Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Protein Page:
EHMT2 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
g O-GlcNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
EHMT2 Histone methyltransferase. Preferentially methylates 'Lys-9' of histone H3 and 'Lys-27' of histone H3 (in vitro). H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. Also methylates histone H1. Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with GFI1B, WIZ and EHMT1. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Methyltransferase, protein lysine; Methyltransferase; EC 2.1.1.-; Amino Acid Metabolism - lysine degradation; EC 2.1.1.43
Cellular Component: chromosome; nucleus
Molecular Function: protein binding; zinc ion binding; p53 binding; histone lysine N-methyltransferase activity (H3-K9 specific); protein-lysine N-methyltransferase activity; histone-lysine N-methyltransferase activity; histone lysine N-methyltransferase activity (H3-K27 specific)
Biological Process: histone methylation; synaptonemal complex assembly; organ growth; cytosine methylation within a CG sequence; fertilization; peptidyl-lysine di-methylation; DNA methylation; negative regulation of transcription from RNA polymerase II promoter; spermatid development; pachytene; regulation of DNA replication
Reference #:  A2ABF8 (UniProtKB)
Alt. Names/Synonyms: ankyrin repeat-containing protein; BAT8; C6orf30; DKFZp686H08213; EHMT2; Euchromatic histone-lysine N-methyltransferase 2; FLJ35547; G9A; G9A histone methyltransferase; H3-K9-HMTase 3; Histone H3-K9 methyltransferase 3; Histone-lysine N-methyltransferase, H3 lysine-9 specific 3; HLA-B associated transcript 8; HLA-B-associated transcript 8; KMT1C; Lysine N-methyltransferase 1C; NG36; Protein G9a
Gene Symbols: EHMT2
Molecular weight: 135,418 Da
Basal Isoelectric point: 5.81  Predict pI for various phosphorylation states
CST Pathways:  Histone Methylation
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

EHMT2
Protein Structure Not Found.


STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho3D  |  DISEASE  |  Source  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene  |  Ensembl Protein


Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


  MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


        human

► Hide Isoforms 		 
0 2 S41-p RGRGRPRsLLsLPRA
0 2 S44-p GRPRsLLsLPRAQAS
0 4 G96 GATERVHGsLGDtPR
0 3 S97-p ATERVHGsLGDtPRS
0 9 T101-p VHGsLGDtPRSEETL
0 1 S123 LEPAGPSSPASVTVT
0 1 S126 AGPSSPASVTVTVGD
1 1 K171-m2 ILLGHATkSFPssPs
1 0 K171 ILLGHATKSFPssPs
0 30 S175-p HATkSFPssPskGGs
0 58 S176-p ATkSFPssPskGGsC
0 1 S178-p kSFPssPskGGsCPS
0 1 K179-a SFPssPskGGsCPSR
0 2 S182-p ssPskGGsCPSRAKM
0 3 S190-p CPSRAKMsMTGAGKs
0 2 T192 SRAKMsMTGAGKsPP
0 11 S197-p sMTGAGKsPPSVQSL
0 1 S200 GAGKsPPSVQSLAMR
0 4 S210-p SLAMRLLsMPGAQGA
0 1 T228-p GSEPPPAtTsPEGQP
0 3 S230-p EPPPAtTsPEGQPKV
0 1 K242-m2 PKVHRARkTMSkPGN
1 3 K242-m3 PKVHRARkTMSkPGN
0 1 K246-m3 RARkTMSkPGNGQPP
0 1 S268-p EIQHFRMsDDVHSLG
0 12 S289-p AKRRKLNsGGGLSEE
0 2 G292 RKLNsGGGLSEELGS
0 2 S294 LNsGGGLSEELGSAR
0 1 S299 GLSEELGSARRsGEV
0 5 S303-p ELGSARRsGEVTLtK
0 1 T309-p RsGEVTLtKGDPGSL
0 1 K405-u KAKKKWRkDsPWVKP
0 1 S407-p KKKWRkDsPWVKPSR
0 3 S435-p RGVSNDTssLETERG
0 7 S436-p GVSNDTssLETERGF
0 4 T439 NDTssLETERGFEEL
0 1 T491-p AAILKREtMRPSSRV
0 13 T578-p IPRGDGVtPPAGtAA
0 2 T583-p GVtPPAGtAAPAPPP
0 25 S592-p APAPPPLsQDVPGRA
0 1 S605 RADTSQPSARMRGHG
0 1 S664-p KALVIQEsERRKKLR
0 1 - gap
0 1 - gap
0 1 Y768 VQRGGCVYSKEEDGS
0 1 K905-u ANPELRNkEGDTAWD
0 1 T909 LRNkEGDTAWDLTPE
0 2 K1070-u LQLYRTAkMGWGVRA
0 1 Y1108-p DVREDDSyLFDLDNK
0 1 S1131 ARYYGNISRFINHLC
0 1 T1169 FSSRDIRTGEELGFD
0 1 S1227-p ELLPELGsLPPVNt_
0 1 T1233-p GsLPPVNt_______
  EHMT2 iso2  
- gap
- gap
G39 GATERVHGSLGDtPR
S40 ATERVHGSLGDtPRS
T44-p VHGSLGDtPRSEETL
S66 LEPAGPSSPASVTVT
S69 AGPSSPASVTVTVGD
K114 ILLGHATKSFPSsPS
K114 ILLGHATKSFPSsPS
S118 HATKSFPSsPSKGGS
S119-p ATKSFPSsPSKGGSC
S121 KSFPSsPSKGGSCPS
K122 SFPSsPSKGGSCPSR
S125 SsPSKGGSCPSRAKM
S133 CPSRAKMSMTGAGKS
T135 SRAKMSMTGAGKSPP
S140 SMTGAGKSPPSVQSL
S143 GAGKSPPSVQSLAMR
S153 SLAMRLLSMPGAQGA
T171 GSEPPPATTSPEGQP
S173 EPPPATTSPEGQPKV
K185 PKVHRARKTMSKPGN
K185 PKVHRARKTMSKPGN
K189 RARKTMSKPGNGQPP
S211 EIQHFRMSDDVHSLG
S232 AKRRKLNSGGGLSEE
G235 RKLNSGGGLSEELGS
S237 LNSGGGLSEELGSAR
S242 GLSEELGSARRSGEV
S246 ELGSARRSGEVTLTK
T252 RSGEVTLTKGDPGSL
K348 KAKKKWRKDSPWVKP
S350 KKKWRKDSPWVKPSR
S378 RGVSNDTSSLETERG
S379 GVSNDTSSLETERGF
T382 NDTSSLETERGFEEL
T434 AAILKRETMRPSSRV
T521 IPRGDGVTPPAGTAA
T526 GVTPPAGTAAPAPPP
S535-p APAPPPLsQDVPGRA
S548 RADTSQPSARMRGHG
S607 KALVIQESERRKKLR
- gap
- gap
Y711 VQRGGCVYSKEEDGS
K848 ANPELRNKEGDTAWD
T852 LRNKEGDTAWDLTPE
K1013 LQLYRTAKMGWGVRA
Y1051 DVREDDSYLFDLDNK
S1074 ARYYGNISRFINHLC
T1112 FSSRDIRTGEELGFD
S1170 ELLPELGSLPPVNT_
T1176 GSLPPVNT_______
  mouse

► Hide Isoforms 		 
S41-p RGRGRPRsLLsLPRA
S44-p GRPRsLLsLPRAQAS
S96-p GAAERVHsSLGDTPQ
S97 AAERVHsSLGDTPQS
T101 VHsSLGDTPQSEETL
S123-p LEPAGPSsPAsVTVT
S126-p AGPSsPAsVTVTVGD
K167-m2 IVLGHATkSFPSsPS
K167-m3 IVLGHATkSFPSsPS
S171 HATkSFPSsPSKGGA
S172-p ATkSFPSsPSKGGAC
S174 kSFPSsPSKGGACPS
K175 SFPSsPSKGGACPSR
A178 SsPSKGGACPSRAKM
S186-p CPSRAKMsMtGAGKs
T188-p SRAKMsMtGAGKsPP
S193-p sMtGAGKsPPsVQSL
S196-p GAGKsPPsVQSLAMR
S206 SLAMRLLSMPGAQGA
T224 GPEPSPATTAAQEGQ
A226 EPSPATTAAQEGQPK
K239 PKVHRARKTMSKPSN
K239-m3 PKVHRARkTMSKPSN
K243 RARkTMSKPSNGQPP
S265 EVQHFRMSDDMHLGK
S285-p AKRRKLNsGsLsEDL
S287-p RRKLNsGsLsEDLGs
S289-p KLNsGsLsEDLGsAG
S294-p sLsEDLGsAGGsGDI
S298-p DLGsAGGsGDIILEK
E304 GsGDIILEKGEPRPL
K401 KAKKKWRKDSPWVKP
S403 KKKWRKDSPWVKPSR
S465-p AGVSNDTssLEtERG
S466-p GVSNDTssLEtERGF
T469-p NDTssLEtERGFEEL
T521 AAILKRETMRPSSRV
T608-p IPRGDGGtPPIGTAA
T613 GGtPPIGTAAPALPP
A622 APALPPLAHDAPGRA
S635-p RADTSQPsARMRGHG
S694 KALVIQESERRKKLR
- gap
- gap
Y798-p VQLGGCVySKEEDGS
K935 ANPELRNKEGDtAWD
T939-p LRNKEGDtAWDLTPE
K1100-u LQLYRTAkMGWGVRA
Y1138 DVREDDSYLFDLDNK
S1161-p ARYYGNIsRFINHLC
T1199-p FSSRDIRtGEELGFD
S1257 ELLPDLSSLPPINT_
T1263 SSLPPINT_______
  EHMT2 iso4  
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
T32 AAILKRETMRPSSRV
T119 IPRGDGGTPPIGTAA
T124 GGTPPIGTAAPALPP
A133 APALPPLAHDAPGRA
S146 RADTSQPSARMRGHG
S205 KALVIQESESPPsPP
S210-p QESESPPsPPPsPDR
S214-p SPPsPPPsPDRRKKL
Y319 VQLGGCVYSKEEDGS
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.