAAASKTK
S527 KSKTAAASKTKGKSK
T529 KTAAASKTKGKSKLN
S533 ASKTKGKSKLNTITP
T537 KGKSKLNTITPVVGK
T539 KSKLNTITPVVGKKR
S551 KKRKRNTSSDNSDVE
S552 KRKRNTSSDNSDVEV
S555 RNTSSDNSDVEVMPA
S564 VEVMPAQSPREDEES
K594 YTEDLDIKITDDEEE
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
  rat

 
S125 GLLQVSKSQEILSQG
S153 SNTGGQPSQSAPKIV
S155 TGGQPSQSAPKIVIL
S256 VLQPVKGSAPAGNPG
K270 GATGPPLKPAVTLTS
T274 PPLKPAVTLTSTPAQ
T278 PAVTLTSTPAQGESK
S432 SEVAALSSPASCAPH
S519 KEEKPKKSKTAAASK
T521 EKPKKSKTAAASKTK
S525 KSKTAAASKTKGKSK
T527 KTAAASKTKGKSKLN
S531 ASKTKGKSKLNTITP
T535 KGKSKLNTITPVVGK
T537 KSKLNTITPVVGKKR
S549 KKRKRNTSSDNSDVE
S550 KRKRNTSSDNSDVEV
S553 RNTSSDNSDVEVMPA
S562 VEVMPAQSPREDEES
K592 YTEDLDIKITDDEEE
K1301 GIQQFSKKEIEDLLR
T1398 KNNLVIDTPRVRKQT
S1420 DDDLVEFSDLEsEDD
S1424-p VEFSDLEsEDDERPR
S1466 GRWRDILSHGRFKRR
S1679 HRVLDNFSDLVEGID
Y1696 KDCEDPEYKPLQGPP
S1842 LDKKTDESLTKYFHG
S1976 QAGASAASLSRCSTP
S1978 GASAASLSRCSTPLL
S1981 AASLSRCSTPLLHQQ
T1982 ASLSRCSTPLLHQQC
T1990 PLLHQQCTSRTASPs
S1991 LLHQQCTSRTASPsP
T1993 HQQCTSRTASPsPLR
S1995 QCTSRTASPsPLRPD
S1997-p TSRTASPsPLRPDVP
S2008 PDVPAEKSPEENAVQ
N2012 AEKSPEENAVQVPSL
V2014 KSPEENAVQVPSLDS
T2037 VVARSRLTPQDYEIR
- gap
- gap
- gap
S2068 KLEDDDDSDSELDLS
S2070 EDDDDSDSELDLSKL
S2126 SLLSLTMSQDGFPNE
S2182 KWPSNRRSQEMTTGG
S2200 PGNHLLDSPSLTPGE
S2202 NHLLDSPSLTPGEYG
T2204 LLDSPSLTPGEYGDS
S2211 TPGEYGDSPVPTPRS
T2215 YGDSPVPTPRSSSAA
S2218 SPVPTPRSSSAASMV
S2220 VPTPRSSSAASMVEE
S2223 PRSSSAASMVEEEAS
S2389 EPVQPANSRNGKKGH
Y2516 PGLRTTGYPSSPATT
S2518 LRTTGYPSSPATTTS
S2519 RTTGYPSSPATTTSG
S2551 DEDDDDLSQGYDSSE
S2556 DLSQGYDSSERDFSL
S2573 DPMMPANSDSSDDAD
S2575 MMPANSDSSDDADD_
S2576 MPANSDSSDDADD__
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