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Protein Page:
ARAF (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
ARAF a tyrosine kinase-like kinase of the RAF family. Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations. Activating mutations are also seen in ~10% of colorectal cancers, in lung cancers and gliomas, and at a lower rate in several other tumors. Inactivating mutations are also seen and may result in activation of c-Raf and Erk. Mutations in B-Raf, MEK1 and MEK2 also associated with cardiofaciocutaneous syndrome, displaying morphological, cardiac and mental defects. Approved Inhibitor: Nexavar/Sorafenib. Note: This description may include information from UniProtKB.
Protein type: Kinase, protein; Protein kinase, TKL; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); TKL group; RAF family
Cellular Component: mitochondrion
Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; receptor signaling protein activity; ATP binding; protein kinase activity
Biological Process: regulation of proteasomal ubiquitin-dependent protein catabolic process; protein modification process; positive regulation of peptidyl-serine phosphorylation; regulation of TOR signaling pathway; negative regulation of apoptosis
Reference #:  P10398 (UniProtKB)
Alt. Names/Synonyms: A-RAF; A-Raf proto-oncogene serine/threonine-protein kinase; ARAF; ARAF1; Oncogene ARAF1; PKS; PKS2; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks; RAFA1; Ras-binding protein DA-Raf; Serine/threonine-protein kinase A-Raf; v-raf murine sarcoma 3611 viral oncogene homolog; v-raf murine sarcoma 3611 viral oncogene homolog 1
Gene Symbols: ARAF
Molecular weight: 67,585 Da
Basal Isoelectric point: 9.2  Predict pI for various phosphorylation states
CST Pathways:  ErbB/HER Signaling  |  ESC Pluripotency and Differentiation  |  IL6 Signaling  |  Inhibition of Apoptosis  |  T Cell Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

ARAF

Protein Structure Not Found.


STRING  |  Scansite  |  KinBase  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho3D  |  Source  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene


Sites Implicated In
activity, induced: Y301‑p, Y302‑p
enzymatic activity, induced: S214‑p, S257‑p, S262‑p, S264‑p, S432‑p, T442‑p, T452‑p, T455‑p, S582‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S15-p PANGAEPsRAVGtVK
0 1 T20-p EPsRAVGtVKVyLPN
0 1 Y24-p AVGtVKVyLPNKQRT
0 3 Y42-p VRDGMSVyDSLDKAL
0 32 Y155-p STNRQQFyHsVQDLs
0 23 S157-p NRQQFyHsVQDLsGG
0 1 S162-p yHsVQDLsGGSRQHE
0 24 T181-p RPLNELLtPQGPsPR
0 51 S186-p LLtPQGPsPRTQHCD
0 1 T189 PQGPsPRTQHCDPEH
0 6 S212-p APLQRIRststPNVH
0 5 T213-p PLQRIRststPNVHM
1 25 S214-p LQRIRststPNVHMV
0 27 T215-p QRIRststPNVHMVs
0 2 S222-p tPNVHMVsttAPMDs
0 2 T223-p PNVHMVsttAPMDsN
0 2 T224-p NVHMVsttAPMDsNL
0 3 S229-p sttAPMDsNLIQLTG
0 4 S250-p AAGSRGGsDGtPRGs
0 13 T253-p SRGGsDGtPRGsPsP
2 57 S257-p sDGtPRGsPsPAsVs
1 3 S259-p GtPRGsPsPAsVssG
1 1 S262-p RGsPsPAsVssGRKs
1 1 S264-p sPsPAsVssGRKsPH
0 2 S265-p PsPAsVssGRKsPHs
0 3 S269-p sVssGRKsPHsKsPA
0 2 S272-p sGRKsPHsKsPAEQR
0 1 S274-p RKsPHsKsPAEQRER
0 5 S283-p AEQRERKsLADDKKK
0 5 Y296-p KKVKNLGyRDsGyyW
1 6 S299-p KNLGyRDsGyyWEVP
1 11 Y301-p LGyRDsGyyWEVPPS
1 94 Y302-p GyRDsGyyWEVPPSE
0 2 K314-u PSEVQLLkRIGtGsF
0 11 T318-p QLLkRIGtGsFGtVF
0 1 S320-p LkRIGtGsFGtVFRG
0 1 T323-p IGtGsFGtVFRGRWH
0 1 S341-p AVKVLKVsQPtAEQA
0 1 T344-p VLKVsQPtAEQAQAF
0 1 T413-p LIDVARQtAQGMDyL
0 4 Y419-p QtAQGMDyLHAKNII
1 1 S432-p IIHRDLKsNNIFLHE
0 1 T442-p IFLHEGLtVKIGDFG
0 1 T452-p IGDFGLAtVktRWsG
0 3 K454-u DFGLAtVktRWsGAQ
0 2 T455-p FGLAtVktRWsGAQP
0 1 S458-p AtVktRWsGAQPLEQ
0 1 Y526-p IFMVGRGyLsPDLsK
0 1 S528-p MVGRGyLsPDLsKIs
0 1 S532-p GyLsPDLsKIssNCP
0 1 S535-p sPDLsKIssNCPkAM
0 1 S536-p PDLsKIssNCPkAMR
0 2 K540-u KIssNCPkAMRRLLs
0 1 S547-p kAMRRLLsDCLKFQR
0 1 S573-p TIELLQRsLPKIERs
0 6 S580-p sLPKIERsAsEPsLH
1 39 S582-p PKIERsAsEPsLHRt
0 3 S585-p ERsAsEPsLHRtQAD
0 2 T589-p sEPsLHRtQADELPA
0 1 S600-p ELPACLLsAARLVP_
4431 : Phospho-A-Raf (Ser299) Antibody
  mouse

 
S15 PVSGAEPSRAVGTVK
T20 EPSRAVGTVKVYLPN
Y24 AVGTVKVYLPNKQRT
Y42 VREGMSVYDSLDKAL
Y155-p STNRRQFyHsIQDLS
S157-p NRRQFyHsIQDLSGG
S162 yHsIQDLSGGSRQQE
T181-p LSVNELLtPQGPsPF
S186-p LLtPQGPsPFTQQRD
T189 PQGPsPFTQQRDQEH
S212-p PPLQRIRsTstPNVH
T213 PLQRIRsTstPNVHM
S214-p LQRIRsTstPNVHMV
T215-p QRIRsTstPNVHMVs
S222-p tPNVHMVsttAPMDs
T223-p PNVHMVsttAPMDsS
T224-p NVHMVsttAPMDsSL
S229-p sttAPMDsSLMQFTA
- gap
A251 AGRGGDGAPRGsPSP
S255-p GDGAPRGsPSPASVS
S257 GAPRGsPSPASVSsG
S260 RGsPSPASVSsGRKS
S262 sPSPASVSsGRKSPH
S263-p PSPASVSsGRKSPHS
S267 SVSsGRKSPHSKLPS
S270 sGRKSPHSKLPSEQR
L272 RKSPHSKLPSEQRER
S281 SEQRERKSLADEKKK
Y294-p KKVKNLGyRDSGyyW
S297 KNLGyRDSGyyWEVP
Y299-p LGyRDSGyyWEVPPS
Y300-p GyRDSGyyWEVPPSE
K312 PSEVQLLKRIGTGSF
T316 QLLKRIGTGSFGTVF
S318 LKRIGTGSFGTVFRG
T321 IGTGSFGTVFRGRWH
A339 AVKVLKVAQPTAEQA
T342 VLKVAQPTAEQAQAF
T411 LIDVARQTAQGMDYL
Y417 QTAQGMDYLHAKNII
S430 IIHRDLKSNNIFLHE
T440 IFLHEGLTVKIGDFG
T450 IGDFGLATVKTRWSG
K452 DFGLATVKTRWSGAQ
T453 FGLATVKTRWSGAQP
S456 ATVKTRWSGAQPLEQ
Y524 IFMVGRGYLSPDLSK
S526 MVGRGYLSPDLSKIF
S530 GYLSPDLSKIFSNCP
F533 SPDLSKIFSNCPKAM
S534 PDLSKIFSNCPKAMR
K538 KIFSNCPKAMRRLLT
T545 KAMRRLLTDCLKFQR
S571 TIELLQRSLPKIERs
S578-p SLPKIERsAsEPsLH
S580-p PKIERsAsEPsLHRT
S583-p ERsAsEPsLHRTQAD
T587 sEPsLHRTQADELPA
S598 ELPACLLSAARLVP_
4431 : Phospho-A-Raf (Ser299) Antibody
  rat

 
S15 PASGAEPSRAVGTVK
T20 EPSRAVGTVKVYLPN
Y24 AVGTVKVYLPNKQRT
Y42-p VRDGMSVyDSLDKAL
Y155 STNRRQFYHSIQDLS
S157 NRRQFYHSIQDLSGG
S162 YHSIQDLSGGSRQQE
T181-p LSVNELLtPQGPsPF
S186-p LLtPQGPsPFtQQRD
T189-p PQGPsPFtQQRDQEH
S212 PPLQRIRSTSTPNVH
T213 PLQRIRSTSTPNVHM
S214 LQRIRSTSTPNVHMV
T215 QRIRSTSTPNVHMVS
S222 TPNVHMVSTTAPMDS
T223 PNVHMVSTTAPMDSS
T224 NVHMVSTTAPMDSSL
S229 STTAPMDSSLMQFTA
- gap
A251 AGRGGDGAPRGSPSP
S255 GDGAPRGSPSPASVS
S257 GAPRGSPSPASVSSG
S260 RGSPSPASVSSGRKS
S262 SPSPASVSSGRKSPH
S263 PSPASVSSGRKSPHS
S267 SVSSGRKSPHSKLPA
S270 SGRKSPHSKLPAEQR
L272 RKSPHSKLPAEQRER
S281 AEQRERKSLADEKKK
Y294 KKVKNLGYRDSGYYW
S297 KNLGYRDSGYYWEVP
Y299 LGYRDSGYYWEVPPS
Y300 GYRDSGYYWEVPPSE
K312 PSEVQLLKRIGTGSF
T316 QLLKRIGTGSFGTVF
S318 LKRIGTGSFGTVFRG
T321 IGTGSFGTVFRGRWH
A339 AVKVLKVAQPTAEQA
T342 VLKVAQPTAEQAQAF
T411 LIDVARQTAQGMDYL
Y417 QTAQGMDYLHAKNII
S430 IIHRDLKSNNIFLHE
T440 IFLHEGLTVKIGDFG
T450 IGDFGLATVKTRWSG
K452 DFGLATVKTRWSGAQ
T453 FGLATVKTRWSGAQP
S456 ATVKTRWSGAQPLEQ
Y524 IFMVGRGYLSPDLSK
S526 MVGRGYLSPDLSKIF
S530 GYLSPDLSKIFSNCP
F533 SPDLSKIFSNCPKAM
S534 PDLSKIFSNCPKAMR
K538 KIFSNCPKAMRRLLT
T545 KAMRRLLTDCLKFQR
S571 TIELLQRSLPKIERS
S578 SLPKIERSAsEPSLH
S580-p PKIERSAsEPSLHRT
S583 ERSAsEPSLHRTQAD
T587 sEPSLHRTQADELPA
S598 ELPACLLSAARLVP_
4431 : Phospho-A-Raf (Ser299) Antibody
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