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Protein Page:
CDK9 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
CDK9 a protein kinase of the CDK family. Forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. A component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. Transcriptional elongation factor and cofactor for HIV Tat protein; RNAi blocks HIV replication, and inhibitors also block varicella zoster replication. Mediates signals leading to cardiac hypertrophy. Inhibitor: Flavopiridol. Note: This description may include information from UniProtKB.
Protein type: Kinase, protein; EC 2.7.11.22; EC 2.7.11.23; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; Nuclear receptor co-regulator; CMGC group; CDK family; CDK9 subfamily; CDK/CDK9 subfamily
Cellular Component: nucleoplasm; transcription elongation factor complex; PML body; transcription elongation factor complex b; intracellular membrane-bound organelle; cytoplasm; nucleolus; nucleus
Molecular Function: RNA polymerase subunit kinase activity; protein binding; snRNA binding; DNA binding; cyclin-dependent protein kinase activity; chromatin binding; ATP binding; protein kinase activity
Biological Process: regulation of histone modification; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; viral reproduction; positive regulation of viral transcription; cell cycle; DNA repair; protein amino acid phosphorylation; regulation of DNA repair; cell proliferation; transforming growth factor beta receptor signaling pathway; RNA elongation from RNA polymerase II promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; replication fork arrest
Reference #:  P50750 (UniProtKB)
Alt. Names/Synonyms: C-2K; CDC2-related kinase; CDC2L4; CDK9; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; CTK1; Cyclin-dependent kinase 9; PITALRE; PTEFB; serine/threonine protein kinase PITALRE; Serine/threonine-protein kinase PITALRE; TAK
Gene Symbols: CDK9
Molecular weight: 42,778 Da
Basal Isoelectric point: 8.97  Predict pI for various phosphorylation states
CST Pathways:  Crosstalk between PTMs
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

CDK9

Protein Structure Not Found.

Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
transcription, altered: S175‑p, T186‑p
enzymatic activity, induced: S175‑p, T186‑p
enzymatic activity, inhibited: S175‑p
molecular association, regulation: S175‑p, T186‑p, S347‑p
protein stabilization: T186‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
1 0 - gap
1 0 - gap
0 1 - gap
0 2 - gap
0 1 - gap
0 1 - gap
1 2 - gap
1 0 S7-p _MAKQYDsVECPFCD
1 0 K21-me DEVSKYEkLAkIGQG
0 1 K24-ub SKYEkLAkIGQGTFG
1 0 K35-ac GTFGEVFkARHRKTG
1 0 K35-me GTFGEVFkARHRKTG
0 7 K35-ub GTFGEVFkARHRKTG
3 0 K44-ac RHRKTGQkVALkkVL
1 0 K48-ac TGQkVALkkVLMENE
1 0 K49-ac GQkVALkkVLMENEk
1 0 K56-ac kVLMENEkEGFPITA
0 1 K56-ub kVLMENEkEGFPITA
1 0 K68-ac ITALREIkILQLLkH
0 3 K68-ub ITALREIkILQLLkH
1 0 K74-ac IkILQLLkHENVVNL
1 0 R86-me VNLIEICrTkASPYN
0 2 K88-ub LIEICrTkASPYNRC
1 0 K127-me KFTLSEIkRVMQMLL
1 0 K127-ac KFTLSEIkRVMQMLL
0 1 R148 HRNKILHRDMkAANV
0 1 K151-ub KILHRDMkAANVLIT
1 0 K164-ac ITRDGVLkLADFGLA
0 7 K164-ub ITRDGVLkLADFGLA
3 1 S175-p FGLARAFsLAkNSQP
1 0 K178-ac ARAFsLAkNSQPNRy
0 5 K178-ub ARAFsLAkNSQPNRy
0 1 Y185-p kNSQPNRytNRVVTL
11 20 T186-p NSQPNRytNRVVTLW
0 1 Y194-p NRVVTLWyRPPELLL
0 1 K264 DNYELYEKLELVkGQ
1 0 K269-ac YEKLELVkGQKRkVk
0 1 K269-ub YEKLELVkGQKRkVk
0 3 K274-ac LVkGQKRkVkDRLKA
0 3 K276-ac kGQKRkVkDRLKAYV
0 1 Y287 KAYVRDPYALDLIDk
0 8 K294-ub YALDLIDkLLVLDPA
1 0 S317-p LNHDFFWsDPMPSDL
1 5 Y338-p HLTSMFEyLAPPRRk
1 0 K345-ac yLAPPRRkGsQItQQ
0 3 K345-ub yLAPPRRkGsQItQQ
2 77 S347-p APPRRkGsQItQQst
0 3 T350-p RRkGsQItQQstNQs
1 3 S353-p GsQItQQstNQsRNP
1 1 T354-p sQItQQstNQsRNPA
0 4 S357-p tQQstNQsRNPAttN
1 0 T362-p NQsRNPAttNQtEFE
1 0 T363-p QsRNPAttNQtEFEr
0 1 T366-p NPAttNQtEFErVF_
1 0 R370-me tNQtEFErVF_____
2549 : Phospho-CDK9 (Thr186) Antibody
  CDK9 iso2  
R8-me MQRDAPPrAPAPAPR
R8-m2 MQRDAPPrAPAPAPR
S27-p PIGAAASsGGGGGGG
S35-p GGGGGGGsGGGGGGA
S52-p APAPPGLsGtTsPRG
T54-p APPGLsGtTsPRGPG
S56-p PGLsGtTsPRGPGGG
S124 AMAKQYDSVECPFCD
K138 DEVSKYEKLAKIGQG
K141 SKYEKLAKIGQGTFG
K152 GTFGEVFKARHRKTG
K152 GTFGEVFKARHRKTG
K152 GTFGEVFKARHRKTG
K161 RHRKTGQKVALKKVL
K165 TGQKVALKKVLMENE
K166 GQKVALKKVLMENEK
K173 KVLMENEKEGFPITA
K173 KVLMENEKEGFPITA
K185 ITALREIKILQLLKH
K185 ITALREIKILQLLKH
K191 IKILQLLKHENVVNL
R203 VNLIEICRTKASPYN
K205 LIEICRTKASPYNRC
K244 KFTLSEIKRVMQMLL
K244 KFTLSEIKRVMQMLL
R265 HRNKILHRDMKAANV
K268 KILHRDMKAANVLIT
K281 ITRDGVLKLADFGLA
K281 ITRDGVLKLADFGLA
S292 FGLARAFSLAKNSQP
K295 ARAFSLAKNSQPNRY
K295 ARAFSLAKNSQPNRY
Y302 KNSQPNRYTNRVVTL
T303 NSQPNRYTNRVVTLW
Y311 NRVVTLWYRPPELLL
K381 DNYELYEKLELVKGQ
K386 YEKLELVKGQKRkVk
K386 YEKLELVKGQKRkVk
K391-ac LVKGQKRkVkDRLKA
K393-ac KGQKRkVkDRLKAYV
Y404 KAYVRDPYALDLIDK
K411 YALDLIDKLLVLDPA
S434 LNHDFFWSDPMPSDL
Y455 HLTSMFEYLAPPRRK
K462 YLAPPRRKGSQITQQ
K462 YLAPPRRKGSQITQQ
S464 APPRRKGSQITQQST
T467 RRKGSQITQQSTNQS
S470 GSQITQQSTNQSRNP
T471 SQITQQSTNQSRNPA
S474 TQQSTNQSRNPATTN
T479 NQSRNPATTNQTEFE
T480 QSRNPATTNQTEFER
T483 NPATTNQTEFERVF_
R487 TNQTEFERVF_____
  mouse

 
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S7 _MAKQYDSVECPFCD
K21 DEVTKYEKLAKIGQG
K24 TKYEKLAKIGQGTFG
K35 GTFGEVFKAKHRQTG
K35 GTFGEVFKAKHRQTG
K35 GTFGEVFKAKHRQTG
K44 KHRQTGQKVALKKVL
K48 TGQKVALKKVLMENE
K49 GQKVALKKVLMENEK
K56 KVLMENEKEGFPITA
K56 KVLMENEKEGFPITA
K68 ITALREIKILQLLKH
K68-ub ITALREIkILQLLKH
K74 IkILQLLKHENVVNL
R86 VNLIEICRTKASPYN
K88 LIEICRTKASPYNRC
K127 KFTLSEIKRVMQMLL
K127 KFTLSEIKRVMQMLL
R148-m2 HRNKILHrDMKAANV
K151 KILHrDMKAANVLIT
K164 ITRDGVLKLADFGLA
K164-ub ITRDGVLkLADFGLA
S175 FGLARAFSLAkNSQP
K178 ARAFSLAKNSQPNRY
K178-ub ARAFSLAkNSQPNRY
Y185 kNSQPNRYtNRVVTL
T186-p NSQPNRYtNRVVTLW
Y194 NRVVTLWYRPPELLL
K264-ub DKYELFEkLELVKGQ
K269 FEkLELVKGQKRKVK
K269 FEkLELVKGQKRKVK
K274 LVKGQKRKVKDRLKA
K276 KGQKRKVKDRLKAYV
Y287-p KAYVRDPyALDLIDK
K294 yALDLIDKLLVLDPA
S317 LNHDFFWSDPMPSDL
Y338 HLTSMFEYLAPPRRK
K345 YLAPPRRKGsQITQQ
K345 YLAPPRRKGsQITQQ
S347-p APPRRKGsQITQQST
T350 RRKGsQITQQSTNQS
S353 GsQITQQSTNQSRNP
T354 sQITQQSTNQSRNPA
S357 TQQSTNQSRNPATTN
T362 NQSRNPATTNQTEFE
T363 QSRNPATTNQTEFER
T366 NPATTNQTEFERVF_
R370 TNQTEFERVF_____
2549 : Phospho-CDK9 (Thr186) Antibody
  rat

 
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S7 _MAKQYDSVECPFCD
K21 DEVTKYEKLAKIGQG
K24 TKYEKLAKIGQGTFG
K35 GTFGEVFKAKHRQTG
K35 GTFGEVFKAKHRQTG
K35 GTFGEVFKAKHRQTG
K44 KHRQTGQKVALKKVL
K48 TGQKVALKKVLMENE
K49 GQKVALKKVLMENEK
K56 KVLMENEKEGFPITA
K56 KVLMENEKEGFPITA
K68 ITALREIKILQLLKH
K68 ITALREIKILQLLKH
K74 IKILQLLKHENVVNL
R86 VNLIEICRTKASPYN
K88 LIEICRTKASPYNRC
K127 KFTLSEIKRVMQMLL
K127 KFTLSEIKRVMQMLL
R148 HRNKILHRDMKAANV
K151 KILHRDMKAANVLIT
K164 ITRDGVLKLADFGLA
K164 ITRDGVLKLADFGLA
S175 FGLARAFSLAKNSQP
K178 ARAFSLAKNSQPNRY
K178 ARAFSLAKNSQPNRY
Y185 KNSQPNRYTNRVVTL
T186 NSQPNRYTNRVVTLW
Y194 NRVVTLWYRPPELLL
K264 DKYELFEKLELVKGQ
K269 FEKLELVKGQKRKVK
K269 FEKLELVKGQKRKVK
K274 LVKGQKRKVKDRLKA
K276 KGQKRKVKDRLKAYV
Y287 KAYVRDPYALDLIDK
K294 YALDLIDKLLVLDPA
S317 LNHDFFWSDPMPSDL
Y338 HLTSMFEYLAPPRRK
K345 YLAPPRRKGsQITQQ
K345 YLAPPRRKGsQITQQ
S347-p APPRRKGsQITQQST
T350 RRKGsQITQQSTNQS
S353 GsQITQQSTNQSRNP
T354 sQITQQSTNQSRNPA
S357 TQQSTNQSRNPATTN
T362 NQSRNPATTNQTEFE
T363 QSRNPATTNQTEFER
T366 NPATTNQTEFERVF_
R370 TNQTEFERVF_____
2549 : Phospho-CDK9 (Thr186) Antibody
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