Home

A-Raf (human)

Overview A-Raf a tyrosine kinase-like kinase of the RAF family. Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations. Activating mutations are also seen in ~10% of colorectal cancers, in lung cancers and gliomas, and at a lower rate in several other tumors. Inactivating mutations are also seen and may result in activation of c-Raf and Erk. Mutations in B-Raf, MEK1 and MEK2 also associated with cardiofaciocutaneous syndrome, displaying morphological, cardiac and mental defects. Approved Inhibitor: Nexavar/Sorafenib. Note: This description may include information from UniProtKB. Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, TKL; TKL group; RAF family Cellular Component: mitochondrion; cytosol Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; receptor signaling protein activity; ATP binding; protein kinase activity Biological Process: protein modification process; positive regulation of peptidyl-serine phosphorylation; protein amino acid phosphorylation; negative regulation of apoptosis Reference #:  P10398 (UniProtKB) Alt. Names/Synonyms: A-RAF; A-Raf proto-oncogene serine/threonine-protein kinase; ARAF; ARAF1; Oncogene ARAF1; PKS; PKS2; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks; RAFA1; Ras-binding protein DA-Raf; Serine/threonine-protein kinase A-Raf; v-raf murine sarcoma 3611 viral oncogene homolog; v-raf murine sarcoma 3611 viral oncogene homolog 1 Gene Symbols: ARAF Molecular weight: 67,585 Da Basal Isoelectric point: 9.2  Predict pI for various phosphorylation states CST Pathways:  ErbB/HER Signaling  |  ESC Pluripotency and Differentiation  |  Inhibition of Apoptosis  |  Jak/Stat/IL-6 Signaling  |  T Cell Receptor Signaling Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below A-Raf 1WXM - A=19-91 (human)

STRING  |  Scansite  |  KinBase  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho3D  |  DISEASE  |  Source  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene

	Sites Implicated In
activation: Y301‑p, Y302‑p enzymatic activity, induced: S214‑p, S257‑p, S262‑p, S264‑p, S432‑p, T442‑p, T452‑p, T455‑p, S582‑p

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
0	1		S15-p	PANGAEPsRAVGtVK
0	1		T20-p	EPsRAVGtVKVyLPN
0	1		Y24-p	AVGtVKVyLPNKQRT
0	3		Y42-p	VRDGMSVyDSLDKAL
0	32		Y155-p	STNRQQFyHsVQDLS
0	21		S157-p	NRQQFyHsVQDLSGG
0	22		T181-p	RPLNELLtPQGPsPR
0	50		S186-p	LLtPQGPsPRTQHCD
0	1		T189	PQGPsPRTQHCDPEH
0	6		S212-p	APLQRIRststPNVH
0	5		T213-p	PLQRIRststPNVHM
1	24		S214-p	LQRIRststPNVHMV
0	26		T215-p	QRIRststPNVHMVs
0	1		S222-p	tPNVHMVsttAPMDs
0	1		T223-p	PNVHMVsttAPMDsN
0	1		T224-p	NVHMVsttAPMDsNL
0	1		S229-p	sttAPMDsNLIQLTG
0	4		S250-p	AAGSRGGsDGtPRGs
0	12		T253-p	SRGGsDGtPRGsPsP
2	56		S257-p	sDGtPRGsPsPAsVs
1	3		S259-p	GtPRGsPsPAsVssG
1	1		S262-p	RGsPsPAsVssGRKs
1	1		S264-p	sPsPAsVssGRKsPH
0	2		S265-p	PsPAsVssGRKsPHs
0	2		S269-p	sVssGRKsPHsKsPA
0	2		S272-p	sGRKsPHsKsPAEQR
0	1		S274-p	RKsPHsKsPAEQRER
0	5		S283-p	AEQRERKsLADDKKK
0	5		Y296-p	KKVKNLGyRDsGyyW
1	6		S299-p	KNLGyRDsGyyWEVP
1	11		Y301-p	LGyRDsGyyWEVPPS
1	93		Y302-p	GyRDsGyyWEVPPSE
0	2		K314-u	PSEVQLLkRIGtGsF
0	11		T318-p	QLLkRIGtGsFGtVF
0	1		S320-p	LkRIGtGsFGtVFRG
0	1		T323-p	IGtGsFGtVFRGRWH
0	1		S341-p	AVKVLKVsQPtAEQA
0	1		T344-p	VLKVsQPtAEQAQAF
0	1		T413-p	LIDVARQtAQGMDyL
0	4		Y419-p	QtAQGMDyLHAKNII
1	1		S432-p	IIHRDLKsNNIFLHE
0	1		T442-p	IFLHEGLtVKIGDFG
0	1		T452-p	IGDFGLAtVktRWsG
0	3		K454-u	DFGLAtVktRWsGAQ
0	2		T455-p	FGLAtVktRWsGAQP
0	1		S458-p	AtVktRWsGAQPLEQ
0	1		Y526-p	IFMVGRGyLsPDLsK
0	1		S528-p	MVGRGyLsPDLsKIs
0	1		S532-p	GyLsPDLsKIssNCP
0	1		S535-p	sPDLsKIssNCPkAM
0	1		S536-p	PDLsKIssNCPkAMR
0	2		K540-u	KIssNCPkAMRRLLs
0	1		S547-p	kAMRRLLsDCLKFQR
0	1		S573-p	TIELLQRsLPKIERs
0	6		S580-p	sLPKIERsAsEPsLH
1	32		S582-p	PKIERsAsEPsLHRt
0	3		S585-p	ERsAsEPsLHRtQAD
0	2		T589-p	sEPsLHRtQADELPA
0	1		S600-p	ELPACLLsAARLVP_

	mouse
S15	PVSGAEPSRAVGTVK
T20	EPSRAVGTVKVYLPN
Y24	AVGTVKVYLPNKQRT
Y42	VREGMSVYDSLDKAL
Y155-p	STNRRQFyHsIQDLS
S157-p	NRRQFyHsIQDLSGG
T181-p	LSVNELLtPQGPsPF
S186-p	LLtPQGPsPFTQQRD
T189	PQGPsPFTQQRDQEH
S212-p	PPLQRIRsTStPNVH
T213	PLQRIRsTStPNVHM
S214	LQRIRsTStPNVHMV
T215-p	QRIRsTStPNVHMVs
S222-p	tPNVHMVstTAPMDS
T223-p	PNVHMVstTAPMDSS
T224	NVHMVstTAPMDSSL
S229	stTAPMDSSLMQFTA
-	gap
A251	AGRGGDGAPRGsPSP
S255-p	GDGAPRGsPSPASVS
S257	GAPRGsPSPASVSsG
S260	RGsPSPASVSsGRKS
S262	sPSPASVSsGRKSPH
S263-p	PSPASVSsGRKSPHS
S267	SVSsGRKSPHSKLPS
S270	sGRKSPHSKLPSEQR
L272	RKSPHSKLPSEQRER
S281	SEQRERKSLADEKKK
Y294-p	KKVKNLGyRDSGyyW
S297	KNLGyRDSGyyWEVP
Y299-p	LGyRDSGyyWEVPPS
Y300-p	GyRDSGyyWEVPPSE
K312	PSEVQLLKRIGTGSF
T316	QLLKRIGTGSFGTVF
S318	LKRIGTGSFGTVFRG
T321	IGTGSFGTVFRGRWH
A339	AVKVLKVAQPTAEQA
T342	VLKVAQPTAEQAQAF
T411	LIDVARQTAQGMDYL
Y417	QTAQGMDYLHAKNII
S430	IIHRDLKSNNIFLHE
T440	IFLHEGLTVKIGDFG
T450	IGDFGLATVKTRWSG
K452	DFGLATVKTRWSGAQ
T453	FGLATVKTRWSGAQP
S456	ATVKTRWSGAQPLEQ
Y524	IFMVGRGYLSPDLSK
S526	MVGRGYLSPDLSKIF
S530	GYLSPDLSKIFSNCP
F533	SPDLSKIFSNCPKAM
S534	PDLSKIFSNCPKAMR
K538	KIFSNCPKAMRRLLT
T545	KAMRRLLTDCLKFQR
S571	TIELLQRSLPKIERS
S578	SLPKIERSAsEPSLH
S580-p	PKIERSAsEPSLHRT
S583	ERSAsEPSLHRTQAD
T587	sEPSLHRTQADELPA
S598	ELPACLLSAARLVP_

	rat
S15	PASGAEPSRAVGTVK
T20	EPSRAVGTVKVYLPN
Y24	AVGTVKVYLPNKQRT
Y42-p	VRDGMSVyDSLDKAL
Y155	STNRRQFYHSIQDLS
S157	NRRQFYHSIQDLSGG
T181-p	LSVNELLtPQGPsPF
S186-p	LLtPQGPsPFtQQRD
T189-p	PQGPsPFtQQRDQEH
S212	PPLQRIRSTSTPNVH
T213	PLQRIRSTSTPNVHM
S214	LQRIRSTSTPNVHMV
T215	QRIRSTSTPNVHMVS
S222	TPNVHMVSTTAPMDS
T223	PNVHMVSTTAPMDSS
T224	NVHMVSTTAPMDSSL
S229	STTAPMDSSLMQFTA
-	gap
A251	AGRGGDGAPRGSPSP
S255	GDGAPRGSPSPASVS
S257	GAPRGSPSPASVSSG
S260	RGSPSPASVSSGRKS
S262	SPSPASVSSGRKSPH
S263	PSPASVSSGRKSPHS
S267	SVSSGRKSPHSKLPA
S270	SGRKSPHSKLPAEQR
L272	RKSPHSKLPAEQRER
S281	AEQRERKSLADEKKK
Y294	KKVKNLGYRDSGYYW
S297	KNLGYRDSGYYWEVP
Y299	LGYRDSGYYWEVPPS
Y300	GYRDSGYYWEVPPSE
K312	PSEVQLLKRIGTGSF
T316	QLLKRIGTGSFGTVF
S318	LKRIGTGSFGTVFRG
T321	IGTGSFGTVFRGRWH
A339	AVKVLKVAQPTAEQA
T342	VLKVAQPTAEQAQAF
T411	LIDVARQTAQGMDYL
Y417	QTAQGMDYLHAKNII
S430	IIHRDLKSNNIFLHE
T440	IFLHEGLTVKIGDFG
T450	IGDFGLATVKTRWSG
K452	DFGLATVKTRWSGAQ
T453	FGLATVKTRWSGAQP
S456	ATVKTRWSGAQPLEQ
Y524	IFMVGRGYLSPDLSK
S526	MVGRGYLSPDLSKIF
S530	GYLSPDLSKIFSNCP
F533	SPDLSKIFSNCPKAM
S534	PDLSKIFSNCPKAMR
K538	KIFSNCPKAMRRLLT
T545	KAMRRLLTDCLKFQR
S571	TIELLQRSLPKIERS
S578	SLPKIERSAsEPSLH
S580-p	PKIERSAsEPSLHRT
S583	ERSAsEPSLHRTQAD
T587	sEPSLHRTQADELPA
S598	ELPACLLSAARLVP_

Home  |  Curator Login With enhanced literature mining using Linguamatics I2E Produced by 3rd Millennium  |  Design by Digizyme ©2003-2013 Cell Signaling Technology, Inc.