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Protein Page:
EHMT2 iso4 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
EHMT2 iso4 Histone methyltransferase. Preferentially methylates 'Lys-9' of histone H3 and 'Lys-27' of histone H3 (in vitro). H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. Also methylates histone H1. Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with GFI1B, WIZ and EHMT1. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Amino Acid Metabolism - lysine degradation; Methyltransferase, protein lysine; EC 2.1.1.-; EC 2.1.1.43; Methyltransferase
Cellular Component: nucleoplasm; chromosome; nucleus
Molecular Function: protein binding; zinc ion binding; p53 binding; histone lysine N-methyltransferase activity (H3-K9 specific); protein-lysine N-methyltransferase activity; histone-lysine N-methyltransferase activity; histone lysine N-methyltransferase activity (H3-K27 specific)
Biological Process: histone methylation; organ growth; synaptonemal complex assembly; cytosine methylation within a CG sequence; fertilization; peptidyl-lysine di-methylation; DNA methylation; negative regulation of transcription from RNA polymerase II promoter; spermatid development; regulation of DNA replication; histone H3-K9 methylation
Reference #:  A2ABF8 (UniProtKB)
Alt. Names/Synonyms: ankyrin repeat-containing protein; BAT8; C6orf30; DKFZp686H08213; EHMT2; Euchromatic histone-lysine N-methyltransferase 2; FLJ35547; G9A; G9A histone methyltransferase; H3-K9-HMTase 3; Histone H3-K9 methyltransferase 3; Histone-lysine N-methyltransferase, H3 lysine-9 specific 3; HLA-B associated transcript 8; HLA-B-associated transcript 8; KMT1C; Lysine N-methyltransferase 1C; NG36; Protein G9a
Gene Symbols: EHMT2
Molecular weight: 135,418 Da
Basal Isoelectric point: 5.81  Predict pI for various phosphorylation states
CST Pathways:  Histone Methylation
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

EHMT2 iso4

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 - gap
0 2 - gap
0 2 - gap
0 4 G39 GATERVHGsLGDtPR
0 6 S40-p ATERVHGsLGDtPRs
0 11 T44-p VHGsLGDtPRsEEtL
0 2 S47-p sLGDtPRsEEtLPKA
0 1 T50-p DtPRsEEtLPKATPD
0 1 S66 LEPAGPSSPASVTVT
0 1 S69 AGPSSPASVTVTVGD
0 1 P87 DTPVGATPLIGDESE
1 1 K114 ILLGHATKSFPSsPS
1 0 K114 ILLGHATKSFPSsPS
0 28 S118 HATKSFPSsPSKGGS
0 56 S119-p ATKSFPSsPSKGGSC
0 1 S121 KSFPSsPSKGGSCPS
0 1 K122 SFPSsPSKGGSCPSR
0 2 S125 SsPSKGGSCPSRAKM
0 4 S133 CPSRAKMSMtGAGKs
0 3 T135-p SRAKMSMtGAGKsPP
0 16 S140-p SMtGAGKsPPsVQSL
0 2 S143-p GAGKsPPsVQSLAMR
0 4 S153 SLAMRLLSMPGAQGA
0 1 T171 GSEPPPATTSPEGQP
0 3 S173 EPPPATTSPEGQPKV
0 1 K185 PKVHRARKTMSKPGN
1 3 K185 PKVHRARKTMSKPGN
0 1 K189 RARKTMSKPGNGQPP
0 1 S211 EIQHFRMSDDVHSLG
0 15 S232-p AKRRKLNsGGGLSEE
0 3 G235 RKLNsGGGLSEELGs
0 4 S237 LNsGGGLSEELGsAR
0 2 S242-p GLSEELGsARRsGEV
0 6 S246-p ELGsARRsGEVTLTK
0 1 T252 RsGEVTLTKGDPGSL
0 1 K348 KAKKKWRKDsPWVKP
0 3 S350-p KKKWRKDsPWVKPsR
0 1 S356-p DsPWVKPsRKRRKRE
0 3 S412 AGVSNDTSSLETERG
0 8 S413 GVSNDTSSLETERGF
0 4 T416 NDTSSLETERGFEEL
0 1 T468 AAILKRETMRPSSRV
0 17 T555-p IPRGDGVtPPAGtAA
0 4 T560-p GVtPPAGtAAPAPPP
0 27 S569-p APAPPPLsQDVPGRA
0 1 S582 RADTSQPSARMRGHG
0 1 S641 KALVIQESERRKKLR
0 2 - gap
0 3 - gap
0 1 Y745 VQRGGCVYSKEEDGS
0 1 K882 ANPELRNKEGDTAWD
0 1 T886 LRNKEGDTAWDLTPE
0 2 K1047 LQLYRTAKMGWGVRA
0 1 Y1085 DVREDDSYLFDLDNK
0 1 S1108 ARYYGNISRFINHLC
0 1 T1146 FSSRDIRTGEELGFD
0 2 S1187-p EAIALEQsRLARLDP
0 1 S1204 ELLPELGSLPPVNT_
0 1 T1210 GSLPPVNT_______
  EHMT2 iso2  
- gap
- gap
- gap
G39 GATERVHGSLGDtPR
S40 ATERVHGSLGDtPRS
T44-p VHGSLGDtPRSEETL
S47 SLGDtPRSEETLPKA
T50 DtPRSEETLPKATPD
S66 LEPAGPSSPASVTVT
S69 AGPSSPASVTVTVGD
P87 DTPVGATPLIGDESE
K114 ILLGHATKSFPSsPS
K114 ILLGHATKSFPSsPS
S118 HATKSFPSsPSKGGS
S119-p ATKSFPSsPSKGGSC
S121 KSFPSsPSKGGSCPS
K122 SFPSsPSKGGSCPSR
S125 SsPSKGGSCPSRAKM
S133 CPSRAKMSMTGAGKS
T135 SRAKMSMTGAGKSPP
S140 SMTGAGKSPPSVQSL
S143 GAGKSPPSVQSLAMR
S153 SLAMRLLSMPGAQGA
T171 GSEPPPATTSPEGQP
S173 EPPPATTSPEGQPKV
K185 PKVHRARKTMSKPGN
K185 PKVHRARKTMSKPGN
K189 RARKTMSKPGNGQPP
S211 EIQHFRMSDDVHSLG
S232 AKRRKLNSGGGLSEE
G235 RKLNSGGGLSEELGS
S237 LNSGGGLSEELGSAR
S242 GLSEELGSARRSGEV
S246 ELGSARRSGEVTLTK
T252 RSGEVTLTKGDPGSL
K348 KAKKKWRKDSPWVKP
S350 KKKWRKDSPWVKPSR
S356 DSPWVKPSRKRRKRE
S378 RGVSNDTSSLETERG
S379 GVSNDTSSLETERGF
T382 NDTSSLETERGFEEL
T434 AAILKRETMRPSSRV
T521 IPRGDGVTPPAGTAA
T526 GVTPPAGTAAPAPPP
S535-p APAPPPLsQDVPGRA
S548 RADTSQPSARMRGHG
S607 KALVIQESERRKKLR
- gap
- gap
Y711 VQRGGCVYSKEEDGS
K848 ANPELRNKEGDTAWD
T852 LRNKEGDTAWDLTPE
K1013 LQLYRTAKMGWGVRA
Y1051 DVREDDSYLFDLDNK
S1074 ARYYGNISRFINHLC
T1112 FSSRDIRTGEELGFD
S1153 EAIALEQSRLARLDP
S1170 ELLPELGSLPPVNT_
T1176 GSLPPVNT_______
  EHMT2 iso4  
R40 HRGRGRPRsLLsLPR
S41-p RGRGRPRsLLsLPRA
S44-p GRPRsLLsLPRAQAS
G96 GATERVHGsLGDtPR
S97-p ATERVHGsLGDtPRS
T101-p VHGsLGDtPRSEETL
S104 sLGDtPRSEETLPKA
T107 DtPRSEETLPKATPD
S123 LEPAGPSSPASVTVT
S126 AGPSSPASVTVTVGD
P144 DTPVGATPLIGDESE
K171-m2 ILLGHATkSFPssPs
K171 ILLGHATKSFPssPs
S175-p HATkSFPssPskGGs
S176-p ATkSFPssPskGGsC
S178-p kSFPssPskGGsCPS
K179-ac SFPssPskGGsCPSR
S182-p ssPskGGsCPSRAKM
S190-p CPSRAKMsMtGAGKs
T192-p SRAKMsMtGAGKsPP
S197-p sMtGAGKsPPsVQSL
S200-p GAGKsPPsVQSLAMR
S210-p SLAMRLLsMPGAQGA
T228-p GSEPPPAtTsPEGQP
S230-p EPPPAtTsPEGQPKV
K242-m2 PKVHRARkTMSkPGN
K242-m3 PKVHRARkTMSkPGN
K246-m3 RARkTMSkPGNGQPP
S268-p EIQHFRMsDDVHSLG
S289-p AKRRKLNsGGGLSEE
G292 RKLNsGGGLSEELGS
S294 LNsGGGLSEELGSAR
S299 GLSEELGSARRsGEV
S303-p ELGSARRsGEVTLtK
T309-p RsGEVTLtKGDPGSL
K405-ub KAKKKWRkDsPWVKP
S407-p KKKWRkDsPWVKPSR
S413 DsPWVKPSRKRRKRE
S435-p RGVSNDTssLETERG
S436-p GVSNDTssLETERGF
T439 NDTssLETERGFEEL
T491-p AAILKREtMRPSSRV
T578-p IPRGDGVtPPAGtAA
T583-p GVtPPAGtAAPAPPP
S592-p APAPPPLsQDVPGRA
S605 RADTSQPSARMRGHG
S664-p KALVIQEsERRKKLR
- gap
- gap
Y768 VQRGGCVYSKEEDGS
K905-ub ANPELRNkEGDTAWD
T909 LRNkEGDTAWDLTPE
K1070-ub LQLYRTAkMGWGVRA
Y1108-p DVREDDSyLFDLDNK
S1131 ARYYGNISRFINHLC
T1169 FSSRDIRTGEELGFD
S1210-p EAIALEQsRLARLDP
S1227-p ELLPELGsLPPVNt_
T1233-p GsLPPVNt_______
  mouse

► Hide Isoforms
 
R40-m2 HRGRGRPrsLLsLPR
S41-p RGRGRPrsLLsLPRA
S44-p GRPrsLLsLPRAQAS
S96-p GAAERVHsSLGDTPQ
S97 AAERVHsSLGDTPQS
T101 VHsSLGDTPQSEETL
S104 SLGDTPQSEETLPKA
T107 DTPQSEETLPKANPD
S123-p LEPAGPSsPAsVTVT
S126-p AGPSsPAsVTVTVGD
S144-p DTPVGAAsLIGDEPE
K167-m2 IVLGHATkSFPSsPS
K167-m3 IVLGHATkSFPSsPS
S171 HATkSFPSsPSKGGA
S172-p ATkSFPSsPSKGGAC
S174 kSFPSsPSKGGACPS
K175 SFPSsPSKGGACPSR
A178 SsPSKGGACPSRAKM
S186-p CPSRAKMsMtGAGKs
T188-p SRAKMsMtGAGKsPP
S193-p sMtGAGKsPPsVQSL
S196-p GAGKsPPsVQSLAMR
S206 SLAMRLLSMPGAQGA
T224 GPEPSPATTAAQEGQ
A226 EPSPATTAAQEGQPK
K239 PKVHRARKTMSKPSN
K239-m3 PKVHRARkTMSKPSN
K243 RARkTMSKPSNGQPP
S265 EVQHFRMSDDMHLGK
S285-p AKRRKLNsGsLsEDL
S287-p RRKLNsGsLsEDLGs
S289-p KLNsGsLsEDLGsAG
S294-p sLsEDLGsAGGsGDI
S298-p DLGsAGGsGDIILEK
E304 GsGDIILEKGEPRPL
K401 KAKKKWRKDSPWVKP
S403 KKKWRKDSPWVKPSR
S409 DSPWVKPSRKRRKRE
S465-p AGVSNDTssLEtERG
S466-p GVSNDTssLEtERGF
T469-p NDTssLEtERGFEEL
T521 AAILKRETMRPSSRV
T608-p IPRGDGGtPPIGtAA
T613-p GGtPPIGtAAPALPP
A622 APALPPLAHDAPGRA
S635-p RADTSQPsARMRGHG
S694 KALVIQESERRKKLR
- gap
- gap
Y798-p VQLGGCVySKEEDGS
K935 ANPELRNKEGDtAWD
T939-p LRNKEGDtAWDLTPE
K1100-ub LQLYRTAkMGWGVRA
Y1138 DVREDDSYLFDLDNK
S1161-p ARYYGNIsRFINHLC
T1199-p FSSRDIRtGEELGFD
S1240 EAIALEQSRLARLDP
S1257 ELLPDLSSLPPINT_
T1263 SSLPPINT_______
  EHMT2 iso4  
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
T32 AAILKRETMRPSSRV
T119 IPRGDGGTPPIGTAA
T124 GGTPPIGTAAPALPP
A133 APALPPLAHDAPGRA
S146 RADTSQPSARMRGHG
S205 KALVIQESESPPsPP
S210-p QESESPPsPPPsPDR
S214-p SPPsPPPsPDRRKKL
Y319 VQLGGCVYSKEEDGS
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
  rat

 
R40 HRGRGRPRSLLSLPR
S41 RGRGRPRSLLSLPRA
S44 GRPRSLLSLPRAQAS
G96 GAAERVHGSLGDTSH
S97 AAERVHGSLGDTSHS
T101 VHGSLGDTSHSEETL
S104 SLGDTSHSEETLPKA
T107 DTSHSEETLPKANPD
S123 LEPTGPSSPASVTVT
S126 TGPSSPASVTVTVGD
P144 DTPVGATPLIGEEPE
K167 ILLGHATKSFPSSPS
K167 ILLGHATKSFPSSPS
S171 HATKSFPSSPSKGGA
S172 ATKSFPSSPSKGGAC
S174 KSFPSSPSKGGACPS
K175 SFPSSPSKGGACPSR
A178 SSPSKGGACPSRAKM
S186 CPSRAKMSMTGAGKS
T188 SRAKMSMTGAGKSPP
S193 SMTGAGKSPPSVQSL
S196 GAGKSPPSVQSLAMR
S206 SLAMRLLSMPGAQGA
T224 GPEPPPATTAGQEGQ
A226 EPPPATTAGQEGQPK
K239 PKVHRARKTMSKPSN
K239 PKVHRARKTMSKPSN
K243 RARKTMSKPSNGQPP
S265 EVQHFRMSDDMHLGK
S285 AKRRKLTSGSLsEDL
S287 RRKLTSGSLsEDLGS
S289-p KLTSGSLsEDLGSAG
S294 SLsEDLGSAGGSGEV
S298 DLGSAGGSGEVILEK
E304 GSGEVILEKGEPRPL
K401 KAKKKWRKDSPWVKP
S403 KKKWRKDSPWVKPSR
S409 DSPWVKPSRKRRKRE
S465 AGVSNDTSSLETERG
S466 GVSNDTSSLETERGF
T469 NDTSSLETERGFEEL
T521 AAILKRETMRPSSRV
T608-p IPRGDGGtPPVGTVA
T613 GGtPPVGTVAPAPPP
A622 APAPPPLAHDAPGRA
S635 RADTSQPSARMRGHG
S694 KALVIQESERRKKLR
- gap
- gap
Y798 VQLGGCVYSKEEDGS
K935 ANPELRNKEGDTAWD
T939 LRNKEGDTAWDLTPE
K1100 LQLYRTAKMGWGVRA
Y1138 DVREDDSYLFDLDNK
S1161 ARYYGNISRFINHLC
T1199 FSSRDIRTGEELGFD
S1240 EAIALEQSRLARLDP
S1257 ELLPDLSSLPPINT_
T1263 SSLPPINT_______
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